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Abstract

Massive accumulation of the secondary ketokarotenoid astaxanthin is a characteristic stress response of certain microalgal species with Haematococcus pluvialis as an illustrious example. The carotenogenic response confers these organisms a remarkable ability to survive in extremely unfavorable environments and makes them the richest source of natural astaxanthin. Exerting a plethora of beneficial effects on human and animal health, astaxanthin is among the most important bioproducts from microalgae. Though our understanding of astaxanthin biosynthesis, induction, and regulation is far from complete, this gap is filling rapidly with new knowledge generated predominantly by application of advanced "omics" approaches. This review focuses on the most recent progress in the biology of astaxanthin accumulation in microalgae including the genomic, proteomic, and metabolomics insights into the induction and regulation of secondary carotenogenesis and its role in stress tolerance of the photosynthetic microorganisms. Special attention is paid to the coupling of the carotenoid and lipid biosynthesis as well as deposition of astaxanthin in the algal cell. The place of the carotenogenic response among the stress tolerance mechanisms is revisited, and possible implications of the new findings for biotechnological production of astaxanthin from microalgae are considered. The potential use of the carotenogenic microalgae as a source not only of value-added carotenoids, but also of biofuel precursors is discussed.
1
Alexei Solovchenko
Recent breakthroughs in the biology of astaxanthin accumulation by
microalgal cell
Department of Bioengineering, Faculty of Biology, Lomonosov Moscow State University, Moscow 119234, Russia
e-mail: solovchenko@mail.bio.msu.ru
Tel. +7(495)9392587
Fax +7(495)9394309
Abstract Massive accumulation of the secondary ketokarotenoid astaxanthin is a characteristic stress response of
certain microalgal species with Haematococcus pluvialis as an illustrious example. The carotenogenic response
confers these organisms a remarkable ability to survive in extremely unfavorable environments and makes them the
richest source of natural astaxanthin. Exerting a plethora of beneficial effects on human and animal health,
astaxanthin is among the most important bioproducts from microalgae. Though our understanding of astaxanthin
biosynthesis, its induction and regulation is far from complete, this gap is filling rapidly with new knowledge
generated predominantly by application of advanced omics approaches. This review focuses on the most recent
progress in the biology of astaxanthin accumulation in microalgae including the genomic, proteomic and
metabolomics insights into the induction and regulation of secondary carotenogenesis and its role in stress tolerance
of the photosynthetic microorganisms. Special attention is paid to the coupling of the carotenoid and lipid
biosynthesis as well as deposition of astaxanthin in the algal cell. The place of the carotenogenic response among
the stress tolerance mechanisms is revisited and possible implications of the new findings for biotechnological
production of astaxanthin from microalgae are considered. The potential use of the carotenogenic microalgae as a
source not only of value-added carotenoids, but also of biofuel precursors is discussed.
Keywords carotenogenesis, expression, kartocarotenoids, photooxidative stress, systems stress response
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Introduction
Massive accumulation of the secondary ketokarotenoid astaxanthin (Ast) is a characteristic stress response of certain
microalgal species (Takaichi 2011) with Haematococcus pluvialis (Chlorophyceae) (Sussela and Toppo 2006) as the
most illustrious example though this pigment also synthesized by plants, fungi, and bacteria (Goodwin 1961;
Johnson and Schroeder 1995). The microalgae capable of massive (up to 45% of cell dry weight, DW)
accumulation of carotenoids (Car) under stressful conditions, including Ast, are termed as carotenogenic microalgae.
In particular, H. pluvialis accumulates up to 35% DW Ast (for a comprehensive account of the biology of
H. pluvialis, reader is referred to the recent reviews by Han (Han et al. 2013a; Han et al. 2013b) and to the seminal
works referenced therein). Astaxanthin is a secondary Car meaning that it is not functionally or structurally coupled
to the photosynthetic apparatus (Lemoine and Schoefs 2010; Solovchenko 2013). Accordingly, it does not
participate in light harvesting and is exclusively involved in the protection of the algal cells under stressful
conditions. The carotenogenic response confers these organisms a remarkable ability to withstand extremely
unfavorable environmental conditions and makes them the richest source of natural Ast (Lorenz and Cysewski 2000;
Sussela and Toppo 2006; Varshney et al. 2014).
Molecule of Ast features an extensive system of 13 conjugated double bonds making it the most powerful
natural antioxidant (Guerin et al. 2003) though it is not yet clear to which extent the antioxidative effects of Ast are
exerted in vivo in the miroalgal cell. At the same time, Ast does not exert a prooxidant effect typical of other
carotenoids (Otani 2013). Furthermore, Ast is not a vitamin precursor hence it’s overdose does not pose the threat of
hypervitaminosis. Exerting a plethora of beneficial effects on human and animal health (for a recent review, see
(Yuan et al. 2011)), Ast is widely applied as a nutraceutical, pharmaceutical, safe colorant and aquaculture feed
additive (Lorenz and Cysewski 2000; Sussela and Toppo 2006). Reportedly, Ast is the most important Car
biotechnologically produced; a number of excellent reviews were dedicated to the commercial aspects of Ast
production from microalgae (see e.g. (Han et al. 2013b; Leu and Boussiba 2014; Lorenz and Cysewski 2000) and
references therein).
Molecule of Ast has two asymmetric carbon atoms at the positions 3 and 3′ of the ionone rings on either
end of the molecule. Depending on the hydroxyl groups attached to these carbon atoms, the three possible
enantiomers of Ast are designated R,R (both OH groups are above the plane of the molecule), S,S (both of the OH
groups are below the plane of the molecule) and R,S (meso-form). The Ast in H. pluvialis has optically pure
(3S,3′S)-chirality (Renstrom and Liaaen-Jensen 1981) whereas synthetic Ast is a mixture (Su et al. 2014; Wang et
al. 2014) of all three isomers containing only 25% of the biologically active isomers (Yuan and Chen 1998).
Nevertheless, over 95% of the feed market consumes synthetic Ast, which is much more affordable (Johnson and
Schroeder 1995; Lorenz and Cysewski 2000; Minyuk et al. 2008). On the other hand, the use of the synthetic
pigments, especially in food additives and pharmaceuticals, is less desirable. Therefore natural Ast remains among
the key bioproducts from microalgae despite its high price (generally, ca. 34 times higher in comparison with the
synthetic Ast) (Guerin et al. 2003; Han et al. 2013a; Lorenz and Cysewski 2000).
Characteristic responses of individual biosynthetic pathways (e.g. those for Car or fatty acids, FA) to the
stresses promoting the carotenogenesis are relatively well studied (for recent reviews, see (Boussiba 2000; Wang et
al. 2014)). Still, our understanding of the complex role of the carotenogenic response in stress tolerance of
microalgae is incomplete. This is particularly true for early events preceding the massive accumulation of Ast and
other manifestations of the acclimation of the carotenogenic microalgae to the stresses. These events involve the
sensing of the stress imposed by and the protection of the cell from the buildup of harmful reactive oxygen species
3
(ROS), transient changes in respiration, photosynthetic fixation and partitioning of carbon between different
metabolite pools and cell compartments.
The last several years were marked by explosive growth of the interest to the systems biology of Ast
accumulation, orchestration of the pigment biosynthesis by and its integration into the cell metabolic network under
the stress. A number of detailed reports became available dissecting the metabolic changes accompanying massive
accumulation of Ast under high light and nitrogen starvation (Chen et al. 2015; Gwak et al. 2014; Recht et al. 2014).
As a result, the gaps in our understanding are filling rapidly with new knowledge generated predominantly by
application of the advanced omics approaches. This review focuses on the most recent progress in the physiology
and systems biology of the Ast accumulation in the carotenogenic microalgae with primary emphasis on the most
biotechnologically important species, H. pluvialis (thought other promising microalgae such as Chlorella
(Chromochloris) zofingiensis are also briefly considered). The role of Ast accumulation in stress tolerance of the
photosynthetic microorganisms is revisited with the novel genomic, metabolomics, lipidomic and proteomic insights
in mind. We also consider possible implications of the new findings for biotechnological production of Ast from
microalgae and for exploiting the enhanced lipid accumulation tightly coupled with the carotenogenic response as a
potential source of biofuel precursors.
