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Effects of black seeds (Nigella sativa) on male infertility: A systematic review
Abstract
Following an increase in free radicals, DNA damage and lipid peroxidation in human sperm may occur. Antioxidant components in medicinal herbs such as Nigella sativa (NS) have been indicated to improve spermatogenesis and steroidogenesis. The aim of the present study was to conduct a systemic review summarizing the effects of NS on male infertility. A number of electronic databases were searched namely Pubmed, Science Direct, Google scholar and Springer from the period January 2000 until June 2014. Searching was limited to articles in the English language. Patents and abstracts from symposiums and congress were excluded because they contained insufficient information for evaluation and comparison with other studies. The outcome of this study indicated that NS can positively influence sperm parameters, semen, Leydig cells, reproductive organs and sexual hormones. The main potential mechanism is through the antioxidant properties of NS. Thymoquinone (TQ) and unsaturated fatty acids are the main antioxidant components of NS. NS and TQ derived from NS can improve male fertility parameters through promoting antioxidant defence. Although the findings of this review suggest that NS is a good candidate for male infertility treatment, there is insufficient evidence to make recommendations for its use as an adjunct therapy in infertile men. More clinical trials are recommended to demonstrate the efficacy of NS on male infertility.
... Additionally, it is clear that the black seeds are an effective treatment for those with dyslipidemia and the insulin resistance syndrome. Diabetes patient Meriones Shawi treated with N. sativa likewise had an insulin-sensitization effect through increased ACC phosphorylation (primarily MAPK signalling pathway) and muscle GLUT4 content as well as gradual restoration of glycemia [1,28]. In rats with diabetic mellitus (DM) brought on by streptozotocin, lipid and volatile fractions decreased toxicological and unfavourable effects [29]. ...
... Treatment of 34 infertile men for two months with 2.5 mL black seed oil enhanced their abnormal semen quality without having any negative effects [39]. Black seed oil is a promising therapy for treating male infertility, according to Mahdavi et al. [28] In Sprague-Dawley male and female rats, N. sativa extracts in hexane and methanol significantly reduced fertility. In contrast, N. sativa prevented the contraction of uterine smooth muscle in rats and guinea pigs [1,28]. ...
... Black seed oil is a promising therapy for treating male infertility, according to Mahdavi et al. [28] In Sprague-Dawley male and female rats, N. sativa extracts in hexane and methanol significantly reduced fertility. In contrast, N. sativa prevented the contraction of uterine smooth muscle in rats and guinea pigs [1,28]. TQ reduced the number of polycystic ovaries in rats by reducing their exposure to olive oil [40]. ...
Nigella sativa oils have anti-inflammatory, antibacterial, antifungal, antiparasitic and antiprotozoal, antiviral, cytotoxic, anticancer, neuro-, gastro-, cardio-, and hepatoprotective properties, making them potential treatments for a wide range of illnesses. N. sativa oil also suggests positive benefits on the immunological, respiratory, and reproductive systems in addition to diabetes mellitus (DM), fertility, breast cancer, dyspepsia, osmotic balance, and other conditions. Thymoquinone (TQ) is a suitable target for its potential antibacterial, antimicrobial, anti-inflammatory, chemopreventive, antitumoral, and other actions among the various isolated chemical moieties. The N. sativa oil has been shown in various non-clinical and clinical investigations to benefit health. On the other hand, TQ in several animal experiments is clear to generate no adverse modifications of the body biomarkers; rather, it enhanced health quality. This study presents a more mechanistic review of the constitutions and oil of N. sativa. In conclusion, research on Nigella oil may represent a health breakthrough.
... The main phytotherapics promoted to increase T and improve sperm characteristics in humans are: tribulus (Tribulus terrestris L., Zygophyllaceae), maca (Lepidium meyenii Walp., Brassicaceae), long Jack (Eurycoma longifolia Jack, Simaroubaceae), mucuna (Mucuna pruriens (L.) DC, Fabaceae), ashwagandha (Withania somnifera L. Dunal, Solanaceae) fenugreek (Trigonella foenum-graecum L., Fabaceae) and black seeds (Nigella sativa L., Ranunculaceae) (Bhat and Karim, 2010;Chen et al., 2014;Mahdavi et al., 2015;Khanijo and Jiraungkoorskul, 2016;Neychev and Mitev, 2016;Rehman et al., 2016;Talbott et al., 2013). Previous reviews have overestimated the effectiveness of these phytotherapics to favorably influence hormonal parameters, and, to date, the real physiological impact remains unclear (Chhatre et al., 2014;Malviya et al., 2011;Nagulapalli Venkata et al., 2017;Yadav and Baquer, 2014;Zhu et al., 2017). ...
... months (Ahmad et al., 2008;Mahdi et al., 2011;Shukla et al., 2010Shukla et al., , 2010Shukla et al., , 2009). Black seeds, in turn, also display an important role on seminal redox balance, where its antioxidants compounds, especially thymoquinone and unsaturated fatty acids, have been suggested to improve spermatogenesis through free radical scavenging potential (Mahdavi et al., 2015). ...
... Black seeds positively influence sperm parameters, Leydig cells, reproductive organs, and sexual hormones. However, the current body of evidence for black seeds is based on animal studies as detailed in a 2015 systematic review (Mahdavi et al., 2015). Hence, there is insufficient evidence to make recommendations on the use of black seeds as an adjunct therapy for infertility. ...
Ethno-pharmacological relevance:
Phytotherapeutic approaches have been widely proposed to improve male health. Despite the well-touted effects of tribulus (Tribulus terrestris L) on men's health, an optimal phytotherapy remains an elusive challenge.
Aim of the review:
We sought to critically analyze the evidence in the phytotherapic literature beyond the effects of tribulus on testosterone (T) concentration and sperm analysis to also include indications for prostate health.
Materials and methods:
A focused literature search was conducted to include studies published in Cochrane, Pubmed, and Web of Science databases between the years 2002 and 2018.
Results:
The use of tribulus and maca (Lepidium meyenii Walp, Brassicaceae) were not scientifically supported to improve serum T levels in men. Moderate evidence supports the use of long Jack (Eurycoma longifolia Jack, Simaroubaceae), mucuna (Mucuna pruriens (L.) DC., Fabaceae), ashwagandha (Withania somnifera (L.) Dunal, Solanaceae), fenugreek (Trigonella foenum-graceum L., Fabaceae), and black seeds (Nigella sativa L., Ranunculaceae) to increase total T and improve seminal parameters. Data suggests an increase in total T with the use of 5000 mg/d of powdered mucuna seed and ashwagandha root (151 and 143 ng/dL, respectively) over a 12-week period in patients with oligozoospermia. The use of mucuna was supported for patients with oligozoospermia to improve sperm parameters, with an increase of 83.3 million/mL observed after use of 5000 mg/d of powdered mucuna seed over a 12-week period. Evidence supporting the use of saw palmetto (Serenoa repens, (W.Bartram) Small, Arecaceae) to improve prostate health remains equivocal; whereas, evidence supporting the use of Pygeum africanum Hook.f., Rosaceae, Urtica dioica L., Urticaceae, beta-sitosterols, pollen extract, onion, garlic, and tomato, appears favorable and promising.
Conclusion:
Scientific evidence supports the use of mucuna and ashwagandha as phytotherapics for improving serum T concentrations and semen parameters. Despite inconclusive evidence for use of tribulus as a T booster, it may provide advantageous effects on sperm parameters in men with idiopathic infertility. Nutraceutical strategies and some phytotherapics may also be effective to promote prostate health. Popular foodstuffs (onion, garlic, and tomato), nutraceutical agents (pollen extract and beta-sitosterols), and herbal medicines (Pygeum africanum and Urtica dioica) are rational approaches.
... A few of them covered co-treatments with other agents including biochemicals. We found a major revision on this plant done by Ahmad et al. [2] along with a dermatological revision of Aljabre et al. [3], an immunomodulatory and antiinflammatory revision of Amin et al. [4], an anti-inflammatory, antioxidant, an immunomodulatory revision of Gholamnezhad et al. [5], male fertility revision of Mahdavi et al. [6], and metabolic parameters in diabetes mellitus revision of Heshmati & Namazi [7]. These six articles inspired me to take them as a guide for previous evidences on N. sativa. ...
... The black seeds are also evident an advantageous therapy in insulin resistance syndrome and dislipidemic patients. An insulin-sensitization action via enhancing ACC phosphorylation (mainly MAPK signaling pathway) and muscle GLUT 4 content as well as progressive normalization of glycaemia are also seen in N. sativa treated diabetic Meriones shawi [2,6]. Lipid (4%) and volatile (3%) fractions in streptozotocin-induced diabetes mellitus (DM) rats reduced toxicological and adverse consequences to the animals [16]. ...
... Infertile men (n=34) when treated with 2.5mL black seed oil for 2 months improved abnormal semen quality without producing any adverse effect was observed [33]. According to Mahdavi et al. [6] the black seed oil is a good candidate for treating male infertility. Hexane and methanol extracts of N. sativa produced significant anti-fertility in Sprague-Dawley male and female rats, respectively. ...
Nigella sativa and its constitutions including some isolated compounds are the potential sources of remedies of varieties of ailments such as antioxidant, anti-inflammatory, antibacterial, antifungal, antiparasitic and antiprotozoal, antiviral, cytotoxic, anticancer, neuro-, gastro-, cardio-, hetapto- and nephroprotective activities. In addition, the N. sativa implies beneficiary effects on reproductive, pulmonary and immune systems along with diabetes mellitus (DM), fertility, breast cancer, dermatological complications, dehydration, dyspepsia, osmotic balance and so on. Among the other isolated chemical moieties, thymoquinone (TQ) is a good target for its potential antimicrobial, antimicrobial, anti-inflammatory, chemopreventive, antitumoral and other activities. The N. sativa is evident to promote health in some non-clinical and clinical studies. Otherwise, TQ in a number of animal test systems is evident to produce no negative alterations of the body biomarkers in contrary it improved health quality. This paper depicts a more mechanistic revision on N. sativa and its constitutions. In conclusion, findings on Nigella may be featured as a health jackpot.
... Moreover, infertile men (n = 34) when treated with 2.5 mL NS oil for 2 months, significantly improved abnormal semen quality without producing any adverse effect [35]. According to Mahdavi et al [36] the NS oil is a good candidate for treating male infertility. Hexane and methanol extracts of NS produced significant anti-fertility in Sprague-Dawley male and female rats, respectively. ...
... Hexane and methanol extracts of NS produced significant anti-fertility in Sprague-Dawley male and female rats, respectively. Otherwise, NS is evident to inhibit the uterine smooth muscle contraction in rats and guinea pigs [2,36]. TQ when treated with olive oil caused a reduction in polycystic overy in rats possibly, via NF-κB signaling pathway [37]. ...
... NS seed oil -Male infertility treatment. [36] NS seed oil -Anti-inflammatory, antioxidant, and immunomodulatory activities. [14] EO from NS and TQ -Regulation of immune reactions implicated in various infectious and non-infectious conditions including different types of allergy, autoimmunity, and cancer. ...
Nigella sativa L. (NS) has a number of important biological activities, such as antioxidant, anti-inflammatory, antibacterial, antifungal, anti-viral, anti-parasitic and anti-protozoal, cytotoxic, anticancer, neuro-, gastro-, cardio-, hetapto- and nephroprotective activities. In addition, the NS exerts beneficiary effects on reproductive, pulmonary and immune systems as well as in diabetes mellitus (DM), fertility (male and female), breast cancer, dermatological complications, dehydration, dyspepsia, and osmotic balance and so on. Among the isolated constituents, thymoquinone (TQ) is a vastly studied phytochemical in NS. A number of reports suggest that, the NS may be one of the potential herbs in health promotion. This review paper will discuss the detailed and mechanistic aspects of the biological activity of N. sativa along with the reported phytoconstituents.
... Inability to conceive after one year of unprotected sexual intercourse (infertility) results in low birth rate. Infertility is a major global issue which impacts negatively on patients and their family members [1]. In China, the prevalence of infertility among newly married couples ranges from 8 -13.6 % [2]. ...
... Sperm cell membrane is rich in polyunsaturated fatty acids (PUFAs), phospholipids (PLs) and mitochondria, making it highly susceptible to oxidative stress-induced damage. Oxidative stress results in high levels of lipid peroxidation product (MDA) and DNA-damage product (8-OHdG) [1,3]. Cell apoptosis, reduction in levels of endogenous antioxidants and increased production of lipid peroxidation and DNA damage products contribute to oxidative stress in cryptorchidism rats, and ultimately to infertility [9,20,24]. ...
Purpose: To investigate the fertility-enhancing potential of the ethanol extract of Cuscuta chinensis seeds in a rat model of unilateral cryptorchidism (ULC), and the mechanism(s) of action. Methods: Healthy male Sprague Dawley rats (n = 48; mean weight = 220 ± 10 g) were randomly assigned to 4 groups (12 rats/group): control, ULC, 100 mg extract/kg and 200 mg extract/kg groups. Unilateral cryptorchidism of right testis was induced via standard method using an operating microscope. Rats in the treatment groups received 100 and 200 mg of ethanol extract of Cuscuta chinensis/kg orally once a day for 60 days. Sperm count and sperm motility were determined in seminal vesicular fluid (SVF) suspension. Oxidative stress markers and histological changes in rat testis were evaluated. The levels of caspase-3 and caspase-9 in testicular tissue were assessed by enzyme-linked immunosorbent assay (ELISA), while the protein expressions of Nrf2 and heme oxygenase 1 (HO-1) were determined using Western blotting. Results: Body and reproductive organ weights, sperm count, sperm motility, and activities of glutathione peroxidase (GPx), catalase and superoxide dismutase (SOD) were significantly reduced in ULC group, relative to control group, but these parameters were significantly and dose-dependently increased following extract treatment (p < 0.05). Malondialdehyde (MDA), 8-hydroxy-2’-deoxyguanosine (8-OHdG), caspase-3 and caspase-9 levels were significantly higher in ULC group than in control group, but they were reduced significantly and dose-dependently after extract treatment (p < 0.05). Moreover, the protein expressions of Nrf2 and HO-1 were significantly downregulated in ULC group, when compared with control group, but they were significantly and dose-dependently upregulated by the extract (p < 0.05). Cross sections of testicular tissues of rats in ULC group revealed narrowed and thickened seminiferous tubules (disrupted spermatogonia) characterized by increased apoptotic bodies (increased number of necrotic Sertoli and Leydig cells). However, there were few damaged or necrotic Sertoli and Leydig cells, and complete absence of thickening of seminiferous tubules in testicular tissues of rats treated with the extract. Conclusion: The ethanol extract of Cuscuta chinensis seeds effectively mitigates cryptorchidism in rats via mechanisms involving the regulation of Nrf2/HO-1 signaling pathway, and inhibition of apoptosis and oxidative stress. Thus, the plant extract has potentials for further development for the management of male infertility
... Furthermore, the optimal doses of NSO decreased the deleterious effects of lead on reproductive organs. The exposure of normal rats to NSO showed a rise in sperm production and testosterone levels, indicating that these seeds boost male fertility (Mahdavi et al., 2015). This study observed that exposure to NSO with PbO for 60 days led to a reduction in the toxicity of PbO on the gonadal gland, sex hormones, and spermatogenesis. ...
