The overexpression of a gene of interest by third generation lentiviral particle generation systems is a critical process in molecular biology, cell biology and gene therapy research. While many lentiviral protocol production methods have been discussed in literature, this protocol takes into account previously established optimisations with the aim to minimise user handling time, cost, and
... [Show full abstract] maximise practical yield. This protocol allows for at least 6 days of consecutive viral particle collection without compromising HEK293T cell culture or viral production efficiency, and can be easily and cost effectively reproduced in basic cell culture laboratories.