Astaxanthin accumulation serves multi-level stress protection
Evidently, acclimation to and protection against different stresses is served in the “green” (essentially Ast-free
motile vegetative cells), “brown” and “red” cells (immotile Ast-rich cells, the difference between the latter two will
be elaborated on below) of the carotenogenic microalgae by significantly different sets of mechanisms. Thus, the
acclimation of photosynthesis in the green cells of H. pluvialis is accomplished, like in most chlorophyte or higher
plant cells, with participation of light harvesting regulation, nonphotochemical quenching and enzymatic ROS
elimination systems (Demmig-Adams et al. 2012; Foyer and Shigeoka 2011; Horton 2014). However, these systems
are able to cope only with a stress of a limited intensity hence the green cells are more susceptible to the
photooxidative damage (Han et al. 2012; Solovchenko 2011).
The transformation of the “green” cells to non-motile palmelloid cells featuring a sizeable amount of Ast on
the background of still significant Chl content (so called “brown” cells) and then to the “red” cells is accompanied
by a considerable remodeling of the cell protection from (photo)oxidative stress. Consequently, the “brown” cells
became generally more tolerant to high light and its combinations with other stresses in comparison with the “green”
cells. As discussed by Wang et al. (2014), the higher stress tolerance of the “brown” cells is determined by different
factors (see Fig. 1). First, the “brown” cells are more efficient at avoiding the photosynthetic electron transport chain
over-reduction by channeling the excessive photosynthates to the biosynthesis of storage compounds (carbohydrates
and eventually neutral lipids (Recht et al. 2012)) and Ast. Second, the linear electron transport in these cells is
down-regulated, e.g. by decreasing the level of cytochrome b
6
f which may become very low in the “red cells”.
Third, the excess electron flow generated by PSII can be diverted to a significantly enhanced plastid terminal
oxidase (PTOX) or chlororespiration pathway. Accordingly, the important components of the thylakoid membrane
including polar lipids and the PSII proteins (particularly, D1 and PsbO) remained relatively stable in the “brown”
cells subjected to high light (Han et al. 2012; Wang et al. 2014).
Still, the cells which did not manage to accumulate sufficient amounts of Ast and retained a sizeable pool
of chlorophyll and primary Car may suffer from abrupt and/or prolonged exposition to a stress (e.g. high light, N or
4
P starvation); this is not the case in the “red” cells possessing high Ast content (Gu et al. 2013; Solovchenko 2011).
This circumstance has important consequences for biotechnological production of Ast from microalgae. A low stress
tolerance of the green cells might decline severely the Ast productivity of H. pluvialis cultures at the red stage
due to a high cell mortality (Wang et al. 2014). Accordingly, Wang et al. (2014) suggested that exposing the
palmella cells instead of “green” cells to the stress conditions may considerably increase Ast and lipid production in
H. pluvialis cultures.
Evidently, the transition from the model of photoprotection characteristic of the “green” cells and attaining
a high level of stress tolerance is complete only after the formation of Ast-rich “red” cells also called haematocysts.
The effects of Ast on the microalgal cell tolerance to different environmental stresses have been studied for more
than 20 years so there is a large body of experimental evidence accumulated in the literature, mostly for H. pluvialis
(see e.g. (Hagen et al. 1994; Hagen et al. 1993b; Lemoine and Schoefs 2010; Li et al. 2008a; Wang et al. 2003) and
references therein). It is generally accepted now that Ast accumulation in the course of the carotenogenic response
vastly increases cell tolerance to adverse environmental conditions (Boussiba 2000; Lemoine and Schoefs 2010;
Wang et al. 2003) but the exact role of Ast in this process is vigorously debated until now. Taken together, the
currently available evidence allows to distinguish at least four mechanisms of cell protection by Ast accumulation
and metabolic rearrangements (Fig. 1) accompanying this process.
Firstly, Ast is believed to act as an internal sunscreen absorbing excessive light and shielding the
chloroplast and other vulnerable cell structures from photoxidative damage (Hagen et al. 1994). Indeed, Ast was
shown to be able to intercept in vivo a considerable part of light otherwise reaching chlorophyll, and the degree of its
protection is tightly related with the ratio of the screen (Ast) and the photosensitizer (chlorophyll) in the cell (Hagen
et al. 1994; Han et al. 2012; Solovchenko 2011; Solovchenko et al. 2013). Furthermore, the significance of light
screening by the lipid droplets (LD) containing Ast was further supported by the phenomenon of quick migration of
the LD to the periphery of cell in the H. pluvialis cells exposed to high light (Peled et al. 2012).
Secondly, the Ast-containing LD are suggested to from an antioxidant barrier surrounding the nucleus and
the chloroplast and protecting these structures from ROS attacks (Boussiba 2000; Hagen et al. 1993b). Possible
antioxidative effect of Ast in vivo have been discussed (Kobayashi 2000; Kobayashi et al. 1997a), but it is unlikely
that Ast exerts direct antioxidative effect by elimination of the ROS in the thylakoid membranes because this
pigment is accumulated in cytoplasmic LDs, which are distant from the sites of ROS generation in the chloroplast.
On the other hand, Ast was found to be capable of binding to the photosynthetic pigment-protein complexes and to
accumulate within the thylakoid membranes in transgenic plants (Röding et al. 2015). Currently, it seems more
likely that the protection of the cell at the initial phase of the stress inducing Ast accumulation is performed by the
“classic” antioxidative enzymes superoxide dismutase, catalase, and peroxidase ((Wang et al. 2004), see also Fig. 2).
These enzymes are generally undergo transient up-regulation before the onset of the gross accumulation of Ast,
usually within the first two days of the stress exposure. There are also some clues from the recent metabolomic
studies (Su et al. 2014) pointing to the possible involvement of the molecules with antioxidative function such as
thioredoxin(s) and glutathione in the stress response of H. pluvialis. The putative antioxidative role of Ast is
expected to be significant in the LD where Ast can protect the lipids containing unsaturated FA prone to
peroxidation.
Thirdly, biosynthesis of Ast consumes, as the substrate of β-carotene hydroxylase and ketolase (see below),
O
2
potentially dangerous for the cell under stress. The relative decline in steady-state O
2
concentration in the cell
during vigorous Ast accumulation is estimated to be above 10% of its stationary concentration which may be
significant under adverse conditions (Li et al. 2008a). Molecular oxygen is also reduced to H
2
O in the course of
5
carotenoid biosynthesis by the enzyme plastid terminal oxidase (PTOX), the co-factor of phytoene and ζ-carotene
desaturases (Bennoun 2001; Li et al. 2010) (Fig. 1); for a comprehensive account of PTOX roles in phototrophic
cell, see the recent review by Nawrocki et al. (2015). It was also shown that the induction of Ast biosynthesis is
coordinated with the up-regulation of the expression of the genes of antioxidative defense (Gwak et al. 2014; Kim et
al. 2011).
Finally, the biosyntheses of Ast and FA (see the section “Coupling of astaxanthin and lipid biosyntheses
below) provide a potent sink for the photosynthates that cannot be utilized for cell growth and division under the
stress. This sink further mitigates the risk of damage by ROS increasingly accumulated when the electron carriers in
the chloroplast electron transport chain are over-reduced (Han et al. 2012).
Massive astaxanthin accumulation and photosynthesis
It is conceivable that biosynthesis of Ast and lipids of LD as well as maintenance of the cell homeostasis under the
stressful conditions should generate a considerable demand of energy and building blocks for the carotenoid and
lipid biosynthesis. A significant level of metabolic activity during the initial phase of carotenogensis is thought to be
necessary for a successful transformation of the “green” cell to Ast-rich “red” cells (Gwak et al. 2014). On the other
hand, previously obtained physiological evidence and the recent analysis of the gene expression pattern showed that
the formation of the “red” cells is accompanied by down-regulation of photosynthesis and enzymes responsible for
biosynthesis of chlorophyll, LHC (Gu et al. 2014; Gwak et al. 2014) and enzymes of carbon fixation (Kim et al.
2011). This is consistent with the observed at the ultrastructural level reduction of chloroplast, decomposition of the
grana and lamellae system (Gu et al. 2013; Peled et al. 2012), probably to reduce the production of ROS in the cell
(Wang et al. 2004).
However, recent evidence indicates that a considerable part of photosynthetic activity is retained during the
onset and subsequent progress of the carotenogenic response in spite on the profound decomposition of thylakoids.
Thus, Gu et al. (2013) argued that even fragmented thylakoid membranes are able to maintain a moderate level of
photosynthetic activity in H. pluvialis cells. This seems to be possible since the initial phase of Ast accumulation is
accompanied only by a transient downregulation of D1 protein of PSII although without a sizeable decline in the
rate of photosynthetic oxygen evolution (Wang et al. 2003) and a certain amount of chlorophyll and accessory
pigments is also retained even at the most advanced stages of Ast accumulation. Hagen et al. (1993a) also detected
an increase in the transthylakoid proton gradient along with oscillations in non-photochemical fluorescence
quenching and attributed this to active CO
2
fixation in the course of “red” cell formation.