... These findings agree with the remarks that show PbO acts as a spermicidal factor, leading to the suppression of serum reproductive hormone concentration and spermatogenesis in rats. Oral treatment of antioxidant NSO has partly alleviated the effects of lead and induced histological changes in the testis but has not completely prevented them (Mahdavi et al., 2015;Assi et al., 2016). ...
The prevalence of testicular toxicity induced via contamination with heavy metals is increasing worldwide, and a close connection between testicular toxicity and lead has recently been identified. The present study demonstrated the possible therapeutic and protection properties of Nigella sativa oil (NSO) against dangerous testicular toxicity and sexual behaviour disorders in rat models induced by lead monoxide (PbO) food poisoning. A total of 24 male rats were used and divided into four groups, consisting of Group 1: Control (1 mL of distilled water); Group 2: PbO (80 mg/kg); Group 3: NSO (0.5 mL/rat); and Group 4: (NSO+PbO) 0.5 mL/rat of NSO, followed by PbO (80 mg/kg/day) respectively for 60 days. The testosterone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), 17 beta-hydroxysteroid dehydrogenase activity (17β-HSD), sperm parameters, and sexual behaviour (copulation behaviour test) were measured. The results showed that sex hormone levels were significantly decreased (p<0.05) in rats exposed to PbO, while the levels of these hormones were significantly higher in rats exposed to NSO and NSO+PbO. The sperm parameters (numbers, concentrations, motilities, and abnormality) were lower in rats exposed to PbO compared to those in the control, NSO, and NSO+PbO groups that showed significant increases. Sperm abnormality and 17β-HSD activity showed a significant increase at p<0.05 in the group exposed to the PbO compared to those of the control, NSO, and NSO+PbO groups. The results also significantly suppressed sexual behaviour in rats exposed to PbO compared to those in the control, NSO, and NSO+PbO groups, rats were administered NSO+PbO showed improvement in sexual behaviour parameters compared to PbO groups. PbO toxicity was obvious in the reproductive tract through severe histopathological changes. The impact of lead on tissues was partially mitigated by NSO. The study showed protective effects of N. sativa oil against changes in sex hormones, sperm characteristics, and sexual behaviour in male rats exposed to lead monoxide-induced testicular toxicity.
... Poultry spermatozoon is composed of polyunsaturated fatty acids. The high content of polyunsaturated fatty acids triggers the oxidative stress which can damage the function, viability, and structure of sperm (Mahdavi et al., 2015). Substances with high antioxidant content can inhibit the production of free radicals and, by doing so, prevent the damage to sperm. ...
... Substances with high antioxidant content can inhibit the production of free radicals and, by doing so, prevent the damage to sperm. Mahdavi et al. (2015) reported that antioxidant components could improve spermatogenesis and steroidogenesis. Some previous studies found that the antioxidant components in the structure of medicinal and aromatic plants could eliminate free radicals and prevent the harmful consequences of oxidative stress. ...
The purpose of this study was to examine the effect of adding different levels of black cumin seed (BCS) to the compound feed on performance, serum criteria, and reproductive hormones in male Japanese quails (Coturnix coturnix Japonica). 80 male Japanese quails (Coturnix coturnix Japonica) at the age of 21 days were used in the study. The trial was carried out with 5 repetitions in 4 treatment groups (20 subgroups with 4 animals in each cage) and completed in 3 weeks. The treatment groups were as follows: Group A (the control group fed with basal feed, 0%); Group B (the group fed with basal feed + 0.5% black cumin seed); Group C (the group fed with basal feed + 1.0 % black cumin seed); and Group D (the group fed with basal feed + 2.0% black cumin seed). In the study, it was found that the addition of black cumin seed to the rations of male quails had no significant effect on their performance, testicular characteristics, serum parameters, and reproductive hormones. However, it was also found that the addition of 0.5% BCS caused an increase in the ratio of testicular weight to live weight, and the serum FSH levels tended to decrease with the addition of BCS; so there is a need for more studies on this subject.
... In another study examining the effect of obesity on testicular tissue and sperm parameters, it was reported that TQ protects the testis from the harmful effects of obesity by increasing the healthy sperm count and decreasing sperm anomalies (Tufek et al., 2015). In addition, Mahdavi et al. (2015) reported that TQ can positively affect sperm parameters, semen quality, Leydig cells, reproductive organs and sexual hormones, and these effects of TQ may be due to its antioxidant properties (Mahdavi et al., 2015). ...
... In another study examining the effect of obesity on testicular tissue and sperm parameters, it was reported that TQ protects the testis from the harmful effects of obesity by increasing the healthy sperm count and decreasing sperm anomalies (Tufek et al., 2015). In addition, Mahdavi et al. (2015) reported that TQ can positively affect sperm parameters, semen quality, Leydig cells, reproductive organs and sexual hormones, and these effects of TQ may be due to its antioxidant properties (Mahdavi et al., 2015). ...
The aim of this study was to investigate the protective and therapeutic effects of thymoquinone against the negative effects of varicocele on testicular tissue and sperm morphology. Five groups were formed by random selection from a total of 40 adult male Wistar rats (n = 8). Thymoquinone (5 mg/kg/day) was administered intraperitoneally to the varicocele‐dimethyl sulfoxide‐olive oil‐thymoquinone (VT) group and the sham‐thymoquinone group. At the end of the 60th day, all groups were anaesthetised and the left testis was removed from the body quickly. One half of the testis tissue, which was divided into two, was separated for biochemical and Western blot analysis, while the other half were fixed in Bouin's fixative. As a result of biochemical, molecular and histopathological analyses, a statistically significant increase was found in the varicocele group testicular tissues in the malondialdehyde level, apoptotic index, Bax expression, cytochrome c expression and Bax/Bcl‐2 ratio compared with the sham group. In addition, histopathological changes characterised by partial or complete degeneration of the germinal epithelium were observed in the seminiferous tubules in the same group. Total oxidant status level and sperm count with abnormal morphology increased in varicocele group, whereas total antioxidant status level decreased. In the VT group, all of the biochemical, molecular and histopathological changes detected in the varicocele group were statistically significantly reduced. When the findings obtained in this study are evaluated, it can be said that thymoquinone has the potential to be used as a preventive and therapeutic pharmacological agent in the medical treatment of varicocele. Although the exact mechanism of action of thymoquinone has not been fully elucidated, the findings obtained in this study support the view that thymoquinone showed a cytoprotective effect by reducing apoptosis, oxidative stress and lipid peroxidation.
... Consequentially this leads to protect sperm and semen against testicular toxins. (Hala, 2011;Mahdavi et al., 2015). ...
... The anti-inflammatory properties were widely searched in two major inflammatory disorders that include allergic encephalomyelitis and ulcerative colitis (Majdalawieh and Fayyad, 2015;Nieto et al., 2000). It has been found that TQ through anti-inflammatory and antioxidant properties significantly improve allergic encephalomyelitis and ulcerative colitis (Choudhary et al., 2001;Koch et al., 2000;Mahdavi et al., 2015). ...
N. sativa (N. sativa) has been used since ancient times, when a scientific concept about the use of medicinal plants for the treatment of human illnesses and alleviation of their sufferings was yet to be developed. It has a strong religious significance as it is mentioned in the religious books of Islam and Christianity. In addition to its historical and religious significance, it is also mentioned in ancient medicine. It is widely used in traditional systems of medicine for a number of diseases including asthma, fever, bronchitis, cough, chest congestion, dizziness, paralysis, chronic headache, back pain and inflammation. The importance of this plant led the scientific community to carry out extensive phytochemical and biological investigations on N. sativa. Pharmacological studies on N. sativa have confirmed its antidiabetic, antitussive, anticancer, antioxidant, hepatoprotective, neuro-protective, gastroprotective, immunomodulator, analgesic, antimicrobial, anti-inflammatory, spasmolytic, and bronchodilator activity. The present review is an effort to explore the reported chemical composition and pharmacological activity of this plant. It will help as a reference for scientists, researchers, and other health professionals who are working with this plant and who need up to date knowledge about it.
... Consequentially this leads to protect sperm and semen against testicular toxins. (Hala, 2011;Mahdavi et al., 2015). ...
... The anti-inflammatory properties were widely searched in two major inflammatory disorders that include allergic encephalomyelitis and ulcerative colitis (Majdalawieh and Fayyad, 2015;Nieto et al., 2000). It has been found that TQ through anti-inflammatory and antioxidant properties significantly improve allergic encephalomyelitis and ulcerative colitis (Choudhary et al., 2001;Koch et al., 2000;Mahdavi et al., 2015). ...
Nigella sativa (N. sativa) has been used since ancient times, when a scientific concept about the use of medicinal plants for the treatment of human illnesses and alleviation of their sufferings was yet to be developed. It has a strong religious significance as it is mentioned in the religious books of Islam and Christianity. In addition to its historical and religious significance, it is also mentioned in ancient medicine. It is widely used in traditional systems of medicine for a number of diseases including asthma, fever, bronchitis, cough, chest congestion, dizziness, paralysis, chronic headache, back pain and inflammation. The importance of this plant led the scientific community to carry out extensive phytochemical and biological investigations on N. sativa. Pharmacological studies on N. sativa have confirmed its antidiabetic, antitussive, anticancer, antioxidant, hepatoprotective, neuro-protective, gastroprotective, immunomodulator, analgesic, antimicrobial, anti-inflammatory, spasmolytic, and bronchodilator activity. The present review is an effort to explore the reported chemical composition and pharmacological activity of this plant. It will help as a reference for scientists, researchers, and other health professionals who are working with this plant and who need up to date knowledge about it
... In the present study, improvement of both the prostate gland and seminal vesicle histological features is possibly due to the antioxidant properties of N. sativa that could neutralize ROS by enhancing the scavenging system. Result of the present study was in agreement to that reported by Reza et al. (2015). The authors observed positive influence of N. sativa on sperm parameters, semen, Leydig cells, reproductive organs and sexual hormones. ...
... According to the authors, the main potential mechanism is through the antioxidant properties of N. sativa. The main antioxidant component of N. sativa is thymoquinone (TQ) that can improve male fertility parameters through promoting antioxidant defence (Reza et al. 2015). ...
Nicotine is claimed to increase free radicals, DNA damage and lipid peroxidation in male reproductive organs. Nigella sativa has been identified to ameliorate these adverse effects due to nicotine intake. This study was conducted to evaluate potential protective effects of N. sativa oil on the adverse effects of nicotine on androgen receptors (AR) and ultrastructural features of rat seminal vesicles and prostate glands. Twenty four Sprague Dawley male rats, 7-9 weeks of age and 200-250 g body weight (BW) were randomly divided into; Group 1 Saline (S), forced fed with 0.1 mL/100 g BW of 0.9% normal saline; Group 2 Nicotine (N), intramuscularly injected with 0.5 mg/100 g BW of nicotine; Group 3 N. sativa (NS), forced fed with 6.0 μL/100 g BW of N. sativa and Group 4 Nicotine-N. sativa (NNS), co-administered with 0.5 mg/100 g BW of nicotine and 6.0 μL/100 g BW of N. sativa. The seminal vesicles and prostate glands were extracted after 100 days of treatment. The seminal vesicle and prostate gland were processed for ultrastructural study and androgen receptor detection. The epithelial cells in prostate gland and seminal vesicle of the N group showed weaker brown staining intensity as compared to that of in the NS and NNS groups. This was consistent with the presence of some ultracellular changes observed in the prostate gland and seminal vesicle tissues of the N group. Findings from this study suggested that administration of N. sativa results in ameliorating effects on both the prostate gland and seminal vesicle structures and functions of the nicotine-treated rats. © 2018 Penerbit Universiti Kebangsaan Malaysia. All Rights Reserved..
... It is worth noting that PUFA can influence reproductive processes through several mechanisms. On the one hand, these fatty acids may promote loss of body fat and weight reduction, hence preventing the development of infertility related to obesity; on the other, they control specific aspects linked to male fertility [26,27,68,69]. In fact, these fatty acids are also structural components of cell membranes, whose fluidity is necessary to promote membrane fusion events associated with fertilization. ...
... As observed for avocado extracts as a MUFA source, also walnut extracts, which are good sources of PUFA, reduced sperm shape abnormalities producing improvements in gamete biochemical characteristics [67]. Recently, it has been demonstrated that also oil from Nigella sativa was able to improve sperm quality in infertile men [69]. This oil contains different chemical ingredients including thymoquinone, flavonoids, anthocyanins, alkaloids. ...
Obesity in men of reproductive age is increasing worldwide, impacting negatively on reproductive potential, sperm function and assisted reproduction outcomes. Changes in modern eating behaviors are needed to invert the negative correlation between lifestyle and sperm quality.
It is known from the literature that fatty acids are able to modulate lipid metabolism by complex mechanisms involving a sequence of interdependent and cross-regulated molecular events. Although a great deal of data is currently available on the dietary modulation of lipid metabolism, little is known about the nutritional regulation of sperm energetic metabolism. In this review, major data from the literature about the role of some dietary fats as modulators of sperm function and quality are described. Moreover, biochemical mechanisms responsible for an impairment or improvement in sperm bioenergetics are critically analyzed.
It is noteworthy that both quantitative and qualitative aspects of dietary fats influence sperm function. The administration of a high-fat diet, low in polyunsaturated fatty acids, caused a significant increase in body weight hyperglycemia, hyperinsulinemia and dyslipidemia, along with a significant reduction in sperm quality. In contrast, unsaturated fatty acids are able to positively influence sperm quality and function.
The understanding of the biochemical mechanisms underlying the development of male infertility related to obesity led us to depict a possible framework for the molecular action of fatty acids on sperm quality during dysmetabolic conditions.
... Its seed has a black colour and a bitter flavour. The chemical components of N. sativa include thymoquinone (TQ), anthocyanins, flavonoids, alkaloids, linoleic and oleic acid (Mahdavi et al., 2015). It is grown in the Pakistan, India, Middle Eastern Mediterranean region, South Europe, Syria, Turkey and Saudi Arabia and also indigenous to North Africa, Southern Europe and Southwest Asia. ...
Subfertile males with idiopathic oligoasthenoteratozoospermia (iOAT) can experience a decrease in fertilization and conception rates. Natural antioxidant supplements and herbal treatments recognized to be effective in enhancing fertility. These have the potential to become one of the most efficient medications for treating male infertility. Our study emphasis at how these medication interventions improve several semen parameters while diminishing male factor infertility. A comprehensive literature search using PubMed, Embase, Scopus, Google Scholar and Science Direct were conducted to locate the research papers, focusing the usage of herbal and antioxidant therapies and their favourable effects on various semen parameters on reproductive health. Research based on in vitro and in vivo studies showed that antioxidants, herbal remedies and synthetic therapies have a substantial influence on various sperm parameter. The findings of randomized clinical trials and other preclinical data on exposure of numerous antioxidant supplements, herbal medicines and synthetic therapy demonstrated a considerable benefit to sperm parameters like sperm count, motility, morphology and fertilization rates. It is evident that herbal therapy and proper antioxidant supplementation can improve the rate of conception, so in contrary to synthetic medication, natural and antioxidant therapies are liable to provide better treatment options in managing male fecundity problems.
