Cymbopogon Species; Ethnopharmacology, Phytochemistry and the Pharmacological Importance

Abstract

Cymbopogon genus is a member of the family of Gramineae which are herbs known worldwide for their high essential oil content. They are widely distributed across all continents where they are used for various purposes. The commercial and medicinal uses of the various species of Cymbopogon are well documented. Ethnopharmacology evidence shows that they possess a wide array of properties that justifies their use for pest control, in cosmetics and as anti-inflammation agents. These plants may also hold promise as potent anti-tumor and chemopreventive drugs. The chemo-types from this genus have been used as biomarkers for their identification and classification. Pharmacological applications of Cymbopogon citratus are well exploited, though studies show that other species may also useful pharmaceutically. Hence this literature review intends to discuss these species and explore their potential economic importance.

Full text

Molecules 2015, 20, 7438-7453; doi:10.3390/molecules20057438
molecules
ISSN 1420-3049
www.mdpi.com/journal/molecules
Review
Cymbopogon Species; Ethnopharmacology, Phytochemistry and
the Pharmacological Importance
Opeyemi Avoseh 1, Opeoluwa Oyedeji 1,*, Pamela Rungqu 1, Benedicta Nkeh-Chungag 2 and
Adebola Oyedeji 3
1 Chemistry Department, University of Fort Hare, 5700 Alice, South Africa;
E-Mails: seavoseh@gmail.com (O.A.); 200800815@ufh.ac.za (P.R.)
2 Department of Zoology, Walter Sisulu University, 5099 Mthatha, South Africa;
E-Mail: bnkehchungag@wsu.ac.za
3 Department of Chemistry, Walter Sisulu University, 5099 Mthatha, South Africa;
E-Mail: aoyedeji@wsu.ac.za
* Author to whom correspondence should be addressed; E-Mail: ooyedeji@ufh.ac.za;
Tel.: +27-764260280.
Academic Editor: Luca Forti
Received: 25 January 2015 / Accepted: 25 March 2015 / Published: 23 April 2015
Abstract: Cymbopogon genus is a member of the family of Gramineae which are herbs
known worldwide for their high essential oil content. They are widely distributed across all
continents where they are used for various purposes. The commercial and medicinal uses of
the various species of Cymbopogon are well documented. Ethnopharmacology evidence
shows that they possess a wide array of properties that justifies their use for pest control, in
cosmetics and as anti-inflammation agents. These plants may also hold promise as potent
anti-tumor and chemopreventive drugs. The chemo-types from this genus have been used as
biomarkers for their identification and classification. Pharmacological applications of
Cymbopogon citratus are well exploited, though studies show that other species may also
useful pharmaceutically. Hence this literature review intends to discuss these species and
explore their potential economic importance.
Keywords: Cymbopogon; ethnopharmacology; secondary metabolites; terpenes; chemo-types
OPEN ACCESS

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1. Introduction
The presence of secondary metabolites in plants is characterized by their ability to provide defenses
against biotic and abiotic stress [1]. The mechanism of defense varies from plant to plant, their
environmental conditions and climatic variations. However, the presence of these metabolites in plant
are usually in minimum amounts though several molecular techniques are available to either increase or
decrease the quantity of a particular metabolite by blocking competitive pathways and enriching
metabolites of choice [2]. Terpenes, alkaloids (N-containing compounds) and phenolics constitute the
largest groups of secondary metabolites. The shikimic acid pathway is the basis of the biosynthesis of
phenolics while the terpenes which are comprised of isoprene units arise from the mevalonate pathway [3].
Aspirin (1) from white willow, quinine (2) from the cinchona plant and artemisinin (3) from Artemisia
annum are all plant secondary metabolites. The biological application of these metabolites as therapeutic
agents for a broad spectrum of ailments and the microbial infections has been salutary in human history.
The genus Cymbopogon is widely distributed in the tropical and subtropical regions of Africa, Asia
and America. Comprised of 144 species, this genus is famous for its high content of essential oils which
have been used for cosmetics, pharmaceuticals, and perfumery applications [4]. Two main species,
C. flexuosus and C. citratus (lemongrass) are commercially cultivated in the Democratic Republic of
Congo (DRC), Madagascar, and the Comoros Island. However, the leading exporter of these plants is
Guatemala, trading about 250,000 kg per year and while the USSR sells about 70,000 kg per year [5].
The commercial value of some Cymbopogon species is further enhanced by their ability to grow in
moderate and extremely harsh climatic conditions [6]. In environments where they are not used for
cosmetics, drug or perfumery, such as in the Eastern Cape Province of South Africa, these plants have
found a good application as roof thatches and grass brooms [7].
2. Ethnopharmacology of Cymbopogon Species
Traditional applications of Cymbopogon genus in different countries shows high applicability as a
common tea, medicinal supplement, insect repellant, insecticide, in flu control, and as
anti-inflammatory and analgesic. Table 1 shows the common names of some species, their relevance and
how they are applied. C. citratus is ranked as one of the most widely distributed of the genus which is
used in every part of the world. Its applications in Nigeria include cures for upset stomach, malaria
therapy, insect repellent and as an antioxidant (tea) [8]. C. citratus and C. flexuosus are the prevailing
species in Eastern and Western India and have been used locally in cosmetics, insecticides, and for the
treatment of digestive disorders and fevers [9,10].

