Article

MethMarker: User-friendly design and optimization of gene-specific DNA methylation assays

Max-Planck-Institut für Informatik, Saarbrücken, Germany.
Genome biology (Impact Factor: 10.81). 10/2009; 10(10):R105. DOI: 10.1186/gb-2009-10-10-r105
Source: PubMed

ABSTRACT

DNA methylation is a key mechanism of epigenetic regulation that is frequently altered in diseases such as cancer. To confirm the biological or clinical relevance of such changes, gene-specific DNA methylation changes need to be validated in multiple samples. We have developed the MethMarker http://methmarker.mpi-inf.mpg.de/ software to help design robust and cost-efficient DNA methylation assays for six widely used methods. Furthermore, MethMarker implements a bioinformatic workflow for transforming disease-specific differentially methylated genomic regions into robust clinical biomarkers.

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