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Medicinal plants useful in fish diseases

Authors:
  • SELF-RELIANT POSITON and Formerly at Nanaji Deshmukh Veterinary Science University, Jabalpur, MP, India
  • College of fishery science, jabalpur

Abstract

In fish, the parasitic outbreak acts as an important limiting factor for aquaculture. The heavy infection of Trichodina parasites has caused gigantic financial losses. Diseases caused by Aeromonas hydrophila bacterium are some of the most widespread in freshwater fish culture. Nowadays, a large portion of the world population, especially in developing countries depends on the traditional system of medicine for a variety of diseases. The medicinal plants (herbal drugs) can be used not only against diseases but even more so, as growth promoters, stress resistance boosters and preventatives of infections. Additionally, phytomedicines provide a cheaper source for treatment and greater accuracy than chemotherapeutic agents without causing toxicity. The phytochemicals, e.g., tannins, alkaloids and flavonoids present in medicinal plants may have antimicrobial activity. The herbs can also act as immunostimulants, conferring the non-specific defense mechanisms of fish and elevating the specific immune response. In the present article, some herbs acting against fish diseases, especially parasites, bacteria and immuno-suppression have been discussed.
MEDICINAL PLANTS USEFUL IN FISH DISEASES
Govind Pandey1*, Madhuri Sharma2 and A. K. Mandloi3
1Officer-In-Charge, Rinder Pest (Animal Husbandry Deptt., Govt. of MP), Jabalpur Division, Jabalpur (M.P.), India.
2Department of Zoology and Biotechnology, Govt. Model Science College, Jabalpur (M.P.), India.
3Department of Fisheries, College of Veterinary Science & A.H., Jabalpur (M.P.), India.
Abstract
In fish, the parasitic outbreak acts as an important limiting factor for aquaculture. The heavy infection of Trichodina parasites
has caused gigantic financial losses. Diseases caused by Aeromonas hydrophila bacterium are some of the most widespread
in freshwater fish culture. Nowadays, a large portion of the world population, especially in developing countries depends on
the traditional system of medicine for a variety of diseases. The medicinal plants (herbal drugs) can be used not only against
diseases but even more so, as growth promoters, stress resistance boosters and preventatives of infections. Additionally,
phytomedicines provide a cheaper source for treatment and greater accuracy than chemotherapeutic agents without causing
toxicity. The phytochemicals, e.g., tannins, alkaloids and flavonoids present in medicinal plants may have antimicrobial
activity. The herbs can also act as immunostimulants, conferring the non-specific defense mechanisms of fish and elevating
the specific immune response. In the present article, some herbs acting against fish diseases, especially parasites, bacteria
and immuno-suppression have been discussed.
Key words : Fish pathogens, herbs, medicinal plants (herbal drugs), treatment of fish diseases.
Plant Archives Vol. 12 No. 1, 2012 pp. 1-4 ISSN 0972-5210
Introduction
Although herbal remedies have been with us for
human therapy for millennia, there has been relatively
little research on the medicinal plants to be used against
fish diseases. Herbal drugs can be used not only as
remedies but even more so, as growth promoters, stress
resistance boosters and preventatives of infections.
Hence, herbal drugs in disease management are gaining
success, because they are cost effective, eco-friendly
and have minimal side effects. A large portion of the
world population, especially in developing countries
depends on the traditional system of medicine for a variety
of diseases. Several hundred genera are used medicinally
and plants are vital sources for potent and powerful drugs.
Plants are rich in a wide variety of secondary metabolites
of phytochemical constituents such as tannins, alkaloids
and flavonoids, which act against different diseases
(Pandey and Madhuri, 2010; Ravikumar et al., 2010).
Unfortunately, the parasitic outbreak acts as an
important limiting factor for aquaculture businesses.
Pinkate et al. (2003) reported that every tilapia fish
(Oreochromis niloticus) raised by farmers in Chiang
Mai, Thailand has a Trichodina parasite infection.
Chitmanat et al. (2005) pointed out that the heavy
infection of Trichodina sp. in small fish has caused
gigantic financial losses. Infected fish are lethargic,
generate excessive mucus and become off-feed
eventually, resulting into considerable deaths. There is
now a fast growing interest in screening antiparasitic
substances from plants to replace chemical and antibiotic
alternatives.
