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The Glymphatic System - A Beginner's Guide

May 2015
Neurochemical Research [Epub ahead of print]

DOI:10.1007/s11064-015-1581-6

Authors:

Nadia Aalling

University of Copenhagen

Anne Sofie Munk

University of Copenhagen

Iben Lundgaard

Lund University

Maiken Nedergaard

University of Rochester

The glymphatic system is a recently discovered macroscopic waste clearance system that utilizes a unique system of perivascular tunnels, formed by astroglial cells, to promote efficient elimination of soluble proteins and metabolites from the central nervous system. Besides waste elimination, the glymphatic system also facilitates brain-wide distribution of several compounds, including glucose, lipids, amino acids, growth factors, and neuromodulators. Intriguingly, the glymphatic system function mainly during sleep and is largely disengaged during wakefulness. The biological need for sleep across all species may therefore reflect that the brain must enter a state of activity that enables elimination of potentially neurotoxic waste products, including β-amyloid. Since the concept of the glymphatic system is relatively new, we will here review its basic structural elements, organization, regulation, and functions. We will also discuss recent studies indicating that glymphatic function is suppressed in various diseases and that failure of glymphatic function in turn might contribute to pathology in neurodegenerative disorders, traumatic brain injury and stroke.

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ORIGINAL PAPER

The Glymphatic System: A Beginner’s Guide

Nadia Aalling Jessen

•Anne Soﬁe Finmann Munk

•Iben Lundgaard

•

Maiken Nedergaard

Received: 26 January 2015 / Revised: 6 April 2015 / Accepted: 10 April 2015

ÓSpringer Science+Business Media New York 2015

Abstract The glymphatic system is a recently discovered

macroscopic waste clearance system that utilizes a unique

system of perivascular tunnels, formed by astroglial cells,

to promote efﬁcient elimination of soluble proteins and

metabolites from the central nervous system. Besides waste

elimination, the glymphatic system also facilitates brain-

wide distribution of several compounds, including glucose,

lipids, amino acids, growth factors, and neuromodulators.

Intriguingly, the glymphatic system function mainly during

sleep and is largely disengaged during wakefulness. The

biological need for sleep across all species may therefore

reﬂect that the brain must enter a state of activity that

enables elimination of potentially neurotoxic waste prod-

ucts, including b-amyloid. Since the concept of the glym-

phatic system is relatively new, we will here review its

basic structural elements, organization, regulation, and

functions. We will also discuss recent studies indicating

that glymphatic function is suppressed in various diseases

and that failure of glymphatic function in turn might con-

tribute to pathology in neurodegenerative disorders, trau-

matic brain injury and stroke.

Keywords The glymphatic system Astrocytes 

Perivascular spaces Virchow–Robin spaces 

Cerebrospinal ﬂuid secretion Sleep Aging 

Neurodegenerative diseases Traumatic brain injury

Introduction

Clearance of excess ﬂuid and interstitial solutes is critical for

tissue homeostasis. In the peripheral tissues soluble material,

proteins and ﬂuid from the interstitial space are returned to the

general circulation by the lymphatic system [1]. The lymphatic

network extends throughout all parts of the peripheral tissues

and the density of lymph vessels correlates with the rate of

tissue metabolism. Although the brain and spinal cord are

characterized by a disproportionally high metabolic rate [2],

and synaptic transmission is exquisitely sensitive to changes in

their environment, the central nervous system (CNS) com-

pletely lacks conventional lymphatic vessels. A few older re-

ports have noted lymphatic vessel-like structures in dura, but

these channels are not lined by endothelial cells [3]. This re-

view addresses recent ﬁndings that shed light on the para-

dox that CNS lacks a lymphatic system and discusses these

ﬁndings within the broader context of what is known about

waste elimination from the CNS. Finally, we discuss our recent

ﬁndings indicating that the recently described glymphatic

system also might also serve to distribute non-waste com-

pounds such as lipids and glucose within the brain.

CSF Production

The brain consists of four ﬂuid compartments: cerebrospinal

ﬂuid (CSF), interstitial ﬂuid, intracellular ﬂuid, and the blood

vasculature (Fig. 1). The blood is kept separate from the

Nadia Aalling Jessen, Anne Soﬁe Finmann Munk and Iben Lundgaard

have contributed equally to the work.

Special Issue: In honor of Dr. Gerald Dienel.

&Nadia Aalling Jessen

Nadia_Aalling@urmc.rochester.edu

School of Medicine and Dentistry, University of Rochester

Medical Center, 601 Elmwood Ave, Box 645, Rochester,

NY 14642, USA

123

Neurochem Res

DOI 10.1007/s11064-015-1581-6

brain parenchyma and CSF by the blood–brain and blood–

CSF barriers, respectively. These barriers are essential in

maintaining the extracellular environment of the brain be-

cause they regulate the ionic and biochemical composition of

the different ﬂuid compartments [4]. The blood–brain barrier

is comprised by blood vessel endothelial cells coupled by

tight junctions, whereas the blood–CSF barrier is formed

primarily between the choroid plexus epithelial cells [5].

Because the choroid plexus capillaries, unlike other capil-

laries in the brain, are devoid of tight junctions, they are

permeable to macromolecules [4]. Choroid plexus epithelial

cells, in turn, are connected by tight junctions. At the choroid

plexus, the trans-epithelial transport of macromolecules fa-

cilitated byepithelial transporters therefore determines

which macromolecules that enter CSF from the blood

(Figs. 1,2).

In mammals, CSF comprises 10 % of the total ﬂuid vol-

ume within the cranial cavity [6]. The CSF ﬂows through the

four ventricles linked by channels or foramina into the sub-

arachnoid space of the cortex and spinal cord (Fig. 1). From

the cortical subarachnoid space it penetrates the brain

parenchyma perivascularly and bathes the brain before it

exits the CNS and drains into the lymphatic system.

CSF is thought to be produced primarily by the choroid

plexuses, which are expansions of the ependymal epithe-

lium, lining the lateral, third, and fourth ventricles [7]. The

choroid plexuses are highly folded and vascularized

structures consisting of a single layered cuboidal or low

cylindrical epithelium residing on a basement membrane.

The luminal surface area of the choroid plexus epithelial

cells is densely covered by microvilli and possess either

one primary cilia or small tufts of motile cilia [5,8].

CSF production at the choroid plexus is mediated by

exchange and transport of ions (especially Cl

,Na

and

HCO

) across the epithelial cells, which generates an

osmotic gradient that drives the movement of water from

the blood to the ventricle lumen [4,5,9]. The importance

of ionic transporters in CSF production was originally

established using pharmacological tools, which in more

recent years have been supported by genetic studies. The

Choriod plexus

CSF

Capillary

Interstitial fluid

CSF (10%)

Blood (10%)

Capillary

Astrocyte

endfeet

Blood-brain barrier

Endothelial cell

w. tight junctions

Brain parenchyma:

Interstitial uid (12-20%)

Intracellular uid (60-68%)

Lateral ventricle

Third ventricle

Fourth ventricle

Blood-CSF barrier

Choroid plexus

epithelial cell

w. tight junctions

Fenestrated

endothelial cell

Basal lamina

Pericyte

AQP4

Fig. 1 Schematic representation of the brain’s ﬂuid compartments

and barriers. The ﬂuid compartments in the brain consist of

intracellular ﬂuid (ICF) (60–68 %), interstitial ﬂuid (ISF) (also

known as extracellular ﬂuid) (12–20 %), blood (10 %) and the

cerebrospinal ﬂuid (CSF) (10 %) [6,11]. Blood is separated from the

CSF and interstitial ﬂuid by the blood brain barrier (BBB) and blood-

CSF barrier, respectively. Tight junctions between the blood

endothelial cells constitute the BBB, restricting macromolecules to

move freely from the blood into the brain parenchyma. Fluid and

solutes diffusses into the brain parenchyma from the perivascular

space located between endothelial cells and astrocytic endfeet that

expresses the water channel aquaporin-4 (AQP4). The blood-CSF

barrier is formed by tight junctions between the choroid plexus

epithelial cells. Macromolecules from the blood can move freely

between the fenestrated endothelial cells to the interstitial ﬂuid but is

restricted by tight junctions in the choroid plexus epithelial cells,

which therefore are believed to be the main players in determining

CSF composition

Neurochem Res

123

following will brieﬂy describe some of the key ionic

transport mechanisms that drive the CSF production. For

more comprehensive reviews on the role of the choroid

plexus in CSF production, we refer to [5,9].

The Na

-ATPase localized in the apical membrane

of the choroid plexus epithelial cell is central for CSF

production [5,9–11]. Data from several labs show that

ouabain, which inhibits the Na

-ATPase, reduces CSF

production by 50–60 % [10,12,13]. The Na

-ATPase

actively pumps out Na

from the epithelial cell to the CSF

in the ventricular lumen, thereby keeping the intracellular

concentration low (45 mM compared to the extra-

cellular 141–152 mM) [11]. This creates a transmembrane

gradient, which drives the import of Na

across the

basolateral membrane possibly via the Na

-dependent

HCO

co-transporter, NCBE [14] and/or the Na

exchanger NHE1 [11]. The pivotal role of Na

transport

across the choroid plexus epithelium for CSF production

has been best demonstrated in studies using amiloride, an

inhibitor of sodium transport, or by genetic depletion of

transporters in mice. Amiloride reduces CSF pro-

duction by 50 % [12,13] and brain ventricle size by 80 %

[15]. Similarly, deletion of the Slc4a10 gene encoding the

-dependent HCO

co-transporter, NCBE, decreases

the expression of the Na

-ATPase and of the water

channel AQP1 in mouse choroid plexus [16].

