Antioxidant and Antidiarrhoeal Potentiality of Diospyros blancoi
Phytochemical analysis of the MeOH extract of leaves of Diospyros blancoi (Ebenaccae) indicated the presence of Reducing Sugar, Gum, Tannin, Alkaloid and Flavonoid types of compounds. The pharmacological interest of these compounds prompted us to check its antidiarrhoeal and anti-oxidant activities. Antioxidant potential was evaluated by using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay. The extract showed significant (p<0.001) antioxidant activity in a dose-dependent manner with the IC50 value of 15.06 +/- 0.49 mu g mL(-1), compared to the IC50 value of the reference standard ascorbic acid which was 8.11 +/- 0.2 1 mu g mL(-1) suggested its potent anti-oxidant activity. The anti-diarrhoeal activity was investigated on the castor oil-induced mice models. The doses displayed significant reduction in faecal output of about 24.49 and 44.89%, respectively at 250 and 500 mg kg(-1), compared to the reference standard loperamide which was 59.18% mu g mL(-1). The obtained results provide a support for the use of this plant in traditional medicine as an antidiarrhoeal remedy.