Diseases caused by A. hydrophila bacterium are
some of the most widespread in freshwater fish culture.
Septicaemia caused by motile aeromonads is a ubiquitous
problem that affects fishes found in warm, cool and cold
fresh water around the world. A. hydrophila has been
associated with diseases in fishes like carp, eels, milkfish,
channel catfish, tilapia and ayu. This microorganism can
also be an opportunist in stress-related diseases in
salmonids. Antibiotics are frequently used to control
disease caused by this bacterium, but there is an
increasing risk of developing antibiotic resistant (Yin et
al., 2008). A. hydrophila is also responsible for skin
infections, septicemia and gastroenteritis in human,
besides the fish (Castro et al., 2008). The continuous
use of antimicrobial agents in aquaculture has resulted in
more resistant bacterial strains. Since ancient times,
medicinal plants have been used for the treatment of
Review Article
*Author for correspondence : drgovindpandey@rediffmail.com
common infectious diseases and treatments with plants
having antibacterial activity are a potentially beneficial
alternative in aquaculture. Additionally, phytomedicines
provide a cheaper source for treatment and greater
accuracy than chemotherapeutic agents in fish (Abdul
Kader Mydeen and Haniffa, 2011; Turker et al., 2009).
A. hydrophila, the most common bacterial pathogen in
freshwater fish, has been recognized to be the aetiological
agent of several distinct pathological conditions, including
tail rot, motile Aeromonas septicemia (MAS) and
epizootic ulcerative syndrome (EUS) as a primary
pathogen. EUS is a globally distributed disease and has
become an epidemic affecting a wide variety of wild and
cultured fish species in South-east Asia, including India.
Ability of herbs to inhibit activity of bacteria having
potential interest as fish pathogens has been documented.
Heavy antibiotics in aquaculture should be reduced and
replaced with alternative processes in fish diseases to
avoid the emergence of antibiotic resistance in pathogenic
and environmental bacteria (Abdul Kader Mydeen and
Haniffa, 2011). The phytochemicals, e.g., tannins, alkaloids
and flavonoids present in the medicinal plants have been
found in vitro to have antimicrobial properties. Many of
the spices and herbs have been valued for their
antimicrobial effects and medicinal powers in addition to
their flavour and fragrance qualities. Recently, research
has been initiated to evaluate the feasibility of using herbal
medicines in fish disease management. Moreover, the
bacterial infections are considered the major cause of
mortality in aquaculture and in the recent years, medicinal
plants and their products play significant role in fish culture
(Ravikumar et al., 2010).
Herbs can act as immunostimulants, conferring early
activation to the non-specific defense mechanisms of fish
and elevating the specific immune response. Chinese herbs
have been used an medicine to treat different fish diseases
in China for many years. Herbs have also been used in
other countries for control of shrimp and fish diseases,
and successful results have been reported in Mexico,
India, Thailand and Japan (Yin et al., 2008). Traditional
herbal medicines seem to be the potential
immunostimulator. Thus, the use of medicinal plants is an
alternative to antibiotics in fish health management. The
herbs are not only safe for consumers, but they also have
a significant role in aquaculture. Many studies have proved
that herbal additives enhanced the growth of fishes and
protected from diseases. The non-specific immune system
of fish is considered to be the first line of defense against
invading pathogens (Ahilan et al., 2010). Many plant-
compounds have been found to have non-specific immuno-
stimulating effects in humans and animals (Pandey and
2Govind Pandey et al.
Madhuri, 2010; Kolkovski and Kolkovski, 2011) of which
more than a dozen have been evaluated in fish and
shrimp. Several plant products seemed to be potent
antiviral agents against fish and shrimp viruses (Kolkovski
and Kolkovski, 2011).
Therefore, the herbal plants may be used as potential
and promising source of pharmaceutical agents against
fish pathogens in organic aquaculture (Abdul Kader
Mydeen and Haniffa, 2011; Ravikumar et al., 2010). In
view of the above context, the usefulness of some
medicinal plants (herbal drugs) against fish diseases has
been elucidated in the present article.
