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Journal of Human Kinetics volume 45/2015, 19-26 DOI: 10.1515/hukin-2015-0003 19
Section I – Kinesiology
1 - Department of Orthopaedic Surgery, Academic Medical Center, Amsterdam, The Netherlands.
2 - MOVE Research Institute Amsterdam, Faculty of Human Movement Sciences, VU University Amsterdam, Amsterdam, The
Netherlands.
3 - Vintta | Research and Consultancy for Sport Health, Almere, The Netherlands.
4 - King Abdulaziz University, Jeddah, Saudi Arabia.
.
Authors submitted their contribution to the article to the editorial board.
Accepted for printing in the Journal of Human Kinetics vol. 45/2015 in March 2015.
Reproducibility and Validity of the Myotest for Measuring
Step Frequency and Ground Contact Time
in Recreational Runners
by
Vincent Gouttebarge1, Robin Wolfard2, Nouschka Griek2, Cornelis J. de Ruiter2,
Julitta S. Boschman3, Jaap H. van Dieën2,4
The purpose of this study was to assess the reproducibility (test-retest reliability and agreement) and
concurrent validity of the Myotest for measuring step frequency (SF) and ground contact time (GCT) in recreational
runners. Based on a within-subjects design (test and retest), SF and GCT of 14 participants (11 males, 3 females) were
measured at three different running speeds with the Myotest during two test sessions. SF and GCT were also assessed
with a foot-mounted accelerometer (Gold Standard, previously validated by comparing to force plate data) during the
first test session. Levels of test-retest reliability and concurrent validity were expressed with intraclass correlation
coefficients (ICC), agreement with standard errors of measurement (SEM). For SF, test-retest reliability (ICC’s > 0.75)
and agreement of the Myotest were considered as good at all running speeds. For GCT, test-retest reliability was found
to be moderate at a running speed of 14 km/h and poor at speeds of 10 and 12 km/h (ICC < 0.50). Agreement of the
Myotest for GCT at all three running speeds was considered not acceptable given the SEM’s calculated. Concurrent
validity of the Myotest with the foot-mounted accelerometer (Gold Standard) at all three running speeds was found to
be good for SF (ICC’s > 0.75) and moderate for GCT (0.50 < ICC’s < 0.75). The conclusion of our study is that
estimates obtained with the Myotest are reproducible and valid for SF but not for GCT.
Key words: agreement, concurrent validity, step frequency, ground contact time.
Introduction
As a consequence of its practicality and
positive effects for physical health and mental
well-being, running has in the past years become
one of the most popular forms of physical activity
(Thompson Coon et al., 2011; Williams, 2012a;
Williams, 2012b). The total number of recreational
runners has increased by 18% from 2007 to 2008 in
the United States (Running-U.S.A, 2012), while
the running population doubled within the latest
decade in the Netherlands (van Bottenburg, 2009).
Next to its beneficial health effects,
running is also associated with negative effects,
runners being at high risk of musculoskeletal
injuries (Hespanhol Junior et al., 2011; Lopes et al.,
2012). A new acute musculoskeletal injury occurs
in one out of five runners during a marathon,
with injury lasting longer than 3 months in 25% of
them (van Middelkoop et al., 2008). Known risk
factors for running injuries are diverse, among
which gender, high body mass index, history of
previous running injuries, muscle functions and
weekly training distance and frequency are the
20 Reproducibility and validity of the Myotest for measuring step frequency and ground contact time
Journal of Human Kinetics - volume 45/2015 http://www.johk.pl
most important ones (van Middelkoop et al., 2008;
Buist et al., 2010; Lopes et al., 2012; Moen et al.,
2012). Lately, running technique elements have
gained attention as risk factors for
musculoskeletal injuries. Several authors have
suggested that many running injuries might
derive from poor running technique and that
alterations in running technique elements, such as
step frequency, stride length, vertical oscillation,
ground contact time or foot strike pattern,
decrease the biomechanical load on lower
extremities, which might prevent the occurrence
of musculoskeletal injuries (Collier, 2011;
Lieberman, 2012; Rixe et al., 2012; Bochman and
Gouttebarge, 2013). Consequently, measuring and
monitoring running technique elements such as
step frequency (SF) and ground contact time
(GCT) in a practical way might be valuable for
many runners and coaches.
