Bee Pollen: Chemical Composition and Therapeutic Application

Abstract

Bee pollen is a valuable apitherapeutic product greatly appreciated by the natural medicine because of its potential medical and nutritional applications. It demonstrates a series of actions such as antifungal, antimicrobial, antiviral, anti-inflammatory, hepatoprotective, anticancer immunostimulating, and local analgesic. Its radical scavenging potential has also been reported. Beneficial properties of bee pollen and the validity for their therapeutic use in various pathological condition have been discussed in this study and with the currently known mechanisms, by which bee pollen modulates burn wound healing process.

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Review Article
Bee Pollen: Chemical Composition and Therapeutic Application
Katarzyna Komosinska-Vassev,1Pawel Olczyk,2Justyna Kafmierczak,1
Lukasz Mencner,1and Krystyna Olczyk1
1Department of Clinical Chemistry and Laboratory Diagnostics, School of Pharmacy and Division of
Laboratory Medicine in Sosnowiec, Medical University of Silesia, Jednosci 8, 41-200 Sosnowiec, Poland
2Department of Community Pharmacy, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec,
MedicalUniversityofSilesia,Kasztanowa3,41-200Sosnowiec,Poland
Correspondence should be addressed to Katarzyna Komosinska-Vassev; kvassev@sum.edu.pl
Received 29 December 2014; Revised 27 February 2015; Accepted 9 March 2015
Academic Editor: Jian-Guo Chen
Copyright © 2015 Katarzyna Komosinska-Vassev et al. is is an open access article distributed under the Creative Commons
Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is
properly cited.
Bee pollen is a valuable apitherapeutic product greatly appreciated by the natural medicine because of its potential medical
and nutritional applications. It demonstrates a series of actions such as antifungal, antimicrobial, antiviral, anti-inammatory,
hepatoprotective, anticancer immunostimulating, and local analgesic. Its radical scavenging potential has also been reported.
Benecial properties of bee pollen and the validity for their therapeutic use in various pathological condition have been discussed
in this study and with the currently known mechanisms, by which bee pollen modulates burn wound healing process.
1. Introduction
Apitherapeutics are natural agents which contain groups of
chemical compounds with approved action and range of
activity. e chemical composition of one of the most known
apitherapeutics, bee pollen, depends strongly on the plant
source and geographic origin, together with other factors
such as climatic conditions, soil type, and bees race and activi-
ties [1,2]. In the composition of the bee pollen, there are about
250 substances including amino acids, lipids (triglycerides,
phospholipids), vitamins, macro- and micronutrients, and
avonoids [1,2].
Beepollenisusedintheapitherapeutictreatmentasit
demonstrates a series of actions such as antifungal, antimi-
crobial, antiviral, anti-inammatory, immunostimulating,
and local analgesic and also facilitates the granulation process
oftheburnwoundhealing[3,4].
Beepollenisarawmaterialfromwhichbeesproducebee
bread. ey collect pollen from plant anthers, mix it with a
small dose of the secretion from salivary glands or nectar, and
place it in specic baskets (corbiculae) which are situated on
thetibiaoftheirhindlegs.esearecalledpollenloads.e
eld bees collect and transport the bee pollen to the hive [5,
6].
Inthehive,thecollectedpollen,dampenedwithsaliva
andfragmentedbyightlessbees,ispackedinhoneycomb
cells. Next, the surface of the collected pollen is covered
with a thin layer of honey and wax. e substance which
has been created is bee bread which undergoes anaerobic
fermentation and is preserved thanks to the arising lactic
acid.Beebreadconstitutesthebasicproteinsourceforthe
bee colony. Moreover, it is also the source of nutritional and
mineral substances for royal jelly produced by worker bees
[5,6].
ebeepollenoccursintheanthersofseedplantsinthe
form of 2,5–250 𝜇m grains. e grain dust is encompassed
by a double-layered cell wall. e internal cell wall is called
intine, while the external one is called exine. e exine denes
itself by a strong resistance to physicochemical factors.
Furthermore, on its surface, there are numerous pores and
furrows as well as a layer of balsam which all facilitate sticking
of pollen to bees’ abdomens [5].