Some on medicinal plants acting against fish
diseases
To preserve and protect the environment as well as
human health as a best alternative, different parts of
Azadirachta indica (Neem) tree have been studied by
Chitmanat et al. (2005). Neem leaves containing nimbin,
azadirachtin and meliantroil have been reported to possess
a variety of properties, including insecticidal and antiviral
from ancient times. Indian almond (Terminalia catappa)
and garlic (Allium sativum) have been said as an
alternative to chemicals to treat fish ectoparasites,
Trichodina sp. infections in tilapia (O. niloticus)
fingerlings. Both Indian almond and garlic had low acute
toxicity to tilapia fingerlings, treating the trichodiniasis
caused by Trichodina. The authors further cited that the
Indian almond, commonly used as herb in Taiwan,
prevents the fish diseases. It is claimed to be a wound
healing substance for Siamese fighting fish hurt after
matches in Thailand as well. The immunostimulant effects
of the dietary intake of 3 plants (viz., Viscum album,
Urtica dioica and Zingiber officinale)-extracts on
rainbow trout (Oncorhynchus mykiss) have also been
narrated by the authors. Christybapita et al. (2007)
observed the immunostimulatory effect of aqueous extract
(AqE) of Eclipta alba (Bhangra) leaf (oral administration
as feed supplement) in tilapia fish, Oreochromis
mossambicus. It was noted that the E. alba extract
enhances non-specific immune responses and disease
resistance of O. mossambicus against A. hydrophila
infection. According to Winkaler et al. (2007), A. indica
extract can be used successfully in aquaculture to control
fish predators.
Castro et al. (2008) screened the methanolic extracts
of 46 Brazilian plants and found only 31 to have the
antibacterial activities against fish pathogenic bacteria,
viz., Streptococcus agalactiae, Flavobacterium
columnare and A. hydrophila. Yin et al. (2008) added
the extracts of 2 Chinese herbs (viz., Lonicera japonica
and Ganoderma lucidum) in diets of tilapia fish (O.
niloticus) and found that these herbs act as
immunostimulants and appear to improve the immune
status and disease resistance. Both herbs when used alone
or in combination increased the survival of fish after
challenge with A. hydrophila. On the basis of several
studies, Yin et al. (2008) reported that oral administration
of ginger (Z. officinale) extract increases the phagocytic
capability of cells in rainbow trout (fish), while the extracts
of 4 Chinese herbs (Rheum officinale, Andrographis
paniculata, Isatis indigotica and Lonicera japonica)
increased the phagocytosis of white blood cells of carp.
Turker et al. (2009) reported that the alcoholic and
aqueous extracts of Nuphar lutea, Nymphaea alba,
Stachys annua, Genista lydia, Vinca minor, Fragaria
vesca, Filipendula ulmaria and Helichrysum plicatum
herbs of Bold (Turkey) have antibacterial activity against
A. hydrophila, Yersinia ruckeri, Lactococcus
garvieae, Str. agalactae and Enterococcus faecalis
bacteria isolated from fish. This observation provides the
aquaculturists with a promising management tool for
control or treatment of fish diseases. Nya and Austin
(2009) observed the control of A. hydrophila infection
after feeding with A. sativum (0.5 and 1 g/100 g of feed
for 14 days) to rainbow trout (fish), O. mykiss (Walbaum).
Ahilan et al. (2010) observed that the addition of
Phyllanthus niruri and Aloe vera (Aloe) as herbal
additives can positively enhance the growth performance
of goldfish, Carassius auratus as well as its resistance
to A. hydrophila infections. The authors further reported
that the herbal additives in diets often provide cooperative
action to various physiological functions. The synergistic
effect of herbs has been reported in other fishes, including
Japanese flounder and Clarias gariepinus. The growth
increase in Labeo rohita fish fed with herbal
supplemented diet was due to improved food utilization
and high protein synthesis. The benefit of herbal growth
promoters as an additive in the carp feed has also been
found. Furthermore, the medicinal plants, viz., ginger, nettle
and mistletoe as an adjuvant therapy in rainbow trout
through feed enhanced phagocytosis and cellular and
humoral defense mechanisms against pathogens. The
traditional Chinese medicines in yellow croaker elevated
the non specific defense mechanism and increased the
disease resistance of fish against bacterial pathogens.