HCO

and its transcellular exchange with Cl

have also

been demonstrated to be important for CSF production.

Fenestrated capillary

H2O

AQP1

Na+/K+

ATPase

Na+H2O

AQP1

AE2 NCBE

Na+HCO3

Cl -

CSF-filled ventricle lumen

45 mM

148 mM

65 mM

HCO

12 mM

141 mM

152 mM

4.4 mM

102 mM

HCO

24 mM

120 mM

HCO

Basolateral

membrane

Apical

membrane

K+

Tight

junction

Cl / HCO

channel

Anion

Cl -

HCO3

NHE1

Na+

H+

Interstitial space

C.A.

CO + H O HCO + H

K+

Na+Cl-

NKCC1

Cl-

Na+

Cl-

H O

2.9 mM 22 mM

Fig. 2 Ion composition and transport across the choroid plexus

epithelial cells. According to the classical hypothesis, ion transporters

and channels in the choroid plexus epithelial cells account for the

main part of cerebrospinal ﬂuid (CSF) production. The apically

expressed Na

-ATPase, central to CSF secretion, creates the

electrochemical gradient for Na

that is imported via the Na

exchanger, NHE1 and/or the Na

–HCO

co-transporter, NCBE in

the basolateral membrane. Co-import of HCO

via NCBE and

hydration of CO

by carbonic anhydrase (C.A.) increase the

intracellular concentration of HCO

, which creates an electro-

chemical gradient that drives the efﬂux of HCO

via the basolateral-

located Cl

/HCO

exchanger, AE2 and apical HCO

channels. The

operation of AE2 generates a rise in intracellular Cl

driving the

apical export of Cl

through Cl

channels and possibly trought the

electroneutral transporter, NKCC1. It should be mentioned though

that the direction of ion transport by NKCC1 and its role in CSF

secretion is not fully understood yet. The ﬁnal result of these

processes at the choroid plexus epithelium is a net ﬂux of Na

HCO

and Cl

from the blood across the epithelium to the

ventricles, which generates the osmotic gradient that makes water

move from the blood to the ventricles across the choroid plexus

epithelium through AQP1 thereby producing the CSF

Neurochem Res

123

Application of acetazolamide or DIDS, inhibitors of carbonic

anhydrases and anion exchange, respectively, reduces CSF

formation by 30–50 % [10,17–19]. The mechanisms by

which HCO

and Cl

and their exchange contribute to

regulation of CSF production is still unclear [9]. It is

speculated that intracellular accumulation of HCO

(as a

consequence of HCO

co-import with Na

via NCBE, and

intracellular HCO

formation by carbonic anhydrase-cat-

alyzed hydration of CO

) drives the outward transport of

HCO

down its electrochemical gradient via HCO

chan-

nels and the HCO

/Cl

exchanger, AE2 in the basolateral

membrane. The exchange of HCO

with Cl

then causes

accumulation of intracellular Cl

[9,11], and generates an

electrochemical gradient for Cl

. As a result, Cl

leaves the

cell via apically-located Cl

channels and possibly via

transporters such as the electroneutral NKCC1 (that co-

transports Na

and K

to the ventricles) [9,20,21](Fig. 2).

Overall, the abovementioned processes generate a net

movement of Na

,Cl

and HCO

from the blood across the

choroid plexus epithelium to the ventricles. This outward

movement of Na

,Cl

and HCO

is believed to generate the

osmotic gradient that drives water in the same direction across

the apical membrane [5,9,11]. Water ﬂuxes across the

choroid plexus epithelium take place mainly through the

highly water permeable channel, AQP1, located primarily in

the apical membrane and to a smaller degree in the basolateral

membrane of the choroid plexus epithelial cells [20–24]. It is

debated whether AQP1 is the sole route for water transport

across the choroid plexus, however, AQP1 is critical for CSF

production since knockout of AQP1 in mice reduces the CSF

production rate by 35 % and choroid plexus water perme-

ability by 80 % compared to wildtype littermates [25,26].

Overall, the net result of ion and water movement across the

choroid plexus epithelium is production of CSF that, com-

pared to the blood, is lower in protein and K

[27], and higher

in Na

,Cl

and Mg

and has a 99 % water content com-

pared to a water content of 92 % in plasma [4,6].

Despite decades of research, surprisingly little is known

about the physiological processes regulating CSF produc-

tion. It is expected that CSF production is regulated by

intracranial pressure, but existing reports are contradictory

and suggest that intracranial pressure must be increased

signiﬁcantly or chronically to suppress CSF production [5,

28]. Additionally, CSF production might also be regulated

by the autonomic nervous system, but again the literature is

complex possibly reﬂecting the technical limitations asso-

ciated with quantifying CSF production [29].

The Choroid Plexus as the Sole Source of CSF is

Debated

CSF is continuously produced. In humans and mice CSF is

renewed approximately four and 12 times each 24 h,

respectively, and the total CSF volume of 150–160 mL in

human and 0.04 mL in mice is kept constant by removal of

CSF [4,26,30]. CSF is drained into the peripheral lym-

phatic system by efﬂux via the olfactory bulb and along

cranial and spinal nerves [21,31,32]. Recently, the im-

portance of the arachnoid granulations in CSF removal has

been questioned [33]. Hence, efﬂux along cranial and

spinal nerves and the olfactory route might represent the

most important efﬂux pathways for CSF [31,34].

According to the classical model, the choroid plexuses

alone are responsible for the vast majority (80–90 %) of

CSF formation [35–37]. Evidence for the signiﬁcant in-

volvement of the rodent choroid plexus in transport of

solutes was underscored in a proteomic study reporting that

6.7 % of the total number of proteins in the choroid plexus

is involved in transmembrane ion transport. This is a larger

proportion than in the kidney, where the proportion of

proteins estimated to be involved in ion transmembrane

transport activity was 4.8 % [38]. However, discrepancies

between experimental results from fundamental studies of

CSF formation and the classical hypothesis, have provided

the basis for a new model of CSF hydrodynamics [37,39].

Basically, it has been proposed that CSF formation occurs

by ﬁltration and ﬂux of ﬂuid through the capillary walls,

and that the respective volumes of CSF and interstitial ﬂuid

mainly depend on hydrostatic and osmotic forces between

the CSF and brain parenchyma created by gradients of

proteins and inorganic ions across the capillary membrane

[37,40]. Accordingly, under physiological conditions,

water is ﬁltered from capillaries with high capillary pres-

sure, to the interstitial ﬂuid and CSF. Since the perme-

ability of plasma electrolytes (Na

and Cl

) is low, the

electrolytes are retained. This generates an osmotic counter

pressure that opposes the water ﬁltration so when plasma

reaches the low hydrostatic pressure capillaries and

venules, water is reabsorbed into vessels from the inter-

stitial ﬂuid and CSF [37]. Hence, according to this newer

hypothesis, CSF and interstitial ﬂuid are continuously in-

terchanging and the volume occupied by each compartment

depends on hydrostatic and osmotic forces.

Recently, Buishas, Gould and Linninger introduced a

third hypothesis namely a computational model that is

based on existing empirical data. Their model attempts to

combine the two above-mentioned hypotheses and takes

into account the evidence of the glymphatic pathway [41]

explained below. The mathematical model is proposed to

‘‘predict the effects the osmolarity of ECS (extracellular

space), blood, and CSF on water ﬂux in the brain, estab-

lishing a link between osmotic imbalances and pathological

conditions such as hydrocephalus and edema’’ [41]. This

novel model needs validation, but could turn out to provide

unique insight into CSF dynamics during normal physiol-

ogy and pathology.

Neurochem Res

123

Brain Vasculature and the Perivascular Space

The brain’s vasculature has several unique features that

distinguishes it from the vasculature in the rest of the body

[42]. The arterial cerebral circulation consists of an anterior

cerebral circulation and posterior cerebral circulation sup-

plied by the internal carotid arteries and the vertebral ar-

teries, respectively. The anterior circulation, which includes

the middle and anterior cerebral arteries, communicates with

the posterior circulation, the basilar artery and posterior

cerebral arteries, via anterior and posterior communicating

arteries at the Circle of Willis [43]. From the Circle of Willis,

the anterior circulation perfuse the evolutionary younger

parts of the brain including the neocortex of the cerebral

hemispheres, while the posterior circulation supplies the

brainstem and cerebellum [43]. At the cortical surface,

cerebral arteries extend into pial arteries running through the

CSF-containing subarachnoid space and the subpial space

[44,45]. As pial arteries dive down into the brain

parenchyma they transition into penetrating arterioles

(Fig. 3)[42] and create a perivascular space, known as the

Virchow-Robin space. The Virchow-Robin spaces are ﬁlled

with CSF and bordered by a leptomeningeal cell layer on

both the inner wall facing the vessel and on the outer wall

facing perivascular astrocytic endfeet. In fact, a unique

feature of the CNS vasculature is that all arterioles, capil-

laries, and venules within the brain parenchyma are sur-

rounded by astrocytic vascular endfeet. These vascular

endfeet create the outer wall of the perivascular space re-

sembling a donut-shaped tunnel surrounding the vascula-

ture. As the penetrating arterioles narrow deeper down in the

brain parenchyma, the CSF-containing Virchow–Robin

spaces become continuous with the basal lamina. The basal

lamina is a thin sheet of loose extracellular matrix composed

primarily of laminin, ﬁbronectin, type IV collagen

and heparin sulfate proteoglycan. The endothelial cells,

pericytes and astrocytes all reside in or contact the basal

lamina. These three cells types and the other two cellular

components of ‘‘the neurovascular unit’’, smooth muscle

cells and neurons, are tightly linked to the extracellular

matrix of the basal lamina by adhesion molecules, including

integrins and dystroglycan [6,46]. Due to the loose structure

of extracellular matrix, the basal lamina provides minimal

resistance to CSF inﬂux. CSF will ﬂow from the Virchow-

Robins space down along the peri-arterial space, enter the

basal lamina that surrounds capillaries, and exit along the

perivenous space. Solutes with a molecular weight of \100

kDa will largely leave the perivascular spaces by passing

through the 50 nm clefts that separate the vascular endfeet of

astrocytes.

