The potential of an express enzymological indicator – phosphatase activity index (PAI) – for assessment of different types of pollution and self-purification potential in running waters was evaluated for three river subcatchments, representative for different ecological situations and impacts. According to the values of correlation coefficients, a significant positive correlation existed among
... [Show full abstract] PAI and total microbial count, organic loading and phosphate concentrations. The enzyme activity is useful tool for early identification of risks from point discharge of different wastewaters (treated or non-treated). The role of PAI as an indicator is significant at case of disinfection after treatment of wastewaters in treatment plant – the aquatic microbial community at the discharge point is suppressed but not at a functional level. The results allow classical microbiological and chemical parameters (state variables) to be related directly to the dynamics of the transformation processes by functional variable – PAI.