The Myotest Run is a practical 3D
accelerometer that has been developed as a field-
based running device meant to be used outside
such as on an athletic track by individual runners
and coaches (Myotest, 2012). The Myotest allows
to record, process, display and store data related
to running economy and performance.
Specifically, the Myotest provides data on
variables related to running technique such as SF,
stride length, vertical oscillation, GCT and
reactivity. Previous studies conducted in
laboratory setting on a treadmill have shown
some favorable findings towards the
measurement quality of this field-based running
device (high reproducibility) (Bampouras et al.,
2010; Nuzzo et al., 2011). Whether the
measurement quality of the Myotest for the
assessment of important running-related
technique aspects such as SF and GCT is also
favorable in a more practical setting such as an
athletic track remains unknown.
The measurement quality of any
instrument, test or device, specifically referring to
reproducibility and validity, needs to be explored
before its use in practice (de Vet et al., 2011). An
instrument is considered reproducible if its
measurements are consistent and stable over time
from one test moment to another (free from
significant random error), under the assumption
that the characteristic being measured does not
change over time (de Vet et al., 2011).
Reproducibility relates to two concepts, namely
reliability and agreement (de Vet et al., 2011).
Reliability refers to an instrument’s ability to
distinguish one subject from another despite
measurement errors, while agreement concerns
the absolute measurement error, evaluating how
close the scores are in repeated measurements (de
Vet et al., 2011). An instrument is considered valid
when it measures what it intends to measure (free
from significant systematic error) (de Vet et al.,
2011). Concurrent validity, an important aspect of
validity, examines at the same time how the
evaluated instrument relates to an existing, highly
valued instrument called a gold standard (shown
to be reproducible and valid) that measures the
same parameter or concept (de Vet et al., 2011).
According to the aforementioned
considerations, we aimed to explore the
measurement quality of the Myotest in terms of
reproducibility and validity, using a foot-
mounted accelerometer as gold standard as it has
been shown valid to measure SF and GCT. Our
research questions were twofold: what is the
reproducibility (test-retest reliability and
agreement) of the Myotest for measuring SF and
GCT in recreational runners and what is the
concurrent validity of the Myotest with foot-
mounted accelerometers for measuring SF and
GCT in recreational runners?
Material and Methods
Participants
Participants were healthy recreational
runners, recruited at a running association in
Amsterdam. To be eligible to be enrolled in our
study, participants were required to meet the
following inclusion criteria: (1) free from any
running-related musculoskeletal injury in the past
month, (2) being weekly active in running during
the past month, and (3) being 18 years old or
older. Sample size calculation (nQuery Advisor:
confidence interval [CI] method with a confidence
level of 0.95, correlation coefficient set at 0.90 and
limit at 0.70) indicated that at least 14 subjects
were required for this study. Consequently, 14
recreational runners (11 men, 3 women)
participated in our study. Their mean age was 45
±14 years (range, 20-68 yrs), mean height was 181
±7cm (range, 165-188 cm), and mean body weight
was 77 ±11kg (range 53-90 kg). Prior to
enrollment, and after receiving verbal and written
information on the study aim and procedures,
by Vincent Gouttebarge et al. 21
© Editorial Committee of Journal of Human Kinetics
participants signed statements of informed
consent. Subjects were free to quit the study at
any time.
Myotest
The Myotest is a small device (W x L x H:
54.2 x 102.5 x 10.7 mm, weight 59 g, sample
frequency 200-500 Hz) attached with a Velcro
waistband to the runner (Moytest, 2012). The
Myotest Runcheck software provides several
running-related parameters among which SF (in
steps per minute) and GCT (in milliseconds).
Once set up in accordance to a runner’s
characteristics (sex, height, weight and level of
expertise), the device was attached to the Velcro
waistband around the runner’s iliac crest, on the
ventral side of the body. This standardized
position allows the runner to keep their full range
of motion. Then, the runner only had to press the
enter button in the middle of the device to start
the data collection. The same enter button needed
to be pressed to stop the device. For our study, the
level of expertise was set to “Expert” for all
subjects.
