Influence of Epilobium angustifolium extract on 5?-reductase type 2 and MAPK3 kinase gene expression in rats prostates

Abstract

The aim of this study was to investigate the influence of standardized Epilobium angustifolium
L. extract [100 mg/kg/day, p.o.] on the expression level of 5α-reductase type 2 (Srd5ar2) mRNA and Mapk3 mRNA a representative of non-genomic xenobiotics signaling pathway. It
was shown that plant extract from the E. angustifolium showed a slight tendency to reduce
prostate weight in hormonally induced animals (p>0.05) and in testosterone induced animals
receiving both, extract and finasteride (p<0.05). Finasteride in rats induced by testosterone
caused a smaller decrease in the level of mRNA 5α-steroid reductase 2 (SRd5ar2),
than in rats treated with the hormone and studied plant extracts. In general, an increase in
the amount of MAPK3 mRNAs in testosterone-induced groups of rats receiving tested plant
extract with or without finasteride was observed, while the expression of type 2 5α-steroid
reductase decreased (p<0.05). Further experimental studies should be performed in order
to understand the molecular basis of interactions, the efficacy and safety of tested plant
extracts.

Full text

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


        
   



  
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      
α
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



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  
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          
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 
        
       


   

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



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β


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
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

 


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α


   
 
 
           

  

    
    
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 

          

   













→



   
    º º º
 
    º º º
 
    º º º
 











α


Epilobium angustifolium


          


  




  




 



  
  
  
  
  
  
  

 
 
 
 
 








Figure 1.
Rat prostates weight changes [mg] in different groups (n=10): K1, K2 – control groups; E –
animals administered with E. angustifolium extract; T – testosterone administered animals;
TE – testosterone + E. angustifolium administered rats; TF – testosterone with finasteride
administered animals; TFE - testosterone with E. angustifolium and finasteride administered
animals. Data presented as mean ±SEM in each group. *p<0.05 as compared to control group
(one-way ANOVA test)



  
 

Epilobium angustifolium α

    
      α    
         





  


  





α

Figure 2.
Fold changes in mRNA expression level of 5α-steroid reductase type 2 (5ar2) in the ventral
lobe of rat prostates influenced with Epilobium angustifolium extract. K – control group; E –
animals administered with E. angustifolium extract; T – testosterone administered animals;
TE – testosterone + E. angustifolium administered rats; TF – testosterone with finasteride
administered animals; TFE - testosterone with E. angustifolium and finasteride administered
animals; Data were presented as mean ±SEM in each group. *p<0.05 as compared with th
control group (one-way ANOVA test)

α

 
  


 Epilobium angustifolium







        
 







Figure 3.
Fold changes in mRNA expression level of MAPK3 kinase in the ventral lobe of rat prostates
influenced by Epilobium angustifolium extract. K – control group; E – animals administered
with E. angustifolium extract; T – testosterone administered animals; TE – testosterone + E.
angustifolium administered rats; TF – testosterone with finasteride administered animals; TFE
– testosterone with E. angustifolium and finasteride administered animals. Data were
presented as mean ±SEM in each group. *p<0.05 as compared to control group (one-way
ANOVA test)

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 
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
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    

α




α

             

       α       
  

 
α

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




            


               
         α 
α
 
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  

     

 
  

 
 
 

 
              

  


  

 


               

 

 

                


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
 

 
γ 
    


  
     


                 
            

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             
   

 
α

 α

 
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β
             

    
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 

      

  

 
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 
   

 
 α
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             
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 α
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 

              
          


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