Basing on the measurements of respiration rates and metabolic activity of the “red” H. pluvialis cells, it was
suggested that insufficient energy supply by photosynthesis under stressful conditions could be compensated by
elevated respiration and glycolysis processes (Hagen et al. 1993b; Recht et al. 2014; Recht et al. 2012). This
suggestion is compatible with the transient up-regulation of mitochondrial respiratory proteins after the onset of Ast-
inducing stress (Wang et al. 2004). The increased demand in ATP could also be satisfied by increased
phosphorylation as a result of up-regulation of cyclic electron transport over PS I and overall increase in the PS I to
PS II activity ratio (Hagen et al. 1993a).
Key steps of asthaxanthin biosynthesis
6
The biochemistry and enzymology of Ast biosynthesis as well as its genetic control are relatively well studied and
extensively reviewed elsewhere (Cunningham Jr and Gantt 1998; Han et al. 2013b; Lemoine and Schoefs 2010;
Nisar et al. 2015; Takaichi 2011) so the key steps of Ast biosynthesis are just briefly recapitulated here. More
attention is paid to the recently discovered metabolic constrains and regulatory events accompanying the induction
and progress of Ast accumulation in microalgae (see the next section).
As in higher plants, the precursor of Car in Chlorophyta is isopentenyl pyrophosphate (IPP, C
5
) originating
from glycerophosphate-pyruvate (non-mevalonate or 1-deoxy-D-xylulose-5-phosphate, DOXP) pathway whereas in
Euglenophyceae it is formed via mevalonate pathway (Han et al. 2013b; Lichtenthaler 1999; Zhao et al. 2013).
Direct evidence on the gene level were recently obtained for the involvement of the DOXP pathway in IPP
biosynthesis in H. pluvialis (Gwak et al. 2014). The enzyme IPP isomerase (IPI, encoded in H. pluvialis by
oxidative stress-induced Ipi1 or translation-regulated Ipi2 gene (Sun et al. 1998)) converts IPP to dimethylallyl
pyrophosphate (DMAPP). Successive attachment of three IPP molecules to a DMAPP molecule by geranylgeranyl
pyrophosphate (GGPP, C
20
) synthase yields the molecule of GGPP. Two GGPP molecules undergo head-to-tail
condensation to form phytoene in the reaction catalyzed by phytoene synthase (PSY, encoded by the gene Psy)
(Cunningham Jr and Gantt 1998).
Phytoene molecule is the precursor of all carotenoids. In four sequential desaturation reactions catalyzed by
phytoene desaturase (PDS, Pds1) and ζ-carotene desaturase (ZDS, Zds1), it is converted sequentially to phytofluene,
ζ-carotene, neurosporene, and lycopene (5, 7, 9, and 11 conjugated double bonds, respectively) (Cunningham Jr and
Gantt 1998). PTOX is a plastoquinol oxidase serving as a co-factor of the desaturases and the key oxidase in
chlororespiration (Nawrocki et al. 2015). Wang et al. (2009) cloned and sequenced two PTOX cDNAs from H.
pluvialis, designated as ptox1 and ptox2 participating in Ast synthesis and playing a critical protective role against
stress by reducing O
2
to H
2
O (Bennoun 2001; Li et al. 2010). Comparative analysis of the transcriptional expression
of ptox1, ptox2 and pds indicated that the up-regulation of PTOX1 but not PTOX2 is correlated with Ast synthesis
(Wang et al. 2009). The membrane-bound enzyme lycopene β-cyclase (LCYB, LcyB) sequentially converts the
linear molecule lycopene to β-carotene possessing two β-ionone rings (Cunningham Jr and Gantt 1998).
All steps mentioned above take place in the chloroplast. It was shown that β-carotene is the Ast precursor
which is exported from the cytoplasm (Grünewald and Hagen 2001) and the remaining steps of Ast formation (see
the section “Lipid droplets: factories and subcellular depots of astaxanthin”) occur in the LD subcompartment. The
mechanism(s) of β-carotene transport to the LD remains so far elusive: though the simultaneous presence of β-
carotene and Ast in H. pluvialis LD was confirmed by Raman microspectrometry (Collins et al. 2011), electron-
microscopic examination did not reveal any structures related to the transport of β-carotene (Grünewald and Hagen
2001).
The conversion of β-carotene to Ast require introduction of two hydroxyl groups (in the positions 3 and 3′)
and two keto-groups (in the positions 4 and 4′). The former reaction is catalyzed by 3,3′-hydroxylase CRTR
(encoded by ChyB), and the latter by 4,4′-ketolase CRTO (CrtO) or BKT represented by several forms, Bkt1Bkt3,
in different H. pluvialis strains (Grunewald et al. 2001). Under stress, multiple BKT genes are up-regulated and upon
reaching a certain threshold level of BKT transcripts, H. pluvialis begins to massively synthesize Ast (Huang et al.
2006).
A large body of evidence reviewed by Lemoine and Schoefs (Lemoine and Schoefs 2010) suggests that the
addition of ketogroups occurs before the hydroxylation and the formation of Ast from β-carotene in H. pluvialis
proceeds most likely (and predominantly) via echinenone (one keto-group), canthaxanthin (two keto-groups), and
adonirubin (two keto- and one hydroxygroup). At the same time, this is not necessarily the case in all Ast-
7
accumulating microalgae. Indeed, a BKT from H. pluvialis (BKT3) expressed in E. coli exhibited the lowest
efficiency of the conversion of zeaxanthin to Ast whereas analogous enzymes from C. reinhardtii (CrBKT) and
Chlorella zofingiensis (CzBKT) were much more efficient (Zhong et al. 2011).
Coupling of lipid and astaxanthin biosyntheses in the course of carotenogenesis: source of
value-added carotenoids and biofuel precursors
As was earlier established by Zhekisheva et al. (2002), accumulation of Ast is tightly related with the biosynthesis of
FA such as oleic acid associated predominantly with triacylglycerols. This class of neutral lipids is the major
constituent of cytoplasmic LD forming the intracellular depot for Ast (see the section «Lipid droplets: factories of
and subcellular depots for astaxanthin» below). Fatty acids are also consumed for esterification of polar hydroxyl
groups of Ast prior its deposition within the hydrophobic environment of the LD. Accordingly, inhibition of the
lipid biosynthesis abolished the accumulation of Ast whereas blocking Ast biosynthesis did not prevent the
accumulation of neutral lipids and formation of LD (Zhekisheva et al. 2005). The results obtained by Chen et al.
(2015) disproved possible coordination of lipid and Ast biosynthesis at the transcriptional level and confirmed that
this interaction was feedback related at the metabolite level. The in vivo and in vitro experiments of these authors
indicated that Ast esterification by a specific diacylglycerol acyltransferase is the process driving the formation and
accumulation of Ast.
The bulk of Ast in H. pluvialis is in the form of mono- and diesters of palmitic (16:0), oleic (18:1) or
linoleic (18:2) FA. The composition of Ast esters in H. pluvialis red cysts is comprised by ca. 70% monoesters, 25%
diesters and 5% of the free ketocarotenoid (Johnson and Schroeder 1995; Zhekisheva et al. 2002). Fatty acid content
in the “red” cell lipids of H. pluvialis can be as high as 3060% of DW with > 80% unsaturated FA (Goncalves et al.
2013). As argued by Damiani et al. (2010), the capacity of H. pluvialis to accumulate high (up to 32.99 % of the cell
dry weight) amounts of the neutral lipids incorporating predominantly moderately unsaturated FA from the families
C16 and C18 in response to the stresses makes it a potential oil-enriched feedstock for biodiesel production. Indeed,
there were attempts to estimate the suitability of H. pluvialis biomass for the conversion to biodiesel (Damiani et al.
2010). However, nowadays the use of H. pluvialis biomass for the extraction of Ast is much more economically
viable than the production of biodiesel. Still, the bulk lipid and biomass waste remaining after extraction of Ast can
be valorized via conversion to biodiesel and/or other kinds of biofuels. In this case, the Ast remaining in the lipid
extract or biomass could serve as a natural antioxidant protecting the biofuel against oxidative degradation making it
more suitable for long-term storage.
A model was recently developed integrating the stress-responses of Ast biosynthesis, carbohydrate and
lipid metabolism in H. pluvialis (Recht et al. 2014; Recht et al. 2012). The up-regulation of the Ast biosynthesis
takes place on the background of the transient induction of carbohydrate accumulation (Recht et al. 2014).
