Currently no research has investigated the relationship between muscle damage, hormonal status, and perceived recovery scale (PRS). Therefore, the purpose of this study was to determine the effects of a high volume training session on PRS and to determine the relationship between levels of testosterone, cortisol and creatine kinase (CK) and PRS. Thirty-five trained subjects (21.3 ± 1.9 years) were recruited. All subjects participated in a high volume resistance training session consisting of 3 sets of full squats, bench press, deadlifts, pullups, dips, bent over rows, shoulder press, and barbell curls and extensions. Pre and post PRS scale measurements (0-10), soreness, creatine kinase (CK), cortisol, and testosterone were measured prior to and 48 hours following training. PRS declined from 8.6 ± 2.3 to 4.2 ± 1.85 (p < 0.05). Leg, chest, and arm soreness increased from pre to post exercise. Creatine kinase significantly increased from pre to post workout (189.4 ± 100.2 to 512 ± 222.7 U/L). Cortisol, testosterone, and free testosterone did not change. There was an inverse relationship between CK and PRS (r=0.58, p < 0.05). When muscle damage was low prior to training, cortisol, free and total testosterone were not correlated to PRS. However, when damage peaked at 48 hours post exercise, free, but not total testosterone, showed a low, direct relationship with PRS (r=0.2, p < 0.05). High volume resistance exercise lowers PRS scores. These changes are partly explained by a rise in serum indices of muscle damage. Moreover, free testosterone appears to have a positive relationship to PRS.