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Histological Studies Of The Effects Of Oral Administration Of Damiana (Turnera Diffusa) On The Liver Of Mature Wistar Rats
Abstract
Histological studies of the effect of oral administration of Damiana extract on the liver of mature wistar rats was carried out at the Department of Anatomy, University of Ilorin, Kwara State between December 2002 and July 2003. This study involved the oral administration of 0.52mg Damiana extract daily on various days. The experimental animals were randomly divided into four groups (A, B, C, D) of five each with the D as control. The rats in group A received 0.52mg in 1ml of Damiana extract for a day, those in group B 0.52mg Damiana extract in 1ml daily for a period of five days, while those in group C got 0.52mg/ml Damiana for ten days. The animals were sacrificed at the end of each experiment and the liver sections were obtained for routine histological investigation. Histological changes observed in the liver sections included dilatation of the central veins, cyto-architectural distortions of the hepatocytes, vacuolations, sparse and scattered sinusoids in the treated liver sections. Our results suggest that the liver functions could also have been adversely affected due to the distortion of the cyto-architecture and sparse and scattered sinusoid of the liver. It is recommended that further studies aimed at corroborating these observations be carried out.
... According to a study, when damiana(Tunera diffusa) was administered to adult Wistar rats, it caused reduced size and number of renal corpuscles, distortion of renal structures and some degree of necrosis in kidney histology. 16 Hence the results of this study were in concordance with the previous work.The findings implicated that carbonated beverages were capable of precipitating kidney disease most probably by causing congestion and tubular necrosis of kidney. According to a study accidental or pathologic cell death was regarded necrotic, which could result from an extrinsic insult to cells as a thermal, osmotic, toxic or traumatic effect. ...
Background: To study the histologic effects of carbonated drinks on rat kidneys Methods: In this experimental animal study histological changes on rat kidney caused by chronic consumption of carbonated drinks were observed. Thirty adult Wistar rats with average weight of 200g were randomly assigned into three groups, ten per group . Group A and B served as treatment groups while group C served as the control. The rats in group A were administered a randomly selected carbonated drink ad libitum for thirty days. Rats in group B were given ad libitum access to carbonated drink for sixty days and animals in group C were given water. The rats in group A and B were sacrificed in CO2 and Chloroform chamber on day 30 and 60 respectively. The abdominal region was quickly opened and the kidney dissected out and fixed in 10% formal saline. Deparaffinized sections were stained routinely with haematoxylin and eosin (H & E).
Results: H and E stain study revealed glomeruli congestion and inflammatory cell infiltrate in group A specimens. The specimens of group B showed glomeruli congestion along with tubular necrosis and nuclear cell disturbance.
Conclusion: Long term consumption of carbonated drinks adversely affects histology of kidney, and the deterioration of cells increases with increase in the consumption of beverage.
... The histological effects observed in this experiment is in consonance with the report of Manjrekar et al. who observed that P. amarus induced deleterious changes on the renal tubules and testes of male rats (12,13). It is also in consonance with the reported effects of damiana (Turnera diffusa) on matured Wistar rats where distortion of the renal cortical structures, reduced number and size of the renal corpuscles were observed (15). ...
Phyllanthus amarus is commonly used for treatment such as in gastro, urogenital diseases and infection. However, it is speculated to have some toxic effects such as renal tubular damage.
This study was to investigate the histological effects of chronic administration of the herb on kidney of adult Wistar rats.
Rats of both sexes (n = 24), with average weight of 200g were randomly assigned into two treatments (A and B) and control (C) groups of 8 rats each. Rats in treatment groups (A) and (B) respectively received daily administration of 400mg and 800mg of aqueous Phyllanthus amarus, per 70kg body weight for 30days through the orogastric tube. The control group received distilled water through the same route. All rats were fed with grower's mash and given water liberally. The rats were sacrificed by cervical dislocation on the thirty-first day of the experiment and the kidneys were carefully dissected out and quickly fixed in 10% formal saline for histological study.
The observations indicate that rats in the treated groups showed some varying degree of distortion and disruption in microanatomy of the kidney including interstitial oedema and tubular necrosis, when compared to the control section.
This report provides further evidence that medicinal use of Phyllanthus amarus has a potential adverse effect. This warrants further studies to establish or rule out any untoward side-effect of chronic renal dysfunctions.
... They reported that administration of camphor resulted in mild edema with glomerulonephritis, glomerular lobulation, tubular necrosis and congestion of blood cell in the kidney of rabbit[10]. Administration of damiana (Turnera diffusa) to matured Wistar rats resulted in the distortion of the renal cortical structures, reduced size and number of the renal corpuscles and some degree of cellular necrosis in the histology of the kidney[11]. ...
Health concerns over soda pop drinks have been severally report. However, histological perspectives are not very common.
The objective of this study is to investigate histological effect of chronic consumption of soda pop drinks on the kidney of adult Wistar rats.
The rats of both sexes (n = 24), with average weight of 200g were randomly assigned into two treatment (A & B) (n=16) and Control (c) (n=8) groups. The rats in the treatment group (A) received a brand of soda pop drink on a daily basis for thirty days. The rats in treatment group (B) received another brand of soda drink, while the control group (C) received equal amount of water for the same period. The rats were given the drinks as well as feeds liberally for thirty days, and sacrificed by cervical dislocation on the thirty-first day of the experiment. The kidney was carefully dissected out and quickly fixed in 10% formal saline for histological study.
The findings indicate that rats in the treated groups (A&B) showed some varying degree of distortion and disruption of the renal structure. There are observable diffuse signs of glomerulonephritis with some congestion and tubular necrosis as compared to the control group.
Chronic consumption of soda pop drinks may affect the microanatomy of the kidney of adult Wistar rats. Further study aimed at corroborating these observations in humans is warranted.
Efavirenz is commonly used as part of the highly active antiretroviral therapy (HAART) for the treatment of human immunodeficiency virus (HIV) type 1. The effects of chronic administration of efavirenz on the kidney of adult Wistar rats were investigated. The rats were divided into two groups, those given efavirenz (treatment) and those given water (control), both for 30 days. The animals were sacrificed by cervical dislocation on Day 31 of the experiment and kidneys were dissected, weighed and quickly fixed in 10% formalin for histological analysis. The findings indicate that there was a significant (p < 0.05) decrease in weight and increase in relative weight of treated right and left kidneys compared to the control group. Kidneys in the treated group showed disruption of the cytoarchitecture of the renal cortical structure, diffuse glomerulonephritis with some cell congestion, and dilation of Bowman’s space compared to the control group. Chronic efavirenz administration may therefore have an adverse effect on the kidney function of adult Wistar rats. Further studies to corroborate these observations should be carried out.
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