Gold standard
Foot-mounted accelerometer was used in
our study as gold standard, as it has been
developed and validated to measure SF and GCT
(de Ruiter et al., 2013). Containing a tri-axial
accelerometer (+6 g; 1000 Hz, MMA7361L,
Freescale Semiconductor, Austin, Texas, USA), the
foot-mounted accelerometer uses a software
algorithm (MATLAB R2010a, Mathworks, Natick,
USA) based on the open-source platform
Arduino. A foot-mounted accelerometer was
attached at each shoe of the participant by using
the shoe lace, sports tape being also used to secure
its sustainable position (Figure 1). Transmitting
data wirelessly, SF (in steps per minute) and GCT
(in milliseconds) were calculated automatically
and exported directly to a Microsoft Excel file.
Procedures
An experimental study using a within-
subjects design (test-retest) was conducted to
assess reproducibility and concurrent validity of
the Myotest. Each participant was assessed during
two test sessions, using a time interval of 7 ±4
days between both test days. We assumed that
such a time interval was optimal to assure a
steady state in participants. In addition,
participants were asked to wear the same running
shoes during both test sessions. Participants were
asked before each test session to avoid any
training session and exhaustive event in the
previous 24 and 36 hours, respectively. Prior to
each test session, measurement devices (Myotest
and foot-mounted accelerometer) were attached
to the participants by the same researcher (NG)
and set up in order to be ready for measurement.
Before each test session, participants were
informed one more time about the experimental
procedures in order to prevent misunderstanding,
and were asked to perform a standardized warm-
up (jogging at a comfortable pace without fatigue
development). A test session consisted of three
runs of 400 meters on an outdoor athletic track:
the first run at an approximated speed of 10 km/h,
the second run at an approximated speed of 12
km/h, and the third run at an approximated speed
of 14 km/h (approximated speed fed back verbally
every 100 m). Between the three runs, participants
were allowed to rest as required but up to 2
minutes. During the first test session, SF and GCT
were assessed concurrently by the Myotest and
the foot-mounted accelerometer (gold standard).
During the second test session, SF and GCT were
measured only by the Myotest. Ethical approval
was not needed from the ethical committee of the
Faculty of Human Movement Sciences of the VU
University as the study did not fall into the
Medical Research Involving Human Subjects Act.
The study was carried out in accordance with the
Declaration of Helsinki (2000).
Statistical Analysis
Means, standard deviations (SD’s), and
ranges were calculated for each outcome measure
at each test session. Reliability and agreement
were determined using the SF and GCT outcomes
assessed by the Myotest during the two test
sessions (test and retest). The level of test-retest
reliability was expressed with the intraclass
correlation coefficient (ICC; two-way random
model, agreement, single measures) and its 95%
confidence interval (95% CI) (Portney and
Watkins, 2008; de Vet et al., 2011). Agreement was
expressed with the standard error of
measurement (SEM = √ [var(raters) + var(error)] or
SEM = SD x √[1 – ICC]). Concurrent validity was
investigated by comparing the SF and GCT
outcomes assessed by the Myotest to the SF and
GCT outcomes assessed by the foot-mounted
accelerometer (gold standard) (Portney and
22 Reproducibility and validity of the Myotest for measuring step frequency and ground contact time
Journal of Human Kinetics - volume 45/2015 http://www.johk.pl
Watkins, 2008; de Vet et al., 2011). The level of
concurrent validity was expressed with the
intraclass correlation coefficient (ICC; two-way
random model, consistency, single measures) and
its 95% confidence interval (95% CI) (Portney and
Watkins, 2008; de Vet et al., 2011). ICC’s obtained
for reliability and concurrent validity were
interpreted as good for ICC > 0.75, as moderate
for 0.50 ≤ ICC ≤ 0.75, and as poor for ICC < 0.50
(Portney and Watkins, 2008; de Vet et al., 2011).
All data analyses were performed using the
statistical software IBM SPSS Statistics 22.0 for
Windows.
Results
Reliability and agreement
Table 1 presents the averages, SD’s, and
ranges of the SF and GCT measured with the
Myotest at different speeds during both test
sessions (test and retest), and their related ICC’s
(95% confidence interval) and SEM’s. The level of
test-retest reliability of the Myotest for SF was
good at all three running speeds, with ICC’s
ranging from 0.78 to 0.92. The SEM of the Myotest
for SF, expressed in steps per minute, were rather
small given the mean values found during both
test sessions. For instance, mean SF measured by
the Myotest at 14 km/h was 175-176 steps per
minute and its SEM 3 steps per minute, indicating
that an increase or decrease of 6 steps per minute
cannot be interpreted as a random measurement
error. The level of test-retest reliability of the
Myotest for GCT was poor to moderate at
different running speeds as ICC’s ranged from -
0.24 to 0.67. The SEM of the Myotest for GCT,
expressed in ms, were not acceptable, being large
relative to the mean values found during both test
sessions. For instance, mean GCT measured by
the Myotest at 12 km/h was 156-159 ms and its
SEM 15 ms, indicating that an increase or decrease
of more than 30 ms needs to be reached before
one can interpret it as more than a random
measurement error.