Metaboliс profiling showed that as long as Chl content is relatively high (> 12 mg L
−1
which is the case in the
“brown” cells), the cell invests its carbon and energy to the pool of free sugars and starch. Further exposure to stress
causes a transition to degradation of previously accumulated carbohydrates (Recht et al. 2012) and synthesis of fatty
acids (Recht et al. 2014), as in different chlorophyte species (Goncalves et al. 2013; Gorelova et al. 2014).
The advanced stages of stress-induced carotenogenesis when the rate of Ast biosynthesis slows down
(Recht et al. 2014) might be accompanied by a shutdown of central metabolism (judging from ATP and other NTPs
as well as ribulose bisphosphate levels) except the pathways responsible for FA biosynthesis de novo (as reflected
8
by acetyl-CoA, dihydroxyacetone phosphate, and 3-phosphoglycerate levels; see also Fig. 1). The conspicuous
metabolic changes during the induction Ast accumulation also involve the increase in the pool of acetyl-CoA, a key
precursor of FA synthesis, and the stearic and palmitic acids (Su et al. 2014) which are the major FA esterifying Ast
(Zhekisheva et al. 2002). The up-regulation of other genes related with FA synthesis such as alkane 1-
monooxygenase, alcohol dehydrogenase, triacylglycerol lipase took place under stress conditions including both
nutrient starvation and high irradiance (Kim et al. 2011).
The recent findings by Recht et al. (2014) are also indicative of the relationship between the
carotenogenesis and the increased activity of the tricarboxylic acid (TCA) cycle. It was suggested that TCA cycle
plays a key role in FA biosynthesis under stressful conditions conductive for the carotenogenesis furnishing excess
malate, which could support FA biosynthesis after import to the chloroplast (Fig. 1b). This is compatible with the
increase in respiration-related transcripts (i.e. glycolysis, TCA cycle, electron transport, phosphorylation) during Ast
accumulation in Haematococcus (Kim et al. 2011). Using inhibitor analysis, Recht et al. (2014) confirmed the
involvement of malic enzyme in the induction of fatty acid biosynthesis accompanying the carotenogenic stress
response and proposed the existence of a significant flux of malate to the chloroplast with its subsequent
involvement in de novo FA biosynthesis. In contrast to Chlamydomonas reinhardtii (Shtaida et al. 2014), it is
unlikely that in the stressed H. pluvialis a high amount of carbon flows from pyruvate to Acetyl-CoA, and
continuous carbon fixation (see the section “Massive astaxanthin accumulation and photosynthesis”) suggest that
pyruvate flows into other pathways e.g. the non-mevalonate pathway for the biosynthesis of Car.
Lipid droplets: factories of and subcellular depots for astaxanthin
It is conceivable that secondary Car including Ast cannot accumulate in appreciable amounts within the thylakoid
membranes (which composition is under strict genetic control) or, in the free form, within the hydrophilic
environment of the cytoplasm. Indeed, the presence of Ast in the chloroplast membranes of the transgenic tobacco
lead to destabilization of its lipid phase and PS II supercomplexes (Röding et al. 2015). The massive accumulation
of Ast under stress turns feasible in the presence of dedicated subcellular structurescytoplasmic LD forming a
potent sink for Ast, the end product of the corresponding pathway, avoiding the feedback inhibition of the Car
biosynthesis. It was conclusively demonstrated that formation of the LD subcompartment in the cell is among key
factors driving (Zhekisheva et al. 2002) and limiting (Zhekisheva et al. 2005) accumulation of Ast in the cells of
carotenogenic microalgae (Lemoine and Schoefs 2010). This is one of the reasons making massive accumulation of
secondary Car more feasible from the standpoint of biotechnology in comparison with overproduction of primary
Car (e.g. lutein and fucoxanthin). The latter is more problematic since it will require modification of gene expression
or enzyme activity, most likely combined with the creation of storage structures outside of the photosystems
(Mulders et al. 2014b).
The exact mechanism of LD formation is so far not known though it is commonly accepted that the
globules are formed from vesicles budding from the endoplasmic reticulum (Guo et al. 2009; Murphy 2001b). This
mechanism is more similar to that common for a different cell types including bacterial, higher plant and animal
cells whereas the plastidial lipid globules of Dunaliella salina accumulating β-carotene are more closely related to
stigma-like structures (Davidi et al. 2014).
The bulk (> 90% of total FA content) of the inner LD matrix is formed by neutral lipids, mainly
triacylglycerols (TAG); polar phospholipids (PC), sulfolipids (SQDG), and glycolipids (mainly MGDG) as well as
9
betain lipids (DGTS) constituting monolayer LD membrane and specialized amphifilic proteins (Murphy 2001a;
Peled et al. 2011). The major FA in the lipids of LD are palmitic (16:0), oleic (18:1), and linoleic (18:2) acids (Peled
et al. 2011; Zhekisheva et al. 2002).
The major LD-forming protein in H. pluvialis (Haematococcus oil globule protein, HOGP) was 275-amino
acid long 33-kDa protein partially homologous to Ch. reinhardtii oil globule protein. This protein was hardly
detectable in vegetative cells but increased more than 100 fold within 12 h of nitrogen deprivation and/or high light
stress exposure (Peled et al. 2011). The major LD-associated proteins of green microalgae are thought to be encoded
by a novel gene family specific to Chlorophyta. The globule-associated protein biosynthesis might be regulated at
the translational or post-translational level to sustain the biogenesis and enormous accumulation of the LD (Gwak et
al. 2014).
As noted above, relatively polar Ast molecules are esterified by fatty acids before deposition in the LD. As
reported by Chen et al. (2015), Ast esterification occurs in the endoplasmic reticulum. Thus, in H. pluvialis and Ast-
accumulating representatives of the genus Chlorella during the final steps of cyst formation, more than 95% of Ast
are converted to fatty acid esters (Sussela and Toppo 2006). There are controversial reports on the Car composition
of LD in H. pluvialis: biochemical methods reveal only Ast in the LD (Peled et al. 2011) whereas Raman
microspectrometry is evident of the simultaneous presence of β-carotene and Ast in H. pluvialis LD (Collins et al.
2011). As shown by nonlinear optical microscopy imaging, the Ast accumulated within the LD of H. pluvialis is
characterized by highly ordered isotropic packaging; this is in contrast to the highly anisotropic organization of Ast
in synthetic aggregates (Tokarz et al. 2014). This might be an additional indication of the involvement of the
enzymes associated with the LD membranes in the biosynthesis of the Ast molecules.
Recent studies emphasized another important role of LD as a structure where the final steps of Ast
biosynthesis takes place. Therefore, it is possible to think that blocking the stress-induced biosynthesis of lipids and
LD formation abolishes the accumulation of Ast not only due to the lack of the sink for Ast but due to disturbance of
the oxygenation of β-carotene as well. This is not typical for either higher plants or microalgae: thus, the β-carotene
containing globules of Dunaliella salina are situated in the chloroplast and do not participate in the carotenoid
biosynthesis (Davidi et al. 2015; Davidi et al. 2014). Interestingly, the literature available at the time of this writing
lacked reports on the detectable accumulation of Ast precursors in the LD compartment.
The evidence of participation of the LD in Ast biosynthesis stems from inhibitory and
immunocytochemical analyses showing that in H. pluvialis, CRTO is present not only in chloroplasts but also in the
LD (Grunewald et al. 2001). As also noted by Grünewald et al. (2001), CRTO molecules are observed not only in
the LD periphery but also inside these structures, which is in agreement with hydrophobic properties of this enzyme.
In fact, CRTO is active in the hydrophobic surrounding, such as TAG matrix of the LD. It is likely that in such
medium CRTO is protected from the protease attack, which may explain continued enzyme activation on the
background of a decrease in its mRNA content. It is worth mentioning that, in spite of CRTO presence inside LD, its
molecules are active only on the OS surface. Apparently, this is explaned by the requirement of co-factors coming
from other compartments, such as the endoplasmatic reticulum and Golgi apparatus, this suggestion is supported by
co-localization of OS and these structures (Grunewald et al. 2001).