In addition to constituting a low resistance pathway for

inﬂux of CSF, the perivascular spaces are also important

sites for delivery of energy substrate and regulation of

blood ﬂow. In pathological conditions, such as stroke, the

innate inﬂammatory response and edema formation is ini-

tiated in the perivascular spaces [46].

From the cerebral capillaries blood continues into the

post-capillary venules where enlarged basement membranes

of endothelial cells and astrocytes provide a larger CSF-

ﬁlled perivascular space [47]. In general, blood from the

brain’s interior, including the deep white and gray matter

surrounding the lateral and third ventricles ﬂows into the

larger central/deeps veins and exits the cerebral cortex and

subcortical white matter via the cortical veins that extend to

the brain surface as pial veins [48,49]. However, in contrast

to the arterial cerebral circulation, where territories are re-

stricted to separate anterior versus posterior arteries, the

territories drained by the central veins and cortical veins

reveal a marked degree of overlapping. Hence in certain

regions, the territories of cortical veins extend far down to

the ventricular wall and in other areas, a central vein terri-

tory can include areas of the subcortical layers [48]. The

superﬁcial cortical veins anastomose with the deep veins

and empty into the superior sagittal sinus. Cerebral venous

blood from the superior sagittal sinus and the deep veins

leave the brain via a conﬂuence of sinuses draining into the

sigmoid sinuses and jugular veins [49].

The Glymphatic System

Recent studies have demonstrated that CSF and interstitial

ﬂuid (ISF) continuously interchange. This exchange is fa-

cilitated by convective inﬂux of CSF along the periarterial

space [50]. From the subarachnoid space, CSF is driven

into the Virchow-Robin spaces by a combination of arterial

pulsatility, respiration, slow vasomotion, and CSF pressure

gradients. The loose ﬁbrous matrix of the perivascular

space can be viewed as a low resistance highway for CSF

inﬂux. The subsequent transport of CSF into the dense and

complex brain parenchyma is facilitated by AQP4 water

channels expressed in a highly polarized manor in astro-

cytic endfeet that ensheathe the brain vasculature [50,51].

CSF movement into the parenchyma drives convective

interstitial ﬂuid ﬂuxes within the tissue toward the

perivenous spaces surrounding the large deep veins. The

interstitial ﬂuid is collected in the perivenous space from

where it drains out of the brain toward the cervical lym-

phatic system [31,34]. This highly polarized macroscopic

system of convective ﬂuid ﬂuxes with rapid interchange of

CSF and interstitial ﬂuid was entitled the glymphatic sys-

tem based on its similarity to the lymphatic system in the

peripheral tissue in function, and on the important role of

glial AQP4 channels in the convective ﬂuid transport.

Neurochem Res

123

In 2012, the dynamics of the glymphatic system was

characterized for the ﬁrst time in vivo using two-photon

microscopy in mice [50]. By labeling of CSF with

ﬂuorescent tracers injected into the cisterna magna, Iliff

et al. showed that CSF rapidly enters the brain along the

cortical pial arteries. The entry was followed by inﬂux

into the Virchow-Robin spaces along penetrating arteri-

oles (Fig. 4)[50]. It was evident that the CSF tracers,

rather than being diffusely and randomly distributed in the

parenchyma, entered the parenchyma through a periarte-

rial pathway surrounding the vascular smooth muscle

cells bounded by perivascular astrocytic endfeet, and

ex vivo evidence showed that tracers rapidly exited the

brain primarily along central deep veins and the lateral-

ventral caudal rhinal veins [50]. The following analyses

showed that the convective movement of periarterial CSF

into and through the brain parenchyma facilitated the

clearance of interstitial solutes to perivenous drainage

pathways, thereby extending earlier studies with labeled

proteins and small molecules (reviewed in [52]). As

proposed by Weller and colleagues [53–55], such a

macroscopic clearance mechanism of interstitial solutes

may be of particular importance for neurodegenerative

diseases including Alzheimer’s disease, which is charac-

terized by the accumulation of proteins, including amy-

loid plaques and tau tangles. To evaluate whether b-

amyloid is cleared by the glymphatic pathway, Iliff et al.

[50] injected ﬂuorescent or radiolabeled amyloid b

1–40

into the mouse striatum, and found that b-amyloid was

rapidly cleared from the mouse brain along the glym-

phatic paravenous efﬂux pathway. Furthermore, imaging

of CSF tracer movement in AQP4 knockout mice re-

vealed a *65 % reduction in CSF ﬂuid ﬂux through the

parenchyma compared to wildtype control mice and that

clearance of intrastriatal injected radio-labeled b-amyloid

was reduced by 55 % [50]. It was therefore proposed that

the paravascular glymphatic pathway driven by AQP4-

dependent bulk ﬂow constitutes a major clearance

pathway of interstitial ﬂuid solutes from the brain’s

parenchyma [51].

Glia limitans

Pial artery

Penetrating

artery

Virchow-Robin spaceSmooth muscleEndothelial cells

Artery

CSF

Basal lamina

Capillary

Arteriole

Basal lamina

AQP4

Astrocyte

Neuron

Oligodendrocyte

Pericyte

Tight junction

Endothelial

cell

Gap junction

Capillary

Leptomeningeal cells

Fig. 3 The neurovascular unit.

The structure and function of

the neurovascular unit allow

bidirectional communication

between the microvasculature

and neurons, with astrocytes

playing intermediary roles. Pial

arteries in the subarachnoid

space bathed in CSF become

penetrating arteries upon diving

into the brain parenchyma. The

perivascular space around

penetrating arteries is termed

the Virchow–Robin space. As

the penetrating arteries branch

into arterioles and capillaries,

the CSF-containing Virchow–

Robin spaces narrow and ﬁnally

disappear. CSF from the

Virchow-Robin spaces

continues its ﬂow into the

perivascular spaces around

arterioles, capillaries and

venules where the extracellular

matrix of the basal lamina

provides a continuity of the ﬂuid

space. Astrocytic vascular

endfeet expressing aquaporin-4

(AQP4) surround the entire

vasculature and form the

boundary of the perivascular

spaces

Neurochem Res

123

Lipid Transport by the Glymphatic System

The human brain weighs 2 % of the body’s total weight but

contains 25 % of the cholesterol in the human body [56].

Despite the brain being highly enriched in cholesterol, the

blood–brain-barrier prevents inﬂux of lipids and lipopro-

teins, including cholesterol, from the blood to the brain.

Unlike peripheral tissues, which obtain blood-borne

cholesterol secreted by the liver, the brain synthesizes all

its cholesterol de novo. Excess cholesterol is eliminated

from the brain by hydroxylation of cholesterol to 24-OH

cholesterol. In fact, 80 % of the 24-OH cholesterol in the

body is found in the brain and the circulatory system acts as

sink for excess cholesterol produced in the brain [57,58].

The brain is well equipped for internal lipid transport via its

own supply of lipid carrier particles of the high density

lipoprotein type secreted by astrocytes [59]. Secretion of

high density lipoprotein particles from astrocytes is de-

pendent on apolipoproteins, mainly Apolipoprotein E and

J, and allows delivery of lipids such as cholesterol to

neurons [59]. Apolipoproteins also mediate clearance of

excess hydroxylated cholesterol and b-amyloid [60], and

the Apolipoprotein E allel 4 is a major genetic risk factor

for Alzheimer’s disease [61,62]. Apolipoprotein E is

particularly concentrated in astrocytic processes at the pial

surface and around the blood vessels [63]. In addition, the

choroid plexus and tanycytes in the wall of the third ven-

tricle also produce Apolipoprotein E [63,64]. Thus,

Apolipoprotein E production is co-localized with CSF

production sites and transport pathways suggesting that

lipids are transported by the glymphatic system. Injections

of lipophilic CSF tracers showed that several different

lipophilic molecules of sizes \1 kDa, similar to the size of

cholesterol (0.387 kDa) and a larger 3 kDa lipophilic

molecule all entered the brain via periarterial routes and

exited perivenously [65], similar to hydrophilic molecules

[50]. In this way the glymphatic system was comparable to

the peripheral lymphatic system that transports the dietary

fat, incl. cholesterol, absorbed in the intestine. However,

only the \1 kDa lipophilic tracers diffused into the brain

parenchyma whereas the larger tracers were largely con-

ﬁned to the perivascular routes [65]. This suggests that the

glymphatic system plays a central role in macroscopic

distribution of lipids in the brain and that large lipid soluble

Fig. 4 In vivo two-photon imaging of glymphatic inﬂux in the mouse

cortex. aSchematic representation of the imaging setup; Periarterial

CSF inﬂux of tracers injected in the cisterna magna into the

subarachnoid CSF was assessed in vivo using two-photon microscopy

through a closed cranial window. Lower bar Imaging conducted at the

cortical surface in 1-min intervals. b5 min after injection of small

(TR-d3, dark blue) and large (FITC-d2000, green) molecular weight

tracers and c–e100 lm below the cortical surface 10, 20 and 30 min

after injection of the tracers. Merge (light blue) indicates co-

localization of TR-d3 and FITC-d2000. The cerebral vasculature

was visualized with intra-arterial ﬂourescent tracer (CB-d10), and

arteries (A) and veins (V) were identiﬁed morphologically. Immedi-

ately after intracisternal injection, CSF tracer moved along the outside

of the pial arteries, but not veins, and after 20–30 min the tracers

diffused into the brain parenchyma. Red circles arterioles, blue circles

venules. Scale bars 100 lm. Reprinted from [50] with permission

Neurochem Res

123

molecules might require speciﬁc carrier particles to be

delivered efﬁciently via the CSF. Astrocytes thus play

dual roles in lipid synthesis and lipid distribution by re-

leasing lipid carrier proteins, such as Apolipoprotein E, and

in maintaining the highway for lipid distribution, the

glymphatic system.