... With an overall view of the present study, one can justify the benefits achieved by using NS oil for testicular degeneration, which may be attributed to its antioxidant contents, which can enhance steroid hormone synthesis, sperm production, and semen quality [72]. The antioxidant activity and anti-apoptotic properties of thymoquinone (the main constituent of black seed oil) can protect tissue from oxidative injury [65] and inhibit cell death, so it is essential to the integrity of mitochondria by releasing C cytochrome [78], which positively affects spermatogenesis and fertility [79]. In addition, NS oil contains linoleic acid and oleic acid, which have been reported to improve spermatogenesis [73]. ...
The male reproductive function, particularly the testes, and the related hormones are sensitive to various xenobiotics. This work aimed for the first time to assess Nigella sativa floral honey (NS floral honey) and multi-floral honey (M-floral honey) versus Nigella sativa oil (NS oil) against rat testicular degeneration induced with azathioprine (AZA). A total of 40 male Wister rats were assigned into 5 groups: (1) control, (2) 15 mg/kg of AZA, (3) AZA + 1.4 mL/kg of M-floral honey, (4) AZA + 1.4 mL/kg of NS floral honey, and (5) AZA + 500 mg/kg of NA oil. Total testosterone (TT), free testosterone (FT), free androgen index (FAI), gonadotrophins, sex-hormone-binding globulin (SHBG), apoptosis markers, and redox status were assessed to clarify the possible protective mechanisms. Pituitary–testicular axis disruption, apoptosis markers, poor redox status, and sperm quality (count, viability, and motility) were set with AZA. Serum TT, SHBG, and absolute and relative testis weight were significantly restored in the NS oil and NS floral honey groups. Meanwhile, the NS oil group exhibited a significant elevation in FT and FAI. Serum gonadotrophins increased significantly in the NS floral honey (p < 0.01) and M-floral honey and NS oil (p < 0.05) groups. Testicular caspase-3, caspase-9, and nitric oxide showed significant improvement in the NS floral honey and NS oil groups. NS oil supplementation significantly normalized redox status (p < 0.05), whereas NS floral honey improved malondialdehyde and superoxide dismutase activity. Sperm quality exhibited a significant improvement in the NS oil group (p < 0.05). M-floral honey did not show reliable results. Although NS floral honey could protect against testicular damage, it did not upgrade to the level of improvement achieved with NS oil. We claim that further clinical studies are essential for focusing on the quality and quantity of bioactive constituents.
... The patient was prescribed Nigella sativa oil for 2 months. After 2 months it was concluded that daily intake of 5ml Nigella sativa oil improves abnormal semen quality in infertile men without causing any adverse effects [121,122]. ...
It is recommended by the World health organization (WHO) that most of the world's population depends on herbal medicine for their health care. Nigella sativa is commonly known as a Black seed, Black cumin or 'Habbatul Barakah' i.e. an annual herb possessing a wide range of medicinal uses apart from its commercial significance as a spice yielding plant. It has long been used in the folk medicine system of the Arabian Gulf region, Far East Asia, and Europe. Seeds and oils of N. Sativa are the primary medicinal source from ancient times. It is one of the most common herbal plants used worldwide and possesses various chemical constituents such as thymoquinone, thymohydroquinone, dithymoquinone, thymol, nigellicine, carvacrol, nigellicine, nigllimine, nigellidine, and alpha-hederin. There are more than 100 constituents isolated from the plant. These chemical compounds have many pharmacological activities such as antibacterial, antiviral, anti-inflammatory, wound healing effect and also for acne vulgaris, skin cancer, pigmentation and many other cosmeceutical properties. Much scientific research on N. sativa has been conducted over the last five decades to investigate chemical and pharmacological properties. In this review, the phytochemicals, pharmacological properties, Ayurvedic properties and folk uses of Nigella sativa are briefly explained. Keywords: Nigella sativa, Kalonji, Antinephrotic, Immunomodulary, Black cumin.
... Nigella sativa ranunculaceae Nigella sativa L. (Habat Al-Baraka); is a plant that grows widely in Middle Eastern countries. The black seeds are bitter containing different phytochemical structures such as phenolic compounds alkaloids and essential fatty acids [45]. Here in Jordan, it is recommended to use Nigella sativa L. to improve sexual impotence [46]. ...
... Some antioxidants, such as GSH, vitamin C and vitamin E, have been shown to improve semen function and fertilisation capacity in in vitro experimental settings (18,51,60). Similarly, NS has been reported to increase sperm count, mobility and motility, and to improve antioxidant activity in the testis (35). Consistent with previous findings, the results of the present study show that sperm motility reduced by BPA was mostly restored by NS (Fig. 3, 4). ...
The study investigated the protective effect of Nigella sativa (NS) oil against testicular damage induced by bisphenol A (BPA). A total of 36 Wistar Albino rats were divided into 4 groups: control (1ml olive oil), NS (5 ml/kg NS), BPA (100 mg/kg BPA in 1 ml olive oil), BPA+NS (100 mg/kg BPA + 5 ml/kg NS). During the 30-day experiment, all substances were administered by oral gavage. The levels of testosterone and LH in serum, as well as MDA, GSH, SOD, TNF-α, IL-6 and IL-10 levels in testicular tissue were measured by ELISA. Spermatological examinations and histopathological analyses of testicular tissue were also performed. BPA was found to raise LH while reducing the serum testosterone level. BPA caused a decrease in GSH, SOD, TNF-α and IL-6, but an increase in MDA and IL-10. BPA also reduced sperm motility, plasma membrane integrity, mitochondrial activity and acrosome membrane integrity. Oedema, hyperaemia, mononuclear cell infiltration in the interstitial tissue, as well as giant cell formations, degeneration and necrosis in the seminiferous tubules were seen in the BPA group. In addition, reductions in germinative epithelium, tubule diameter and the Johnson testicular score were noted. In rats receiving NS, hormonal profile, oxidative stress markers and cytokine levels were within normal limits. NS improved sperm motility, mitochondrial activity (JC1), plasma membrane integrity (SYBR-14) and partially acrosome membrane integrity (FITC-PNA). NS reduced testis inflammation, significantly ameliorated the testicular score and, to a lesser extent, increased germinative epithelium thickness and tubule diameter. It was concluded that NS has a protective effect against BPA-induced testicular toxicity.
... Extracts of Eurycoma Longifolia Jack showed efficacy or effectiveness in treating male sexual disorders in seven RCTs or NRSIs, but this finding was not reproducible in four other clinical trials (Thu et al., 2017). Beneficial effects of ginger (Zingiber officinale Roscoe) and Black seeds (Nigella sativa L.) were mainly supported by preclinical evidence, although minor clinical evidence was also available (Banihani, 2019;Mahdavi et al., 2015). Two reviews on ginseng (Panax ginseng C.A.Mey.) and Maca (Lepidium meyenii Walp.), respectively, supported their efficacy in enhancing semen quality in infertile men, but the RCT quality was less than satisfactory (Lee et al., 2016(Lee et al., , 2020a. ...
Background
: Male factor infertility often results from testicular disorders leading to inadequate sperm quantity and quality. Both beneficial and detrimental effects of botanical products, especially herbal medicines, on testicular functions and male fertility have been reported in the literature.
Purpose
: This scoping review aims to map the main clinical evidence on the different impacts of botanical entities on the testis and to critically appraise relevant randomized controlled trials (RCTs) published in the recent 5 years, so as to inform the future.
Methods
: Systematic reviews, meta-analyses and RCT reports on botanical impacts on testicular functions and male fertility were retrieved and synthesized from Pubmed, Web of Science, Scopus, Embase, ProQuest, Cochrane Library and Google Scholar up to 10th May 2022. RCTs published since 2018 were critically appraised against good practice guidelines for RCT and for reporting herbal studies.
Results
: We identified 24 systematic reviews and meta-analyses published since 2005, by authors from Iran (25%), China (21%), USA (12.5%) and 9 other countries. All but two were published in English. Only 3 systematic review protocols were identified, all published in English from China in the recent 3 years. We identified 125 RCTs published in six languages, mainly English (55%) and Chinese (42%). They were published since 1994 from 23 countries on all the six inhabitable continents, with China (46%), Australia (8%), USA (8%), India (7%) and Iran (5%) being the leading contributors. 72% and 28% RCTs published in English were on efficacy (botanicals vs placebo) and comparative effectiveness (one botanical vs other treatments), respectively. In contrast, 98% RCT reports in Chinese were on comparative effectiveness, with merely 2% on efficacy. Among all the 125 RCTs, 57% were studies in men with semen abnormality and/or male infertility, 22% investigated herbal effects in healthy men, 14% were in men with male sexual dysfunction and hypogonadism, and 7% were conducted in men with non-sexual disorders. Since 2018, 32 RCTs have been published, in English (69%) or Chinese (31%). Nineteen RCT reports from China, India, Japan and Korea all studied herbal formulae while the 13 RCT reports from Australia, Brazil, Czech and Italy, Iran, Malaysia, Spain, the UK and the USA all exclusively studied extracts of a single species. Putting geo-cultural differences aside, gossypol and extracts of Tripterygium wilfordii Hook. f. were found to be detrimental to the testis and male fertility, while the extracts of Withania somnifera (L.) Dunal and traditional Chinese medicine Qilin Pill, etc., might improve testosterone levels and semen parameters, thus could be therapeutic for male sexual dysfunction and infertility. However, all still require further evaluation in view of recurring weaknesses in quality control of herbal materials, RCT design and reporting. For example, only 9%-23% of the RCTs published since 2018 provided information on voucher samples, chemical profiling, herbal authentication and herbal extraction.
Conclusion
: Research on botanicals and the testis has been reported worldwide, demonstrating clear geo-cultural differences in studied plant species, botanical types, study objectives and quality of research design, implementation and reporting. Due to a few recurring weaknesses in the literature, this study is unable to recommend the use of any specific botanicals, however, current evidence does indicate that botanicals can be double-edged swords to the testis and male fertility. To secure better clinical evidence, future studies must faithfully implement existing and emerging good practice guidelines.
... They have been found to affect steroidogenesis and spermatogenesis positively, in addition to improving reproductive organs and sexual hormones. It was thought that these positive effects of TQ are due to its supporting influence on antioxidant defence (Mahdavi et al., 2015). In the freezing studies of ram semen with different plant extracts, including Moringa Oleifera (Carrera-Chavez et al., 2020), Entada abyssinica (Sobeh et al., 2020), Ginger and echinacea (Merati & Farshad, 2020) extracts, it has been determined that these extracts increase antioxidant activity after thawing and have curative effects on spermatological parameters. ...
The aim of this study was to determine the effects of thymoquinone (TQ), which is the most essential active compound of Nigella sativa, on the spermatological parameters of ram semen during cryopreservation. Ejaculates were collected from five Sonmez rams using an artificial vagina and extended with Tris‐based extender not containing TQ (control, 0 μg/ml TQ) and containing 10, 25, 50 and 100 μg/ml TQ. The extended semen samples were equilibrated in a + 4°C cold cabinet for 2 h. After 2 h, the samples were loaded into 0.25 ml French straws. The straws were frozen by liquid nitrogen vapour and stored in a liquid nitrogen container (−196°C). The frozen straws were thawed in a water bath (37°C for 30 s) and evaluated in terms of motility characteristics, plasma membrane and acrosome integrity, mitochondrial reactive oxygen species levels, lipid peroxidation levels, DNA damage and biochemical alterations (oxidative stress index, malondialdehyde and glutathione). TQ100 had higher total motility (53.59 ± 3.01) and progressive motility (19.84 ± 1.44; not significantly different from TQ25 and TQ50) compared to the control and TQ10 (p ˂ 0.05). According to the results of the analyses on motility characteristics, there were significant differences between the groups in terms of curvilinear velocity (VCL), amplitude of lateral head displacement (ALH) and linearity (LIN; p ˂ 0.05). The highest DNA damage was detected in the control group (p ˂ 0.05). TQ50 had higher plasma membrane and acrosome integrity (59.56 ± 5.92) compared to the control and TQ25 (p < 0.05) but not significantly different from TQ10 and TQ100. The lowest mitochondrial reactive oxygen species levels were detected in TQ50 and TQ100 (p ˂ 0.05). There were no significant differences among the groups in terms of their oxidative stress index, lipid peroxidation, malondialdehyde and glutathione levels (p > 0.05). According to the results, it could be concluded that supplementing 50 or 100 μg/ml TQ to Tris extenders that were used for ram semen cryopreservation showed a positive effect on motility, plasma membrane integrity and acrosome integrity, and it reduced DNA damage and mitochondrial reactive oxygen species levels.
... Botanical Name: Nigella sativa L Local Name: Clongy Medicinal uses: It has been called Miraculous plant and, seeds of blessing due to its antioxidant components have been indicated to improve spermatogenesis and, treat male infertility. 64 It has been used to cure hysteria, common cold, asthma, constipation, urine blockage, anorexia, flatulence, dyspepsia, acute gastroenteritis, dog bites, diabetes, kidney stone, shrinkage milk production, baldness, gas trouble, brain disorders, pneumonia, cough, maleness, appendicitis, labor pain, facial clearness, pimples, fatness, typhoid, piles, swelling, weakness, eczema, diarrhea, malaria, digestive disorders, allergy, wound healer, hearing problems, ear pain, and, swelling, memory increases, paralysis, heart diseases, antiperistalsis, intestinal worms, liver pain, waist pain, sexual weakness, common fever, ascites, menstruation, ulcerative colitis, Croghan's diseases. 65 ...
The present research work is conducted to study the plants, fruits, herbs, and all edibles enlisted in Holy Qur’ān, Aḥādith that have been used by Islamic culture and their health benefits. This paper aims to assemble the latest information about some medicinal edibles and sunnah foods. In the vision of the significance of this study ample data was thoroughly collected from Holy Qur’ān, Aḥādith`s records, Islamic history, and records on the Islamic medicines as well as electronic databases. Current findings are limited to 25 medicinal plants species. Results were systematically arranged of each plant by their English name, along with the botanical names and local names, medicinal uses, and references cited from Holy Holy Qur’ān, Aḥādith, and Islamic books in view of latest scientific research studies. It has been proved that Eras ago, Holy Qur’ān has cited the valuable resources of many foodstuffs, and nowadays scientists have established a portion of them through many studies, which helps to appreciate the vastness of Allah. The paper concludes that the Qur’ān has cited the treasured properties of many foods in preventing diseases, today researchers have recognized a part of them through many experiments, it helps to apprehend the greatness of Allah.
... It have been positive effect on steroidogenesis and spermatogenesis; improve positively reproductive organs and sexual hormones. These ameliorate effect was explained by promoting antioxidant defence (Mahdavi et al., 2015). The current study about ram semen cryopreservation with different plant extract, Morignia Oleifera (Carrera-Chavez et al., 2020); Entada abyssinica (Sobeh et al., 2020); Ginger and echinacea (Meroti and Farshad, 2020) were enhances antioxidant activity and improved spermatological parameters after thawed. ...
