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Abstract

TiO 2 showed Human exposure to PAHs is almost inevitable. They are produced during the combustion processes of organic materials during industrial and other human activities, like processing of coal and crude oil, vehicle traffic and cigarette smoking. A number of PAHs like Benzo[alpha]Pyrene are known to cause lung cancer. We investigated, if nanoparticles could somehow provide protection against the BaP induced toxicity. In this study we evaluated the protective potential of UF-TiO 2 against BaP induced toxicity by Wet lab and in silico experiments. BaP enters in the cell via Aromatic Hydrocarbon Receptor (AHR) and induces the cyto-genotoxicity. 0.1µg/ml,0.5µg/ml and 1µg/ml of UFTiO 2 when co-exposed with BaP (12 µg/ml) in A549 cells, showed significant reduction in the toxic effects of BaP, as measured by MTT Assay and ROS Assay. TiO2 showed a much higher in silico docking score with AHR (score 9920) as compared to the docking score of BaP with AHR (score 4078). The results of the study clearly indicated the toxic potential of the BaP as evident by ROS assay and MTT assay in A-549, a lung carcinoma cell line. The toxicity induced by BaP was found to reduce substantially when UF-TiO 2 was co-exposed along with BaP. The results of in silico docking fetch the insight of the mechanism how protection is provided by UF-TiO 2 against BaP induced toxicity. BaP and other PAHs are known to enter the cell via Aromatic Hydrocarbon Receptor. We observed that UF-TiO 2 binds to AHR with much more efficiency than the BaP does. This indicates a preferential binding of UF-TiO 2 with the AHR, in case if both the UF-TiO 2 and BaP are present, blocking the entry of BaP in the cell and hence protecting the cell against BaP induced toxicity.
Plenary/Invited Oral Poster Oral / Poster
ATTENUATION OF BENZO [ALPHA] PYRENE TOXICITY BY NANOPARTICLES IN A-546
CELLS
Anupam Dhasmana, Qazi Mohd. Sajid Jamal, Khurshid Ahmad, Mohtashim Lohani and Saif Khan
Department of Biotechnology, Integral University
IIT Dasauli, Kursi Road, Lucknow-226026, Uttar Pradesh, India
Email: anu_dhas_007@yahoo.co.in,qazi.bioinformatics@gmail.com
TiO2showed Human exposure to PAHs is almost inevitable. They are produced during the combustion
processes of organic materials during industrial and other human activities, like processing of coal and crude
oil, vehicle traffic and cigarette smoking. A number of PAHs like Benzo[alpha]Pyrene are known to cause
lung cancer. We investigated, if nanoparticles could somehow provide protection against the BaP induced
toxicity. In this study we evaluated the protective potential of UF-TiO2against BaP induced toxicity by Wet lab
and in silico experiments. BaP enters in the cell via Aromatic Hydrocarbon Receptor (AHR) and induces the
cyto-genotoxicity. 0.1µg/ml,0.5µg/ml and g/ml of UFTiO2when co-exposed with BaP (12 µg/ml) in A549
cells, showed significant reduction in the toxic effects of BaP, as measured by MTT Assay and ROS Assay.
TiO2 showed a much higher in silico docking score with AHR (score 9920) as compared to the docking score
of BaP with AHR (score 4078). The results of the study clearly indicated the toxic potential of the BaP as
evident by ROS assay and MTT assay in A-549, a lung carcinoma cell line. The toxicity induced by BaP was
found to reduce substantially when UF-TiO2was co-exposed along with BaP. The results of in silico docking
fetch the insight of the mechanism how protection is provided by UF-TiO2against BaP induced toxicity. BaP
and other PAHs are known to enter the cell via Aromatic Hydrocarbon Receptor. We observed that UF-TiO2
binds to AHR with much more efficiency than the BaP does. This indicates a preferential binding of UF-TiO2
with the AHR, in case if both the UF-TiO2and BaP are present, blocking the entry of BaP in the cell and hence
protecting the cell against BaP induced toxicity.
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