Molecules 2015, 20 7440
Table 1. Several Cymbopogon species, common name, regions, plant part used and the uses.
Species Region Common Name Parts Medicinal Uses References
C. nardus (L.) Rendle India Citronella oil Leaves Insect repellent and as perfumes [11]
C. parkeri Stapf Pakistan Lemon grass Aerial Antiseptic and stomachic treatment [12]
C. excavatus Hoscht South Africa Bread-leavened
Turpentine grass Sheaths Used as insecticides [13]
C. olivieri (Boss) Pakistan Pputar Aerial Pyretic, vomit, diuretic, rheumatism, and as anti-malaria condiment. [14,15]
C. validus (Stapf) Eastern and
Southern Africa African bluegrass Essential oils skin toner, anti-ageing in men, fumigant and for rodent control [16]
C. winterianus (Jowitt) Brazil Java grass Fleshy leaves Treatment of epilepsy and anxiety [17]
C. marginatus (Steud.) South Africa Lemon-Scented grass Root They are used as moth repellent [18]
C. citratus Stapf
India Lemon grass Aerial Fever, digestive disorders [9]
Nigeria Lemon grass Leaves Diabetes, inflammation and nerve disorders [8]
Argentina Limonaria Leaves Against cold and flu, and digestive complaints, stomach
upsets and as decoction with other plants for malaria
[19]
Cuba Cana Santa Leaves [20]
Costa Rica Grass tea Leaves To relieve cough, carminative, expectorant and depurative [21]
Colombia Limonaria Rhizome It is chewed and used as toothbrush and for pest control. [22,23]
Brazil Capimsanto Leaves Anxiolytic and anti-hypertensive [24]
Trinidad &
Tobago “fever grass” Grass and rhizomes The teas from it are used to treat cold, flu, fever and diabetes [24]
C. giganteus
(Hochst.) Chiov. Cameroon Tsauri grass
decoctions of leaves
and flowers Cough and arterial hypertension [25]
C. ambiguous
(Hack.) A. Camus. Australia Native Lemon Grass Leaves and stems Headache remedy, chest infections, muscle cramp and Scabies [26,27]
C. procerus
(R.Br.) Domin Australia Scent grass Leaves and stems Leaves and stem are pounded and used as medicinal body wash;
used for headache [28]
C. flexuosus
(Nees ex Steud.) Wats. India Lemon grass Leaves Cosmetics, antiseptic and for treatment of fever [10]

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Table 1. Cont.
Species Region Common Name Parts Medicinal Uses References
C. pendulus
(Nees ex Steud.) Wats. India Jammu Lemongrass Leaves Antiseptic and for perfumery [29]
C. scheonanthus
(L.) Spreng Saudi Arabia Ethkher Leaves Antidiarrheal, to treat fever, treatment of jaundice and tonic [30]
C. obtectus (S.T. Blake) Central Australia Silky-heads Mixture Cold and flu, headaches, fever and sore throat [27]
C. proximus (Stapf.) Egypt Halfabar Leaves Expulsion of renal and ureteric calculi [31]
Cymbopogon refractus
(R.Brown) A. Camus. Australia Barbed wire grass Leaves Feed for animals [32]
C. densiflorus
(Steud.) Stapf Congo Lemongrass Leaves and rhizome
Employed against asthma, epilepsy, abdominal cramps and
pains and also for interpreting dreams by witch doctors. [33,34]
C. jwarancusa
(Jones) Schult. Egypt Thé Limon The whole plant Condiment and for medicinal purpose [35]