This study aimed to determine the protective effects of Thymoquinone (TQ), which is the most essential active compound of Nigella sativa , on the spermatological parameters of frozen‐thawed ram semen. Ejaculates were collected from five Sonmez rams. Ejaculates were extended with control (0 µg/mL TQ), 10, 25, 50 and 100 µg/mL TQ supplemented Tris-based extender. Then extended semen samples were equilibrated in a +4 °C cold cabinet for 2 h. After 2 h the samples loaded into 0.25 mL French straws. The straws were then frozen by liquid nitrogen vapour and stored in a liquid nitrogen container (-196 °C). For analyses, frozen samples were thawed in a water bath (37 o C for 30 s) and evaluated in terms of motility characteristics, plasma membrane acrosome integrity (PMAI), mitochondrial reactive oxygen spices level (MITOSOX+), lipid peroxidation level (BODIPY+), DNA damage and biochemical alterations (oxidative stress index, malondialdehide and glutathione). TQ 100 had higher total and progressive motility compared to control (P˂0.05). According to motility characteristics there were significant differences between the groups on curvilinear velocity (VCL), amplitude of lateral head displacement (ALH) and linearity (LIN; P˂0.05). The highest DNA damage was detected in control groups (P˂0.05). TQ50 had higher PMAI compared to control and TQ25 (P˂0.05). The lowest MITOSOX+ level was detected in TQ50 and TQ100 groups (P˂0.05). There were no significant differences on BODIPY+, oxidative stress index, malondialdehide and glutathione (P>0.05). According to results, it could be concluded that, supplementing 50 and 100µg/mL TQ to Tris extender that was used for ram semen cryopreservation, showed a positive effect on motility, PMAI and also decreased DNA damage and MITOSOX+.
... Therefore, the plant of herbal medicine that possesses antioxidant properties is off interest to scavenging free radicals. NS has been shown several beneficial properties including antioxidant that modulate both spermatogenesis and steroidogenesis 29 . In the present study, EB showed clear negative effects on antioxidant activity, sperm markers, fertility hormones, and normal testicular structures. ...
Background: Food and water that contained metals and chemicals, contaminated air, extensively used of medicinal substances and cosmetic materials can induce infertility in several ways, and therefore increased numbers of couples not conceive globally. Aim: Investigation the toxic impact of ethidium bromide (EB) on testicular function and the therapeutic role of Nigella sativa (NS) and silver nanoparticles (AgNPs). Materials and methods: A total of 50 male Wistar rats were assigned randomly into five equal groups; control (C) negative and four experimental which respectively received EB only, EB and NS, EB and AgNPs, and EB, NS, and AgNPs for 30 days. Post-experimental period, blood and testicular tissues were collected to determine the antioxidant activity, fertility hormones level, sperm quality and quantity, and histological structure of the testis. Results: In comparison to control, EB group exhibited significant reduction (P<0.05) in antioxidants (catalase (CAT), superoxide dismutase (SOD), and glutathione perioxidase (GPX)), sperm parameters (quantity, morphology, viability and motility), fertility hormones (FSL, LH, and testosterone), and clear histological defects (atrophy in seminiferous tubules, spermatozoa and spermatogonia apoptosis, vacuolization, congestion and thrombus in seminiferous tubules septum, and leydig cells degeneration). However, all these negative effects were significantly reversed in response to supplement of NS, AgNPs, and mixed of them. These including modulate antioxidants activity, sperm’s quantity and quality, fertility hormones, and testicular tissue structure. Conclusion: NS and AgNPs possess interesting properties that have ability to reverse negative impacts of EB, and therefore could be alternative and safe therapeutic agents for infertility.
... All BS and THY treatments reduced the abnormal and nonviable sperm percentages due to the antioxidant properties of BS and THY, which offer more protection against oxidative stress (Mahdavi et al., 2015). The THY100 treatments had high sperm concentrations, total sperm outputs and total sperm fractions. ...
The quality of incubated or chilled rabbit semen is quickly lowered with time due to high‐rate production of reactive oxygen species (ROS). Black seed (BS) and thyme (THY) are rich sources of natural antioxidants, which may be able to control ROS production and improve semen quality for use in artificial insemination. In this study, V‐line rabbit bucks, seven months old, were fed diets supplemented with 50 or 100 g of BS or THY for 60 days. Semen was collected twice weekly. The quality of fresh sperm was measured, and diluted sperm was incubated for up to 12 h. Chilled, diluted sperm, stored at 4℃ for up to 3 days, was evaluated. The results indicated that BS and THY increased rabbit libido, decreased abnormal sperm and non‐viable sperm percentages and significantly lowered total bacteria counts of diluted sperm. In conclusion, bucks fed a diet supplemented with BS or THY had enhanced semen quality and storage life, because of the antioxidant properties of BS and THY. The best incubation results were obtained with the THY100 treatment, whereas the best‐chilled results were obtained with the BS100 group.
... In the past 2 decades, various pharmacological or medicinal aspects of black seed (BS) including its antibacterial, antioxidant, anticancer, antiinflammatory, immuno-modulatory, analgesic, diuretic, antihypertensive, neuroprotective, antidiabetic and hepatoprotective properties, have been reported [13][14][15] . Also, it has been used in clinical research for neurological disorders, hyperlipidemia, obesity, lung disease, hypertension, thyroid dysfunction, hepatitis, rheumatoid arthritis and male infertility [16][17][18][19][20] . Furthermore, it has shown to decrease the replication of SARS-CoV in vitro in cell cultures 21. ...
In early 2020, the world health organization (WHO) declared Covid-19 as a global pandemic. Little is known about the potential protective factors against this viral disease. This study aimed to evaluate the preventive role of black seed (BS) in reducing infection rates with Covid-19 in people involved in the study. It is a descriptive, non-randomized and open-label study in which (376) participants were divided into two equal groups. The control group (CON) included participants who didn't take the black seeds, while the (BS) group included participants who took black seeds once daily at a 40 mg/kg orally. Knowing that the prophylactic efficacy of the black seed was seen through a smaller percentage of infected people at risk. A significantly lower infection rate was observed among black seed group 68 (36.2%) in comparison with the control group 180 (95.7%). Also, the (BS) group showed reduced incidence of infection at intermediate 12 (17.6 %) and low 0 (0.0 %) levels of exposure to risk factors. Therefore, we can conclude that black seed has the ability to reduce infection rates of covid-19 in people at risk.
... In the past 2 decades, various pharmacological or medicinal aspects of N. sativa including its antibacterial [5], anticancer [6,7], anti-inflammatory [8], antioxidant [9,10], immunomodulatory [11], analgesic [12][13][14], diuretic [15,16], antihypertensive [17], antidiabetic [18][19][20], neuroprotective [16], gastroprotective [21,22], and hepatoprotective properties [23], have been reported. N. sativa has been used in clinical research for neurological disorders [24][25][26], hypertension [27,28], hyperlipidemia [29,30], obesity [31,32], rheumatoid arthritis [33][34][35], lung disease [36], thyroid dysfunction [37], hepatitis [38], and male infertility [39]. ...
Nigella sativa (commonly known as black seed or black cumin), from the family Ranunculaceae, is a plant that grows in countries bordering the Mediterranean Sea. This narrative review discusses the toxicological profile reported by short-to long-term studies that examined different extracts and oils of N. sativa seeds. Scientific databases including Web of Science, PubMed, Scopus, and Google Scholar were searched using appropriate keywords. LD 50 for administered N. sativa seed fixed oil varied from 28.8 mL/kg to 3,371 mg/kg in mice, while 21 g/kg of aqueous, methanol, and chloroform extracts of N. sativa did not lead to any mortality. Subacute toxicity evaluations indicated that aqueous, methanol, and chloroform extracts of N. sativa at doses as high as 6 g/kg do not produce toxicity. Investigation of chronic toxicity found that 2 mL/kg of N. sativa fixed oil is slightly toxic. Cytotoxicity studies indicated that N. sativa chloroform and petroleum ether extracts are more cytotoxic than its other extracts. Although studies that assessed N. sativa toxicity generally introduced it as a safe medicinal herb, to draw a more definitive conclusion on its safety, more detailed studies must be conducted.
... There is a need to lead pre clinical and clinical investigations to help customary cases and to work out cell and subatomic system associated with nicotine initiated barrenness. Nicotine, a pharmacologically dynamic alkaloid, is the most important part in tobacco (Mahdavi et al., 2015). A few investigations have referenced that the initiation of Tobacco utilization takes puts in youngsters. ...
Numerous examinations have announced that the Nicotine present in tobacco smoke adverse affects sperm parameters, fundamental plasma, and different other richness factors. These harms are because of the natural finding that smoking expands the degree of free radicals, consequently bringing about oxidative pressure. Accordingly, an increment of free radicals, DNA harm and lipid peroxidation in human sperm may happen to bring about impedance of sperm quality. nicotine organization in trial creatures was initiate to influence spermatogenesis, epididymal sperm count, motility and treating capability of sperms. Huge quantities of restorative herbs have been recorded in siddha framework that improve spermatogenesis and increment the nature of sperm. The present review gives an outline on the relationship between male richness incited by nicotine and defensive impacts of different natural plants on it. The paper additionally features the dynamic, common standards, and rough concentrates of plants, which have been valuable in improving nicotine instigated testicular harms and supportive in spermatogenesis and multiplication. Despite the fact that the discoveries of this survey propose numerous herbs are extremely viable against nicotine actuated male barrenness still increasingly clinical preliminaries are prescribed to show their viability on nicotine instigated male infertility
... Thousands of research articles that have been published in international medical journals are available on the internet regarding studies on the medicinal properties the seeds of black cumin (BC, Nigella sativa L.), their oil and their bioactive compounds (Randhawa, 2008). Over the last two decades, several reviews have provided comprehensive information on the nutritional, health, pharmaceutical, therapeutic and biomedical applications and prospects of BC (Randhawa and Al-Ghamdi, 2002;Ali and Blunden, 2003;Gilani et al., 2004;Salem, 2005;El-Tahir et al., 2006;Akhondian et al., 2007;Ramadan, 2007;Ismail and Yaheya, 2009;Sharma et al., 2009;Paarakh, 2010;Randhawa and Alghamdi, 2011;Naz, 2011;Shrivastava et al., 2011;Mirzaei, 2012;Datta et al., 2012;Ahmad et al., 2013;Alenzi et al., 2013;Azeem et al., 2014;Forouzanfar et al., 2014;Mollazadeh and Hosseinzadeh, 2014;Razavi and Hosseinzadeh, 2014;Tembhurne et al., 2014;Aljabre et al., 2015;Bamosa, 2015;Gali-Muhtasib et al., 2006;Gholamnezhad et al., 2015;Heshmati and Namazi, 2015;Longato et al., 2015;Mahdavi et al., 2015;Majdalawieh and Fayyad, 2015;Amin and Hosseinzadeh, 2016;Beheshti et al., 2016;Gholamnezhad et al., 2016;Hayatdavoudi et al., 2016;Hussain and Hussain, 2016;Khan et al., 2016;Kooti et al., 2016;Mohtashami and Entezari, 2016;Sahebkar et al., 2016aSahebkar et al., , 2016bShokri, 2016;Ijaz et al., 2017;Tavakkoli et al., 2017;Mazaheri et al., 2019). ...
This review outlines the current state of knowledge on the nutritive value of black cumin (Nigella sativa L.). The popularity of this plant, which is an annual herbaceous plant belonging to the Ranunculaceae family and is native to Iran, Pakistan and Turkey, is due to its beneficial actions. Black cumin has many therapeutic effects and is considered one of the most important medicinal plants in the world because of its antioxidant, anticoccidial, anthelminthic and antimicrobial activities. The nutritive value of black cumin is result of its carbohydrate, fatty acid, protein contents as well as its several bioactive compounds. The seeds or their byproducts can be used in feeds for farm animals, with positive effects on the compositional characteristics of eggs, milk and meat.
... Among previous published systematic review and meta-analysis investigating the effectiveness of N.S [63][64][65][66][67][68], no reports were targeted on liver or kidney function tests. Most notably, since none of the human trials assessed the exact mechanisms of the effects of N.S or black seed family, most of the published mechanisms were investigated either in animal or in in-vivo and in-vitro studies [69][70][71][72]. ...
The aim of this systematic review and dose–response meta-analysis was to determine the effect of Nigella Sativa (N.S) supplementation on liver and kidney parameters. We searched PubMed, Scopus, ISI Web of Science, Cochrane central register for controlled trials and Google Scholar from database inception to April 2019 for relevant controlled trials. Mean differences and standard deviations for each outcome were pooled using a random-effects model and a dose–response analysis was performed using a fractional polynomial model. Quality of evidence was evaluated using Cochrane Collaboration Risk of Bias tool and the Grading of Recommendations Assessment, Development and Evaluation (GRADE) methodology. Nineteen trials (n = 1295 participants) were included in the meta-analysis. We observed that N.S supplements had significant reducing effects on alkaline-phosphatase (ALP) [9 trials, n = 710 participants, weighted mean difference (WMD)= -10.825; 95%CI: -19.658, -1.992 U/L; P = 0.016; I² = 75.7 %; P-heterogeneity = 0.000) and blood urea nitrogen (BUN) (12 trials, n = 821 participants, WMD= -1.016; 95% CI: -1.760, -0.273 U/L; P = 0.007; I² = 87.7%; P-heterogeneity = 0.000) concentrations. Subgroup analysis showed that, an intervention of more than 12 weeks was found to have a reducing effect on aspartate- aminotransferase (AST) measures (2 trials, n = 201 participants, WMD= -11.317; 95%CI: -15.007, -7.626; P = 0.000; I² = 0.0%; P-heterogeneity = 0.977). Creatinine levels increased significantly in studies that considered adjusted analysis based on covariates (3 trials, n = 152 participants, WMD = 0.070; 95% CI: 0.027, 0.112 U/L; P = 0.001; I² = 0.0%; P-heterogeneity = 0.788). A daily dose of 1100-1500 mg of N.S supplements was observed to have a substantial reducing effect on ALP levels (5 trials, n = 340 participants, WMD= -11.323; 95% CI: -21.418, -1.229 U/L; P = 0.028; I² = 0.00%; P-heterogeneity = 0.686), while a dosage of more than 2000 mg per day led to a significant increase in BUN concentrations (2 trials, n = 101 participants, WMD= -1.016; 95% CI: -1.760, -0.273 U/L; P = 0.007; I² = 87.7%; P-heterogeneity = 0.000). Our data suggested that N.S supplementation had significant impacts on liver and kidney parameters leading to a decrease in ALP and BUN levels. Longer duration of intervention and normal daily dosages of N.S supplements led to significant reductions in ALP and AST concentrations, respectively, while higher daily dosages increased BUN levels. Hence, in spite of favorable impacts of N.S supplements on liver and kidney parameters, due to the herbal nature of N.S, more studies with high-quality, large-scale, long-term intervention and precise baseline characteristics are needed to assess the exact effective dose, duration and efficacy of N.S supplementation on kidney and liver parameters.
... Apparently, there are yet no clinical trials conducted in infertile men treated with thymoquinone except the only one study that evaluated the effects of N. sativa oil in childless men (Kolahdooz et al., 2014). Consequently, to date there is insufficient evidence to make recommendations for the use of thymoquinone as an adjunct therapy in infertile men (Mandavi, Heshmati, & Namazi, 2015). However, based on the promising effects of black seed on the fertility health of different animal models, more clinical trials are required to further demonstrate the efficacy of N. sativa on male infertility. ...