What Drives Glymphatic Inﬂux?

Glymphatic transport of CSF along the periarterial spaces,

followed by convective ﬂow through the brain parenchy-

ma, and exit of interstitial ﬂuid (ISF) along the perivenous

space to the cervical lymph system, is an energy requiring

process that is driven by multiple mechanisms. The con-

stant production of CSF by the choroid plexus creates a

pressure that dictates the direction of the ﬂuid ﬂow through

the ventricular system to the subarachnoid space. In addi-

tion, several lines of work show that respiration is instru-

mental in movement of CSF through the aqueduct [66,67].

Entry of CSF along the perivascular space is crucial for

facilitating glymphatic ISF–CSF exchange and clearance

function. Using reporter mice to distinguish arteries from

veins (Ds-red ﬂuorescent protein expressed under the NG2

promoter in pericytes and smooth muscle cells) [68,69], it

was demonstrated that CSF tracers follow arteries at the

pial surface running across the cortical surface and descend

along penetrating arteries, which dive perpendicularly into

the brain to reach capillary beds [50,70]. What drives the

entry of CSF along perivascular space of penetrating lep-

tomeningeal arteries speciﬁcally? Particular to arteries,

pulsation generated by smooth muscle cells creates pulse

waves along the whole length of the pial artery and

penetrating arteries diving into the brain from the cortical

surface [71–74]. Dobutamine, an adrenergic agonist, in-

creased pulsatile effect signiﬁcantly when administrated to

mice and resulted in a larger amount of CSF penetration

into the parenchyma. The opposite effect was obtained

when arterial pulsatility was dampened by internal carotid

artery ligation. Additionally, the reduction of pulse waves

decreased CSF–ISF exchange [71]. This suggests that

glymphatic activity, at least in part, is driven by arterial

pulsatility and explains why perivascular inﬂux occurs

preferentially around pulsating arteries and not cerebral

veins.

The Glymphatic System is Turned on During Sleep

While sleep is essential for all mammals, sleep is also a

vulnerable state since the decreased alertness during sleep

increase the chance of being targeted by predators. This

compromise in alertness versus rest suggests that sleep

serves a fundamental biological function. Multiple studies

indicating that sleep enhances memory consolidation,

which could be important for competition amongst species

[75–78], however, the basic biological need for sleep is

unclear [79]. Brain energy metabolism only declines by

15–25 % during sleep suggesting that sleep does not sim-

ply serve to conserve energy [80]. Recent analysis shows

that the sleep state is unique in the sense that glymphatic

activity is dramatically enhanced, while its function is

suppressed during wakefulness. In vivo 2-photon imaging

of glymphatic function showed that the CSF inﬂux in the

awake state was reduced by 90 % compared to anes-

thetized mice [81]. In order to test if this was speciﬁc to the

unconscious state or a side effect of the anesthetics used,

the same experiment was performed in naturally sleeping

mice. This analysis of CSF inﬂux showed a striking simi-

larity between true sleep and anesthetized mice. The sleep-

wake difference in glymphatic inﬂux correlated with the

volume fraction of interstitial space that was 13–15 % in

the awake state an expanded to 22–24 % in both sleep and

anesthetized mice [81]. This observation indicates that the

sleep state is particularly conducive to convective ﬂuid

ﬂuxes and thereby to clearance of metabolites. Thus, a

major function of sleep appears to be that the glymphatic

system is turned on and that the brain clears itself of

neurotoxic waste products produced during wakefulness.

The observation that glymphatic function is highly ac-

tive in both anesthetized mice and naturally sleeping mice

but not awake mice indicates that it is differences in the

sleep versus wakeful state and not daily circadian rhythms

that regulate glymphatic activity. A major driver of arousal

is the neuromodulator norepinephrine [82]. Our analysis

showed that norepinephrine also is a key regulator of

glymphatic activity and that norepinephrine might be re-

sponsible for suppression of glymphatic during wakeful-

ness. Local application of a cocktail of norepinephrine

receptor antagonists in awake mice resulted in an increase

in CSF tracer inﬂux almost comparable to that observed

during sleep or anesthesia [81]. In contrast, norepinephrine

application, mimicking the wakeful state, signiﬁcantly

decreased the interstitial volume fraction. An increase in

interstitial space volume in the sleep state reduces tissue

resistance towards convective ﬂow thus permitting CSF–

ISF exchange. Thus the burst release of norepinephrine

during arousal increases the cellular volume fraction re-

sulting in a decrease in the interstitial space [83]. In turn,

the resistance toward convective exchange of CSF and ISF

increases and this results in a suppression of glymphatic

ﬂuxes during wakefulness. Norepinephrine also acts di-

rectly on choroid plexus epithelial cells and inhibits CSF

production. Conversely, removal of norepinephrine sig-

naling, mimicking the sleep state, enhances CSF produc-

tion [84]. The concerted effect of norepinephrine thus acts

Neurochem Res

123

via different mechanisms on both ﬂuid availability and

convective ﬂuxes to suppress glymphatic function and

norepinephrine can therefore be considered both a key

regulator of the switch between the sleep and wakeful state

and solute clearance from the brain.

Convective CSF Fluxes in Aging and Pathology

Glymphatic Activity Decreases Sharply During

Aging

A recent assessment of glymphatic function in old versus

young mice showed a dramatic reduction by *80–90 % in

aged compared to young mice [85]. The suppression of

glymphatic activity included both inﬂux of CSF tracers and

clearance of radiolabeled b-amyloid and inulin. Reactive

gliosis, deﬁned by hypertrophy of GFAP

astrocyte pro-

cesses, increases with aging [86] and might contribute to

the age-related decline in glymphatic function, although

the mechanism of how changes in GFAP expression might

contribute to altered glymphatic function remains unclear.

AQP4, which in young animals is localized to astrocytic

endfeet, plays a central role in facilitating CSF–ISF ex-

change along periarterial inﬂux pathways as well as inter-

stitial solute clearance through perivascular drainage

pathways [50] (Fig. 5a). The genetic deletion of AQP4 was

previously shown to impair CSF–ISF exchange by *65 %

and reduce the clearance of b-amyloid by *55 % [50].

The lack of parity between decline in CSF–ISF exchange

and b-amyloid clearance is likely attributable to some

fraction of injected b-amyloid being cleared directly to the

blood via LRP-1/RAGE mediated trans-endothelial trans-

port [87]. However, the vascular polarization of astrocytic

AQP4 is partly lost in reactive astrocytes in old brains, i.e.

AQP4 is no longer conﬁned to astrocytic endfeet but pre-

sent in astrocytes parenchymal processes [85] (Fig. 5b).

The ﬁndings that aging was associated with loss of

perivascular AQP4 polarization, speciﬁcally along

penetrating arterioles, and that depolarization of AQP4 was

found to correlate with CSF–ISF exchange, suggests that

the age related decline in glymphatic function might be in

part attributable to dysregulation of astroglial water trans-

port. Other factors perhaps contributing to the reduction of

glymphatic activity with aging are the decline in CSF

production by 66 % and CSF pressure by 27 % percent

[71,88,89]. Aging is also accompanied by stiffening of the

arterial wall leading to a reduction in arterial pulsatility,

which is one of the drivers of glymphatic inﬂux [71,90].

The observation of age-related decline in glymphatic ac-

tivity is important because the highest risk factor identiﬁed

for neurodegenerative diseases is aging. The failure of the

glymphatic system in aging might thus contribute to the

accumulation of misfolded and hyperphosphorylated pro-

teins and thereby render the brain more vulnerable to de-

veloping a neurodegenerative pathology or perhaps

escalate the progression of cognitive dysfunction.

Indeed, all prevalent neurodegenerative diseases are

characterized by accumulation of aggregated proteins [91].