Induction and regulation of massive astaxanthin accumulation
10
Generally, accumulation of secondary Car including Ast in microalgal cells takes place under adverse conditions
slowing down the cell division and photosynthesis e.g. under excessive irradiance, nutrient deficiency, extreme
temperatures, salinity and their combinations (Boussiba 2000; Solovchenko 2013). Different stresses inducing
accumulation of Ast affect the expression of approximately the same subset of the genes controlling Car
biosynthesis. Thus, the high light and salinity stresses simultaneously up-regulate the transcription of many genes
starting from the formation of IPP and including β-carotene hydroxylase (see the section “Key steps of astaxanthin
biosynthesis”) (Gao et al. 2014; Kim et al. 2011; Mulders et al. 2014b). The level of CrtR-b transcripts and Ast
accumulation were linearly related the H. pluvialis wild type and the Ast-hyper-accumulating mutant (MT 2877),
suggesting a transcriptional control of CrtR-b over Ast biosynthesis (Li et al. 2008a; Li et al. 2010). On the other
hand, the amount of BKT protein increases in parallel with accumulation of the corresponding bkt transcript only
during the early steps of Ast synthesis whereas a further enzyme accumulation is not accompanied by a proportional
increase in the mRNA amount. This may indicate also the involvement of not only transcriptional, but also
(post)translational mechanisms in the regulation of this enzyme activity (Grunewald et al. 2000).
Li et al. (2008b) demonstrated that most of the Car synthesis genes are up-regulated at the transcriptional
level in response to high light, excess ferrous sulfate and excess sodium acetate. Interestingly, the combination stress
(e.g. high light + salt stress + iron stress) induced lower initial levels of the gene transcripts in comparison with the
individual stresses which, after a certain lag, exceeded the levels of the individual stresses (Li et al. 2008b). It is
important to note that carotenogenic response serves the long-term protection and survival of the cell under extended
exposure to the stress, whereas the ‘classical’ enzymatic defense systems mainly serve as short-team defense
mechanisms in H. pluvialis ((Wang et al. 2004), see also Fig. 2). This might also explain a delayed expression of
the carotenogenesis genes with greater expression levels occurred in the cultures treated with the multiple stressors
(Li et al. 2008b).
The authors hypothesized that H. pluvialis, like other microalgae possess both ‘generic’ (independent on
the nature of the stressor) and stressor-specific defense mechanisms. In this case, the carotenogenic response is
largely induced by high light, whereas the other defense reactions may be triggered by salinity or excess iron ion.
Should this be the case, the combination stress would activate, in a coordinated manner, different protection
mechanisms with different efficiency of the induction of carotenogenesis. On the other hand, there are reports
suggesting that most of the stresses are essentially interchangeable regarding the induction of Ast accumulation
(Kobayashi et al. 1993; Kobayashi et al. 1997b).
The stresses are thought to be sensed by the photosynthetic cells via a number of putative sensors such as
plastoquinone pool reduction state, membrane fluidity, stationary concentration of ROS and/or some metabolic
‘hub’ enzymes (Huner et al. 2012). Thus, a common effect of the environmental stresses is the increase of
stationary concentration of ROS in the cell (Foyer and Shigeoka 2011; Sirikhachornkit and Niyogi 2010). On the
other hand, there is ample evidence suggesting the involvement of ROS, probably as secondary messengers, in the
induction of Ast accumulation (Yong and Lee 1991). The role of ROS as the messengers responsible for the
crosstalk between different stimuli is evidenced by treatment of the microalgal cells with ROS generators e.g. H
2
O
2
which induces accumulation of Ast even in the absence of the other stimuli mimicking the action of environmental
stressors; on the contrary, ROS scavengers suppress massive accumulation of Ast (Kobayashi et al. 1997a).
Supplementation of the microalgal culture with organic carbon sources such as acetate or sugars causing
feedback inhibition of photosynthetic carbon fixation (but not for primary photochemistry) is also conductive for
Ast accumulation (Kobayashi 2000; Kobayashi et al. 1993). These findings are compatible with suggestion that
biosynthesis of Ast is regulated by plastoquinone pool as a redox sensor (Steinbrenner and Linden 2003) which is
11
mostly reduced under the stressful conditions when the light absorption exceeds the energy utilization capacity of
dark reactions of photosynthesis (Lemoine and Schoefs 2010). In addition, the over-reduced electron carries in the
chloroplast electron transport chain might further augment the ROS pool in the stressed algal cells.
An interesting research avenue is represented by studies of phytohormone stimulation of Ast accumulation
by microalgal cells (Gao et al. 2013a; Gao et al. 2013b). In the H. pluvialis cells, gibberllin A3 (GA3) treatment
differentially up-regulated the genes Ipi-1, Ipi-2, Psy, Pds, and Bkt2 in a concentration-dependent manner. Thus,
GA3 in concentration 20 mg L
1
had a greater effect on the expression of Bkt2 whereas 40 mg L
1
of GA3 lead to a
stronger up-regulation of Ipi-2, Psy, and Bkt2. The expression of Lyc, CrtR-B and CrtO was not affected by GA3
(Gao et al. 2013a). Another plant hormone, epibrassinolide (EBR) increased, in the concentrations of 25 or 50 mg L
1
, Ast productivity via up-regulation of the eight genes of the carotenoid biosynthesis pathway at the transcriptional
(Pds, Lyc, CrtR-B, Bkt, and CrtO), post-transcriptional (Ipi-1 and Psy) or both levels (Ipi-2) (Gao et al. 2013b). The
comparative studies of the phytohormone effects in the microalgae and higher plants are expected to reveal new
information about the regulation of the carotenogenesis. Exogenous application of GA3 and EBR is also considered
as a promising way of enhancement of Ast productivity of H. pluvialis mass cultures.
Is there a way for increasing microalgal astaxanthin productivity?
As reviewed by Mulders et al. (2014b), microalgae have a number of advantages as a source of natural compounds,
including Ast, since these organisms i) selectively accumulate the pigment of interest (up to ca. 95% of the total
carotenoids) in the amounts by far exceeding those of e.g. higher plants; ii) grow rapidly; iii) do not compete with
crops for arable land. Currently, H. pluvialis is the most biotechnologically significant microalgal producer of Ast
despite of its intrinsic shortcomings such as slow growth rate, low biomass yield, high risk of contamination at the
green stage and a high light requirement for the induction of Ast biosynthesis. Another highly promising
candidate species is represented by C. zofingiensis (Mulders et al. 2014a; Mulders et al. 2015). Its advantages
include faster growth rate in a phototrophic, heterotrophic or mixotrophic cultures, also at ultrahigh cell densities
(Han et al. 2012; Liu et al. 2014).
However, the cost efficiency of current microalgal biotechnologies for production of Ast from microalgae
is severely limited by productivity of known strains (Lorenz and Cysewski 2000). Indeed, only a limited number of
microalgal species (apart from H. pluvialis), exclusively from Chlorophyta, are capable of synthesizing Ast. A
comprehensive list compiled by Han et al. (for more detail, see (Han et al. 2013a) and references therein) includes
Botryococcus braunii (0.01% DW), Chlamydocapsa spp. (0.04% DW), Chlamydomonas nivalis, Chlorococcum sp.
(0.7% DW), Chloromonas nivalis (0.004% DW), Eremosphera viridis, Neochloris wimmeri (1.9% DW),
Protosiphon botryoides (1.4% DW), Scenedesmus sp. (0.3% DW), Scotiellopsis oocystiformis (1.1% DW), and
Trachelomonas volvocina. More recent addition to this list is comprised by Bracteacoccus minor (Minyuk et al.
2014) and Euglena sanguinea (G. Minyuk, personal communication). Even more limited number of the chlorophyte
species are able to overproduce Ast i.e. accumulate this pigment in biotechnologically significant amounts (45%
DW) under the stressful conditions (Liu et al. 2014).
One may expect a certain increase in Ast productivity from optimization of cultivation conditions.
Considering the two-stage cultivation approach commonly applied for production of Ast from H. pluvialis (for an
account of the biotechnology of Car from microalgae, see (Del Campo et al. 2007; Han et al. 2013b; Leu and
Boussiba 2014; Lorenz and Cysewski 2000; Solovchenko and Chekanov 2014)), this will presume (i) increasing the
12
biomass productivity at the green stage and (ii) further optimization of the cultivation conditions. However, this
avenue of increasing algal productivity is highly explored and exploited already (Mulders et al. 2014b) so it is
unlikely to produce a revolutionary increase in Ast productivity.
A promising way of achieving Ast accumulation above the 5% DW threshold (which is a prerequisite for
favorable competition of the natural Ast from microalgae with the synthetic pigment (Del Campo et al. 2007)) is
offered by microalgal strain engineering. One of the approaches is strain manipulation at the regulatory level aiming
at the removal of possible bottlenecks of Ast biosynthesis. Generally, to increase the metabolic flux towards the
biosynthesis of the pigment of interest one needs to over-express the enzyme(s) that exert control over the flux
towards the desired pigment (or replace/augment the rate-limiting enzymes by more efficient analogs from different
organisms), without much disturbance to the rest of the metabolic network of the cell (Farré et al. 2014; Farré et al.