This review attempts to collate existing data and provide the perspectives for future studies on the effects of plants on the male gonads. For many of these medicinal plants such as Lepidium meyenii, Rupus coreanus, Tribulus terrestres, Panax ginseng, Petasites japonicas, Apium graveolens, Eurycoma longifólia, Pedalium murex, Corchorus depressus, Mucuna pruriens, Astragalus membranaceus, Nigella sativa, Crataegus monogyna, Fagara tessmannii, Phaleria macrocarpa, Anacyclus pyrethrum, Cynomorium songaricum and Morinda officinalis, the mechanism of actions of their active principles and crude extracts has been shown in both laboratory animals, in vitro, and human studies, and includes their antioxidant, anti‐inflammatory, spermatogenesis‐inducing, aphrodisiac, smooth muscle relaxing and androgenic properties. Several active chemical leads including glucosinolates, anthocyanins, protodioscin, ginsenosides, sesquiterpenes, phyto‐oestrogens, quassinoids, diosgenin, thymoquinone, proanthocyanidins and bajijiasu isolated from these plants are known to have target effects on the testis, but efforts have been limited in their application at the clinical level. There still appear to be many more extracts of medicinal plants that have not been characterised to determine the phytochemicals unique to them that have target effects on the gonads. Further, collaborative efforts at isolating pro‐drug candidates from medicinal plants for studies at the molecular, cellular and clinical level towards elucidating their mechanisms of action on the testes are therefore warranted in the light of the current male fertility crisis.
... Tribulus (Tribulus terrestris L., Zygophyllaceae) is the main phytotherapics which is promoted to increase testosterone and improve sperm characteristics in humans [57][58][59][60], long Jack (Eurycoma longifolia Jack, Simaroubaceae) [61,62], fineleaf fumitory (Fumaria parviflora Lam. Ranunculales) [11]., Onion (Allium cepa L. Amaryllidaceae) [63] and black seeds (Nigella sativa L., Ranunculaceae) [64,65]. Although, all these evidences based on the therapeutic effects of herbal medicine on male infertility are on animal models and spermatogenic effect on human and its little clinical attestation is yet to be investigated. ...
Background:
Infertility and gonadal dysfunction are well known side-effects by cancer treatment in males. In particularly, chemotherapy and radiotherapy induced testicular damage, resulting in prolonged azoospermia. However, information regarding therapeutics to treat spermatogenesis disturbance after cancer treatment is scarce. Recently, we demonstrated that Goshajinkigan, a traditional Japanese medicine, can completely rescue severe busulfan-induced aspermatogenesis in mice. In this study, we aimed to detect the effects of Goshajinkigan on aspermatogenesis after irradiation.
Methods:
This is animal research about the effects of traditional Japanese medicine on infertility after cancer treatment. C57BL/6 J male mice received total body irradiation (TBI: a single dose of 6Gy) at 4 weeks of age and after 60 days were reared a Goshajinkigan (TJ107)-containing or TJ107-free control diet from day 60 to day 120. Then, two untreated females were mated with a single male from each experimental group. On day 60, 120 and 150, respectively, the sets of testes and epididymis of the mice in each group after deep anesthetization were removed for histological and cytological examinations.
Results:
Histological and histopathological data showed that 6Gy TBI treatment decreased the fertility rate (4/10) in the control diet group; in contrast, in the TJ107-diet group, the fertility rate was 10/10 (p < 0.05 vs. 6Gy group). Supplementation with TJ107 was found to rescue the disrupted inter-Sertoli tight junctions via the normalization of claudin11, occludin, and ZO-1 expression and reduce serum anti-germ cell autoantibodies.
Conclusions:
These findings show the therapeutic effect on TBI-induced aspermatogenesis and the recovering disrupted gonadal functions by supplementation with TJ107.
... They concluded that black seed could have positive effects on sperm and semen parameters, leydig cells, reproductive organs, and sex hormones. 22 In the mentioned studies, the positive effects of black seeds on male fertility were reported. In the present study, these positive effects were observed in an aging mouse model. ...
Background: The aging process is accompanied by low secretion of sex hormones and testicular apoptosis. The antioxidant properties of thymoquinone (TQ) may prevent the effects of aging. Therefore, in the present study, the effects of different doses of TQ were investigated on sperm parameters, testosterone level, apoptosis, and oxidative stress in a mouse model of D-galactose-induced aging. Methods: In this experimental study, 30 adult male mice were randomly divided into 5 groups. The control group did not receive any injections, while the D-galactose group received an intraperitoneal injection of 300 mg/kg of D-galactose for 42 days. The TQ1-TQ3 groups received intraperitoneal injections of 5, 2.5, and 1.25 mg/kg of TQ plus Dgalactose, respectively for 14 days (from the 1st to the 14th day of the experiment). The morphometric analysis, testicular apoptosis examination, and sperm analysis were performed, and testosterone level, total antioxidant capacity, and malondialdehyde level were evaluated on day 42 of the experiment. Data were analyzed using SPSS. Results: Administration of TQ in the TQ1 group caused a significant difference in sperm parameters, compared to the D-galactose group (P<0.05). The lowest amount of positive tunnel cells was related to 5 mg/kg of TQ and the highest to 2.5 mg/kg of TQ. There was no significant difference in the parameters of seminal vesicles, epididymis, prostate, and testis between the groups (P>0.05). The malondialdehyde level were decreased in the TQ1-TQ3 groups, compared to the D-galactose group (P<0.001). On the other hand, the total antioxidant capacity was increased significantly in the TQ1 group, compared to the Dgalactose group (P<0.001). Conclusion: Administration of 5 mg of TQ for 14 days improved sperm quality and biochemical parameters, while reducing apoptotic cells of the testes in a mouse model of aging.
... Nigella sativa (NS), also known as black seed, is from Ranunculaceae family that is cultivated in Asia (17)(18)(19)(20). The main components of NS are thymoquinone (TQ), unsaturated fatty acids, flavonoids, nigellone, p-cymene and carvone (19,(21)(22)(23)(24)(25)(26)(27). ...
Context: Nigella sativa (NS) has been used as an herbal remedy for the treatment and prevention of a variety of diseases. In this review, we aimed to summarize the current evidence on the effects of NS consumption on non-alcoholic fatty liver disease (NAFLD) characteristics. Evidence Acquisition: We reviewed the existing literature published by the end of 2017 using the following key words: “Nigella sativa”, “black seeds”, “black cumin”, “thymoquinone”, “NAFLD”, “NASH”, and “diabetes”. Papers used in this study were collected by searching the PubMed, Google Scholar, Science Direct and Scopus databases. Our search was limited to English-language articles. All the articles published between 2000 and 2017 meeting the inclusion criteria were included in the study. Results: The results of current studies indicate that NS has many biological effects such as anti-inflammatory, anti-hyperlipidemic, anti-microbial, anti-cancer, anti-oxidative, anti-diabetic, anti-hypertensive and wound healing activities. In summary, it can be used as a valuable plant for designing therapeutic strategies in NAFLD. Conclusions: Results from available studies indicate that NS can ameliorate the main metabolic disturbances related to NAFLD including hyperglycemia, hyperlipidemia, and overweight. These effects are mainly attributed to the anti-oxidative and anti-inflammatory properties of thymoquinone. Clinical trials on human subjects are highly essential to confirm the results found in in vivo and in vitro studies.
... It is worth noting that PUFA can influence reproductive processes through a variety of mechanisms. On the one hand, these fatty acids may promote loss of body fat and weight reduction, hence preventing the development of infertility related to obesity; on the other, they control specific aspects linked to male fertility (Lenzi et al., 2000;Wathes et al., 2007;Esmaeili et al., 2015;Mahdavi et al., 2015). Moreover, PUFA provide the precursors for eicosanoids synthesis that can modulate the expression patterns of many key enzymes (such as cyclooxygenase and lipoxygenase) involved in both prostaglandin and steroid metabolism; these fatty acids are also structural components of cell membranes, whose fluidity is necessary to promote membrane fusion events associated with fertilization. ...
Recently, obesity has been linked to male infertility. In animal models the administration of a high-fat diet caused a reduction in sperm quality, by impairing gamete energy metabolism. The aim of this study was to investigate a possible effect of dietary fatty acids supplementation in the modulation of sperm energy metabolism and, in turn, in the improvement of sperm quality in rats fed a high-fat diet. Sexually mature male Sprague–Dawley rats were divided into four groups and fed for 4 weeks a standard diet (con- trol group), a high-fat diet (enriched in 35% of fat and 15% sucrose), a high-fat diet supplemented with 2.5% olive oil (a source of monounsaturated fatty acids) or a high-fat diet supplemented with 2.5% krill oil (a source of n-3 polyunsaturated fatty acids). Liver and adipose tissue weight, plasma glucose, insulin and lipid concentrations were determined. Activities of enzymes involved in sperm energetic metabolism were evaluated by spectrophotometric assays. Sperm mitochondrial respiratory efficiency was also assayed. The obtained results suggest that olive oil partially counteracts the negative effects of a high-fat diet on sperm quality, by increasing gamete motility, by reducing oxidative stress and slightly improving mitochondrial respiration efficiency. On the other hand, krill oil determines an increase in sperm concentration and motility, an increase in the activities of lactate dehydrogenase, Krebs cycle enzymes and respiratory chain complexes; a parallel increase in the cellular levels of ATP and a reduction in oxidative damage were also observed. These results suggest that dietary fatty acids are able to positively influence sperm quality and function.
... Moreover, the ethanol extract of NS at 250-100 mg/kg (p.o.) in female Wistar Albino rats showed a significant nephroprotective activity against paracetamol-induced nephrotoxicity 33 . In another study, the NS exhibited a significant nephroprotective effect in Cdinduced nephrotoxicity in rats 34 . ...
Nigella sativa L. (NS) is evident to have a number of important biological activities, such as antioxidant, anti-inflammatory, antibacterial, antifungal, anti-viral, anti-parasitic and anti-protozoal, cytotoxic, anticancer, neuro-, gastro-, cardio-, hetapto- and nephro-protective activities. In addition, the NS implies beneficiary effects on reproductive, pulmonary and immune systems as well as in diabetes mellitus (DM), fertility (male and female), breast cancer, dermatological complications, dehydration, dyspepsia, and osmotic balance and so on. Among the other isolated constituents, thymoquinone (TQ) is a vastly studied phytochemical in NS. A number of reports suggest that the NS may be one of the potential herbs in health promotion. This paper will discuss the current scenario of NS activity in a mechanistic way.
Nigella sativa, commonly known as “black cumin” or “black seed”, is an annual flowering plant in the family Ranunculaceae. It is widely distributed in North Africa, the Middle East, Europe, and Asia. This chapter summarizes a research update on the pharmacological activities of N. sativa. Based on research thymoquinone (TQ) is a major bioactive compound in N. sativa that significantly contributes to its therapeutic effects. N. sativa has also been reported to exhibit antidiabetic, antioxidant, anti-inflammatory, antimicrobial, analgesic, and immunomodulatory properties. It is emphasized that further research is needed to fully elucidate its potential and mechanisms of action.
Oils derived from plant sources, mainly fixed oils from seeds and essential oil from other parts of the plant, are gaining interest as they are the rich source of beneficial compounds that possess potential applications in different industries due to their preventive and therapeutic actions. The essential oils are used in food, medicine, cosmetics, and agriculture industries as they possess antimicrobial, anticarcinogenic, anti-inflammatory and immunomodulatory properties. Plant based oils contain polyphenols, phytochemicals, and bioactive compounds which show high antioxidant activity. The extractions of these oils are a crucial step in terms of the yield and quality attributes of plant oils. This review paper outlines the different modern extraction techniques used for the extraction of different seed oils, including microwave-assisted extraction (MAE), pressurized liquid extraction (PLE), cold-pressed extraction (CPE), ultrasound-assisted extraction (UAE), supercritical-fluid extraction (SFE), enzyme-assisted extraction (EAE), and pulsed electric field-assisted extraction (PEF). For the identification and quantification of essential and bioactive compounds present in seed oils, different modern techniques—such as high-performance liquid chromatography (HPLC), gas chromatography–mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), gas chromatography–infrared spectroscopy (GC-IR), atomic fluorescence spectroscopy (AFS), and electron microscopy (EM)—are highlighted in this review along with the beneficial effects of these essential components in different in vivo and in vitro studies and in different applications. The primary goal of this research article is to pique the attention of researchers towards the different sources, potential uses and applications of oils in different industries.
Nature offers us spices, which are a significant part of healthy and nutritious foods. The presence of abundant bioactive compounds in these spices makes them interesting from a scientific and health perspective. Extracts obtained from spice materials possess many health benefits and are rich sources of antioxidants, which suppress reactive oxygen species.
Spice Bioactive Compounds: Properties, Applications, and Health Benefits collects such information together in one book, presenting all necessary features related to spices and their properties. Exploring the most recent research related to the extraction, isolation, encapsulation, identification, and characterization of bioactive compounds present in spices, this book also covers the health element of spices and its utilization as a treatment for various disorders.
Key Features:
Discusses about 14 different spices and their salient features
Presents the novel technologies used in the extraction, isolation, and identification of bioactive compounds from spices
Explores the utilization of spices for culinary use in food
Industries such as the food and pharmaceutical industries have great interest in the use of bioactive compounds for the production of drugs and functional foods. Written by experts in their field, this book will be useful to anyone in either industry, as well as those who have an interest in the use of such bioactive compounds for the production of drugs and functional foods.
Oleoresin is a mixture of volatile and nonvolatile components available in whole extract of natural herb or spice. It principally comprises essential oils and resin. Lemongrass oleoresins come from the Cymbopogon species, which grow in the tropical and subtropical regions of the world. Oleoresin of lemongrass is a dark green-colored viscous liquid having a characteristic lemon aroma and flavor and is mostly used as a flavoring ingredient. The lemon prefix in the lemongrass specifies the characteristic lemon-like odor, which is due to the availability of citral content (mixture of two isomeric aldehydes, geranial and neral). It has been utilized in synthesizing flavors, perfumes, cosmetics, detergents, and in the food and pharmaceutical industries. Different methods are used to extract the lemongrass essential oil, but steam distillation is the most suitable method as it doesn’t alter the quality of the obtained oil. The chemical composition of lemongrass oil varies depending on its extraction methods, genetic differences, harvest period, photoperiod, plant age, farming practices, and geographical origin. Lemongrass essential oil has shown several biological activities, including antimicrobial, antifungal, antiprotozoan, antioxidant, antidiarrheal, antimutagenic, antiinflammatory, antimalarial, antinociceptive, antihepatotoxic activities, etc. Lemongrass oil is a potent food preservative because of its extraordinary antifungal and antibacterial activities.
Nigella sativa plant from Ranunculaceae family has been commonly used as traditional remedies by the ancient world such as Greeks, Romans, and Egyptians. The plant is also known black seed or black cumin. The plant is highly valued by Muslims all over the world as it has been mentioned by the Islamic Prophet Muhammad that the black seed has the capability of curing all diseases except death. The purpose of this chapter is to provide updated and categorized information on the traditional uses, chemical composition, biological activities, bioavailability, safety, toxicity, and clinical trials of N. sativa in order to explore their therapeutic potential and evaluate future research opportunities. Every part of this plant contains a valuable medicinal feature. It contains different types of active phytoconstituents like carbohydrates, volatiles, alkaloids, saponins, flavonoids, phenolics, glycoside, coumarins, fixed oils, proteins, vitamins, and minerals are present. The use of its seeds, whole plant, and oil is common for treatment of many diseases like hepatoprotective, antidiabetic, antiasthmatic, cardioprotective, analgesic, neuroprotective, antiinflammatory, antioxidant, antimicrobial, and anticancer effects. N. sativa has potential for the treatment of a wide range of diseases and has been well studied for its phytochemical properties. However, further scientific studies are needed to explore mechanisms of actions, adverse effects of the extracts, the effective therapeutic dose, and the therapeutic effect of major secondary metabolites.