Prior work on protein degradation in CNS has primarily

focused on intracellular processes, i.e. proteasomal or

lysosomal degradation [92]. However, more recent studies

have revealed that toxic protein monomers, oligomers, and

aggregates are also present in the interstitial ﬂuid and CSF:

misfolded b-amyloid and ﬁbrillary tangles of tau in Alz-

heimer’s disease, misfolded a-synuclein in Parkinson’s

disease and misfolded superoxide dismutase in mouse

models of amyotrophic lateral sclerosis [53,93–97]. De-

posits of b-amyloid are found in hippocampal synapses

Waste

AQP4 Beta-Amyloid Neuron Astrocyte

Para-Arterial Influx Para-Venous EffluxConvective Flux

Young

Old

Alzheimer’s Disease

Metabolic

Fig. 5 Model of glymphatic function in young, old and in

Alzheimer’s disease. aIn young and healthy people, CSF enters the

brain parenchyma via periarterial pathways, washes out solutes from

the interstitial space and empties along the veins. bWith aging,

glymphatic function is reduced, possibly due to astrocytes becoming

reactive and AQP4 de-polarized from the vascular endfeet to

parenchymal processes. cIn Alzheimer’s disease perivascular space

of penetrating arteries are subject to accumulation of b-amyloid

peptides. We hypothesize that accumulation of b-amyloid might be

caused by impairment of the glymphatic system and that the

perivascular pathways are further blocked by protein aggregates such

as b-amyloid. In this model, the resulting changes in the perivascular

environment lead to abnormal enlargement of perivascular space

downstream, which further decreases glymphatic clearance

Neurochem Res

123

where activity of the neurons might lead to increased

shedding making synapses a source of extracellular b-

amyloid [98]. In concert with this, b-amyloid production is

highest during the awake state when neuronal activity is

highest [99]. However, b-amyloid is not only produced by

neurons. In fact, all cells produce b-amyloid and in par-

ticular oligodendrocytes and their precursor cells [100],

which, at least in part, might account for the myelin dis-

orders observed in Alzheimer’s disease. The emerging

concept of a prion-like spread of neurotoxic proteins ag-

gregates highlights the importance of the interstitial space

regarding deposits of aggregated protein, such as b-amy-

loid in Alzheimer’s disease [101,102]. The production and

turnover of b-amyloid is strikingly rapid in humans. In

healthy, young subjects 8.3 % of total b-amyloid is cleared

each hour via the CSF [103]. Perivascular drainage path-

ways function as a sink for interstitial b-amyloid in Alz-

heimer’s disease and perivascular spaces are a sites for

both amyloid deposits and Alzheimer’s disease pathology

[104]. Bulk clearance by the glymphatic system might in

combination with transport across the blood–brain barrier

[60] provide necessary and sufﬁcient removal of extracel-

lular b-amyloid until the end of the reproductive life span

around which time failure in adequate CSF bulk ﬂow leads

to accumulation of b-amyloid [85] (Fig. 5). This suggests

that low activity of the glymphatic system could be a major

risk factor for development of neurodegenerative diseases.

The anatomical routes of interstitial bulk ﬂow in the

perivascular space are consistent with sites of b-amyloid

accumulation, although b-amyloid accumulates pre-

dominantly at cerebral arteries. Periarterial accumulation

of b-amyloid around arteries could reﬂect recirculation of

b-amyloid-rich CSF that deposits aggregates after being

taken up by smooth muscles cells [105], although the

concept of recirculation of CSF is controversial [45]. In

turn, vascular amyloidosis might reduce glymphatic CSF

inﬂux and the stagnation of CSF inﬂux might accelerate b-

amyloid accumulation. In addition, monocytic engulfment

of b-amyloid appears to occur selectively at veins [106].

Abnormal enlargement of the perivascular space is more

frequently observed in Alzheimer’s disease compared to

aged-matched control subjects suggesting a spiral of pro-

tein accumulation, deformation of glymphatic routes and

further reduction in protein clearance and pathology

(Fig. 6).

However, abnormalities at the perivascular space are

also prominent in non-Alzheimer’s dementia. Only sur-

passed by Alzheimer’s disease, vascular dementias are the

second most common cause of dementia and these diseases

are also characterized by deformation of the perivascular

space [107,108]. Changes in or surrounding cerebral blood

vessels due to hypertension, atherosclerosis or hereditary

diseases can cause vascular dementia [108]. Vascular

dementia is often caused by pathology in small cerebral

blood vessels and capillaries, collectively termed small

vessel disease. Enlargement of the perivascular space is

frequently observed in small vessel disease [109]. An ex-

ample of this is the disease ‘‘cerebral autosomal dominant

arteriopathy with subcortical infarcts and leukoen-

cephalopathy’’ [110,111]. We speculate that these anato-

mical abnormalities in the perivascular space could have a

profound impact on glymphatic ﬂuxes due to altered phy-

sical, and perhaps also cellular signaling pathways in ways

that are non-permissive for convective ﬂuxes in the

perivascular spaces. In fact, numerous case reports found

abnormal widening of perivascular space in dementia pa-

tients otherwise in good health [112]. One explanation for

widening of the perivascular space could be periarterial

blockage leading to local obstruction of glymphatic ﬂuxes,

perhaps affecting the width of the downstream periarterial

space (Fig. 5). Roher et al. [113] hypothesized that the

enlargement of perivascular space in the white matter oc-

curs as a secondary effect of clogging of perivascular space

in the upstream artery, usually located in the grey matter.

Myelin-rich tissue may be more sensitive to obstruction of

convective interstitial ﬂuid ﬂuxes, perhaps explaining why

vascular diseases, including ‘‘cerebral autosomal dominant

arteriopathy with subcortical infarcts and leukoen-

cephalopathy’’, primarily affect white matter [114]. It is

important to note, that the potential role of glymphatic

ﬂuxes in diseases with prominent enlargement of perivas-

cular spaces has yet to be addressed experimentally, but

that gross changes of the perivascular space are likely to

negatively affect glymphatic functions. A suppression of

glymphatic function could in turn exacerbate pathology.

Traumatic Brain Injury and Its Implications

for Neurodegenerative Diseases

Traumatic brain injury, which most frequently affects

military personnel or athletes, increases the risk of prema-

ture dementia and Alzheimer’s disease [115,116]. Multiple

studies have shown that repeated traumatic events, and even

single events of moderate to severe head trauma, can lead to

progressive neurodegeneration. However, it is currently not

understood why a subpopulation of individuals develops

chronic traumatic encephalopathy, whereas other exposed

to the same degree of initial brain injury are not affected

[117]. Traumatic brain injury induces accumulation of b-

amyloid peptide and of C-tau, a proteolytically cleaved

product of MAP-tau, which is a highly abundant intracel-

lular microtubule protein in axons [118–120]. C-tau is a

biomarker of brain injury since it is released in vast quan-

tities and correlates with severity of traumatic brain injury

[121]. An emerging hypothesis is that the large amplitude

increases of interstitial tau lead to cellular uptake and

Neurochem Res

123

initiation of ﬁbrillary aggregates, which attracts additional

tau leading to formation of neuroﬁbrillary tangles ulti-

mately resulting in a prion-like spread of the pathology

[101,102]. Traumatic brain injury is linked to formation of

large astroglial scars and persistent activation of innate

neuroinﬂammation [122]. Strikingly, in a model of repeti-

tive moderate traumatic brain injury, inﬂux of CSF into the

brain and CSF-mediated clearance was impaired in the ip-

silateral hemisphere already at day 1 post injury. The re-

duction of glymphatic function persisted until at least

28 days post injury. The severe decrease in glymphatic

function was associated with glial scars characterized by

hypertrophic GFAP-positive processes in the ipsilateral

hemispheres. In addition, mis-localization of AQP4 from

vascular endfeet to parenchymal processes was observed,

similar to AQP4 mislocation noted in aging [85]. By in-

tracortical injections of human tau Iliff et al. [122] were able

to track the clearance pathway of tau. Human tau accu-

mulated around large veins and the amount of tau remaining

in the tissue correlated with a decrease in glymphatic

clearance. This suggests that CSF-mediated removal of tau

via glymphatic routes is crucial for limiting secondary

neuronal damage following traumatic brain injury. Another

study using magnetic resonance imaging (MRI) to assess

glymphatic function provided further indication that head

trauma, such as subarachnoid hemorrhage, severely impairs

glymphatic function [123]. Freshly isolated arterial blood

injected into the CSF was enough to block inﬂux pathways

of CSF into the brain, but not the cerebellum, suggesting

that cerebral hemorrhage can cause a widespread inhibition

of glymphatic function. In this model of subarachnoid

hemorrhage tissue-type application of plasminogen activa-

tor that removes ﬁbrin clots improved glymphatic perfu-

sion. Embolic ischemic stroke produced a transient

inhibition of glymphatic ﬂow in the hours following is-

chemia, however, function was spontaneously recovered at

24 h after transient ischemia [123]. This suggests that short-

term inhibition of glymphatic function, either by decreased

arterial pulsation or occlusion of perivascular pathways

caused by mild stroke, can resolve and is linked to improved

recovery.

Glymphatic Efﬂux Play a Key Role in Transport

of Biomarkers of Traumatic Brain Injury

Due to the difﬁculties in diagnosing the severity of trau-

matic brain injury based on clinical examination alone,

considerable effort has gone into developing plasma

biomarkers of brain injury. Although it is known that CSF

drains into cervical lymph nodes, prior efforts gave minute

attention to how cellular proteins, e.g. neuron-speciﬁc

enolase, GFAP and S100B, enter the blood [124]. Most

reviews have suggested that these biomarkers enter the

Fig. 6 Abnormal perivascular spaces in Alzheimer’s disease. 92.5

magniﬁcation of hematoxylin and eosin staining of superior frontal

gyrus and underlying white matter. aThe white matter of a 74-year

old with no CNS diagnosis shows homogenously stained white matter

with normal perivascular spaces. bThe white matter of an 80-year old

Alzheimer’s disease patient contains patches with paler staining and a

large number of abnormally enlarged perivascular spaces. 9100

magniﬁcation of normal (c), slightly dilated (d) and severely dilated

e) perivascular spaces in an Alzheimer’s disease patient. Reprinted

from [113] with permission

Neurochem Res

123

general circulation through opening of the blood–brain

barrier in the setting of tissue injury [125]. Instead, we

recently showed that the biomarkers of traumatic brain

injury exit the brain via the glymphatic system. Inhibition

of glymphatic activity by four mechanistically distinct

manipulations, including sleep deprivation, cisterna magna

puncture, inhibition of CSF production using acetazo-

lamide, or genetic deletion of AQP4 resulted in marked

reduction of astrocytic proteins GFAP, S100B and neuron-

speciﬁc enolase in plasma following traumatic brain injury

[126]. Several of these manipulations are clinical relevant.