Pollen grains, depending of the plant species, dier in
shape, color, size, and weight. e grain shapes are diverse:
Hindawi Publishing Corporation
Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 297425, 6 pages
http://dx.doi.org/10.1155/2015/297425

2 Evidence-Based Complementary and Alternative Medicine
round, cylindrical, bell-shaped, triangular, or thorny [7].
eir weight is equal to a dozen or several dozens of
micrograms. e majority of pollens consist of single grains
which are sometimes joined with two or more grains [7].
e color of the pollen is varied ranging from bright
yellow to black. e pollen basket, which is brought to the
hive, usually consists of the pollen from one plant. However,
it sometimes happens that the bees collect pollen from many
dierent plant species. e group of plants from which only
pollen is collected includes poppy, corn, and lupine, while
from other melliferous plants bees collect both nectar and
pollen.Beesdonotcollectpollenfromgrass.However,they
may occasionally collect fungal spores from moldy plants
[8,9].
2. Chemical Composition of Pollen
Pollen is quite a varied plant product rich in biologically
active substances. 200 substances were found in the pollen
grains from dierent plant species. In the group of basic
chemical substances, there are proteins, amino acids, car-
bohydrates, lipids and fatty acids, phenolic compounds,
enzymes, and coenzymes as well as vitamins and bioelements
[10,11].
Pollen contains 22,7% of protein on average, including
10,4% of essential amino acids such as methionine, lysine,
threonine, histidine, leucine, isoleucine, valine, phenylala-
nine, and tryptophan. ese protein elements are life essential
and the organism cannot synthesize them by itself. Moreover,
in the pollen, there are signicant amounts of nucleic acids,
especially ribonucleic one. Digestible carbohydrates occur in
thepollenintheamountof30,8%onaverage.Reducing
sugars, mainly fructose and glucose, are present in this
product in about 25,7% [12–15].
Among lipids, which are present in the pollen in the
amount of about 5,1%, the ones which should be mentioned
in the rst place are essential fatty acids (EFAs). Acids such
as linoleic, 𝛾-linoleic and archaic exist in the amount of 0,4%.
Phospholipids amount to 1,5%, while phytosterols, especially
P-sitosterol, are present in the amount of 1,1% [16].
Another group constituted phenolic compounds which
amount to 1,6% on average. is group includes avon-
oids, leukotrienes, catechins, and phenolic acids. Among
avonoids occurring in the pollen in 1,4%, there are mainly
kaempferol, quercetin, and isorhamnetin, while in the group
of phenolic acids, 0,2%, there is mainly chlorogenic acid [17].
Pollenischaracterizedbyaquitesignicantcontentof
triterpene bonds. e most frequent compounds are olean-
olic acids, 3-ursolic acid, and betulin alcohol [12,13].
Moreover, vitamins and bioelements also belong to valu-
able substances. Pollen is quite a signicant source of vitamin
both fat-soluble 0,1%, such as provitamin A and vitamins E
and D, and water-soluble 0,6%, such as B1, B2, B6, and C,
and acids: pantothenic, nicotinic and folic, biotin, rutin, and
inositol. eir total amount is equal to 0,7% in the whole
product.
Bioelements are present in about 1,6%, including
macronutrients (calcium, phosphorus, magnesium, sodium,
and potassium) and micronutrients (iron, copper, zinc,
manganese, silicon, and selenium). e latter one exists in
the amount of 0,02% [10–13].
According to the latest National Data, the average content
of main ingredients in the air-dried pollen (at the temperature
40∘C) amounts to such values as follows: proteins, 32,8%,
including essential amino acids, 11,5%, and reducing sugars,
40,7%, including sucrose, 3,7%, lipids, 12,8%, vitamin C,
0,19%, 𝛽-carotene, 0,07%, and bioelements, 4,0%.
Special devices, pollen traps, are used to collect pollen
baskets. e general rule of their functioning is to take the
part of the pollen basket from eld bees returning to the
hive. erefore, there are dierent dividers on the returning
route of bees. e bees must force their way through and,
consequently,losethepartofthepollenbasketwhichfalls
into special containers. ere are dierent types of pollen
traps: outlet, bottom-board, slice, and top-frame ones in
which perforated screens or grids with appropriately small
holes are used. eir size is about 5 mm.
e loss of pollen mobilizes the bees. It increases both the
number of eld bees and the number of ights. e amount of
pollen collected from one colony during one day amounts to
50–250 g. According to National Data, one bee colony gives 1
to 7 kg of pollen a year [12,13].