Most of the preclinical studies based on animal models found that Nigella sativa extract in any form such N. sativa or the powdered form suggest its positive impact on the endocrine system. In particular, N. sativa appears helpful with regard to restoring the healthy histological architecture of endocrine tissue or its active constituents. In a general sense, it has manifested potential to directly remodel/modify their function, mitigating the hormonal disturbances seen in most common endocrine disorders such as thyroid dysfunction, metabolic, adrenal, and gonadal disorders. The therapeutic action of N. sativa is largely contributed by thymoquinone, a monoterpene compound. This monoterpene acts as a molecular scavenger of reactive oxygen species. It protects the endocrine tissue from the free radical species’ damaging effect and contributes in rebalancing hormonal disturbances. More extensive and rigorous preclinical research in combination with clinical studies can unravel the therapeutic properties and features of N. sativa in the context of specific endocrine disorders. At the same time working on technological novelties capable of improving its bioavailability and penetration potential is quite promising, while refining the regulatory framework of this research and its potential clinical implications is necessary.
This study was aimed to evaluate therapeutic effects of thymoquinone on male reproductive damages induced by paclitaxel. Forty‐eight male rats were divided; control, paclitaxel (4 mg/kg), paclitaxel + thymoquinone (1.25, 2.5 and 5 mg/kg) and thymoquinone (1.25, 2.5 and 5 mg/kg). Paclitaxel and thymoquinone were administrated intraperitoneally for 4 and 14 days respectively. Then, the testes were removed for H&E staining, sperm parameters and apoptotic genes expression assessments. Serum levels of nitric oxide, total antioxidant capacity and testosterone were evaluated, and sperm DNA fragmentation was assessed. Paclitaxel significantly (p < .05) increased nitric oxide, decreased total antioxidant capacity and reduced testosterone levels than control group. Sperm motility, viability and count were significantly (p < .05) reduced in paclitaxel group than control. Co‐administration of thymoquinone + paclitaxel caused decreased levels of nitric oxide and increased total antioxidant capacity, testosterone levels and reproductive parameters than paclitaxel group significantly (p < .05). Paclitaxel significantly (p < .05) increased caspase‐3 and p‐53 and decreased Bcl‐2 genes expression than control. Sperm DNA fragmentation index was also increased significantly (p < .05) in paclitaxel group than control, and this value was decreased in whole doses of paclitaxel + thymoquinone groups than paclitaxel. Thymoquinone can alleviate the side effects of paclitaxel on the male reproductive system.
This study was carried out in order to determine the effect of different
levels of rosemary (Rosmarinus officinalis L.) essential oil on performance parameters, some serum characteristics and reproductive hormones of male Japanese quails (Coturnix coturnix Japonica). In the study, 60 male Japanese quails at the age of 49 days were used as animal material. The study was conducted with 5 replications in 4 treatment groups, in 20subgroups with 3 animals in each cage, for 3 weeks. In the study, 4 different mixed feeds were prepared, one of which was control (0 mg/kg) and 3 of them at different levels (100, 200 and 400 mg/kg) of rosemary essential oil. At the end of the study, it was determined that the effect of rosemary essential oil addition to male quail rations on body weight gain and glucose, among serum parameters, were statistically significant (P<0,05). On the other hand, it was seen that its effect on the other parameters was insignificant (P> 0,05).
The present study was aimed to identify the localization of thymoquinone in vegetative parts, viz. leaf, stem, root, flower forming bud, flower, and seed forming bud of Nigella sativa plant. Isocratic RP-HPLC method was used with mobile phase methanol/water 70:30, C-18 column, detection wavelength 254 nm (confirmed by spectrum scanning), flow rate of 1.0 ml/min, retention time 8.8 min. A linear standard calibration curve of thymoquinone was prepared with R2 as 0.9569 and % RSD as 20.7518. The results showed maximum yield in seed forming bud (6.433 ± 0.450%) using benzene as solvent, whereas the methanol extract has minimum yield in stem (2.200 ± 0.300). Maximum thymoquinone content was found in seed forming bud with (0.0648 ± 0.0038%) and (0.7830 ± 0.0360%) in methanolic and benzene extracts (washed with ethanol; EtOH), respectively. It was further observed that benzene extract (EtOH) was about 12-fold more than methanolic extract (EtOH). When total antioxidant activity was explored using DPPH free radical assay, it was found maximum in flowering bud (77.076 ± 5.889%), while minimum was observed in roots (47.933 ± 4.003%). When correlation was established between methanolic (EtOH) and benzene extracts (EtOH) with antioxidant activity, a strong positive correlation of 0.9535 and 0.7406, respectively, was observed. Thus, it is evident that the level of thymoquinone content in different plant parts is analogous to its antioxidant activity. Therefore, it can be concluded that benzene (EtOH) is the better solvent for extraction of thymoquinone from Nigella sativa; further, seed forming bud is a better source of thymoquinone which can be useful in nutraceutical and pharmaceutical industry for better herbal drug formulations.
An in depth review on Nigella sativa and its derived constituents has been necessitated which has been sketched in this paper from the research reports obtained from PubMed and ScienceDirect databases. Findings of this meticulous review suggest that N. sativa possesses various important phytoconstituents and derived compounds with diverse biological effects including antioxidant, anti-inflammatory, anti-bacterial, anti-fungal, anti-parasitic, anti-protozoal, antiviral, cytotoxic, anticancer, and neuro-, gastro-, cardio-, hepato- and nephro-protective activities. In addition, N. sativa implies beneficiary effects on reproductive, pulmonary and immune systems along with diabetes mellitus (type 2 diabetes), fertility, breast cancer, dermatological complications, dehydration, dyspepsia, osmotic balance and others. Amongst several isolated chemical moieties of N. sativa, thymoquinone may be one of the best targets for treatment of microbial infections, inflammations, cancer, metabolic syndromes, and many other diseases. The N. sativa is evident to promote health in some non-clinical and clinical studies and it may serve to be one of the best sources for modern phyto medicine.
Objectives
Nigella sativa (black seed or black cumin), which belongs to the Ranunculacea family, is an annual herb with many pharmacological properties. Among its many active constituents, thymoquinone (TQ) is the most abundant constituent of the volatile oil of Nigella sativa (N. sativa) seeds, and it is the constituent to which most properties of this herb are attributed.
Methods
PubMed-Medline, Scopus, and Web of Science databases were searched to identify randomized control trials (RCTs) investigating the therapeutic effects of N. sativa and/or TQ. In this review, we investigated the clinical uses of N. sativa and TQ in the prevention and the treatment of different diseases and morbidity conditions in humans.
Results
Black seed and TQ are shown to possess multiple useful effects for the treatment of patients with several diseases, such as inflammatory and auto-immune disorders, as well as metabolic syndrome. Also, other advantages, including antimicrobial, anti-nociceptive and anti-epileptic properties, have been documented. The side effects of this herbal medicine appear not to be serious, so it can be applied in clinical trials because of its many advantages.
Conclusion
Some effects of N. sativa, such as its hypoglycemic, hypolipidemic and bronchodilatory effects, have been sufficiently studied and are sufficiently understood to allow for the next phase of clinical trials or drug developments. However, most of its other effects and applications require further clinical and animal studies.
Background & objective(s)
Global prevalence of type 2 diabetes (T2D) is very high and is currently growing alarmingly. With respect to recent researchers' attention to the potential role of herbal medicine in disease prevention and management, the present meta-analysis review investigates the effectiveness of nigella sativa (N.sativa), a popular herb, in T2D.
Methods
Literature search was conducted covering PubMed/Medline, Scopus, and Cochrane Registry of Clinical Trials up to February 2017 to obtain the relevant published intervention studies. Study selection, quality rating and data extraction of studies were investigated by two independent reviewers. Heterogeneity was assessed using I-squared (I2) statistics test. Subgroup analysis was done to assess type of N.sativa supplement as source of heterogeneity. Effect sizes of eligible studies were pooled using STATA software version 12 (STATA corp, College Station, TX, USA).
Results
Seven trials were included in the meta-analysis of glycemic and serum lipid profile end points. Supplementation with N.sativa significantly improved fasting blood sugar (FBS) [−17.84 mg/dl, 95% CI: −21.19 to −14.49, p < 0.001], HbA1c [−0.71%, 95% CI: −1.04 to −0.39, p < 0.001], total-cholesterol (TC) [WMD: −22.99 mg/dl, 95% CI: −32.16 to −13.83, p < 0.001] and LDL-cholesterol (LDL-c) [−22.38 mg/dl, 95% CI: −33.60 to −11.15, p < 0.001]. The overall effects for triglyceride (TG) [−6.80 mg/dl, 95% CI: −33.59 to 19.99, p = 0.61] and HDL-cholesterol (HDL-c) [0.37 mg/dl, 95% CI: −1.59 to 2.33, p = 0.71] were insignificant. Subgroup analysis revealed significant reduction on TG with N.sativa seed oil [−14.8 mg/dl, 95% CI: −23.1 to −6.5, p < 0.001], while TG was increased with seed powder [29.4 mg/dl, 95% CI: 16.9 to 42.0, p < 0.001]. All measures, but HbA1c, showed no evidence of publication bias.
Conclusion
Although, the meta-analysis conducted included a few number of studies, but has shown promising results on the effectiveness of N.sativa on glucose homeostasis and serum lipids. Current findings suggest N.sativa supplementation a suitable choice in managing the complications of T2D, although future researches are necessary.
Abstract:
Defective sperm function has been identified as the most common cause of infertility. The objective of this study was to review recent findings on the effects of various antioxidants on male fertility. High amounts of poly unsaturated fatty acid are found in the mammalian spermatozoa membranes, thereby making them susceptible to lipid peroxidation. Although, free radicals and reactive oxygen species (ROS) play major roles in reproduction, they are strongly associated with oxidative stress. Furthermore, factors such as obesity, inflammation, pollutants and cigarette smoking are negatively correlated with spermatogenesis. Endogenous antioxidants system exists to mediate these damages. In a normal physiological state, the seminal plasma contains antioxidant enzyme mechanism that is capable of quenching these ROS as well as protecting the spermatozoa against any likely damage. However, high level of ROS triggered by inflammatory cells and oxidation of fatty acid in obese subjects may down play antioxidant mechanism resulting in oxidative stress. Evaluation of such oxidative stress is the first step in the treatment of male infertility through administration of suitable antioxidant. Notably, antioxidant such as vitamin E and C, carotenoids and carnitine have been found beneficial in restoring a balance between ROS generation and scavenging activities. There are emerging evidences that herbal products can also boost male reproductive functions. Nonetheless, a good lifestyle, regular exercise, avoidance of stress and observing safety rules at work are habits that can reverse male infertility.
Two experiments were conducted to evaluate the effects of Nigella sativa seed and its oil added to the ration on some reproductive characteristics in broiler breeder males. In experiment I, a total
of 40 male broiler breeders (45 weeks age) were divided into five equal groups each of 8 cocks. The first group served as control; groups 2 and 3 were fed on 0.5 or 1.0% oil, while groups 3 and 4 were
fed on 0.5 or 1.0% N. sativa seed. The supplements were added daily to 135gm basal diet for 9 weeks. The main goal of this experiment was to evaluate the effect of N. sativa seed and its oil on some semen characteristics. Results showed that the addition of either seed or oil of N. sativa to the diet of the male broiler breeders resulted in the best characteristics of semen that studied in the traits. The treated groups showed an increase in ejaculation volume, sperm mass motility, progressive motility, viability percentage, count, and total sperm output. On the other hand, they exhibited a decrease in time of ejaculation and sperm abnormalities. In experiment II, a total of 200 hens and 20 cocks (55 weeks age) were randomly assigned into five equal experimental groups, each group having 4 replicates (1 0 hens and 1 cock). The hens of all five groups and cocks of first group (control) were fed on the basal ration daily for four weeks, whereas cocks of group 2, 3, 4, and 5 were fed basal ration supplemented with 0.5 or 1.0% oil or 0.5 or 1.0% seed of N. sativa, respectively. The main
goal of this experiment was to investigate the effect of N. sativa on the percentages of fertility and hatchability. Statistical analysis of the results showed significant (p˂0.05) improvement on fertility and hatchability percentages using N. sativa in the feed.
The present study provides a probable insight on the beneficial effects of medicinal plant in improving fertility and immunity of bucks. A total number of 50 male growing New Zealand rabbit were allotted and randomly divided into 5 equal groups, to study the effects of using radish, rocket and black cumin meal (at a level of 50%, respectively, as a replacement of soybean meal) and mixture of these meals at a level of 17% approximately for each, on semen characteristics, and seminal plasma and serum biochemical parameters. Each group received experimental diets containing nearly equal ratio of C/P under the same managerial conditions. The semen parameters revealed that the black cumin and the mixture diets gave the best results in case of reaction time, latency period, volume, motile sperm percentage, sperm concentration per ml, total sperm per ejaculate, total motile sperm and total function sperm fraction (8.47 vs. 7.67; 100.22 vs. 102.00; 0.79 vs. 0.79; 7.11 vs. 6.44; 82.56 vs. 87.22; 565.56 vs. 510.22; 451.44 vs. 430.22; 377.52 vs. 378.09 and 320.28 vs. 323.41 respectively). On the other hand, radish showed good results concerning motile sperm percentage, motility percentage after one hour and the resazurin reduction activity (87.50, 70.83 and 4.18 respectively). Radish meal inclusion reduced significantly (P<0.0001) the production of free radicals in seminal plasma. Nigella sativa (NS), Raphanus sativus (RS) and Eruca sativa (ES) cakes contain different type of organic compound and antioxidant. The immunogenic results for the pervious cakes improve that, the RS giving the best results form the immunity point of view followed by the mixed cake and ES cake. While the NS cake giving just higher results than control. From the present study, we concluded that the inclusion of a mixture of equal quantities from radish, rocket and black cumin meals on the expense of approximately 50% soybean meal protein improved the semen characteristics and reduced free radicals in the seminal plasma. [Magda M El-Tohamy, El-Nattat WS and El-kady RI. The Beneficial Effects of Nigella sativa, Raphanus sativus and Eruca sativa Seed Cakes to Improve Male Rabbit Fertility, Immunity and Production. Journal of American Science 2010;6(10):1247-1255]. (ISSN: 1545-1003).
This study aims to investigate the effects of Nigella sativa seeds (NSS) on fertility of male diabetic rats. Animals were divided into 3 groups, one group was kept as control and the two other were rendered diabetic by alloxan (120 mg/kg b.wt). One group was left as diabetic control (Diab) and the second were treated (Diab+N) with 2% of NSS in diet for 30 days. Blood samples were collected for glucose and testosterone levels. Testis, epididymis, prostates and seminal vesicles were removed for sperm parameters and oxidant/antioxidant status. NSS improve semen quantity and mobility, and testosterone levels and testis; they decrease blood glucose and lipid peroxidation product level (LPO) and improve antioxidant activities of glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), lactate dehydrogenase (LDH), gamma glutamyl transferase (GGT), alkaline phosphatase (ALP), and aspartate and alanine aminotransferase (AST and ALT). NNS as a diet may be beneficial for diabetic fertility.
