The aim of the study was to determine the effects of a 12-over fast bowling spell on bowling pace, accuracy and selected physiological variables. Subjects were randomly divided into an experimental (bowled 12 overs and performed physiological tests after each over) and a control group (only bowled the 1st and 12th over and performed physiological tests after each). The mean ball speed per over significantly decreased (p<0.05) from the 1st (32.9±2.1 ms-1 mean±SD) to the 12th over (32.1±1.8 ms-1) in the experimental group. This decrement in ball speed occurred after the 6th over. None of the physiological variables measured (heart rate, plasma lactate, lower and upper body muscle power and eye-hand reaction/eye-hand coordination) could account for the decrement in ball speed. Bowling accuracy did not significantly change from the 1st to the last over. A positive correlation (r=0.82; p<0.01) between eye-hand reaction score and bowling accuracy might explain the maintenance of accuracy during the trial. In conclusion, although bowling accuracy did not change, players and coaches should be aware of the possible decrement in ball speed after the 6th over, which could affect performance.