For example, sleep deprivation is common in the emer-

gency room as vitals are taken frequently. Thus, plasma

biomarkers may not be a useful approach to asses the

severity of traumatic injury if it is the activity of the

glymphatic system that determines the extend by which the

biomarkers are transported from the injured brain tissue to

the blood compartment. If a patient with traumatic brain

injury is unable to sleep or if a decompressive ventricu-

lostomy is performed, the transfer of biomarkers from CSF

plasma will be low. The low level of biomarkers in the

blood under these conditions will not reﬂect the degree of

tissue injury but rather that glymphatic activity is low.

Thus, several studies have shown that acute injury, in-

cluding traumatic brain injury, subarachnoid bleeding or

stroke, profoundly impact glymphatic function and impair

convective ﬂuid ﬂow. The suppression of CSF–ISF ex-

change has immediate implications regarding the limita-

tions of the diagnostic relevance of plasma biomarkers in

traumatic brain injury [123,126]. Most importantly, im-

pairment of glymphatic function could further exacerbate

injury due to accumulation of both normal metabolic waste

as well as injury-induced debris. The degree by which

glymphatic function is suppressed after traumatic brain

injury might therefore contribute to the variability in out-

come. Future studies will deﬁne the clinical relevance of

pharmacological manipulations of the glymphatic system

after acute traumatic injury.

Prior Observations had Documented the Existence

of Perivascular Fluid Transport

Albeit, the concept of the glymphatic system was developed

just 3 years ago, many aspects of this highly organized

pathway of CSF–ISF ﬂuid exchange had already been de-

scribed. In the mid 80’s a young investigator, Patricia Grady

noted that when horseradish peroxidase, a 44 kDa glycopro-

tein, was injected in CSF, the entire vasculature of dogs or cats

was outlined by the tracer just 4–5 min later [127,128]. This

observation,which depicted widespread inﬂux of a large CSF

tracer along the perivascular space, justiﬁably received con-

siderable attention among the more established groups. Such

rapid inﬂux of CSF could not be justiﬁed by the slow process

of diffusion. Only convective ﬂuxes along the perivascular

space could explain the fast perivascular distribution of

horseradish peroxidase. However, the inability of other

groups to repeat Patricia Grady’s ﬁndings led to the incorrect

conclusion that CSF only inconsistently and to a minor extent

entered the perivascular space [129]. In retrospect, the dis-

crepancy between the observations can be explained by the

fact that Patricia Grady used larger animals that were exposed

to minimal surgery, whereas subsequent studies prepared

open cranial windows in rats, which eliminated the pressure

gradients that drive convective CSF ﬂuxes. Later, in a series of

elegant studies Gerald Dienel showed that metabolites of

glucose, including lactate, are rapidly released in large

quantities during brain activation [130,131]. Most interest-

ingly, tracers of similar molecular weights as lactate could

later be identiﬁed in the perivascular space and in cervical

lymph nodes. Thus, the work by Gerald Dienel identiﬁed the

perivascular space as a pathway for removal of metabolic

waste products. Even older studies from the group of Brad-

bury had documented that radiolabeled tracers injected into

the brain parenchyma later could be found in both the olfac-

tory bulb and the cervical lymph vessels [124].

Future Perspectives

Accumulating evidence suggests that age-related decline in

glymphatic clearance in signiﬁcant ways contributes to

accumulation of protein aggregation. Glymphatic activity

decreases by 80–90 % in old mice and several lines of

evidence have documented that b-amyloid and tau exit the

brain via the glymphatic system [85,122]. Moreover, work

from David Holtzman documents that the concentration of

b-amyloid in CSF follows the sleep-wake cycle in human

subjects [132]. It is therefore imperative that a diagnostic

test of glymphatic activity capable of identifying patients

with premature decline in glymphatic clearance is devel-

oped. Using the same logic, assessment of glymphatic ac-

tivity in patients following traumatic brain injury may

pinpoint patients with the most severe suppression of

glymphatic clearance and thereby identify the individuals

who are at higher risk for developing chronic traumatic

encephalopathy. Helene Benveniste’s group has made

headway with regard to developing a glymphatic diag-

nostic test based on MRI scans. By delivering contrast

agent into cisterna magna, the movement of CSF could be

followed in real time across the entire brain [70]. These

observations made in rats conﬁrmed and extended the no-

tion of the existence of a brain-wide glymphatic system. In

particular the MRI analysis revealed fast inﬂux kinetics at

two key nodes, the pituitary and pineal gland recesses [70].

Interestingly both of these regions are involved in

Neurochem Res

123

regulation of the sleep wake cycle [133,134]. These

studies using contrast-enhanced MRI provide the ex-

perimental groundwork for evaluating glymphatic pathway

function in the human brain and in the future assess whe-

ther failure of CSF ﬂuxes contributes to Alzheimer’s dis-

ease progression. Development of a safe and minimally

invasive imaging approach to visualize glymphatic func-

tion is therefore necessary for future translational effort. It

is in this regard important to note that subsequent analysis

showed that intrathecal lumbar injections, which are rou-

tinely used in clinical myelographic studies, provide a vi-

able alternative route to assess the basic parameters of

glymphatic function [135]. The ﬁrst studies of the glym-

phatic system in human subject is expected to be published

soon, as intrathecal administration of contrast agents al-

ready is approved for study of CSF ﬂuxes in patients un-

dergoing treatment for spontaneous intracranial

hypotension and CSF rhinorrhea [136,137].

Future studies with focus on the glymphatic system are

expected to identify functions of convective CSF ﬂuxes

beyond removal of metabolic waste products. We have

recently found that glymphatic inﬂux can serve as a dis-

tribution system for lipids [65] and glucose [138], and we

speculate that glymphatic inﬂux provides an essential route

for distribution of electrolytes, macromolecules, and other

larger compounds that enter the brain predominantly via

the blood-CSF barrier at the choroid plexus. Likewise, the

glymphatic system might serve as a path for delivery and

distribution of drugs including cancer drugs within the

brain [139]. Furthermore, we expect that growth factors

produced by the choroid plexus, as well as neuromodula-

tors released by several brain stem nuclei positioned close

to the ventricular system, are distributed widely across the

CNS by the glymphatic system. Thus, volume transmission

may, in addition to microscopic release of neuromodulators

from local nerve terminals followed by local diffusion, also

involve circulation by macroscopic convective CSF ﬂuxes

via the glymphatic system.

Acknowledgments This study was supported by NIH (NINDS

NS075177 and NS078304). We thank Gerry Dienel, Ben Kress, and

Rashid Deane for comments on the manuscript and Takahiro Takano

for illustrations.

Conﬂict of interest The authors declare that they have no conﬂict

of interest.

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Seven unique frequency profiles for scoring vigilance states in preclinical electrophysiological data

Article

Full-text available

Apr 2025

Manual scoring of longitudinal electroencephalographical (EEG) data is a slow and time-consuming process. Current advances in the application of machine-learning and artificial intelligence to EEG data are moving fast; however, there is still a need for expert raters to validate scoring of EEG data. We hypothesized that power-frequency profiles are determining the state and ‘set the framework’ for communication between neurons. Based on these assumptions, a scoring method with a set frequency profile for each vigilance state, both in sleep and awake, was developed and validated. We defined seven states of the functional brain with unique profiles in terms of frequency-power spectra, coherence, phase-amplitude coupling, α exponent, functional excitation-inhibition balance (fE/I), and aperiodic exponent. The new method requires a manual check of wake–sleep transitions and is therefore considered semi-automatic. This semi-automatic approach showed similar α exponent and fE/I when compared to traces scored manually. The new method was faster than manual scoring, and the advanced outcomes of each state were stable across datasets and epoch length. When applying the new method to the neurexin-1α (Nrxn1α) gene deficient mouse, a model of synaptic dysfunction relevant to autism spectrum disorders, several genotype differences in the 24-h distribution of vigilance states were detected. Most prominent was the decrease in slow-wave sleep when comparing wild-type mice to Nrxn1α-deficient mice. This new methodology puts forward an optimized and validated EEG analysis pipeline for the identification of translational electrophysiological biomarkers for brain disorders that are related to sleep architecture and E/I balance.

Looking for the optimal pathway to stimulate the hippocampus in human participants using transcranial ultrasound stimulation: a simulation study

Article

Full-text available

May 2025

Currently, very few methods can directly modulate neural activity in deep brain structures in human participants. Low-intensity transcranial ultrasound stimulation, an emerging and advanced modality of non-invasive brain stimulation, shows great potential for precisely stimulating subcortical structures associated with sleep, emotion, cognition, and motor function. Due to its focal nature, integrating various methods, including magnetic resonance imaging and potential pathways, is essential for performing transcranial ultrasound stimulation interventions that ensure precise targeting and dosing. The aim of this study was to create simulation models for low-intensity transcranial ultrasound stimulation targeting the human hippocampus through the lateral, anterior, and posterior pathways. We compared the effects of these models to identify the optimal pathway for transcranial ultrasound stimulation of the hippocampus. Eight healthy young adults underwent structural transcranial ultrasound stimulation scans as part of the study. Utilizing their structural neuroimaging data, we developed a protocol for low-intensity hippocampal transcranial ultrasound stimulation targeting the three pathways using the K-Plan platform, followed by acoustic and thermal simulations. The simulation results indicated that acoustic stimulation via the lateral pathway was the most effective, delivering the highest acoustic power through this route. Additionally, the geometric characteristics and thickness of the skull, along with the distance from the scalp to the cortex, significantly influenced the transcranial ultrasound stimulation dosage. These findings are a crucial step toward developing personalized treatment protocols, with lateral pathway stimulation of the hippocampus identified as the optimal option. The characteristics of the skull and the scalp-to-cortex distance are essential for accurately determining treatment targets and calculating transcranial ultrasound stimulation doses.