Concurrent validity
Table 2 presents the averages, SD’s, and ranges
of the SF and GCT measured with the Myotest
and foot-mounted accelerometer (gold standard)
at different speeds during the first test session,
and their related ICC’s (95% confidence interval).
The level of concurrent validity of the Myotest
with the foot-mounted accelerometer (gold
standard) for SF was good at all three running
speeds as ICC’s ranged from 0.78 to 0.90. The
level of concurrent validity of the Myotest with
the foot-mounted accelerometer (gold standard)
for GCT was only moderate at all three running
speeds as ICC’s ranged from 0.48 to 0.50.
Table 1
Mean scores, standard deviation and range obtained from the Myotest for step frequency
(SF; step per minute) and ground contact time (GCT; ms)
at different running speed, and the level of test-retest reliability and agreement of the Myotest
Test session 1 Test session 2 ICC (95% CI) SEM
Mean SD Range Mean SD Range
SF at 10 km/h 164.3 7 155-181 164.4 9 153-183 0.82 0.52-0.94 3.5
SF at 12 km/h 168.9 8 161-185 169.4 10 161-193 0.78 0.44-0.92 4.1
SF at 14 km/h 175.9 10 163-199 176.9 10 164-203 0.92 0.77-0.97 3.0
GCT at 10 km/h 172.0 15 154-204 165.4 17 113-188 -0.24 -0.69-0.32 n/a
GCT at 12 km/h 159.1 17 123-194 156.4 20 106-189 0.35 -0.23-0.74 14.8
GCT at 14 km/h 144.2 16 103-177 142.9 18 102-176 0.67 0.22-0.88 10.1
SD, standard deviation; ICC, Intra-Class correlation coefficient;
CI, confidence interval; SEM, standard error of measurement; n/a, not applicable
by Vincent Gouttebarge et al. 23
© Editorial Committee of Journal of Human Kinetics
Table 2
Mean scores, standard deviation and range obtained from the Myotest
and foot-mounted accelerometer (Gold Standard) for step frequency
(SF; step per minute) and ground contact time (GCT; ms)
at different running speed, and the level of concurrent validity
Myotest foot-mounted accelerometer ICC (95% CI)
Mean SD Range Mean SD Range
SF at 10 km/h 164.3 7 155-181 165.6 8 156-183 0.89 0.69-0.96
SF at 12 km/h 168.9 8 161-185 169.4 8 161-184 0.78 0.45-0.96
SF at 14 km/h 175.9 10 163-199 175.7 13 157-198 0.90 0.72-0.97
GCT at 10 km/h 172.0 15 154-204 297.1 20 256-331 0.49 -0.03-0.80
GCT at 12 km/h 159.1 17 123-194 278.4 25 241-314 0.50 -0.02-0.81
GCT at 14 km/h 144.2 16 103-177 251.3 24 205-274 0.48 -0.07-0.81
SD, standard deviation; ICC, Intra-Class correlation coefficient; CI,
confidence interval; n/a, not applicable
Figure 1
Foot-mounted accelerometer (Gold Standard) attached to the runner’s shoe
24 Reproducibility and validity of the Myotest for measuring step frequency and ground contact time
Journal of Human Kinetics - volume 45/2015 http://www.johk.pl
Discussion
Using a within-subjects design on runners
free from musculoskeletal injuries, the purpose of
our study was to evaluate the reliability,
agreement and concurrent validity of the Myotest
for measuring SF and GCT at three different
running speeds. For SF, test-retest reliability and
agreement of the Myotest were evaluated as good
at all running speeds. For GCT, test-retest
reliability was found to be moderate at a running
speed of 14 km/h and poor at speeds of 10 and 12
km/h. Agreement of the Myotest for GCT at all
three running speeds was not acceptable given the
large SEM. Concurrent validity of the Myotest
with the foot-mounted accelerometer (gold
standard) at all three running speeds was found
to be good for SF and moderate for GCT.