An experiment was conducted to determine the effect of alcoholic extract of black seed Nigella sativa L. on fertility parameters in white male rats (Rattus norvegicus). A total of 60 mature males were divided into three equal groups. The first one (Control) intake drinking water, while the other two groups (T1 and T2) intake the extract in two doses (0.5 and 1.5 g/Kg, respectively) daily for 53 days. The results revealed that treatment with alcoholic extract of Nigella sativa led to significant decrease (P< 0.01) of excitation time in its three stages (1 st mount, 1 st ejaculation and 2 nd trail), significant increase (P<0.01) in body weight gain (g), reproductive parameters (seminiferous tubules thickness and diameters, account of spermatogonia, primary and secondary spermatocytes, spermatids, free spermatozoa, account of sertoli and Leydig cells, diameter of Leydig cells and the height of epithelial cells entirely covered epididymal caudal), hormones (testosterone and follicle stimulating hormone) as well as protein concentration, and significant decrease (P<0.01) in leutinizing hormone and cholesterol concentration. It could be concluded that daily oral administration of 0.5 and 1.5 g/Kg B.W of alcoholic extract of Nigella sativa L. for 53 days lead to clear improvement of male rats fertility.
Background: The task force on plants for fertility regulation in men continued with its program to identify novel prototypes in plants alleged to have fertility regulating properties. Nigella Sativa seeds are frequently used in folk medicine in the Middle East and some Asian countries for the promotion of good health and treatment of many ailments.
Objective: To evaluated the role of alcoholic extract of Nigella sativa on fertility potential, Pituitary-testicular axis hormones and Testosterone in male rats.
Materials and Methods: 24 male rats were randomly divided into 3 groups; control, group A and group B, each group comprising of 8 rats. Animals in control group received 1 ml of normal saline and treatment groups (A and B) received (gavage) graded doses of 200 and 400 mg/kg body weight of alcoholic extract of Nigella sativa seeds on a daily basis for 60 days. At the end of treatment period, fertility parameters such as body and reproductive organs weight, sperm motility, viability and count, epididymal sperm reserve (ESR), daily sperm production (DSP), blood testosterone concentration, Gonadotropins levels and fertility index were measured.
Results: There was a significant difference in testes and epididymidis weight, sperm count, ESR, DSP, blood testosterone concentration, LH and fertility index in both the lower dose group and the higher group as compared to the control group.
Conclusion: The results of this study showed that alcoholic extract of Nigella sativa seed especially in higher doses could increase fertility potential, LH and testosterone concentration in male rats.
Abstract Molecular oxygen (O 2) is essential in all species for the production of energy within mitochondria; a process known as oxidative phosphorylation. The end products of this process include adenosine triphosphate (ATP), water (H 2 O) and carbon dioxide (CO 2). In addition, very small amounts of reactive oxygen species (ROS) or free radicals are continuously produced as a consequence of normal metabolism of oxygen but which, on occasions when they become overabundant, may toxically damage cells, and therefore need to be biochemically neutralized or extruded from cells. Antioxidants are defined as substances capable of delaying or inhibiting production of ROS intermediates. Cells can either make these antioxidants endogenously, or receive them through the diet.
Therapeutic role of Nigella sativa (NS) seed oil fractions, methanolic extract (ME) and volatile oil (VO) and their constituents, thymoquinone (TQ) and limonene (LMN) in relation to lipidemic-oxidative stress in Wistar rats was determined. The total phenolic contents of NS seed oil and their ME and VO extracts were 320.00 ± 3.00, 300.12 ± 0.04 and 288.41 ± 0.01 mg gallic acid equivalents per 100 g of NS oil, respectively. Their Fe(+2) chelating activities were 870.00 ± 2.00, 222.31 ± 5.80 and 38.59 ± 1.43 mg EDTA equivalents per 100 g of NS oil, respectively. These fractions and compounds exhibited strong antioxidant activities against 2,2-diphenyl-1-picryl hydrazyl, 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, nitric oxide and hydroxyl radicals. Potential antiperoxidative effects of these fractions and compounds were also observed in liposome, and lipidemic-induced lipid peroxidation in atherogenic suspension fed rats, pretreated with 100 mg ME, 20 mg VO, 10 mg pure TQ or 200 mg LMN for 30 days. ME containing ω-6 linoleic acid and palmitic acid natural compounds was highly effective against lipidemic oxidative stress than VO extract possessing thymol and isothymol phenolic natural antioxidant compounds. TQ, principal compound shared to both the extracts. The test fractions and compounds effectively reduced the erythrocyte and liver lipid peroxidation markers, conjugated diene, lipid hydroperoxide and malondialdehyde to near normal levels in the order ME > TQ > VO > LMN, by directly counteracting free radicals as well as suppressing hepatic HMG-CoA reductase activity. Our findings demonstrated that these natural products, preferably ME possess significant antioxidant activities, and may be recommended as new potential sources of natural antioxidants.
Twenty-four Sprague-Dawley male rats (7-9 weeks old, 200-250 g) were divided into Nicotine (N) (0.5 mg/100 g body weight (BW), Nicotine Control (NC) (saline, 0.1 mL/100 g BW), Habbatus sauda oil (HS) (6.0 μ L/100 g BW), and Habbatus sauda Control (HSC) (corn oil, 0.1 mL/100 g BW) groups and treated for 100 days. Sperm parameters and seminiferous tubules measurements were evaluated. The N showed a significantly lower sperm motility (1.03 ± 0.05 × 10(6) sperm/mL) and percentage of normal (82.61 ± 0.03%) and live (93.88 ± 0.01%) sperm, higher value for the seminiferous tubule (253.36 ± 1.83 μ m) and lumen (100.15 ± 2.38 μ m) diameters and spermatogonia (19.85 ± 0.39 μ m) and spermatocytes (33.37 ± 0.59 μ m) layers, and thinner spermatid-sperm layer (22.14 ± 0.71 μ m) than the NC (P < 0.05). The HS had significantly higher sperm motility (1.49 ± 0.04 × 10(6) sperm/mL) and percentage of normal (90.61 ± 0.01%) and live (96.98 ± 0.01%) sperm, smaller lumen diameter (67.53 ± 2.34 μ m) and thinner spermatogonia (17.67 ± 0.32 μ m) and wider spermatid-sperm (36.95 ± 0.79 μ m) layers than the HSC (P < 0.05). This research confirmed that nicotine reduced sperm motility and morphology of normal and live sperms and also affected the testis histology, while Habbatus sauda oil increased sperm quality and gave better testis histological features.
Background
Nigella sativa fixed (NSFO) and essential (NSEO) oils have been used to treat diabetes mellitus and its complications. Present study was undertaken to explore and validate these folkloric uses.
Methods
Sprague dawley rats having streptozotocin (STZ) induced diabetes mellitus were used to assess the role of NSFO and NSEO in the management of diabetes complications. Parameters investigated were antioxidant potential, oxidative stress, and the immunity by in vivo experiments.
Results
The results indicated that STZ decreased the glutathione contents (25.72%), while NSFO and NSEO increased the trait significantly (P < 0.05). Experimental diets increased the tocopherol contents (P < 0.01) and enhanced the expression of hepatic enzymes (P < 0.01). Correlation matrix further indicated that antioxidant potential is positively associated (P < 0.05) responsible for the modulation of hepatic enzymes and the decrease of the nitric oxide production thus controlling the diabetes complications.
Conclusions
Overall, results of present study supported the traditional use of N. sativa and its derived products as a treatment for hyperglycemia and allied abnormalities. Moreover, N. sativa fixed and essential oils significantly ameliorate free radicals and improve antioxidant capacity thus reducing the risk of diabetic complications.
Infertility affects approximately 15% of couples trying to conceive, and a male factor contributes to roughly half of these cases. Oxidative stress (OS) has been identified as one of the many mediators of male infertility by causing sperm dysfunction. OS is a state related to increased cellular damage triggered by oxygen and oxygen-derived free radicals known as reactive oxygen species (ROS). During this process, augmented production of ROS overwhelms the body's antioxidant defenses. While small amounts of ROS are required for normal sperm functioning, disproportionate levels can negatively impact the quality of spermatozoa and impair their overall fertilizing capacity. OS has been identified as an area of great attention because ROS and their metabolites can attack DNA, lipids, and proteins; alter enzymatic systems; produce irreparable alterations; cause cell death; and ultimately, lead to a decline in the semen parameters associated with male infertility. This review highlights the mechanisms of ROS production, the physiological and pathophysiological roles of ROS in relation to the male reproductive system, and recent advances in diagnostic methods; it also explores the benefits of using antioxidants in a clinical setting.
During the past few decades, there have been numerous studies related field of free radical chemistry. Free radicals including reactive oxygen species and reactive nitrogen species are generated by the human body by various endogenous systems, exposure to different physiochemical conditions, or pathological states; and thus, they participate in the pathogenesis of increasing numbers of diseases. These free radicals are also the common by-products of many oxidative biochemical reactions in cells. In the past few years, there have been more attention and focus towards the field of free radical chemistry. If free radicals overwhelm the body's ability to regulate them, a condition known as oxidative stress ensues. Thus, they adversely alter lipids, proteins and DNA, which triggers a number of human diseases. In a number of pathophysiological conditions, the delicate equilibrium between free radical production and antioxidant capability is distorted leading to oxidative stress and increased tissue injury. Reactive oxygen species which are mainly produced by vascular cells are implicated as possible underlying pathogenic mechanisms in a progression of cardiovascular diseases including ischemic heart disease, atherosclerosis, cardiac arrhythmia, hypertension, and diabetes. This review summarizes the key roles played by free radicals in the pathogenesis of atherosclerosis, diabetes and dyslipidemia. Although not comprehensive, this review will also provide a brief perspective on some of the current research being conducted in this area for a better understanding of the role free radicals play in the pathogenesis of atherosclerosis, diabetes and dyslipidemia. This article is protected by copyright. All rights reserved.
Free radicals are well documented for playing a dual role in our body as both deleterious and beneficial species. In low/moderate concentrations free radicals are involved in normal physiological functions but excess production of free radicals or decrease in antioxidant level leads to oxidative stress. It is a harmful process that can be mediates damage to cell structures, including lipids, proteins, RNA and DNA which leads to number of diseases. A variety of synthetic medicine employed in the treatment of different diseases also capable to generate free radicals in body which may causes another disease. The plant sources are rich of antioxidants, phyto-constituents are capable to terminate free radical reactions and prevent our body from oxidative damage. Vegetables and fruits are also important sources of antioxidant substances. Different phytoconstituents and herbal product which are safer then synthetic medicine and beneficial in the treatment of diseases caused by free radicals, it also protect the body by prevent the free radicals to cause tissue injury. Phytoconstituents are conferring less side effect and compatible to body physiology. Therefore it is demand of modern era to use such phytoconstituents or phytomedicines.
Herbs are an ancient source of flavouring, aromatic compounds and medicines, not only for culinary application. The increasing interest in the powerful biological activity of plant phenolics and flavonoids outlined the necessity of determining their content in medicinal herbs. In this present study, a comparative evaluation of the polyphenol composi-tion, antioxidant capacity and biological contaminants (microbes and other organisms) as major common contaminants in medicinal herbs from the Lamiaceal family to which belong: lemon balm (Melissa officinalis), sage (Salvia officinalis) and mint (Mentha piperita) were carried out. The total phenolic and total flavonoid contents in medicinal herbs ware evalu-ated using the Folin-Ciocalteu method, were determined with an aluminum chloride colorimetric assay. The 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging effect of the herbs was measured also spectrophotometrically, like the total phenolic and total flavonoid contents. Microbiology was investigated using the current ISO methods. The present paper shows by the results of total phenolic and total flavonoid contents, antioxidant activity and biological contaminants in medicinal herbs that they must be relatively safe for the patient (consumer). Keywords: lemon balm (Melissa officinalis), sage (Salvia officinalis) and mint (Mentha piperita), total phenolics, total flavonoids, 2,2-diphenyl-1-picryl hydrazyl (DPPH), biological contaminants.
One of the major causes of defective sperm function is oxidative stress, which not only disrupts the integrity of sperm DNA but also limits the fertilizing potential of these cells as a result of collateral damage to proteins and lipids in the sperm plasma membrane. The origins of such oxidative stress appear to involve the sperm mitochondria, which have a tendency to generate high levels of superoxide anion as a prelude to entering the intrinsic apoptotic cascade. Unfortunately, these cells have very little capacity to respond to such an attack because they only possess the first enzyme in the base excision repair (BER) pathway, 8-oxoguanine glycosylase 1 (OGG1). The latter successfully creates an abasic site, but the spermatozoa cannot process the oxidative lesion further because they lack the downstream proteins (APE1, XRCC1) needed to complete the repair process. It is the responsibility of the oocyte to continue the BER pathway prior to initiation of S-phase of the first mitotic division. If a mistake is made by the oocyte at this stage of development, a mutation will be created that will be represented in every cell in the body. Such mechanisms may explain the increase in childhood cancers and other diseases observed in the offspring of males who have suffered oxidative stress in their germ line as a consequence of age, environmental or lifestyle factors. The high prevalence of oxidative DNA damage in the spermatozoa of male infertility patients may have implications for the health of children conceivedin vitro and serves as a driver for current research into the origins of free radical generation in the germ line.
Nigella sativa (N. sativa) (Family Ranunculaceae) is a widely used medicinal plant throughout the world. It is very popular in various traditional systems of medicine like Unani and Tibb, Ayurveda and Siddha. Seeds and oil have a long history of folklore usage in various systems of medicines and food. The seeds of N. sativa have been widely used in the treatment of different diseases and ailments. In Islamic literature, it is considered as one of the greatest forms of healing medicine. It has been recommended for using on regular basis in Tibb-e-Nabwi (Prophetic Medicine). It has been widely used as antihypertensive, liver tonics, diuretics, digestive, anti-diarrheal, appetite stimulant, analgesics, anti-bacterial and in skin disorders. Extensive studies on N. sativa have been carried out by various researchers and a wide spectrum of its pharmacological actions have been explored which may include antidiabetic, anticancer, immunomodulator, analgesic, antimicrobial, anti-inflammatory, spasmolytic, bronchodilator, hepato-protective, renal protective, gastro-protective, antioxidant properties, etc. Due to its miraculous power of healing, N. sativa has got the place among the top ranked evidence based herbal medicines. This is also revealed that most of the therapeutic properties of this plant are due to the presence of thymoquinone which is major bioactive component of the essential oil. The present review is an effort to provide a detailed survey of the literature on scientific researches of pharmacognostical characteristics, chemical composition and pharmacological activities of the seeds of this plant.
Summary One of the most important concerns in assisted reproduction (ART), and in particular ICSI, is the quality of sperm DNA. Oxidative stress is one of the major causes of damage to DNA and attempting to reduce generation of DNA damage related to reactive oxygen species (ROS) through consumption of antioxidants is often tempting. However, current antioxidant treatments, given irrespectively of clinically quantified deficiencies, are poorly efficient, potentially detrimental and over-exposure is risky. Here we discuss new treatments in relation to present day concepts on oxidative stress. This discussion includes stimulation of endogenous anti-ROS defense i.e. glutathione synthesis and recycling of homocysteine, the epicentre of multiple ROS-linked pathologies.
