No full-text available
To read the full-text of this research,
you can request a copy directly from the authors.
Cultural characteristics of Pholiota adiposa according to substrates composition of sawdust medium by bottle cultivation
Abstract
This study was carried out to develop optimum sawdust medium for bottle cultivation of Pholiota adiposa. Five kinds of sawdust media were tested. Period of primordial formation was shorter 1-5 days in T3 and in T4 than in other treatments. Total cultivation period was 3-5 days longer in treatments including oak sawdust than in T5, treatment of poplar+rice bran(8:2). Stipe diameter, stipe length, and number of valid stipe were the biggest in T3. Yield of fruiting body was increased 33% in T3 and 12% in T4, respectively compared to T1 as control. Therefore, T3, treatment of oak sawdust+beet pulf+cottonseed meal(5:3:2), would be appropriate for the commercial production of Pholiota adiposa by bottle cultivation.
i>Pholiota adiposa is an important edible and medicinal mushroom with high nutritional and medicinal effects. The fruiting body of wild fungi collected from Mudanjiang City, Heilongjiang Province, was identified by morphological description and molecular identification, the biological characteristics and domestication of the fungus was determined by single factor and orthogonal tests. The wild strain isolated was determined to be Ph. adiposa based on morphological characteristics, sequence alignment between ITS and nLSU, and phylogenetic relationship analysis. The single factor results revealed that the optimal carbon source, nitrogen source, culture temperature, and pH for the mycelia growth of Ph. adiposa were glucose, yeast paste, 25°C, and pH 6.5-7.0, respectively. Orthogonal test showed that the optimal formula for mycelia culture was fructose 20 g/L, yeast extract 6 g/L, KH<sub>2</sub>PO<sub>4</sub> 1 g/L and MgSO<sub>4</sub> 2.5 g/L. The highest single bag yield of the fruiting body of Ph. adiposa was 24.96 g in the culture medium formula of sawdust (20%), wheat bran (10%), soybean powder (1%), and quicklime powder (1%). The results will provide basic information for the protection, utilization and domestication of the resources of Ph. adiposa.</i
Objective:
Pholiota adiposa is a valuable edible and medicinal fungus. In this research, Ergosta-4, 6, 8(14), 22-tetraen-3-one (ETO) was obtained from Pholiota adiposa which is the first time to study the anti-diabetic and related mechanism.
Methods:
Ergosta-4, 6, 8 (14), 22-tetraen-3-one (ETO) was defined by IR and NMR. Relevant biochemical indicators were detected by ELISA assay, hematoxylin-eosin staining (H & E), fasting glucose levels (FBG), oral glucose tolerance test (OGTT), tissue homogenate biochemical measurements, immunohistochemical staining and western blot.
Results:
In this research, the ETO treatment groups exhibited a significant reduction in fasting blood glucose (FBG) levels, the High dose group (HD) was about 10 mmol/L lower than the diabetic control group (DC), and increase in body weight, the HD group weighed about 5 g more than the DC group on average. Also, the levels of triacylglycerol (TG), total cholesterol (TC), and low-density lipoprotein-cholesterol (LDL-C) were found to be decreased, while the levels of high-density superoxide dismutase (SOD), lipoproteincholesterol (HDL-C), catalase (CAT), and glutathione peroxidase (GSH-Px) were increased in the ETO treatment groups. The pancreatic and liver sections of diabetic control group (DC) exhibited several histopathological changes, but the ETO treatment groups exhibited improvements. ETO treatment led to the significant restoration of islet morphology and function. Moreover, the results of the western blot analysis indicate that ETO could be used for the treatment of diabetes, since it modifies part of the IRS1 / PI3K / AKT signaling pathway.