The disease resistant of Catla catla fish was produced
through immersion treatment of 3 herbs, viz., A. sativum,
A. indica and Curcuma longa (Haldi rhizome, turmeric)
in spawn. A. vera has been found to a disease suppressing
agent and showed antibacterial effect in juvenile rock
fish. Harikrishnan et al. (2010) reported that mixed herbal
Medicinal Plants Useful in Fish Diseases 3
extracts supplementation diets restored the altered
haematological parameters and triggered the innate
immune system of goldfish (C. auratus) against A.
hydrophila infection.
Ravikumar et al. (2010) observed that the chloroform
extract of Datura metal plant has wide range of
antimicrobial activity against many fish pathogens. D.
metel which collected from the Kanyakumari coast can
be used as a putative antimicrobial drug in the aquaculture
maintenance. The chloroform extract of D. metel can be
effectively used as a potential antimicrobial agent to
overcome the problem of mass mortality of ornamental
fish in aquarium so as to enable to enhance the market
revenue throughout the world. These authors also told
the antimicrobial activity of 5 Chinese herb extracts
against 13 bacterial and 2 viral fish pathogens. Sharma
et al. (2010) observed the stimulatory effect of dietary
doses of Withania somnifera (Ashwagandha) root on
immunity and disease resistance against A. hydrophila
infection in Indian major carp, L. rohita fingerlings. Abdul
Kader Mydeen and Haniffa (2011) cited that A. indica
leaf AqE could effectively control the A. hydrophila
infection in common carp (a fresh-water fish usually bred
in ponds), Cyprinus carpio. Further, Enterobacter sp.
and Escherichia coli bacteria, isolated from marine fish
(Amphiprion sebae) showed 15 mm zone of inhibition
against neem extract. The antimicrobial activity of AqE
of 3 medicinal plants, viz., A indica (leaf), Solanum
torvum (Sundakai fruit coat) and C. longa (rhizome)
against the in vitro growth of A. hydrophila, isolated
from infected fresh-water fish, Channa striatus was
noticed by Abdul Kader Mydeen and Haniffa (2011).
Kolkovski and Kolkovski (2011) reported that some herbal
extracts are very effective against gills and skin flukes
like Benedenia seriolae. Nargis et al. (2011) seen the
immunostimulant effects of the dietary intake of A.
sativum and Vitex negundo extracts on fingerlings of L.
rohita fish. Ravikumar et al. (2011) studied that among
15 coastal medicinal plants/ parts of plants, A. indica,
Cinnamomum verum and Eupatorium odoratum
exhibited excellent antibacterial activity against 10
bacterial pathogens from diseased ornamental fishes.
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... However, due to increasing environmental and food safety concerns associated with these harmful chemicals; and increasing consumer awareness, the modern world is now searching for alternate effective safe organic alternative solutions to achieve envisaged production targets. In this context, a wide range of medicinal herbs with growth-promoting and immuno-stimulating capabilities, offer a cost-effective eco-friendly alternative for production enhancement in livestock and fisheries sector [74], [20], [8], [61]. The medicinal herbs contain various bioactive substances, like alkaloids, flavonoids, pigments, phenols, saponins, steroids and essential oils, well known for multiple health benefits since ages [8], [61]. ...
... In this context, a wide range of medicinal herbs with growth-promoting and immuno-stimulating capabilities, offer a cost-effective eco-friendly alternative for production enhancement in livestock and fisheries sector [74], [20], [8], [61]. The medicinal herbs contain various bioactive substances, like alkaloids, flavonoids, pigments, phenols, saponins, steroids and essential oils, well known for multiple health benefits since ages [8], [61]. A wide range of medicinal plants (garlic, ashwagandha, shatavari, amla, Aloe, neem, ginger, moringa etc.) have been successfully tested to enhance growth, immunity and reproductive performance in many fish [23], [1], [51], [13], [75], [76], [45], [44], [57], [30] and shell fish [11], [34], [66] species. ...
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... which contain nimbin, azadirachtin, and meliantroil, have a range of bioactivities, including insecticidal and antiviral capabilities (Pandey et al., 2012). Additionally, it has been observed that neem increases tilapia survival rates, growth, and feed utilization characteristics while not influencing hematological parameters (Gabriel, 2019). ...