Abstract: The seeds of Nigella sativa Linn. (Ranunculaceae herbaceous plant), commonly known as black
seed or black cumin, are used as herbal medicine all over the world for the treatment and prevention of
a number of diseases and conditions like asthma, diarrhoea and dyslipidaemia. The aim of this study is
to see effects of Nigella sativa on the reproductive system and fertility on adult male albino rats. 20 albino
rats were involved in this study and were divided into two groups: a vehicle-treated control group and
a treated group with Nigella sativa at a dose of 300.mg/kg body weight for 60 days. The seeds of
Nigella sativa induce a significant increase in the weight of reproductive organs as compared to control
animals (P<0.01). The sperm motility and count in cauda epidydimides and testicular ducts were
significantly increased (P<0.01). Spermatogenesis was increased at primary &secondary spermatocyte
stages. Epididymides showed eleveted number of spermatozoa. Lumen of vas deferentia were full of
sperms. The secretary activities of seminal vesicle and ventricular prostate were also increased. A
significant increase (P<0.01) in spermatogenesis activity was observed in semniferous tubule. Treated rats
testicular cell population showed a increase in number of spermatocytes and spermatids (P<0.001) when
compared to control animals. Increased in number female rats impregnated by males receiving treatment
was also observed and (P<0.01). It is concluded that the aqueous extracts of Nigella sativa have increased
spermatogenesis of male albino rats.
Previous studies have shown that dietary supplementation with antioxidants such as vitamin E affects the reproduction parameters in heat stressed males mice Nevertheless, the impact of thymoquinone (TQ) on the reproductive system during head stress is still poorly studied. Therefore, the aim of current study was to investigate changes in the reproductive parameters during heat stress and the impact of vitamin E (positive control) and TQ during this period. Forty male mice were distributed into four groups: group I was a control group that was orally supplemented with distilled water; group 2 was subjected to HS (at a humidity of 50 to 55% and a temperature of 42°C) for 75 days; Group 3 was subjected to HS and orally supplemented with vitamin E (20 IU/kg/day for 75 days) and group 4 was subjected to HS and orally supplemented with TQ (5 mg/kg body weight/day for 75 days). We found that HS significantly increased free radicals (FR) without significant effect on the testosterone level. Additionally, semen analysis of the heat stressed mice revealed a significant decrease in sperm concentration, sperm velocity straight line (SVSL), sperm velocity curved line (SVCL), and sperm velocity average path (SVAP). Moreover, histopathological examination of seminiferous tubules of heat stressed mice presented maturation arrest in the germinal layers. Notably, supplementation with either TQ or vitamin E completely restored the FR levels, semen quality and histopathological changes that were induced by HS. Our data revealed the beneficial impacts of TQ and vitamin E supplementation in improving heat stress-induced complications.
Nigella sativa (N. sativa) seed has been an important nutritional flavoring agent and natural remedy for many ailments for centuries in ancient systems of medicine, e.g. Unani, Ayurveda, Chinese and Arabic Medicines. Many active components have been isolated from N. sativa, including thymoquinone, thymohydroquinone, dithymoquinone, thymol, carvacrol, nigellimine-N-oxide, nigellicine, nigellidine and alpha-hederin. In addition, quite a few pharmacological effects of N. sativa seed, its oil, various extracts and active components have been identified to include immune stimulation, anti-inflammation, hypoglycemic, antihypertensive, antiasthmatic, antimicrobial, antiparasitic, antioxidant and anticancer effects. Only a few authors have reviewed the medicinal properties of N. sativa and given some description of the anticancer effects. A literature search has revealed that a lot more studies have been recently carried out related to the anticancer activities of N. sativa and some of its active compounds, such as thymoquinone and alpha-hederin. Acute and chronic toxicity studies have recently confirmed the safety of N. sativa oil and its most abundant active component, thymoquinone, particularly when given orally. The present work is aimed at summarizing the extremely valuable work done by various investigators on the effects of N. sativa seed, its extracts and active principles against cancer. Those related to the underlying mechanism of action, derivatives of thymoquinone, nano thymoquinone and combinations of thymoquinone with the currently used cytotoxic drugs are of particular interest. We hope this review will encourage interested researchers to conduct further preclinical and clinical studies to evaluate the anticancer activities of N. sativa, its active constituents and their derivatives.
Oxidative stress in the male germ line is thought to affect male fertility and impact upon normal embryonic development. Accordingly, fertility specialists are actively exploring the diagnosis of such stress in spermatozoa and evaluating the possible use of antioxidants to ameliorate this condition. In this review, evidence for the presence of oxidative stress in human spermatozoa, the origins of this phenomenon, its clinical significance in the aetiology of male infertility and recent advances in methods for its diagnosis and treatment are re-examined. Moreover, an extensive review of the results presented in published clinical studies has been conducted to evaluate the overall impact of oral antioxidants on measures of sperm oxidative stress and DNA damage. Administration of antioxidants to infertile men has been assessed in numerous clinical studies with at least 20 reports highlighting its effect on measures of oxidative stress in human spermatozoa. A qualitative but detailed review of the results revealed that 19 of the 20 studies conclusively showed a significant reduction relating to some measure of oxidative stress in these cells. Strong evidence also supports improved motility, particularly in asthenospermic patients. However, of these studies, only 10 reported pregnancy-related outcomes, with 6 reporting positive associations. Adequately powered, placebo-controlled comprehensive clinical trials are now required to establish a clear role for antioxidants in the prevention of oxidative stress in the male germ line, such that the clinical utility of this form of therapy becomes established once and for all.
Existing definitions of infertility lack uniformity, rendering comparisons in prevalence between countries or over time problematic. The absence of an agreed definition also compromises clinical management and undermines the impact of research findings. The aim of this study was to perform a systematic review of the literature to determine how infertility has been defined in prevalence studies and to come up with suggestions for a feasible and clinically relevant definition.
MEDLINE, EMBASE, CINAHL and Cochrane Database of Systematic Reviews were searched for relevant population-based prevalence studies published between 1975 and 2010.
A total of 39 articles were included in the current review. The results highlight the heterogeneity of criteria used to define infertility and critical differences between demographic and epidemiological definitions. Demographers tend to define infertility as childlessness in a population of women of reproductive age, while the epidemiological definition is based on 'trying for' or 'time to' a pregnancy, generally in a population of women exposed to the risk of conception. There is considerable variation in terms of the duration of 'trying for pregnancy', the age of women sampled and their marital or cohabitation status. This leads to inconsistencies in determining the numerator and denominator used to calculate the prevalence of infertility.
There is a need for an agreed definition for infertility. We suggest a clinically relevant definition based on the duration of trying for pregnancy coupled with female age.
Various beneficial properties has been attributed to Nigella sativa, including its antioxidant potential. Previously, it was reported that supercritical fluid extraction (SFE) could be used to obtain N. sativa extract rich in antioxidants. In the present study, N. sativa extracts prepared using the previously optimized SFE as well as the traditional Soxhlet extraction approaches were analyzed for various known antioxidants. N. sativa extracts were found to prevent protein carbonyl formation as well as depletion of intracellular glutathione (GSH) in fibroblasts exposed to toluene. Furthermore, partially purified SFE and Soxhlet fractions could prevent loss of hepatic GSH in toluene-induced oxidative stressed Wistar rats as well as in L929 fibroblasts. The results showed that SFE-produced N. sativa extract is richer in antioxidants than the Soxhlet approach. It was also shown using preparative silica gel and reverse phase chromatography that different fractions of SFE-extracted or Soxhlet-extracted N. sativa had different levels of protective effects with regards to GSH depletion in vivo as well as in cell culture. Although fractions rich in thymoquinone were found to be most potent in terms of antioxidant capacity, the data indicates that the protective effects of N. sativa may not only be due to thymoquinone, but perhaps other antioxidants.
High plasma level of cholesterol or triglycerides was associated with poor semen quality and direct adverse effects on testicular function that may lead to male infertility. The effect of fixed oil of Nigella sativa (N. sativa) on male fertility in normal and hyperlipidemic rats was studied. Induction of hyperlipidemia was done by feeding rats on a diet containing 1% cholesterol, 2% sheep fat and 0.5% cholic acid for 2 months. Oil of N. sativa was orally given (0.5mL/rat) for 2 months daily to normal and hyperlipidemic rats. Plasma lipid profile including cholesterol, triglycerides, Low Density Lipoprotein (LDL) and High Density Lipoprotein (HDL) was evaluated. Fertility index as determined by sex organ weights, plasma testosterone level and sperms evaluation (sperm motility, sperm abnormalities and sperm count) was investigated. In hyperlipidemic rats (control +ve), there was a marked increase in plasma cholesterol, triglycerides and LDL levels. On the other hand, there was a significant decrease in HDL and fertility index associated with increase in sperm abnormalities. Oral administration of oil of N. sativa to either normal or hyperlipidemic rats improved the fertility index as manifested by increase in seminal vesicle weight, testosterone level, sperm motility and sperm count and a decrease in sperm abnormalities. In conclusion, administration of N. sativa oil to hyperlipidemic rats improved their reproductive efficiency and produced additional protection against hyperlipidemia induced reduction in fertility.
Patients with chronic kidney disease (CKD) experience accelerated atherosclerosis, leading to excessive cardiovascular death; oxidative stress may be a pathogenic mediator of this process. Oxidative stress is prevalent in CKD patients. Accordingly, increased oxidative stress and/or reduced antioxidant defenses have been associated with endothelial dysfunction and increased carotid intima-media thickness in CKD patients. Other studies have found a possible association between oxidative stress and a faster progression rate of kidney damage. Oxidative stress also influences the response to erythropoiesis-stimulating agents. Among possible therapeutic approaches, the use of vitamin E seems the most promising. Why antioxidant therapy is not as effective as it would be expected from the role of oxidative stress in pathogenic mechanisms remains an open question. © Springer-Verlag Berlin Heidelberg 2014. All rights are reserved.
Nigella sativa Linn. (Family-Ranunculaceae) is a widely used medicinal plant throughout India and popular in various Indigenous System of Medicine like Ayurveda, Siddha, Unani and Tibb. The seeds are used as astringent, bitter, stimulant, diuretic, emmenagogue and anthelmintic. They are also useful in jaundice, intermittent fever, dyspepsia, paralysis, piles and skin diseases. The present review is therefore, an effort to give a detailed survey of the literature on pharmacognosy, phytochemistry and pharmacological activities of the plant.
The protective effect of nigella sativa, linseed and celery oils against testicular toxicity induced by sodium valproate (SVP) in male rats was studied. The experiment was carried out along 4 weeks on fifty male rats divided into 5 equal groups. Group (1) was kept as normal control (given vehicle), while rats of group (2) were given orally SVP in a dose of 500 mg/kg /day during the last week of experimental period (positive control). The other protected three groups were pretreated by oral administration of nigella sativa, linseed and celery oils each in a dose of 250 mg/kg/day and received SVP in the last week. At the end of experiment, sex organs were obtained for semen analysis, changes in sex organs weight and for lipid peroxidation in testicular tissue and histopathology. Also blood samples were collected for serum testosterone level. Results showed that pretreatment with nigella sativa, linseed and celery oil in SVP-intoxicated rats induced significant increase in the weight of testis, sperm count and motility with a decrease in abnormal sperms. An increase in serum testosterone levels and a decrease in testicular lipid peroxides (MDA) with increase in reduced glutathione were reported in the protected rats. Histopathological examination of the testes of protected rats revealed that these oils caused alleviation of testicular degenerative lesions which seen in valporate-treated rats. In conclusion, nigella sativa, linseed and celery oils produced protective effects against testicular damage induced by SVP. This study recommends that consumption of nigella sativa, linseed or celery oils may be useful as protective agents for patients who suffer from sexual impotency.
In recent years, wide utilization of herbal drugs has encouraged scientists to determine their impressive effects on health. Since Nigella sativa L. seed (N. sativa) has many uses including infertility in traditional medicine, the effects of Nigella sativa L. seed oil on abnormal semen quality in infertile men with abnormal semen quality are of interest. This study was conducted on Iranian infertile men with inclusion criteria of abnormal sperm morphology less than 30% or sperm counts below 20 × 106/ml or type A and B motility less than 25% and 50% respectively. The patients in N. sativa oil group (n = 34) received 2.5 ml N. sativa oil and placebo group (n = 34) received 2.5 ml liquid paraffin two times a day orally for 2 months. At baseline and after 2 months, the sperm count, motility and morphology and semen volume, pH and round cells as primary outcomes were determined in both groups. Results showed that sperm count, motility and morphology and semen volume, pH and round cells were improved significantly in N. sativa oil treated group compared with placebo group after 2 months. It is concluded that daily intake of 5 ml N. sativa oil for two months improves abnormal semen quality in infertile men without any adverse effects.
DNA fragmentation is an important factor in the aetiology of male infertility. However, it is still underevaluated and its inclusion in routine semen analysis is debated. DNA fragmentation has been shown to be a robust indicator of fertility potential, more so than conventional semen parameters. Men with high DNA fragmentation levels have significantly lower odds of conceiving, naturally or through procedures such as intrauterine insemination and IVF. Couples may be counselled to proceed directly to intracytoplasmic sperm injection as it is more successful in this group, avoiding costly procedures, recurrent failures or pregnancy losses; however, this treatment is not without limitations or risks. Ideally DNA fragmentation should be minimized where possible. Oxidative stress is the major cause of DNA fragmentation in spermatozoa. Endogenous and exogenous factors that contribute to oxidative stress are discussed, and in many cases are shown to be easily modifiable. Antioxidants play a protective role, although a delicate balance of reduction and oxidation is required for essential functions, including fertilization. Reducing oxidative stress may improve a couple’s chances of conception either naturally or via assisted reproduction. Sources of oxidative stress therefore should be thoroughly examined in men with high levels of DNA fragmentation and modified where possible.
Adequate information on how leachates affect hematological and reproductive functions is necessary to help in linking causality with predictable response. The present study investigated the effects of Olushosun municipal landfill leachate (OMLL) exposure and withdrawal on sperm characteristics and erythrocytes oxidant-antioxidant balance in rats. Adult male Wistar rats were exposed to 0%, 12.5% and 25% OMLL in drinking water for 28 days. One-half of the rats in each group were sacrificed on day 29 while the remaining one-half stayed an additional 28 days without treatment. OMLL exposure significantly decreased sperm functional parameters, disrupted antioxidant systems with concomitant elevation in hydrogen peroxide and malondialdehyde levels in erythrocytes and sperm. Following withdrawal of treatment, OMLL-mediated decrease in sperm count and daily sperm production were reversed to near control. However, erythrocytes and sperm oxidative damage, increased sperm abnormalities, decreased epididymis weight, sperm progressive motility and testicular sperm number persisted and were consistent with results obtained from rats sacrificed immediately after OMLL treatment. Collectively, OMLL-induced irreversible oxidative damage to erythrocytes and sperm in rats within the time course of investigation. These findings highlight potential adverse effects of OMLL on individuals unduly exposed to leachates contaminated substances.