Bee bread is collected by scratching it from the combs
withaspecialforkandthenattenuatedwithwarmhoneyin
the ratio of 1 : 5. Aer leaving the mixture for several days, bee
breadfallsdownonthebottomofthecontainerasitisheavy
and separates itself from honey. Aer putting it into jars, the
product is closed tightly and stored in a cool and dark place
[18].
3. Activity and Biological Properties of Pollen
Experimental pharmacological studies, conducted on rats
andrabbits,showedthatpollenhasanhypolipidemicactivity
decreasing the content of plasma total lipids and triacyl-
glycerols. Additionally, the decrease of lipid concentration in
the serum correlated with the content of such hormones as
insulin, testosterone, and thyroxine, which are responsible for
a higher lipid metabolism [19,20].
Clinical studies conrmed the hypolipidemic activity of
pollen. It made the content of the above-mentioned lipid
substances decrease in the blood serum in patients from
20 to 35% [21]. It was also successfully applied in hyper-
lipidemia and atherosclerosis. In patients who did not react
on antiathersclerotic drug, Grobrat (fenobratum), pollen
lowered the level of lipids and cholesterol from 20 to 30% and
decreasedtheclumpingofbloodplateletsfor30%[22,23].
In patients suering from arteriosclerosis with a signicant
myopia and partial optic atrophy, pollen lowered the level of
cholesterol in blood serum and increased the eld of view and
stabilized the visual acuity [24].
Pollen and its extracts, fat-soluble ones in particular, are
successfully applied in postinfarction conditions as well as
in systemic circulation disorders and arterial hypertension.
Moreover, small doses of pollen given to older people allow
both the inhibition of the atherosclerotic changes of blood
vessels and improvement of cerebral blood ow [25].

Evidence-Based Complementary and Alternative Medicine 3
e hypoglycemic activity of pollen is mainly ascribed
tothepresenceofunsaturatedfattyacids,phospholipids,
and phytosterols. Furthermore, a decreased ability of platelet
aggregation and increased brinolytic system activity was
conrmed in people who take pollen. It indicates the
antiatherosclerotic eect which protects from heart diseases
and brain strokes [26].
e wide-ranging and well-documented studies on ani-
mals also unambiguously showed detoxifying action of
pollen. e rats were poisoned with organic solvents such
as carbon tetrachloride and trichlorethylene, as well as
ethionine and ammonium uoride, both causing a deep
damage of liver cells, and galactosamine, which imitate the
changes of viral hepatitis, ethanol, and allyl alcohol, which
induce steatosis and cirrhosis, and with drugs: paracetamol
and hydrocortisone. Under their inuence, very high levels
of enzymes such as alanine and aspartate transaminase, acid
phosphatase, and bilirubin were assayed [27]. Pollen lowered
the level of these substances in the blood serum even to
physiological values, which proves the therapeutic properties
of this product in reference to liver tissue. However, when
it was administered with toxic substances, it protected liver
cells from their harmful eect, which indicates, in turn, its
ability to prevent toxication. In the detoxifying process, an
important role is played by polyphenols, mainly avonoids
and phenolic acids [28–31].
e detoxifying activity of pollen and bee bread in
phenomena such as occupational diseases, heavy metal
contamination, industrial gases and dusts, and drugs (e.g.,
antirheumatic and anti-inammatory preparations and
antibiotics) should also be mentioned [32].
Pollen is also characterized by a high anti-inammatory
activity. Its magnitude is compared to such nonsteroidal anti-
inammatory drugs as naproxen, analgin, phenylbutazone,
or indomethacin [33].
e mechanism of anti-inammatory eect is about
inhibiting the activity of cyclooxygenase and lipoxygenase,
the enzymes responsible for turning arachidonic acid into
such toxic compounds as prostaglandin and leukotrienes,
inducing acute and chronic inammatory conditions in
tissues. e experimental research shows that a concen-
trated extract of pollen, in the dose of 50 mg for rat’s
body weight, eliminates in 75% the swelling of the given
animal’s paw induced by carrageenan administration. e
elements responsible for such activity are avonoids and
phenolic acids as well as fatty acids and phytosterols [34].
Pollen is recommended in acute and chronic inammatory
conditions, initial degenerative conditions, and cholestatic
liverdiseasesaswellasintoxicandposttraumaticdamages
of this organ [33,34].