Molecules 2015, 20 7442
In the Middle East, C. olivierri and C. parkeri are more predominant, and they are used as antiseptics,
anti-malarial condiments, diuretics and also to cure rheumatism [12,14,15]. The high amounts of volatile
compounds from these species are responsible for their diverse uses.
Figure 1. Flavonoids and triterpenoids from Cymbopogon species.
OH
O
O
O
N
OH N
OO
O
H
O
OO
H
O
OH
OH
OOH
O
OH
OH
OH
OH
OCH3
OH
OCH3
O
OOH
OH
OH
OH
O
OOH
OH O
OH
OH
OOH
O
O
OH
OH
OH OH
O
O
OH
OOH
O
O
OH
OH
OH OH
O
OH
OH
OOH
OH
O
OH
OH
OH O
O
OH
OH
OH
O
OH
OH
OH
OOH
OH O
O
OH
OH
OH
OH
O
O
OH
OH
OH
H3CO
H3CO
OCH3OH
OH
CO2H
OH
OH
OHO
O
OH
OH
OH
OH
OH
O
OH
OH OH
OCH3
OH
OCH3
OCH3
OCH3
OCH3
OCH3
OCH3
OCH3
O
O
OH
OH
H
H
H
O
H
H
H
OH
O
O
O
OH OH
H
H
OH
OCH3
OCH3
H3CO
H
OH
O
1
2
34
5
6789
10 11 12 13
14
15 16
17 18
19
20 21 22
23
24
25 26 27 28 29
30
31
32 33
34 35

Molecules 2015, 20 7443
3. Phytochemistry
The enormous information gathered from the ethno-pharmacological applications of Cymbopogons
begged the investigation of its chemical constituents. These studies have led to the isolation of alkaloids,
volatile and non-volatile terpenoids, flavonoids, carotenoids and tannins from every part of these plants.
Figure 1 displays some of the compounds isolated from Cymbopogon species.
3.1. Alkaloids
The rhizome of C. citratus from Nigeria was reported to contain about 0.52% alkaloids from 300 g
plant material [36].
3.2. Flavonoids
This class of compounds has potent antioxidant properties. Some of the flavonoids isolated from
Cymbopogon species are presented in Figure 1. Isoorientin (4) and tricin (5) were isolated from the
dichloromethane extract of C. parkeri [37], evaluation of these two compounds revealed their muscle
relaxation activity [38]. Isolation of luteolin (6), luteolin 7-O-glucoside (cynaroside) (7), isoscoparin (8)
and 2''-O-rhamnosyl isoorientin (9) from the leaves and rhizomes of C. citratus has been reported. Other
flavonoid compounds isolated from the aerial parts of C. citratus are quercetin (10), kaempferol (11) and
apigenin (12) [39], isolated elimicin (13), catechol (14), chlorogenic acid (15), caffeic acid (16) and
hydroquinone (17) from the aerial parts of the same species. Isolation of 4-phenylpropanoids from
Australian species of C. ambiguus has been reported. These compounds are eugenol
(4-allyl-2-methoxyphenol) (18); elemicin (5-allyl-1,2,3-trimethoxybenzene) (19); eugenol methylether
(4-allyl-1,2-dimethoxybenzene) (20) and trans-iso-elemicin (1,2,3-trimethoxy-5-(1-propenyl) benzene)
(21) and all these isolates exhibited good inhibition activity against ADP-induced human platelet
serotonin release which is associated with headaches [26].
3.3. Cymbopogon Terpenoids
3.3.1. Non-Volatile Terpenoids
Plants in the Cymbopogon genus contain large amounts of volatile terpenoids though a few species
from this genus are reported to contain non-volatile terpenoids as well. Bottini et al. [40] isolated a novel
bis-monoterpenoid named cymbodiacetal (22) from C. martinii. The triterpenoids cymbopogone (23)
and cymbopogonol (24) (Figure 1) were also reported from the leaves of C. citratus [41].
3.3.2. Volatile Terpenoids of Cymbopogon Species
Different chemotypes of Cymbopogon species contain varying major compounds such as citral,
geraniol, citronellol, piperitone and elemin (Table 2). In the literature, the majority of the C. citratus
analysed showed a remarkably high percentage of neral (25) and geranial (26). Analysis of C. citratus
species from Brazil [42], India [43], West and Eastern Africa [43–49] and Asia [50] showed the high
value of neral and geranial chemotypes. A special distinguishing feature between C. citratus of African
origin is the high amount of myrcene observed in them [44–49]. High occurance of piperitone (27)

