Despite efforts to intervene, hamstring muscle injuries (HMI) continue to be one of the largest epidemiological burdens in professional football. The injury mechanism takes place dominantly during sprinting, but also other scenarios have been observed, such as overstretching actions, jumps, and change of directions. The main biomechanical roles of the hamstring muscles are functioning as an accelerator of center-of-mass (i.e., contributing to horizontal force production), and stabilizing the pelvis and knee joint. Multiple extrinsic and intrinsic risk factors have been identified, portraying the multifactorial nature of the HMI. Furthermore, these risk factors can vary substantially between players, portraying the importance of individualized approaches. However, there is a lack of multifactorial and individualized approaches assessed for validity in literature. Thus, the overarching aim of this doctoral thesis was to explore if a specific multifactorial and individualized approach can improve upon the ongoing HMI risk reduction protocols, and thus, further reduce the HMI risk in professional football players. This was done following the Team-sport Injury Prevention model (TIP model), where the target is to evaluate the current injury burden, identify possible solutions, and intervene. The thesis comprised of three themes within professional football, I) evaluating and identifying HMI risk (completed via assessing the current epidemiological HMI situation and the association between HMI injuries and a novel hamstring screening protocol), II) improving horizontal force capacity (completed via testing if maximal theoretical horizontal force (F0) can be improved via heavy resisted sprint training), and III) developing and conducting a multifactorial and individualized training for HMI risk reduction (completed via introducing and conducting a training intervention). The conclusions from theme I were that the HMI burden continues to be high (14.1 days absent per 1000 hours of football exposure), no tests from the screening protocol were associated with an increased HMI risk when including all injuries from the season (n = 17, p > 0.05), and that lower F0 was significantly associated with increased HMI risk when including injuries between test rounds one and two (~90 days, n =14, hazard ratio: 4.02 (CI95% 1.08 to 15.0), p = 0.04). For theme II, the players initial pre-season level of F0 was significantly associated with adaptation potential after 11 weeks of heavy resisted sprint training during the pre-season (r = -0.59, p < 0.05). The heavy resisted sprint load leading to a ~50% velocity loss induced the largest improvements in sprint mechanical output and sprint performance variables. For theme III, no intervention results could be presented within this document due to the Covid-19 pandemic leading to the intervention being postponed. However, a protocol paper was published, describing in detail the intervention approach that will be used outside the scope of the thesis. In future studies, larger sample size studies are needed to support the development of more advanced HMI risk reduction models. Such models may allow practitioners to identify risk on an individual level instead of a group level. Furthermore, constant development of more specific, reliable, and accessible risk assessment tests should be promoted that can be frequently tested throughout the football season. Finally, based on the results of theme II, individualization of a specific training stimulus should be promoted in team settings.