Beepollenhasalsobeenproposedasavaluabledietary
supplement. Animal feeding experiments with pollen have
also been carried out. It was proven that mice and rats, fed
with pollen, showed a higher vitamin C and magnesium
content in thymus, heart muscle, and skeletal muscles as
well as a higher hemoglobin content and greater number of
red blood cells when compared to animals given standard
feed. Moreover, pollen also lengthened the life span of
experimental animals [35–37].
Instarvedanimalsandthosebeingonanonvitamin
diet, pollen caused faster weight gains than a normal diet.
e research proves that pollen has a high nutritional value
as well as a property of fast supplementing the nutritional
deciencies in animals’ organisms. e components playing
the vital role in the process are dispensable amino acids,
vitamins, and bioelements [36,38].
Nutritional properties of pollen and the regulating
metabolic processes are used, among others, in the cases of
children’s lack of appetite, developmental delay, and malnu-
trition of children and adults. Moreover, it is recommended
to administer pollen in the recovery period, aer surgeries,
and to people working hard physically and mentally [36,38].
Furthermore, the adaptogenic properties of pollen, which
are based on increasing the resistance to harmful physical,
chemical, and biological factors, were also indicated: it is
both (1) increasing the physical tness of the organism in
excessive physical burden, aecting the central nervous sys-
tem by improving brain functions, such as memory, learning,
comprehending, thinking, and ability to concentration, and
(2) increasing the immune system strengths against infection
en route boosting the immunological system [39].
It has been also shown that pollen ethanol extracts have
quite a strong antibiotic activity that is still being eective on
thepathogenforhumanGram-positivebacteria,forexample,
Staphylococcus aureus, and Gram-negative bacteria, includ-
ing Escherichia coli, Klebsiella pneumoniae, Pseudomonas
aeurgionsa, andonfungisuchasCandida albicans.e
responsibility for this activity lies in avonoids and phenolic
acids [40,41].
Recent research indicates that pollen has an antialler-
gic activity. It protects mast cells of the organisms from
degranulation that is from releasing histamine which is
the exponent of allergic reactions. For instance, releasing
histamine from mast cells, induced by the serum containing
anti-IgE antibodies, was inhibited by pollen in 62% [42].
e literary data point out that pollen seals capillaries,
removes swellings of cardiovascular and renal origin, and has
a spasmolytic eect on smooth muscles especially in the range
of bladder and urethra [43].
e benecial eect of pollen in inammatory conditions
of prostate gland is known for a long time. Clinicians
conrm that, in nonbacterial prostate inammations, pollen
improves the condition of patients eectively removing the
pain. e positive eect was found in benign prostatic
hyperplasia cases. In the initial stage of the prostate cancer,
theimprovementwasalsofound.However,whenpollen
was administered alongside chemotherapeutic agents, the
number of people who felt a signicant therapeutic eect
signicantly increased [44–47].
Pollen, administered alongside antidepressants, enables
the lowering of their doses and improves the overall condition
in a short period of time. Due to this fact, there are fewer cases
of drug addictions or occurrences of side eects. Owing to
its nutritional and tonic properties as well as improvement of
blood supply to nervous tissue, pollen boosts mental capacity
and strengthens the nervous system weakened by stress or
overworking [48–50]. erefore, pollen is eective in treating
physical and mental overtiredness, asthenia, and apathy.

4 Evidence-Based Complementary and Alternative Medicine
Particularly good eects are gained in depressions caused
by decreased life energy, especially in older people. Long-
term use of pollen, even in small doses, enables gradual mood
improvement, restores the desire to live, and strengthens the
organism physically [50].
Good results of treatment with pollen and bee bread were
gained in geriatrics in the symptoms of early old age as well
as in neurasthenic inertia in older people. Pollen is a vital
element in treating chronic alcoholic disease. Small doses of
pollen and tranquilizers together with uid administration
enable both the alleviation of the abstinence symptoms
and signicant reduction of their duration. Deciencies of
many substances such as proteins, vitamins, and bioelements,
magnesium in particular, which occur in chronic alcoholism,
are supplemented to a great extent by pollen [17,51].