Molecules 2015, 20 7444
characterizes the oils of C. parkeri and C. olivieri from Iran. Jiroveltz et al. [25] reported a significant
presence of cis-p-mentha-1(7),8-dien-2-ol (28) and its isomer trans-p-mentha-1(7),8-dien-2-ol (29) in the
oils of C. giganteus from Cameroon [25]. Predominant components observed in other Cymbopogon
species essential oils from around the world include δ-2-carene (30) in C. proximus from Cameroon [51],
linalool (31) from Malaysia’s C. nardus [52], limonene (32) in C. schoenanthus (Tunisia) and
C. giganteus (Burkina Faso) [46] and elemicin (33) from the oils of C. pendulus from India [53].
Observation of the oil of C. winterianus from different parts of Brazil showed two major chemotypes
based on the amount of geraniol (34) and citronellal (35) [17,54–56].
Table 2. Major components observed in some Cymbopogon species.
Compound Species Country/Region Major % References
cis-p-mentha-1(7),8-dien-2-ol (C10H16O) C. giganteus(F)
Cameroon 22.8 [25]
Burkina Faso 12.0 [46]
Madagascar 19.0 [57]
trans-p-mentha-1(7),8-dien-2-ol
C. giganteus Cameroon 26.5 [25]
C. giganteus Burkina Faso 14.2 [46]
C. densiflorus Zambia 11.1 [57]
C. giganteus Madagascar 22.4 [56]
Limonene (C10H16)
C. giganteus Cameroon 7.4 [25]
C.giganteus Burkina Faso 42.0 [46]
C. proximus Burkina Faso 3.9 [51]
C. schoenanthus Tunisia 24.2 [58]
Elemicin (C12H16O3) C. pendulus India 53.7 [53]
α-Pinene (C10H16) C. pendulus India 6.1 [53]
Camphene (C10H16) C. pendulus India 9.1 [53]
C.winterianus India 8.0 [59]
Geranial (C10H16O)
C. flexuosus India (Kumauon region) 33.1 [60]
India (Bilhar) 42.4 [43]
C. citratus
Burkina Faso 48.1 [46]
Brazil 50.0 [42]
Egypt 40.72 [61]
Zambia 39.0 [47]
Kenya 39.53 [57]
Benin republic 27.04 [62]
Nigeria 33.7 [44]
Angola 40.55 [63]
Congo Brazaville 48.88 [45]
Ivory Coast 34.0 [45]
Mali 45.3 [45]
Iran 39.16 [50]
C. winterianus S.E. Brazil 8.05 [55]

Molecules 2015, 20 7445
Table 2. Cont.
Compound Species Country/Region Major % References
Neral (C10H16O)
C. flexuosus
India 30.0 [60]
Burkina Faso 34.6 [46]
India (Bilhar) 29.8 [43]
Brazil (North) 30.1 [42]
Egypt 34.98 [61]
Zambia 29.4 [47]
Kenya 33.31 [48]
C. giganteus Benin republic 19.93 [62]
Nigeria 26.5 [44]
C. citratus
Angola 28.26 [63]
Malaysia 50.81 [64]
Congo Brazzaville 36.24 [49]
Brazil 4.53 [17]
Ivory Coast 32.5 [45]
Mali 26.3 [45]
Iran 30.95 [50]
Geranyl acetate (C12H20O2) C. flexuosus India 12.0 [60]
Linalool (C10H18O)
C. flexuosus India 2.6 [60]
C.winterianus India 1.5 [59]
C. martini India 2.0 [65]
C. nardus Malaysia 11.0 [52]
Geraniol (C10H18O)
C. winterianus India 23.9 [59]
C. martinii India 84.16 [65]
C. winterianus Brazil 32.82 [17]
Brazil (para state) 16.2 [54]
C. winterianus S.E Brazil 40.06 [55]
Citronellal (C10H18O)
C.winterianus India 32.7 [59]
C. nardus Malaysia 29.6 [52]
C. winterianus Brazil 36.19 [17]
C. winterianus Brazil (para state) 26.5 [54]
C. winterianus S.E. Brazil 27.44 [55]
Citronellol (C10H20O)
C. winterianus India 15.9 [59]
C. winterianus Brazil 11.34 [17]
C. winterianus Brazil (Para state) 7.3 [54]
C. winterianus S.E. Brazil 10.45 [55]
Myrcene (C10H16)
C. citratus
C. citratus
C. citratus
Burkina Faso 11.0 [46]
Egypt 15.69 [61]
Zambia 18.0 [47]
Benin republic 27.83 [62]
Nigeria 25.3 [44]
Angola 10.57 [63]
Ivory Coast 18.1 [45]
Mali 9.1 [45]