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... Many herbs and herbal products are used in aquaculture techniques to treat ailments, increase growth, relieve stress, stimulate hunger, enhance immunity, and prevent infections in order to produce healthy fish (1)(2)(3)(4)(5). Herbal plant sources are being examined as a feasible option to be used in fish feed without sacrificing the nutritional content (6,7). ...
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Balık talebi, mevcut üretim miktarlarının kat kat üzerinde olan, değerli ve yeri doldurulamaz bir gıda ürünüdür. Tarımın en karlı dalı hiç şüphesiz ki balık yetiştiriciliğidir. Balıkçılığın gelişmesi için önemli bir şart balıkların hastalıklardan korunmasıdır. Balıklar da diğer hayvanlar gibi hastalıklara karşı hassastır. Çeşitli etiyolojiler (paraziter, bakteriyel, viral,mikotik, enfeksiyöz olmayan) hem balık üretim tesislerinde ve hem de doğal su kaynaklarında ortaya çıkmakta ve balık yetiştiriciliğine önemli zararlar vermektedir. Balıkların büyüme hızında ve üreme performansında önemli azalmalara, çeşitli deformasyon ve anomalilerin gelişmesine, balık ürünlerinin kalitesinde bozulmalara ve sonuçta balıkların toplu olarak ölümlerine neden olarak işletmelerin ekonomik olarak zarar etmesine sebep olurlar. Balıkları hastalıklardan korunmada temel amaç, olası hastalıkların öngörülmesi, önleme araçları ve yöntemleri ile hastalık ortaya çıkması durumunda tedavisinin gerçekleştirilmesidir. Balık yetiştiriciliğinde özellikle enfeksiyöz hastalıklar kısa vadede bile su ürünleri ticaretini olumsuz etkilemektedir. Özellikle son yıllarda artan balık üretimi balık sağlığı açısından sorunları da beraberinde getirmiştir. Bu yoğun balık üretimi sonrasında ortaya çıkan hastalıkların tedavisinde kullanılan antibiyotiklere karşı balıkların gösterdiği direnç, yeni enfeksiyon etkenlerinin kuluçkahanelerde ve yetiştiricilik tesislerinde ortaya çıkması ve bazı hastalık etkenlerine karşı tedavinin etkisiz kalması gibi bir çok probleminde oluşmasına olanak sağlamıştır. Sulardaki epizootik refahı sağlamak ve yüksek kaliteli balık ürünleri elde etmek için uzmanların yalnızca balık yetiştiriciliğinin biyolojisi ve biyoteknolojisi alanında bilgiye sahip olmaları yeterli değildir. Bu nedenle balıkçılıkta özellikle balık sağlığının sürdürülebilmesi ve işletmelerin karlılığının artırılması için hastalıklar ve ve onların patolojik özellikleri ile ilgili olarak da derin teorik ve pratik bilgiye sahip olmaları gerekir.
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The infestation of freshwater fish lice belonging to the genus Argulus occur repeatedly in freshwater fish farms due to poor management practices. Preventative methods and regular monitoring often fails to control the outbreak, and in tum chemical interventions become necessary. In the present study, the outbreak of freshwater fish lice Argulus in Rohu (Labeo rohita) has been reported at a farm in Kalyani, West Bengal. A framework was developed for sustainable eco-friendly and cost effective treatment of Argulus using salt at 10 g/L for 3 minute, followed by formalin at 40 L/L for 3 minute, potassium permanganate at 0.5 g/L for 3 minute and turmeric at 5 g/L for 3 minute, respectively in-vivo. Mean intensity, abundance and prevalence oftheArgulus sp. was assessed as the most suitable descriptors to determine the impact of the treatment. The parasite was grouped abundant (85%) in Labeo rohita prior to the treatment, whereas, post-treatment, the parasites became rare (5%) in the same fish. Significant reduction in the mean intensity and abundance of Argulus sp. was also recorded post-treatment, which implied the efficiency of the treatment process in eradicating Argulus. Combined with the multiple bath treatment, mechanical control through vigorous shaking of the affected fish was also found effective and hence may be recommended to the farmers as the suitable control strategy of the freshwater fish lice subjected to further validation in different field condition.
