Background and Purpose: Myostatin and follistatin are key proteins in muscle tissue regulation, growth,
and hypertrophy. This study aimed to investigate the effects of two types of resistance training on the serum
levels of myostatin and follistatin and the ratio of follistatin to myostatin in healthy young men.
Materials and Methods: Twenty active young men were randomly divided into two equal groups of
pyramid resistance exercises (, Mean±SD; age, 22.3±4.7 years; body mass index, 23.1±3.9 kg/m2; fat
percentage, 27.6±10.7) and inverted pyramid resistance exercises (Mean±SD; age, 21.11±3.55 years; body
mass index, 23.2±6.7 kg/m2; fat percentage, 26.3±13.8). The training lasted for eight weeks and three
sessions per week. Each training session included six movements of chest press, leg press, barbell curl,
triceps extensions, leg flexions, and leg extensions at an intensity corresponding to 100-50% of one
maximum repetition in the pyramid resistance training group and at an intensity of 50-100% of one
maximum repetition in the inverted pyramid resistance training group. Data analyses were carried out by
using dependent t-test and ANCOVA at a significance level of P≤ 0.05.
Results: Data analyses showed that eight weeks of pyramid and inverted pyramid resistance training caused
a significant decrease in the serum level of myostatin (P=0.004,P=0.01, respectively) and a significant increase
in the serum level of follistatin (p = 0.001, P=001), and the ratio of follistatin to myostatin (P=0.001, P=0.001)
in healthy men. The results for ANCOVA analyses showed that there is no significant difference between the
two groups for serum levels of myostatin, follistatin, and the ratio of follistatin to myostatin (p>0.05).
Conclusion: Pyramid and reverse pyramid resistance training were associated with an increase in
follistatin, the ratio of follistatin to myostatin, and a decrease in myostatin. Since, we found no significant
difference between the experimental groups, it could be concluded that in young men these training
protocols may lead to muscular hypertrophy similarly.
Keywords: Resistance Training, Pyramid Resistance Training, Inverted Pyramid Resistance Training,
Myostatin, Follistatin
How to cite this article: Cherag Birjandi S, Farhadpour M. Comparison of the effects of two types of
resistance training on the serum levels of factors involved in muscular hypertrophy in healthy young men. J
Sport Exerc Physiol. 2024; 17(2):67-79