2015). However, one should beware e.g. of competition between secondary and primary carotenoid biosyntheses
which will most like result in growth inhibition. Possible targets of this approach include the enzymes catalyzing
rate limiting steps in the biosynthesis of Ast e.g. phytoene desaturation and lycopene cyclization (Lemoine and
Schoefs 2010) as well as ketolation of β-carotene (Han et al. 2013a). The strains with increased Ast accumulation
capacity are characterized by the early up-regulation of the genes controlling the rate-limiting steps of the carotenoid
biosynthesis e.g. psy, lyc, bkt2, crtR-B and crtO (Gao et al. 2014). This finding may provide a hint for selection of
the target genes for up-regulation. On the other hand, the increased transcript level of these genes is not correlated to
the different Ast accumulation levels so the expression patterns of these genes are likely strain-specific (Gao et al.
2014).
Still, metabolic pathways in microalgae are complex, with numerous regulatory inputs and interplay with
other pathways so it is difficult to find the best manipulation target(s) and predict the outcome with confidence
(Farré et al. 2014; Farré et al. 2015). In the case of Ast this approach is complicated at least by (i) distribution of the
enzymes of Ast biosynthesis between compartments (chloroplast and LD), (ii) a potential bottleneck associated with
the export of β-carotene from the chloroplast to the LD which mechanism remains so far elusive. Remarkably, a
sufficiently developed transformation method and genetic toolbox were developed recently (Sharon-Gojman et al.
2015). Still, advanced knowledge-based metabolic engineering based on large “omics” dataset mining opens the
way for a precise identification of relevant genes and regulatory mechanisms yielding models that predict the most
suitable manipulation points. More sophisticated strategies based on finetuned transgene expression,
thermodynamic and kinetic models will be then necessary to balance the metabolic fluxes in the entire Ast
biosynthesis pathway (for a comprehensive account of the metabolic engineering strategy, see e.g. (Farré et al.
2015)).
Concluding remarks
Recent insights unveiled the amazing picture of the carotenogenic response as a sophisticated, precisely regulated
and tightly integrated into the metabolic network mechanism for coping with diverse stresses. As a result, a number
of paradigms related with the role of Ast in the stress tolerance of phototrophs were gradually changed. It is now
clear that Ast biosynthesis is not mere an isolated branch of secondary carotenoid biosynthesis. Its integration with
central metabolism (especially with carbohydrate and fatty acid biosyntheses) is much more complex (Gwak et al.
2014; Recht et al. 2014) than it was suggested earlier. Another striking finding originated from the comparative
transcriptomic and lipidomic analysis is that the Ast-rich resting cells of H. pluvialis are probably not quiescent at
13
all, but instead are more metabolically active than the motile vegetative “green” cells (Gwak et al. 2014). Then, the
cytoplasmic lipid droplets (LD) serving as the depot for Ast synthesized during “green” to “red” cell transition
appear to be much more than just a intracellular reservoir for the lipids and carotenoids. On the contrary, LD play a
key role in the biosynthesis of Ast (Grunewald et al. 2001; Lemoine and Schoefs 2010; Peled et al. 2011) and
participate in active protection of the microalgal cell (Peled et al. 2012).
Further significant increase in Ast content for currently known native strains by means of cultivation
condition adjustment does not seem to be realistic now. At the same time, the natural algal diversity, which is so far
underexplored, may well surprise us with yet unknown strains with high carotenogenic capacity. At the same time
the limitations of native organisms such as the limitation imposed by the capacity of the intracellular Ast depots may
be overcome, with time, using engineering approaches. In particular, expansion of the LD subcompartment would
enhance this sink for Ast eventually improve its accumulation. Probably, this goal can be achieved via engineering
for the enhanced lipid production using one of the techniques recently developed for the improvement of biofuel
producing strains (Rosenberg et al. 2008; Trentacoste et al. 2013).
It is generally (and rightfully) believed now that the direct use of Ast-rich microalgal biomass for
production of biofuel is not economically viable. On the other hand, there are largely unexplored possibilities of
turning the waste generated from the processing of Ast-rich biomass and purification of Ast for human consumption
to valuable bioproducts including different kinds of biofuels. In particular, the neutral lipids co-accumulated with
Ast turned to be highly suitable for conversion to biodiesel (Damiani et al. 2010).
Finally, despite the significant progress outlined above, our understanding of the biology of Ast in the
microalgal cell is still far from complete. In particular, much more work is needed to decipher the machinery of the
Ast transport between the chloroplast and LD compartments. Another enigmatic process is that of Ast catabolism
presumably taking place in the cells reverting from the “red” to the “green” stage. Nevertheless, should the
investigation of the carotenogenesis and the related processes in the cell remain as vigorous as they are now, there
would be a good chance to find the missing answers in the nearest future.
Acknowledgements Helpful notes of Mr. Konstantin A. Chekanov are greatly appreciated.
Funding This study was funded by Russian Scientific Fund (project #14-50-00029).
Conflict of Interest The author declares that he has no conflict of interest.
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Figure legends
Fig. 1. The schematic view of major changes in photosynthetic electron flow and carbon partitioning patterns of (a)
the “green” cells of H. pluvialis in the course of the stress-induced accumulation of astaxanthin (b) with an emphasis
on lipid and carotenoid synthesis. In the “green” cells, linear electron flow in the electron transport chain of
chloroplast dominates and the fixed carbon mainly partitioned between structural components of the cell and
photosynthetic pigments. The onset of carotenogenesis is accompanied by (1) reduction of photosynthetic apparatus
and (2) increase of the cyclic electron flow contribution. The photosynthetically fixed carbon, as well as metabolites
re-partitioned from other metabolic pathways (such as (3) starch turnover and (4) tricarbonic acid cycle) are
converted mainly to astaxanthin (5) and (6) fatty acids consumed for the assembly of neutral lipids and esterification
of the astaxanthin molecules. Compiled from (Gu et al. 2014; Gwak et al. 2014; Recht et al. 2012; Su et al. 2014).
Fig. 2. A hypothetic scenario of the physiological changes accompanying the induction of astaxanthin accumulation
in the stressed cells of H. pluvialis. At the initial stages of the astaxanthin accumulation, the cell retains a significant
amount of photosynthetic pigments and photosynthetic activity driving accumulation of starch. At this stage, the up-
regulated antioxidative enzymes protect the cell. Later, the starch synthesis is followed by its degradation, a rise of
respiration rate and induction of fatty acid and astaxanthin biosynthesis resulting in the appearance of red lipid
droplets in the cell. Decline in photosynthetic pigments proceeds (although the specific photosynthesis rate might be
preserved), the antioxidative enzyme activity reverts to the basal level.
a)
b)
cell
division
rate
chlorophyll+
photsynthetic
carotenoids
astaxanthin
absolute
photsynthesis
antioxidative
enzymes
neutrallipids
starch
GREEN
(motilevegetative
cells)
BROWN
(immotilecellswith
intermediateCar/Chl
RED
(restingcells,
haematocysts)
Stressexposure
respiration
... AST synthesis in H. pluvialis occurs in response to high salinity, stress due to high light intensity, nitrogen or phosphorus deficits, and the presence of salicylic acid and ethanol [25]. Moreover, AST synthesis in H. pluvialis is directly correlated in space and time with the deposition of cellular reserves in lipid droplets under conditions of cellular stress [26]. Meanwhile, the key to AST biosynthesis in H. pluvialis is through the nonmevalonate (MEP) pathway which belongs to the isopentenyl pyrophosphate (IPP) pathway [9]. ...
... In the world market scale, the need for AST is mostly met through the synthesis of AST compared to natural pigment sources, but attention to alternative sources of AST from microorganisms in recent years has increased, especially in the types of H. pluvialis and P. rhodozyma which are useful for the production of AST on an industrial scale [13]. H. pluvialis has a carotenoid response that makes it able to survive in an unfavorable environment, making it a source of AST that is commonly used on an industrial scale [26]. However, the production of AST from H. pluvialis is quite expensive due to the fact that it requires a high-capacity photobioreactor growth medium, whereas its growth was slow when using traditional media exposed to H. pluvialis, and it would produce AST which is protected by thick-walled immobile hematocysts [25,30]. ...
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Recent interest in carotenoids has increased due to their antioxidant and production performance. Astaxanthin (AST) is a xanthophyll carotenoid abundantly distributed in microalgae, which is described as a highly potent antioxidant. Therefore, recent studies have tended to investigate the role of antioxidants in improving metabolic processes and physiological functioning of the body. It is now evident that AST could significantly reduce free radicals and oxidative stress and help to maintain a healthy state. Moreover, AST also could improve the performance of broiler chicken by increasing the daily feed intake, followed by improvement in the food conversion rate.