Empirical studies assessing the
measurement quality of the Myotest are scarce,
especially related to the assessment of running
parameters. In a previous study, reliability,
agreement and validity of the Myotest for
measuring running economy and vertical
oscillation were explored among healthy runners
(Potter et al., submitted). For both running
economy and vertical oscillation, levels of (test-
retest) reliability were moderate to good (ICC >
0.50) while SEM (agreement) were acceptable
relative to the mean values (Potter et al.,
submitted). In contrast, the validity of the Myotest
for measuring running economy and vertical
oscillation was only poor to moderate (Potter et
al., submitted). For measuring other performance
parameters such as force (countermovement
vertical jump), the Myotest was found to be
highly reliable (test-retest reliability) and valid,
endorsing its application in the field for the
assessment of force related parameters
(Bampouras et al., 2010; Nuzzo et al., 2011). Other
accelerometers similar to the Myotest have also
shown some moderate to high evidence of test-
retest reliability and validity (Thompson and
Bemben, 1999; Brage et al., 2003; Esliger et al.,
2006). However, most of these studies were
conducted in laboratory setting and not
specifically related to running parameters.
Our study was conducted in a practical
context, which can be seen as a strength of our
experiment. The Myotest is a small and practical
3D accelerometer that has been developed as a
field-based running device meant to be used
outside such as on an athletic track by individual
runners and coaches (Myotest, 2012).
Consequently, even though the use of a treadmill
might offer safer and more controlled conditions,
the measurement quality of the Myotest should in
principle be determined for measurements in the
field such as on an athletic track. A gait on a
treadmill has been shown to differ from a gait on
an outside track, endorsing the choice for our
experimental conditions (Kirtley, 2006). When it
comes to criterion-related validity (concurrent and
predictive), the availability of a gold standard, an
instrument already known as reliable and valid
measuring the same construct, is crucial (de Vet et
al., 2011). In our study, we chose a foot-mounted
accelerometer, as it has been validated for SF and
GCT by comparing it to a force platform (de
Ruiter and al., 2013).
With regard to our findings, the Myotest
was found to be reproducible and valid for
measuring SF at 10, 12 and 14 km/h. It has been
shown that SF between 180 and 185 steps per
minute significantly decreases biomechanical
loads on the lower extremities (peak vertical
ground reaction force, energy generated by the
hip, knee and ankle joints, ground reaction force
and compartment pressures), compared to lower
SF usually preferred by novice runners
(Boschman and Gouttebarge, 2013). Also running
economy can be improved in novice runners by
adopting a higher SF (de Ruiter et al., 2013).
Consequently, runners (or their coaches) could
use the Myotest to get feedback about their
current SF, striving to learn to run at higher SF
and using the Myotest to monitor this process
over time. By contrast to SF, since no instrument
can be valid if not reliable (de Vet et al., 2011), the
Myotest was not reliable nor valid for measuring
GCT and thus cannot be used for measuring GCT.
In addition, the GCTs found with the myotest
(Table 2) are too low when compared with values
(250-350 ms) reported in the literature at these
speeds (de Ruiter et al., 2013).
In conclusion, our study results suggest
that the Myotest is a practical, useful, reliable and
valid device that can be used by runners and
coaches to assess and monitor SF outside on an
athletic track. For measuring GCT, the Myotest
should not be used yet as it was not sufficiently
reliable nor valid. Future research focusing on the
Myotests’s reproducibility and validity for
by Vincent Gouttebarge et al. 25
© Editorial Committee of Journal of Human Kinetics
measuring other running technique elements,
such as vertical oscillation, stride length or foot
strike pattern, when running on an athletic track
is needed. In addition, attention should also be
given to responsiveness when change in running
technique elements over time is aimed for.
Acknowledgements
This study received no specific grant from any funding agency in the public, commercial or not-for-
profit sectors. The authors would like to thank the recreational runners for their participation in the study.
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Corresponding author:
Dr. Vincent Gouttebarge
Department of Orthopaedic Surgery, Academic Medical Center, Amsterdam, The Netherlands
Phone: +31621547499
E-Mail: v.gouttebarge@amc.nl