Carotenoids Extraction Optimization of Lutein-Based Banana Peel

Article · January 2015with 404 Reads
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Abstract
Carotenoids are among pigments with antioxidant properties and healthy effects on human body. Lutein is one of the most important carotenoids that is abundantly found in banana peel and belongs to xanthophyll's family. The present study aims to banana extract in order to use the applied properties of its carotenoids specially lutein. The banana peels were dried in vacuum oven and changed to powder, three solvents of ethanol 96%, methanol 99.5% and ethyl acetate 80% were used in 27 and 35 centigrade to extract its juice and study the amount of lutein. HPLC, UV-VIS spectrophotometer and FRAP test were respectively used to determine the amount of lutein and the antioxidant power of the extracts. The findings showed that considering the inhibition time of pure lutein with HPLC that took about 2.311 minutes, the amount of lutein gained by ethyl acetate solvent was more as compared to other extracts and its peak area was about 400.Similarly, through UV-VIS colorimetry and considering the amount of light absorbance of pure lutein, the amount of light absorbance in ethyl acetate solvent in 27°C was more as compared to other samples and its amount of absorbance was about 2.204.The antioxidant power of ethyl acetate in 27°C in FRAP method showed the higher amount.

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