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Fish frequently come into contact with water-borne pathogens. Fish are susceptible to several illnesses caused by macro and microorganisms. As a result of ectoparasite Trichodina's widespread early infestation, significant financial losses have occurred. Currently, it appears that formalin is worthless for treating ectoparasitic diseases in freshwater fish. Furthermore, formalin may leave hazardous residues in the fish flesh and the environment that are ultimately damaging to consumers. But this problem can also be resolved by using medicinal plants. The therapeutic herbs can work as immunostimulants, providing fish with activating non-specific defensive mechanisms and enhancing their specialized immune response. Indian almonds and garlic have been used to cure Trichodina sp. for an ectoparasite of tilapia fish.
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Immunostimulants in feed additives have a crucial function in maintaining fish wellness and fostering systemic development by giving resistance to diseases that harm cultured fish. Innate immunity is essential for fish larvae to survive. Afterward, innate immunity is helpful in modulating acquired immune system response and regulation via numerous receptor proteins. On fish and shrimp, glucan, chitin, lactoferrin, and levamisole have been shown to have immunostimulatory effects. There have also been claims that nutritional components such as vitamins and hormones can stimulate the immune system. These immunostimulants primarily improve phagocytic cell activity and heighten their bactericidal actions. Enhanced defence against infectious disease is linked to the activation of various immune processes. Different pathways for the initiation of protective immune responses are activated as a result of different pathogen recognition receptors‘ differential identification of invading pathogens. Consequently, there is a growing desire to combat the debilitating consequences of illnesses rather than using harmful drugs or chemicals. Numerous immunostimulants are used to boost immunological reaction and protein expression levels via different signalling pathways, exploring potential alternatives to the use of toxic agents in the management of fish illnesses. This chapter's goal is to provide an overview and assessment of the current study of different immunostimulants and the immunological responses they induce in fish. Finally, immunostimulants help lessen disease-related losses in aquaculture. They might not be effective against all ailments, though. When using immunostimulants, it is important to consider the schedule, dosage, route of administration, and physiological status of fish.
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Cancer is the second leading cause of human deaths in the world and claims over 6 millions. In fact, at least 35% of all cancers worldwide are caused by incorrect diet. Malnutrition may cause several diseases, including cancer. Plants have original place in the treatment of cancer with no ill effect. It is distinct that medicinal plants, including fruits and vegetables have versatile immunomodulatory and anticancer activities. Consumption of large amounts of fruits and vegetables can prevent the development of cancer. Many doctors recommend that people wishing to reduce risk of cancer eat several pieces of fruits and several portions of vegetables every day. With this back ground, an attempt has been made to review on certain fruits and vegetables useful against various types of cancer.
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Alcoholic and aqueous extracts from 22 species of herbs from Bolu (Turkey) were screened for antibacterial activity against Aeromonas hydrophila, Yersinia ruckeri, Lactococcus garvieae, Streptococcus agalactiae and Enterococcus faecalis. Extracts with various solvent of Nuphar lutea, Nymphaea alba, Stachys annua, Genista lydia, Vinca minor, Fragaria vesca, Filipendula ulmaria, Helichrysum plicatum showed the highest inhibitory activity. The ethanolic extract of V. minor and the alcoholic and aqueous extract of N. lutea displayed a broad antibacterial spectrum against the target organisms. The possible usage of herbs as an alternative to synthetic antibiotics is discussed.
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The aim of this work was to evaluate the antibacterial activity of Brazilian plants extracts against fish pathogenic bacteria. Forty six methanolic extracts were screened to identify their antibacterial properties against Streptococcus agalactiae, Flavobacterium columnare and Aeromonas hydrophila. Thirty one extracts showed antibacterial activity.
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This study was conducted to investigate the bioactive potential of coastal medicinal plants to develop alternative drug development for the treatment of diseases in aquaculture. Fifteen coastal medicinal plants / parts were investigated to evaluate the antibacterial activity against bacterial fish pathogens. Among the plants, Cinnamomum verum and Eupatorium odoratum, Azardirachta indica exhibited excellent antibacterial activity against 10 bacterial pathogens from diseased ornamental fishes under captivity.