Molecules 2015, 20 7446
Table 2. Cont.
Compound Species Country/Region Major % References
Selina-6-en-4-ol (C15H26O) C. citratus Brazil 27.8 [42]
α-Cadinol (C15H26O) C. citratus Brazil 8.2 [42]
Piperitone (C10H16O)
C. olivieri Iran 72.8 [14]
C. parkeri Iran 80.8 [12]
C. proximus Burkina Faso 59.1 [51]
4-Carene (C10H16) C. olivieri Iran 11.8 [12]
Germacrene-D (C15H24) C. parkeri Iran 5.1 [11]
δ-2-Carene (C10H16) C. proximus Burkina Faso 22.3 [51]
β-Phellandrene (C10H16) C. schoenanthus Tunisia 13.4 [58]
3.4. Tannins
A literature search on the phytochemical screening of C. citratus also reveals the presence of tannins,
however, very little effort has been made in the isolation of these compounds despite the appreciable
amounts reported through quantitative phytochemical tests. Figueirinha et al. fractionated extracts of the
species collected from Portugal and reported about 10 mg dry weight of hydrolysable tannins
(prothocyanidins) [66] while C. citratus from Nigeria showed about 0.6% of tannins [36]. C. citratus is
the single species of Cymbopogon which is most exploited for its tannin content.
4. Pharmacology
Several bioassays have confirmed the potency of Cymbopogon species for their several uses
(Table 3). C. citratus was found to have chemoprotective activity by preventing of diethylnitrosamine
(DEN)-initiated hepatocellular lesions in rats [67]. In South Africa, extract from C. citratus was applied for
treatment of oral thrush in patients who tested positive to HIV/AIDS and proved effective [68].
Insecticidal activity is one of the biological effects of most plant of the Cymbopogon genus; it is either
applied as pest control for stored crops or as mosquito repellent/ insecticide. The essential oils of
C. martinii have been studied and found to display high anthelmintic activity against Caenorhabditis
elegans at ED50 value of 125.4 µg/mL, C schoenanthus, C. giganteus and C. citratus essential oils
from Benin Republic in West Africa all displayed about 100% mortality rate against adult Anopheles
gambiae [69]. The essential oil from C. winterianus caused a dose dependent mortality of Culex
quinquefasciatus with LC50 of 0.9% [70].
The anticancer properties of Cymbopogon species have also been studied. The essential oils of
C. flexuosus was effective in inhibiting the growth and killing of Ehrlich and Sarcoma-180 tumors cells.
In this study, it was discovered that at a dose of 200 mg/kg, Ehrlich solid tumor inhibition was about
57.83% compared to the 45.23% inhibition observed with 5-fluorouracil (22 mg/kg) [71]. Inhibition of
early phase of hepatocarcinogenesis was also observed in C. citratus [67]. Positive results in several other
bioassays such as antiprotozoal, anti-inflammatory, antimicrobial, anti-bacterial, anti-diabetic,
anticholinesterase, molluscidal, antifungal and larvicidal activity are also prominent with Cymbopogon
species as outlined in Table 3.