... In the absence of additional photosynthetic growth, chlorophyll pigments can be degraded and reallocated to support the metabolic activity of algal cells exposed to adverse culture conditions. Solovchenko [28] reported that during the photosynthetic cultures of H. pluvialis, Chlamydomonas reinhardtii, and C. zofingiensis, down-regulation of chlorophyll synthesis was associated with subsequent upregulation of astaxanthin and lipid synthesis. Contrary to the results of the cell growth promotion (Figure 2a), the astaxanthin contents of the rac-GR24-treated cells (60.1-65.0 ...
... This indicates that rac-GR24 does not promote astaxanthin biosynthesis in H. pluvialis cells under the present photoautotrophic culture conditions. Cell growth rate and astaxanthin accumulation in H. pluvialis generally show opposite trends under photosynthetic conditions [2,28]. However, it should be noted that at the highest dosage of 8 µM rac-GR24, the volumetric astaxanthin production (26.1 ± 1.7 mg/L) considerably improved by 21% compared to the control (21.6 ± 1.5 mg/L) due to the improvement of cell number density. ...
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Improving the production rate of high-value nutraceutical compounds, such as astaxanthin and polyunsaturated fatty acids (PUFAs), is important for the commercialization of Haematococcus pluvialis biorefineries. Here, the effects of a phytohormone, strigolactone analog rac-GR24, on cell growth and astaxanthin and fatty acid biosynthesis in H. pluvialis were investigated. Four concentrations (2, 4, 6, and 8 µM) of rac-GR24 were initially added during 30 days of photoautotrophic cultivation. The addition of rac-GR24 improved cell number density and chlorophyll concentration in H. pluvialis cultures compared to the control; the optimal concentration was 8 µM. Despite a slightly reduced astaxanthin content of 30-d-old cyst cells, the astaxanthin production (26.1 ± 1.7 mg/L) improved by 21% compared to the rac-GR24-free control (21.6 ± 1.5 mg/L), owing to improved biomass production. Notably, at the highest dosage of 8 µM rac-GR24, the total fatty acid content of the treated H. pluvialis cells (899.8 pg/cell) was higher than that of the untreated cells (762.5 pg/cell), resulting in a significant increase in the total fatty acid production (361.6 ± 48.0 mg/L; 61% improvement over the control). The ratio of PUFAs, such as linoleic (C18:2) and linolenic (C18:3) acids, among total fatty acids was high (41.5–44.6% w/w) regardless of the rac-GR24 dose.
... In contrast, thin-walled H. pluvialis can use whole cells as a source of astaxanthin, which has potential advantages in maintaining compound stability, easy digestion, enhance bioavailability of carotenoids, and reducing production costs. Astaxanthin is accumulated in H. pluvialis under growthlimiting conditions, such as nutrient deprivation, high light, and/ or high salinity (Lemoine & Schoefs, 2010;Chekanov et al., 2014;Scibilia et al., 2015;Solovchenko, 2015;Oslan et al., 2021). Nitrogen is one of the essential nutrients that affect cell growth and enzymatic activity of H. pluvialis (Zhang et al., 2018). ...
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The dietary supplementation of Haematococcus pluvialis is a natural, safe, and sustainable method for fish pigmentation. However, astaxanthin-rich H. pluvialis cysts have a poor effect in pigmenting salmonid flesh due to their rigid and thick cell wall. H. pluvialis thin-walled motile cells have recently attracted attention due to their potential advantages in maintaining compound stability, easy digestion, enhancing the bioavailability of carotenoids, and reducing production costs. This study aimed to investigate the effect of various nitrogen concentrations and light intensities on astaxanthin production in motile cells. We first investigated the effect of four different concentrations of nitrogen on astaxanthin accumulation in motile cells. According to the results, the motile cells had the highest astaxanthin concentration and content under the 0 N condition. Then, we compared the differences in astaxanthin production in motile cells under three different light intensities under 0 N conditions. The results showed that after four days of treatment, the protoplasts of the motile cells in the medium light (ML) group and the high light (HL) group had distinct granularity. The cell mortality rate in the HL group reached more than 15%, which was significantly higher than that in the low light (LL) and ML groups, indicating that high light intensity was not suitable for inducing motile cells to accumulate astaxanthin. There were no significant differences between the LL and ML groups in astaxanthin content, motile cells percentage, and cell mortality rate. Considering these indicators, we recommended inducing motile cells to produce astaxanthin under low light conditions because it is more economical in terms of electricity consumption. This study added to the knowledge that nitrogen and light affects the accumulation of astaxanthin in H. pluvialis motile cells. The results would help determine the optimal nitrogen and light conditions in astaxanthin production from motile cells.
... However, a special case has to be considered in H. pluvialis. As these algae are able to produce a large amount of astaxanthin under stress conditions, they also turn into red cells (haematocysts) from green (or vegetative) cells with concomitant remodeling of several metabolic reactions, photosynthetic activity and photoprotective capability (e.g., [25][26][27][28][29]). Red cells exhibited enhanced cyclic electron flow [27,28]; in agreement with this observation, we found that red cells exhibit a large wave phenomenon as compared to green cells when treated with HA and microaerobic condition. ...
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... While, the use of high light intensities requires high amount of energy, nutrients restriction is more convenient in terms of cost and implementation for industrial scale production of astaxanthin. Nitrogen starvation is believed to induce astaxanthin production effectively [178][179][180][181]. A recent study showed 25% increase in astaxanthin using a sequential stress strategy involved extended nitrogen starvation followed by moderate light intensity exposure at the late palmella stage [177]. ...
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The global market demand for natural astaxanthin is rapidly increasing owing to its safety, the potential health benefits, and the diverse applications in food and pharmaceutical industries. The major native producers of natural astaxanthin on industrial scale are the alga Haematococcus pluvialis and the yeast Xanthopyllomyces dendrorhous. However, the natural production via these native producers is facing challenges of limited yield and high cost of cultivation and extraction. Alternatively, astaxanthin production via metabolically engineered non-native microbial cell factories such as Escherichia coli, Saccharomyces cerevisiae and Yarrowia lipolytica is another promising strategy to overcome these limitations. In this review we summarize the recent scientific and biotechnological progresses on astaxanthin biosynthetic pathways, transcriptional regulations, the interrelation with lipid metabolism, engineering strategies as well as fermentation process control in major native and non-native astaxanthin producers. These progresses illuminate the prospects of producing astaxanthin by microbial cell factories on industrial scale.
... Such remodeling has been described before [2,3] and is characterized by a dismantling of chloroplasts, accumulation of lipid bodies and appearance of osmiophilic astaxanthin granules which at the later stages fuse with lipid bodies forming 'astaxanthin oil droplets' [2]. However, while the biochemistry of astaxanthin synthesis has been characterized [5], compartmentation of its stages between cell organelles is far from understood. We observed that in many cases the prevailing location of osmiophilic bodies was in single-membrane organelles which resembled autolysosomes; smaller osmiophilic granules were often located in autophagosomes surrounded by double membrane. ...
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The microalga Haematococcus lacustris (formerly H. pluvialis) is able to accumulate high amounts of the carotenoid astaxanthin in the course of adaptation to stresses like salinity. Technologies aimed at production of natural astaxanthin for commercial purposes often involve salinity stress; however, after a switch to stressful conditions, H. lacustris experiences massive cell death which negatively influences astaxanthin yield. This study addressed the possibility to improve cell survival in H. lacustris subjected to salinity via manipulation of the levels of autophagy using AZD8055, a known inhibitor of TOR kinase previously shown to accelerate autophagy in several microalgae. Addition of NaCl in concentrations of 0.2% or 0.8% to the growth medium induced formation of autophagosomes in H. lacustris, while simultaneous addition of AZD8055 up to a final concentration of 0.2 µM further stimulated this process. AZD8055 significantly improved the yield of H. lacustris cells after 5 days of exposure to 0.2% NaCl. Strikingly, this occurred by acceleration of cell growth, and not by acceleration of aplanospore formation. The level of astaxanthin synthesis was not affected by AZD8055. However, cytological data suggested a role of autophagosomes, lysosomes and Golgi cisternae in cell remodeling during high salt stress.
... For a given microorganism, however, the form and composition of astaxanthin are relatively stable. While astaxanthin in the heterobasidiomycetous yeast Xanthophyllomyces dendrorhous (formerly named Phaffia rhodozyma) is in a free form with the (3R,3 ′ R)-isomer [25], the majority of astaxanthin in microalgae is in fatty acid esterified forms [26,27]. It has been reported that astaxanthin esters have a higher thermal stability and higher bioavailability than free-form astaxanthin [28]. ...