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Immunostimulant effects of the dietary intake of some medicinal plants extracts on fingerlings of Labeo rohita were investigated. For this purpose, fingerlings were fed with diets containing aqueous extracts of Nishinda (Vitex negundo) and garlic (Allium sativum) pest. For immunostimulatory food additives caused a good result compared to the control group. After 90 days experiment SGR% (Specific growth rate) is in aquarium A, B and control were 0.92, 0.89 and 0.61 respectfully. The mortality rate % in A, B and control were 44, 40 and 76 respectively.
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This study evaluated the antimicrobial potency of aqueous extract of three common medicinal herbs, Azadirachta indica (Neem leaves), Solanum torvum (Sundakai fruit coat) and Curcuma longa (Turmeric,rhizome) against the in vitro growth of pathogenic bacterium Aeromonas hydrophila isolated from infected fresh- water fish, Channa striatus was assessed by disc diffusion assay. Determination of minimum inhibitory concentration (MIC) for each aqueous herbal extract was done on Muller Hinton agar swabbed with 0.5 ml of overnight culture of A. hydrophila (10 6 cfu/ml). After incubation period, zones of inhibition around herbal extracts incorporated discs were measured. The strongest antibacterial activities among all plant species were obtained by the aqueous extract of A. indica with inhibition zone of 18 mm against A. hydrophila. S. torvum demonstrated moderate (11 mm) and C. longa marked weak (8 mm) inhibiting activity against A. hydrophila.
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Goldfish, Carassius auratus (47 + or - 3 g, n = 300) were inoculated intramuscularly (50 microl) with Aeromonas hydrophila (1.8 x 10(6) cells ml(-1)). On the 6th day of post-infection the fishes were divided into i) control, without infection fed with normal diet (C), ii) infected fish, fed with normal diet (IU), and infected fishes treated with different doses of iii) 100 mg kg(-1), iv) 200 mg kg(-1), iv) 400 mg kg(-1) and vi) 800 mg kg(-1) mixed herbal extracts supplementation diets. Hematological and immunological parameters were determined on week 1, 2 and 4. In infected goldfish were fed diets containing 100 and 200 mg kg(-1) of mixed herbal extracts supplementation feeds, the white blood cell (WBC) levels significantly increased (P < 0.05) throughout the experimental trial compared to the control. During the experimental period, the red blood cell (RBC) and haemoglobin (Hb) level in goldfish significantly decreased (P < 0.05) when fish fed with 100 and 200 mg kg(-1) of mixed herbal extracts supplementation feeds while it was restored near control when infected fish fed with 400 or 800 mg kg(-1) of herbal extracts supplementation feeds. On the other hand, the haematocrit (Ht) values decline significantly (P < 0.05) in 100, 200 and 400 mg kg(-1) of mixed herbal supplementation feeding groups on weeks 2 and 4 when compared to control group. The mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) values almost significantly differ from the control values. The infected goldfish and treated with 100 or 200 mg kg(-1) of herbal supplementation feeds exhibited significantly decline (P < 0.05) in total protein (TP), glucose (GLU) and cholesterol (CHO) levels on week 1-4 whereas it was restored when infected fish fed with 400 or 800 mg kg(-1) of herbal supplementation feeds on week 4. In comparison to untreated control goldfish, the respiratory burst activity and phagocytic activity of blood cells was significantly enhanced in infected fish feeding with 200, 400 and 800 mg kg(-1) of herbal supplementation feeds compared to the control. On the other hand, infected fish fed with all the doses of mixed herbal supplementation feeds, the lysozyme activity was significantly enhanced throughout the experimental period. This study shows that the infected goldfish treated with 400 and 800 mg kg(-1) of herbal supplementation feeds preceding the challenge with live A. hydrophila had 30% and 25% mortality. However, 100 and 200 mg kg(-1) of herbal supplementation feeds treated groups were found the percentage mortality 50% and 45%, respectively. Our results indicate that 400 or 800 mg kg(-1) of mixed herbal supplementation feeds were restored the altered hematological parameters and triggering the innate immune system of goldfish against A. hydrophila.