Development of substrate for increasing yield of Pholiota adiposa, Geumbongee. Research and Development Report of Chungcheongbuk-Do ARES
- Wb Chang
- Bk Kang
- Sk Park
- Ts Kim
Analysis of genetic relationship and physiological activity between Geumbongee and Pholiota spp
- Wb Chang
- Bk Kang
- Sk Park
- Ts Kim
- Js Cha
Recycling post-harvest medium from bottle cultivation for oyster mushroom(Pleurotus ostreatus)
- Jc Cheong
- Cj Lee
- Pg Shin
- Js Suh
Development of storage method and processed foods using Pholiota adiposa
- Ks Kim
- Sj Joo
- Hs Yoon
- Kj Choi
- Sk Park
- Ts Kim
Study of medium material for bottle culture of Pholiota adiposa
- Wb Chang
- Cs Shin
- Sk Park
- Ts Kim
- Js Cha
Cultivation method and characterization of commercial strain 'Jinhwang' in Pholiota adiposa
- Ws Kong
- Yb Yoo
- Cs Jhune
- Ch You
- Yh Cho
- Wb Chang
- Kh Kim
Study on physiolosical characteristics and artificial cultivation of Pholiota adiposa
- Cs Shin
- Wb Chang
- Hk Lee
- Kb Min
- Js Cha
- Sh Yu
Development of substrate for increasing yield of Pholiota adiposa
- W B Chang
- B K Kang
- S K Park
- Kim Ts
Chang WB, Kang BK, Park SK, and Kim TS. 2005a.
Development of substrate for increasing yield of Pholiota
adiposa, Geumbongee. Research and Development Report
of Chungcheongbuk-Do ARES. pp. 449-461.
Analysis of genetic relationship and physiological activity between Geumbongee and Pholiota spp
- W B Chang
- B K Kang
- S K Park
- Kim Ts
- J S Cha
Chang WB, Kang BK, Park SK, Kim TS and Cha JS.
2005b. Analysis of genetic relationship and physiological
activity between Geumbongee and Pholiota spp. Research
and Development Report of Chungcheongbuk-Do ARES.
pp. 440-448.
Study of medium material for bottle culture of Pholiota adiposa
- W B Chang
- C S Shin
- S K Park
- T S Kim
- J S Cha
Chang WB, Shin CS, Park SK, Kim TS, and Cha JS.
2002. Study of medium material for bottle culture of
Pholiota adiposa. Research and Development Report of
Chungcheongbuk-Do ARES. pp. 428-436.
Recycling post-harvest medium from bottle cultivation for oyster mushroom(Pleurotus ostreatus)
- J C Cheong
- C J Lee
- P G Shin
- J S Suh
Cheong JC, Lee CJ, Shin PG, and Suh JS. 2012. Recycling
post-harvest medium from bottle cultivation for oyster
mushroom(Pleurotus ostreatus). J. Mushroom Sci. Prod.
10(4): 167-173.
Development of storage method and processed foods using Pholiota adiposa
- K S Kim
- S J Joo
- H S Yoon
- K J Choi
- S K Park
Kim KS, Joo SJ, Yoon HS, Choi KJ, Park SK, and Kim TS.
2003. Development of storage method and processed
foods using Pholiota adiposa. Research and Development
Report of Chungcheongbuk-Do ARES. pp. 368-384.
Cultivation method and characterization of commercial strain 'Jinhwang' in Pholiota adiposa
- W S Kong
- Y B Yoo
- C S Jhune
- C H You
- Y H Cho
- W B Chang
Kong WS, Yoo YB, Jhune CS, You CH, Cho YH, Chang
WB, and Kim KH. 2006. Cultivation method and
characterization of commercial strain 'Jinhwang' in
Pholiota adiposa. J. Mushroom Sci. Prod. 4(3): 106-110.
National Institute of Horticultural & Herbal Science. 2009.
Interpretation of Korean mushroom varieties. Nature-Human Publishing Co. p. 132.
Study on physiolosical characteristics and artificial cultivation of Pholiota adiposa
- C S Shin
- W B Chang
- H K Lee
- K B Min
- J S Cha
Shin CS, Chang WB, Lee HK, Min KB, Cha JS, and Yu
SH. 2000. Study on physiolosical characteristics and
artificial cultivation of Pholiota adiposa. Research and
Development Report of Chungcheongbuk-Do ARES. pp.
323-333.