Molecules 2015, 20 7447
Table 3. Pharmacological evidence of some Cymbopogon species.
Cymbopogon Species Pharmacology Activity References
C. citratus
Cytotoxicity Shows high toxicity against Chinese Hamster Ovary (CHO) cells (IC50 = 10.63 μg/mL) and moderately
toxic against human fibroblast cell line 138 (W138) cells (IC50 = 39.77 μg/mL). [72]
Insecticidal LC50 of 48.6 μL/L against housefly larvae [43]
Neurobehavioral effects Ability to be active as sedative, anxiolytic and anticonvulsant agent [73]
Antitrypanosomal Modest activity against Trypanasoma brucei IC50 = 1.837 ± 0.13 μg/mL [72]
Anti-diabetic Shows activity against poloxamer-407 induced type 2 diabetic (T2D) in Wistar rats [43]
HIV/AIDS As a highly effective control for oral thrush in HIV/AIDS victims in South Africa [68]
Larvicidal activity It shows high inhibition and mortality rate against larva of A. aegypti [74]
Chemopreventive activity Inhibits the early phase of hepatocarcinogenesis in rats [67]
Anti-inflammations Hexane extract inhibited iNOS (inducible nitric oxide synthase)expression, NO (nitric oxide)
production and various LPS (lipopolysaccharide)-induced pathways [75]
C. schoenanthus
Antioxidant(DPPH) 36%–73.8% activity per 2 μL of oil [58]
Acetylcholinesterase inhibitory IC50 = 0.26 ± 0.03 mg mL−1 [58]
Insecticidal activity 2.7 μL/L obtained for LC50 against Callosobruchus maculatus [76]
C. winterianus
Moluscidal LC90 = 97.0mg/L and LC50 = 54.0 mg/L [54]
Larvicidal LC
50 = 181.0mg/L [54]
Anti-fungal Inhibited the growth of 15 strains of Candida albicans at concentrations of 625 μg/mL and 1250 μg/mL [77]
C. giganteus
Antimicrobial High activity against gram +ve and gram −ve bacteria [25]
Cytotoxicity Low cytotoxicity against CHO cells and the human non cancer fibroblast cell line (W138) [72]
Anti-trypanosomal IC50 = 0.25 ± 0.11 μg/mL against Trypanasoma brucei [72]
Antiplasmodial High activity with an IC50 ≤ 20 μg/mL [72]
C. pendulus Antifungal Strong activity against Microsporum audouinii, Trichophyton rubrum and Epidermophyton floccosum at
100% for all the species [78]
C. flexuosus Chemopreventive Potent in vivo activity against Ehrlich and Sarcoma-180 tumors. [71]
C. densiflorus Stapf Antibacterial Gram-negative bacteria. MICs were found to be between 250 and 500 ppm for the
Gram-positive and between 500 and 1000 ppm for the Gram-negative bacteria [79]
C. ambiguus Inflammatory Inhibition of ADP-induced human platelet serotonin release in the cell. [26]
C. nardus Antibacterial MIC values ranged from 0.244 µg/mL to 0.977 µg/mL when tested against the bacterial isolates [52]
C. nervatus Molluscidal activity It inhibits Biomphalaria pfeifferi at LD50 of 213.099 ppm dose dependent [80]
C. olivieri Antimicrobial activity Exhibited excellent antimicrobial activity against gram ±ve organisms [14]

Molecules 2015, 20 7448
5. Conclusions
Cymbopogon species have been used as traditional medicine in many countries. Of all the species
reviewed, C. citratus and C. flexuosus are the most widely used in traditional and in conventional
medicine due to the pharmacological potential of their phytochemicals. The majority of these species
contain a voluminous amount of essential oils which have shown several biological activities such as
insecticidal, anti-protozoan, anticancer, anti-HIV, anti-inflammatory and anti-diabetes effects.
Acknowledgments
The authors are grateful to Govan Mbeki Research office, UFH, Directorates of Research and
Development, WSU and NRF for financial support.
Author Contributions
Opeyemi Avoseh carry out the literature survey and wrote part of first draft of the manuscript. Pamela
Rungqu investigated the essential oil composition of Cymbopogon species found in the Eastern Cape
and wrote part of the first draft of the manuscript. Opeoluwa Oyedeji, Benedicta Nkeh-Chungag and
Adebola Oyedeji are supervisors to the above authors on the chemistry and inflammatory studies of the
essential oils. They also contributed editorial to the writing and editing of the final manuscript
Conflicts of Interest
The authors declare no conflict of interest.
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