Relationship between cerebrospinal fluid circulation markers, brain degeneration, and cognitive impairment in cerebral amyloid angiopathy

Article

Apr 2025

Structural and functional features of brain glymphatic system: modern view

Article

May 2025
Morfologiia

Relevance. The research in the field of neuroscience confidently leads. Due to the general trends of population aging in developed countries, combined with the prolongation of active social life of people, delayed termination of professional activity, the study of the etiology, pathogenesis and treatment of neurodegenerative diseases is of particular relevance. For a long time it was believed that the brain lacks a network of lymphatic capillaries and metabolic products from the interstitial fluid of the brain parenchyma are drained into the ventricular system filled with cerebrospinal fluid. However, the discovery of a special glymphatic system of the brain [Jeffrey J. Iliff, Minghuan Wang, Yonghong Liao, Benjamin A. Plogg, Weiguo Peng, et. al.. (2012). A Paravascular Pathway Facilitates CSF Flow Through the Brain Parenchyma and the Clearance of Interstitial Solutes, Including Amyloid β. Sci. Transl. Med.. [1], allowed both neuroanatomy and clinical neuroscience to make significant progress towards understanding the nature of pathologies associated with a violation of the process of clearing the brain from metabolites. Goal. The review reflects the main scientific directions in the study of the functional morphology of the glymphatic system in the normal condition. Material and methods. The analysis of scientific literature in Russian and English in Internet databases (Google Scholar, PubMed, CyberLeninka) was carried out. Results. It has been revealed that research interest in the functional morphology of the glymphatic system is steadily growing. The article describes in detail two theories of liquor outflow based on a critical analysis of domestic and foreign literature. According to scientific data, the normal function of the glymphatic system depends on heart rate, intracranial, pulse and blood pressure levels, as well as the respiratory cycle. Moreover, the quality of sleep, the position of the head during sleep and toxic substances consumed by humans have a direct impact on the functioning of the glymphatic system. Conclusions. Further studies of the morphofunctional features of the glymphatic system in normal conditions can make significant changes in the fundamental concepts of the pathogenesis of diseases, methods of their treatment and prevention.

The Brain as a Black Body in a Black Box

Article

Full-text available

Apr 2025

The cranium houses the brain, and layers formed by the scalp, cranial bones, and meninges protect it from external energy, including light. Therefore, the brain can be viewed as a black body in a black box. The layers also protect the brain from external atmospheric or gravity force, and together with cerebrospinal fluid (CSF), it creates a microgravity or buoyant brain compartment. The brain curving (microgravity posture) mainly occurs at the mesencephalic-diencephalic junction and is thought to be because of the microgravity environment, thus, creating a nearly spherical-like brain geometry. The roughly spherical brain geometry, a black body with its heat irradiation, the presence of charged fluid in the brain extracellular space, and brain requirements to optimally cleanse waste products and be protective against microorganisms may suggest a possible presence of plasma-like brain energy. This energy has the capability to emit infrared, and its amount is determined by black-body radiation and thermodynamics principles.

Opvang van de kritiek zieke patiënt op de IC

Chapter

Oct 2024

Amplitude fluctuations of cerebrovascular oscillations and CSF movement desynchronize during NREM3 sleep

Article

May 2025
J CEREBR BLOOD F MET

Fluctuations in cerebral blood volume (CBV) are a dominant mechanism aiding cerebrospinal fluid (CSF) movement in the brain during wakefulness and non-rapid eye movement (NREM) sleep. However, it is unclear if the amplitudes of CBV oscillations also change in proportion to the changes in amplitude of CSF movement across specific NREM sleep states. It is also not known if the coupling strength between them varies between NREM sleep states. To investigate these relationships, we measured cerebral hemodynamics and craniad CSF movement at the fourth ventricle simultaneously during wakefulness and NREM sleep states using concurrent Electroencephalography and functional Magnetic Resonance Imaging. We found that the amplitude fluctuations of cerebral hemodynamics and CSF oscillations desynchronize from one another only during deep NREM3 state, despite the strong mechanical coupling between CBV changes and CSF movement, which was consistent across all states. This suggests the existence of a different mechanism, linked to the cortical interstitial volume/resistance change, that regulates the NREM3 CSF inflow into the brain.

Charged Interfaces in the Brain: How Electrostatic Forces May Guide Cerebrospinal Fluid Dynamics

Article

Full-text available

May 2025
EUR J NEUROSCI

Arturo Tozzi

Cerebrospinal fluid (CSF) flows play a main role in maintaining brain homeostasis, supporting waste clearance, nutrient delivery and interstitial solute exchange. Although current models emphasize mechanical drivers like cardiac pulsation, respiration and ciliary motion, these mechanisms alone fall short of explaining the nuanced spatiotemporal regulation of CSF flow observed under physiological and pathological conditions-even when accounting for the glymphatic framework. We hypothesize that electrostatic forces arising from charged cellular interfaces may contribute to CSF movement through electro-osmotic mechanisms. We begin by examining the biological basis for surface charge in the brain, highlighting the presence of charged glycoproteins, ion channels and dynamic membrane potentials on ependymal/glial cells interfacing directly with CSF pathways. Next, we describe key electro-osmotic principles in confined geometries, emphasizing how nanoscale surface charges can modulate fluid motion without mechanical input. Drawing from nanofluidic research, biophysics and electrohydrodynamic theory, we argue that the conditions required for electro-osmotic coupling, i.e., ionic fluid, narrow conduits and patterned surface charge, are present within brain microenvironments. To test plausibility, we present computational simulations demonstrating that surface charge patterns alone can induce structured fluid flow/solute transport, including nonlinear transitions and oscillatory behaviours that resemble physiological rhythms. These findings support the idea that electrostatics may play a modulatory role in CSF regulation, complementing mechanical drivers. By integrating different disciplines, we propose a testable, mechanistically grounded hypothesis reframing CSF dynamics as electrohydrodynamically sensitive processes. Our approach could inspire novel diagnostics/therapeutic strategies in hydrocephalus and neurodegenerative disease and inform the design of targeted drug delivery systems. Below is the unedited draft of the article that has been accepted for publication © European Journal of Neuroscience, 2025. 2 GRAPHICAL ABSTRACT This illustration visualizes the hypothesis that cerebrospinal fluid (CSF) flow within the brain may be modulated not only by mechanical forces, but also by electrostatic interactions. The image depicts CSF flowing through confined perivascular and ventricular spaces lined with glial and ependymal cells, which display patterned surface charges. These charges interact with the ionic constituents of the CSF, generating electro-osmotic forces that may drive directional or oscillatory fluid movement. The conceptual model bridges neuroscience, nanofluidics and electrohydrodynamics to propose that surface charge dynamics at cellular interfaces could contribute to localized CSF regulation, potentially influencing solute clearance, signaling and pathological states. 3

Shared Mechanisms in Neuromyelitis Optica Spectrum Disorder and Spaceflight-Associated Neuro-Ocular Syndrome: Insights into Central Nervous System Fluid Dynamics, Glymphatic Function, and Astrocyte Dysregulation

Article

May 2025

Cerebral small vessel disease: The impact of glymphopathy and sleep disorders

Article

May 2025
J CEREBR BLOOD F MET

The glymphatic system, a vital brain perivascular network for waste clearance, hinges on the functionality of the aquaporin 4 (AQP4) water channel. Alarmingly, AQP4 single nucleotide polymorphisms (SNPs) are linked to impaired glymphatic clearance, or glymphopathy, which contributes to sleep disturbances and various age-related neurodegenerative diseases. Despite the critical role of glymphopathy and sleep disturbances in cerebral small vessel disease (CSVD) – a silent precursor to age-related neurodegenerative disorders – their interplay remains underexplored. CSVD is a major cause of stroke and dementia, yet its pathogenesis is not fully understood. Emerging evidence implicates glymphopathy and sleep disorders as pivotal factors in age-related CSVD, exacerbating the condition by hindering waste removal and compromising blood-brain barrier (BBB) integrity. Advanced imaging techniques promise to enhance diagnosis and monitoring, while lifestyle modifications and personalised medicine present promising treatment avenues. This narrative review underscores the need for a multidisciplinary approach to understanding glymphopathy and sleep disorders in CSVD. By exploring their roles, emphasising the necessity for longitudinal studies, and discussing potential therapeutic interventions, this paper aims to pave the way for new research and therapeutic directions in CSVD management.

Neuroradiology (1992) 35: 1 9 D ia g n o s tic Cerebrospinal fluid flow I. Physiology of cardiac-related pulsation

Article

Full-text available

Feb 1992
NEURORADIOLOGY

Cardiac-related motion of the cerebrospinal tluid (CSF) was investigated by analysis of the velocity-dependent phase of CSF protons and flow-depcndent signal enhancement in magnitude images using ECG-gated trLASH sequences. In the cerebral aqueduct, CSF flow from the third to the fourth ventricle begins 200 ms after the R-wave of the ECG and simulates an arterial pulse wave pattern. lt lasts about 60% of the cardiac cycle and is followed by backflow from the fourth to the third ventricle, which is slower and shorter. In the spinal canal, oscillating caudad motion precedes flow from the third to the fourth ventricle by about50-100 ms and is superimposed on a bulk flow, which moves simultaneously in opposite directions in separate subarachnoid channels; it is directed mainly cau-dally in the anterior cervicalsubarachnoid space. fluid flow be used for flow-phantom measurements. trollowing the R wave of the ECG, a slice at a given anatomical position was excited 20 times, beginning with every second or third R wave (Fig. 1). Using FLASH seque nces [1 1 ] with a repetition time of 75 ms and an echo time of 10 ms. one measurement takes 1.5 s and lasts longer than one cardiac cycle (Fig. 1). Thus, all cardiac-related motion perpendicular to the excited slice during one cardiac cycle can be detected even in bradycardia, and the periodic repetition of the CSF pulsation is dircctly demonstrated on the plot of signal intensity [9]. When higher time resolution was required , repetition time was decreased to 30 ms, acknowledging the disadvantage of a measurement time shorter than one cardiac cycle.