4. Routes of Administration and Dosing
In adults, 20–40 g is applied therapeutically every day. If a
teaspoon is 7,5 g of pollen, it can be concluded that one dose
is 3–5 teaspoons of this product for adults and 1-2 teaspoons
for children. Pollen is usually taken 3 times a day before
eating.etimeoftreatmentis1–3months,butitcanbe
repeated 2–4 times a year. e most appropriate period for
treatment is between winter and spring and between summer
and autumn. Generally, a smaller dose of pollen is used in
the combination therapy, alongside other medicaments and
in chronic diseases [52].
Bee bread, as a product of a stronger action than pollen, is
usually administered in smaller amounts or for a short period
of time. Romanian researchers, in the therapy of a chronic
hepatitis, gained the same results for bee bread used in the
amount of 30 g daily during a month and for pollen in the
exactly the same dose administered for 3 months.
In order to increase the digestibility of the organism,
pollen grains are shredded by grinding or are subjected
to warm water. In the water environment, pollen grains
become swollen and, aer 2-3 hours, crack and, consequently,
release their values. Milk, fruit, and vegetable juices are also
used for this purpose. (Ground) pollen may be mixed with
many products in the ratio from 1 :1 to 1 : 4 with the use
of honey, butter, cottage cheese, yoghurt, jams, glucose, and
others. Mixed pollen is taken in the amount of 1 teaspoon 3
times a day. In many diseases, however, enzymatic pollen is
recommended for use.
To sum up, it should be emphasized that unshredded
pollen, accurately chewed before swallowing, is used by the
organism only in about 10–15%. Aer mechanical shred-
ding or natural release, the accessibility of biological pollen
increases to 60–80% [52,53].
5.PolleninBurnWoundsTreatment
Apitherapy is becoming more and more recognized among
contemporary and conventional treatment methods as it uses
therapeutic eect of standardized, pharmacologically active
fractions obtained from bee products. Literary data indicate
that the antioxidating, immunomodulating, epithelialization
accelerating properties and bacteriostatic and anesthetic
characteristics and the advisability of its application in burn
wound treatment are conrmed [54,55]. Furthermore, the
equally important fact is that pollen has a strong anti-
inammatory eect, shortens the healing time, decreases the
discomfort of both the duration period and the intensity
of ailments, and is denitely less costly. e mechanism
of the inammatory eect is about inhibiting the activity
of enzymes which are responsible for the development of
inammatory process mediators in tissues. Flavonoids and
phenolic acids are mainly responsible for such actions, but
fatty acids and phytosterols also take part in this process
[33,34].
What is more, kaempferol, which is included in pollen,
thanks to its ability to inhibit the activity of two enzymes:
hyaluronidase, which is the enzyme catalyzing depolymeri-
sation of hyaluronic acid, and elastase, which hydrolyses
elastin, strengthens the connective tissue and seals blood
vessels. is results in decreased transudates, inammatory
reactions, and swellings. Blood circulations in the vessels
improve and, therefore, skin becomes moistened and tight.
e antiedematous, anti-inammatory, and analgesic action
of avonoids may also result from a dierent compound
bioactivity; for example, quercetin, by inhibiting the activity
of histidine decarboxylase, lowers the histamine level in the
organism. Moreover, inhibiting the cascade of arachidonic
acid metabolism, which in turn lowers the level of proin-
ammatory prostaglandins and gives the anti-inammatory
eect, removes local pain and prevents platelet aggregation
[52,56–58].
It should also be mentioned that one of the factors
interrupting the healing process of wounds is infection.
Particularly susceptible to infections are postburn wounds,
which were the subject of previous studies on experimental
therapy of burns with propolis. Extensive burns are the gates
of infection for many microorganisms, while the necrotic
tissues are a very good environment for such microorganisms
to develop [54]. e therapeutic mechanism of apitherapeu-
tics is based, among others, on antimicrobial activity and on
inducing processes of regenerationof damaged tissues. ese
properties indicate the possibility of using apitherapeutics in
burn wound treatment and ulcerations of dierent etiology
[40,59]. e conducted studies, that have not been published
yet, prove that the ointment with bee pollen extract has
an antimicrobial activity regarding the bacterial ora of
postburn wounds. Moreover, the apitherapeutic method of
burn wound treatment, including topical application of the
bee pollen ointment, is additionally deprived of undesirable
eects and is alternative to topical burn wound treatment.
Conflict of Interests
e authors declare that there is no conict of interests
regarding the publication of this paper.
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