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The filamentous microalga Oedocladium carolinianum that was isolated from soil was found to be capable of producing large amounts of astaxanthin under stress culture conditions. When the culture conditions were optimized, the maximum specific growth rate of 0.37 d⁻¹ and the maximum biomass concentration of 10.12 g L⁻¹ were obtained after 18 days of cultivation in 1-liter glass columns (inner diameter: 5 cm) under laboratory conditions. When subjected to culture conditions of nitrogen starvation and 2 g L⁻¹ NaCl-induced salinity stress, however, the cells produced up to 3.91% w/w astaxanthin and a high astaxanthin productivity of 24.2 mg L⁻¹ d⁻¹ was obtained. Analysis by HPLC-MS revealed that the majority of astaxanthin was in fatty acid esterified forms with a typical molecular ratio of free, monoester and diester astaxanthin of 1:18:81. The biosynthesis of astaxanthin coincided with that of fatty acids, and the total fatty acid content reached 40% w/w or more. The technical feasibility of mass culture of O. carolinianum was tested in a 7000-L inclined thin-layer photobioreactor and a 10,000-L tubular photobioreactor in a greenhouse. This demonstrated that O. carolinianum grew rapidly in a nitrogen replete BG-11 culture medium and the maximum biomass concentrations obtained in the inclined thin-layer photobioreactor and tubular photobioreactor were 3.74 g L⁻¹ and 3.07 g L⁻¹ respectively, resulting in maximum biomass productivities of 276 mg L⁻¹ d⁻¹ and 198 mg L⁻¹ d⁻¹, respectively. Although small populations of a few zooplankton species occurred in the two types of photobioreactors, none grazed on O. carolinianum and they grazed on invading unicellular microalgae instead. It was therefore concluded that O. carolinianum is a promising microalga for sustainable co-production of astaxanthin and fatty acids.
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Oxidative stress has been implicated in pathophysiology of aging and age-associated disease. Antioxidative medicine has become a practice for prevention of atherosclerosis. However, limited success in preventing cardiovascular disease (CVD) in individuals with atherosclerosis using general antioxidants has prompted us to develop a novel antioxidative strategy to prevent atherosclerosis. Reducing visceral adipose tissue by calorie restriction (CR) and regular endurance exercise represents a causative therapy for ameliorating oxidative stress. Some of the recently emerging drugs used for the treatment of CVD may be assigned as site-specific antioxidants. CR and exercise mimetic agents are the choice for individuals who are difficult to continue CR and exercise. Better understanding of molecular and cellular biology of redox signaling will pave the way for more effective antioxidative medicine for prevention of CVD and prolongation of healthy life span.
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Microalgae are considered a promising source for various high-value products, including carotenoids and omega-3 and omega-6 polyunsaturated fatty acids (PUFAs). Excluding production by heterotrophic fermentation, only two microalgal high-value products are successfully marketed at a relevant scale: β-carotene from Dunaliella salina, and astaxanthin from Haematococcus pluvialis. In addition, Chlorella and Spirulina biomass are marketed in large volumes as nutraceuticals, and phycocyanin extracted from cyanobacteria has gained major market share recently. Additional algal strains of industrial potential have been described for the production of high-value products, such as carotenoids and PUFAs, or for biofuels production, and novel promising strains continue to be reported. However, phototrophic production of algal products is considered 2–5 times more expensive than competing pathways for both high-value products and bulk biomass. Recent—and often still unpublished—advances have been made in deciphering the genomes and transcriptomes of multiple high-value algal species and their metabolic pathways toward carotenoid,lipid, and PUFA biosynthesis have been resolved. Together with recent progress in microalgae transformation and genetic engineering, it is now possible to increase production efficiencies for high-value products, bulk biomass, and biofuels in microalgae by metabolic engineering. Furthermore, encouraging progress has been achieved in expressing high-value proteins in several microalgae species. This review describes major, recent advances in the understanding and engineering of microalgal metabolic pathways towards developing competitive production pathways. Such technologies, supported by adequate biorefinery technologies and highly sustainable cultivation options, can significantly contribute to enabling sustainable production of high-value biobased chemicals, while also offering opportunities for increasing sustainable food and fuel supplies. Microalgae thus offer the unique opportunity to shift significant agricultural production volumes into unproductive land using non-potable water while reducing global resource depletion and pollution from unsustainable farming and fishing practices.
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Morphometric and physiologic-biochemical characteristics as well as the productivity of the green terrestrial microalga Bracteacoccus minor were investigated in a two-stage batch culture. The specific adaptive responses, which the microalga developed under the experimentally induced secondary carotenogenesis were: high cellular resistance to sodium acetate, the accumulation of a multicomponent mixture of secondary ketocarotenoids with the dominance of astaxanthin diesters (37–42% of the total carotenoids) and a large lipid content in algal biomass (53–63% of dry matter).
Chapter
Research into non-photochemical quenching of chlorophyll fluorescence (NPQ), as an indicator of the thermal dissipation of excess excitation energy, has involved a number of different of approaches, drawing technology and intellect from a wide range of disciplines, from physics and chemistry through to ecology and agronomy. The timing of these approaches owed much to developments and advances occurring in these disparate areas of science, but also depended on the gradual evolution of our thinking about what NPQ was, what function it reflects, how its measurement could be exploited and ultimately what its molecular mechanism could be. The foundations of our current knowledge were laid 40 years ago, following intensive investigation of the bioenergetics of the thylakoid membrane, and the seminal papers on which this was based are described here. As our understanding of the biochemistry and biophysics of the thylakoid membrane increased, new ideas about NPQ emerged. The light-harvesting complexes of photosystem II and the xanthophyll cycle carotenoids bound to them have been the central features of NPQ research during the last 20 years. Outstanding advances in molecular genetics, in structural biology, in time-resolved spectroscopy and in the extraction and purification of membrane proteins all had major influence. The development of two main NPQ theories was the hallmark of this period. First, the idea that the process underlying NPQ was intrinsic to the main LHCII trimers, that these complexes can switch between unquenched to quenched states, that the equilibrium between these states is dependent on protonation and de-epoxidation of the xanthophyll cycle carotenoids and that the process is ultimately governed by the dynamic macrostructure of the grana membranes. Second, the notion of zeaxanthin as the specific obligatory quencher, bound to internal sites in the monomeric minor complexes (the gateways between the “bulk” LHCII antenna and the PS II cores), the activation of which is triggered by protonation of the PsbS protein. The emergence of these ideas is described, critical experiments highlighted and prospects for future progress assessed. The article concludes with a note on the ecological and agricultural implications of NPQ.
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Mass culture of Haematococcus pluvialis is commercially available and astaxanthin derived from H. pluvialis is used as a nutraceutical for human health and as a coloring agent for aquaculture. However, the Haematococcus industry has achieved only moderate success due to low astaxanthin productivity and the high production cost associated with the current mass culture systems and processes. This chapter provides the current understanding of biology of H. pluvialis from molecular, cellular, and physiological aspects with an emphasis on the pathway for and physiological role of astaxanthin synthesis in response to photooxidative stress. Several major biological and environmental factors that affect growth and astaxanthin production are identified, of which fungal contamination is the most detrimental factor that is largely responsible for low astaxanthin production and frequent culture crashes. Future expansion of the Haematococcus industry will depend on significant improvement in our knowledge about the biology of Haematococcus and predator–prey interaction, and transformation of the knowledge into a next-generation mass culture system and process including an advanced Haematococcus crop protection program.
Article
The unicellular green alga Haematococcus pluvialis Flotow is known for its massive accumulation of ketocarotenoids under various stress conditions. Therefore, this microalga is one of the favored organisms for biotechnological production of these antioxidative compounds. Astaxanthin makes up the main part of the secondary carotenoids and is accumulated mostly in an esterified form in extraplastidic lipid vesicles. We have studied phytoene desaturase, an early enzyme of the carotenoid biosynthetic pathway. The increase in the phytoene desaturase protein levels that occurs following induction is accompanied by a corresponding increase of its mRNA during the accumulation period, indicating that phytoene desaturase is regulated at the mRNA level. We also investigated the localization of the enzyme by western-blot analysis of cell fractions and by immunogold labeling of ultrathin sections for electron microscopy. In spite of the fact that secondary carotenoids accumulate outside the chloroplast, no extra pathway specific for secondary carotenoid biosynthesis in H. pluvialis was found, at least at this early stage in the biosynthesis. A transport process of carotenoids from the site of biosynthesis (chloroplast) to the site of accumulation (cytoplasmatic located lipid vesicles) is implicated.