Direct neuronal glucose uptake Heralds activity-dependent increases in cerebral metabolism

Article

Full-text available

Apr 2015

Metabolically, the brain is a highly active organ that relies almost exclusively on glucose as its energy source. According to the astrocyte-to-neuron lactate shuttle hypothesis, glucose is taken up by astrocytes and converted to lactate, which is then oxidized by neurons. Here we show, using two-photon imaging of a near-infrared 2-deoxyglucose analogue (2DG-IR), that glucose is taken up preferentially by neurons in awake behaving mice. Anaesthesia suppressed neuronal 2DG-IR uptake and sensory stimulation was associated with a sharp increase in neuronal, but not astrocytic, 2DG-IR uptake. Moreover, hexokinase, which catalyses the first enzymatic steps in glycolysis, was highly enriched in neurons compared with astrocytes, in mouse as well as in human cortex. These observations suggest that brain activity and neuronal glucose metabolism are directly linked, and identiy the neuron as the principal locus of glucose uptake as visualized by functional brain imaging.

Biomarkers of Traumatic Injury Are Transported from Brain to Blood via the Glymphatic System

Article

Full-text available

Jan 2015

The nonspecific and variable presentation of traumatic brain injury (TBI) has motivated an intense search for blood-based biomarkers that can objectively predict the severity of injury. However, it is not known how cytosolic proteins released from traumatized brain tissue reach the peripheral blood. Here we show in a murine TBI model that CSF movement through the recently characterized glymphatic pathway transports biomarkers to blood via the cervical lymphatics. Clinically relevant manipulation of glymphatic activity, including sleep deprivation and cisternotomy, suppressed or eliminated TBI-induced increases in serum S100 beta, GFAP, and neuron specific enolase. We conclude that routine TBI patient management may limit the clinical utility of blood-based biomarkers because their brain-toblood transport depends on glymphatic activity.

Drainage of cerebral interstitial fluid into deep cervical lymph of the rabbit

Article

Apr 1981

Lymph from the jugular lymph trunks of anesthetized rabbits has been continuously collected and radioiodinated albumin (RISA) therein estimated after microinjection of 1 microliter of 131I-albumin into the caudate nucleus, after single intraventricular injections, and during intraventricular infusions. Comparison of lymph at 7 and 25 h after intracerebral microinjection with efflux of radioactivity from whole brain suggests that about 50% of cleared radioactivity goes through lymph. Concentrations, normalized to cerebrospinal fluid (CSF), were much higher in lymph and retropharyngeal nodes after brain injection than after CSF injection or infusion. Also after brain injection, lymph and nodes contained more activity on injected side in contrast to lack of laterality after CSF administration. Calculation suggests that less than 30% of RISA cleared from brain can do so via a pool of well-mixed CSF. Analysis of tissues is compatible with much RISA draining by bulk flow via cerebral perivascular spaces plus passage from subarachnoid space of olfactory lobes into submucous spaces of nose and thus to lymph.

Solutes, but not cells, drain from the brain parenchyma along basement membranes of capillaries and arteries: significance for cerebral amyloid angiopathy and neuroimmunology

Article

Apr 2008

Elimination of interstitial fluid and solutes plays a role in homeostasis in the brain, but the pathways are unclear. Previous work suggests that interstitial fluid drains along the walls of arteries. Aims: to define the pathways within the walls of capillaries and arteries for drainage of fluid and solutes out of the brain. Methods: Fluorescent soluble tracers, dextran (3?kDa) and ovalbumin (40?kDa), and particulate fluospheres (0.02??m and 1.0??m in diameter) were injected into the corpus striatum of mice. Brains were examined from 5?min to 7?days by immunocytochemistry and confocal microscopy. Results: soluble tracers initially spread diffusely through brain parenchyma and then drain out of the brain along basement membranes of capillaries and arteries. Some tracer is taken up by vascular smooth muscle cells and by perivascular macrophages. No perivascular drainage was observed when dextran was injected into mouse brains following cardiac arrest. Fluospheres expand perivascular spaces between vessel walls and surrounding brain, are ingested by perivascular macrophages but do not appear to leave the brain even following an inflammatory challenge with lipopolysaccharide or kainate. Conclusions: capillary and artery basement membranes act as `lymphatics of the brain' for drainage of fluid and solutes; such drainage appears to require continued cardiac output as it ceases following cardiac arrest. This drainage pathway does not permit migration of cells from brain parenchyma to the periphery. Amyloid-? is deposited in basement membrane drainage pathways in cerebral amyloid angiopathy, and may impede elimination of amyloid-? and interstitial fluid from the brain in Alzheimer's disease. Soluble antigens, but not cells, drain from the brain by perivascular pathways. This atypical pattern of drainage may contribute to partial immune privilege of the brain and play a role in neuroimmunological diseases such as multiple sclerosis.

Neurovascular pathways to neurodegeneration in Alzheimer's disease and other disorders

Article

Jan 2011
NAT REV NEUROSCI

Berislav V. Zlokovic

Cerebrospinal fluid production in patients with hydrocephalus - Response

Article

Dec 2002
J NEUROSURG

Gerald D. Silverberg

Gene dose of Apolipoprotein E type 4 allele and the risk of Alzheimer׳s disease in late onset families

Article

Aug 1993

The apolipoprotein E type 4 allele (APOE-epsilon 4) is genetically associated with the common late onset familial and sporadic forms of Alzheimer's disease (AD). Risk for AD increased from 20% to 90% and mean age at onset decreased from 84 to 68 years with increasing number of APOE-epsilon 4 alleles in 42 families with late onset AD. Thus APOE-epsilon 4 gene dose is a major risk factor for late onset AD and, in these families, homozygosity for APOE-epsilon 4 was virtually sufficient to cause AD by age 80.

Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL)

Article

May 1997
NEUROLOGIST

BACKGROUND- Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is an inherited arterial disease of the brain. The affected gene was mapped in 1993 to chromosome 19. This mapping was essential in defining the natural history of the disease. REVIEW SUMMARY- CADASIL can start with attacks of migraine with aura occurring at a mean age of 30 years. The most frequent clinical manifestations are subcortical transient ischemic attacks or completed strokes usually observed between 40 and 50 years of age. The stroke events are sometimes associated with severe mood disturbances. The disease leads progressively to a subcortical dementia associated with pseudobulbar palsy and urinary incontinence. Mean age at death is 65 years. The severity of the clinical presentation is greatly variable between and within families. Magnetic resonance imaging (MRI) is always abnormal in symptomatic subjects. On T2-weighted images areas of increased signals in white matter and basal ganglia and on T1-weighted images, it shows a decreased signal in the same regions. Signal abnormalities can be observed a long time before the onset of clinical manifestations, as they are present in asymptomatic young family members. The severity of the MRI findings dramatically increases with age. Pathologic studies show a widespread pallor of white matter and multiple small infarcts in both the white matter and basal ganglia, associated with a small artery disease of the brain. Electron microscopy studies show that the medium of the small arteries is thickened by a granular, eosinophilic, and nonamyloid material of undetermined origin that surrounds the smooth muscle cells. These ultrastructural abnormalities have been observed in other arteries, particularly in muscular and skin arteries. The gene of CADASIL is mapped to chromosome 19 in close vicinity to the locus of familial hemiplegic migraine and to that of acetazolamide-responsive cerebellar paroxysmal ataxia. CONCLUSION- CADASIL should be suspected in subjects with a history of unexplained subcortical ischemic strokes, attacks of migraine with aura, mood disorders, or subcortical dementia whenever they are associated with MRI signal abnormalities in white matter and basal ganglia. These findings should prompt a genealogical study including all first-and second-degree relatives. CADASIL is an underdiagnosed cause of subcortical strokes.

Is There a Cerebral Lymphatic System?

Article

May 2013

The brain is unique among virtually all somatic organs in its lack of a conventional lymphatic vasculature.1–3 In the periphery, the lymphatic circulation facilitates the clearance of extracellular proteins and excess fluid from the interstitium, a role critical to tissue homeostasis and function.4,5 Yet within the brain, despite its complex architecture and high metabolic activity and neural cells’ sensitivity to changes in the extracellular environment, no specialized organ-wide anatomic structure has yet been identified that facilitates the efficient lymphatic clearance of extracellular solutes and fluid from the brain parenchyma. For small molecules and hydrophobic compounds, efflux across the blood–brain barrier is relatively unrestricted. Molecules that are substrates for specific blood–brain barrier transporters are also readily cleared from the brain.6,7 Other compounds must be cleared from the brain interstitium to the cerebrospinal fluid (CSF) compartment, where they are ultimately eliminated to the blood stream via arachnoid granulations or to peripheral lymphatics along cranial nerves.1,8,9 However, the distances between much of the brain tissue and the CSF compartments are too great for efficient clearance by simple diffusion, particularly for large molecules (such as peptides and proteins) with low diffusion coefficients.6 Rather, the clearance of these interstitial solutes from the brain is attributed to bulk flow, by which convective currents of interstitial fluid (ISF) sweep solutes along at a high rate that is largely independent of molecular size.1,2,6,7 In a controversial series of studies, Grady et al10,11 suggested that brain ISF may exchange with CSF along paravascular routes surrounding cerebral blood vessels. Because these findings seemed to be subsequently refuted by Cserr et al,12,13 such retrograde movement of CSF into the brain parenchyma is now thought to …

The Glymphatic System - A Beginner's Guide

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