Differential activation of limbic circuitry associated with chronic ethanol withdrawal in DBA/2J and C57BL/6J mice

Department of Behavioral Neuroscience and Portland Alcohol Research Center, Oregon Health & Science University, Portland, OR 97239-03098, USA.
Alcohol (Fayetteville, N.Y.) (Impact Factor: 2.01). 09/2009; 43(6):411-20. DOI: 10.1016/j.alcohol.2009.05.003
Source: PubMed


Although no animal model exactly duplicates clinically defined alcoholism, models for specific factors, such as the withdrawal syndrome, are useful for identifying potential neural determinants of liability in humans. The well-documented difference in withdrawal severity following chronic ethanol exposure, between the DBA/2J and C57BL/6J mouse strains, provides an excellent starting point for dissecting the neural circuitry affecting predisposition to physical dependence on ethanol. To induce physical dependence, we used a paradigm in which mice were continuously exposed to ethanol vapor for 72h. Ethanol-exposed and air-exposed (control) mice received daily injections of pyrazole hydrochloride, an alcohol dehydrogenase inhibitor, to stabilize blood ethanol levels. Ethanol-dependent and air-exposed mice were killed 7h after removal from the inhalation chambers. This time point corresponds to the time of peak ethanol withdrawal severity. The brains were processed to assess neural activation associated with ethanol withdrawal indexed by c-Fos immunostaining. Ethanol-withdrawn DBA/2J mice showed significantly (P<.05) greater neural activation than ethanol-withdrawn C57BL/6J mice in the dentate gyrus, hippocampus CA3, lateral septum, basolateral and central nuclei of the amygdala, and prelimbic cortex. Taken together with results using an acute model, our data suggest that progression from acute ethanol withdrawal to the more severe withdrawal associated with physical dependence following chronic ethanol exposure involves recruitment of neurons in the hippocampal formation, amygdala, and prelimbic cortex. To our knowledge, these are the first studies to use c-Fos to identify the brain regions and neurocircuitry that distinguish between chronic and acute ethanol withdrawal severity using informative animal models.

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Available from: Laura B Kozell
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    • "Separate studies gave bilateral microinjections of ALLO or FIN into the substantia nigra reticulata (SNR) or ventral tegmental area (VTA). The SNR is important in the propagation of convulsive activity (e.g., Gale, 1988; Velı´sˇkovaánd Moshe´, 2006), and both the SNR and VTA exhibit an activation in c-fos expression during acute (Kozell et al., 2005) and chronic (Chen et al., 2009; Olive et al., 2001) EtOH withdrawal. We predicted that WSP-1 male mice would exhibit behavioral tolerance to the anticonvulsant effect of intra-SNR and intra- VTA ALLO during EtOH withdrawal and that intra-SNR and intra-VTA FIN would enhance EtOH withdrawal severity. "
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    • "The LS, in particular, has been shown to regulate ethanol consumption [8], [55]. Moreover, the LS showed greater neural activity following alcohol withdrawal [56]. This particular study used the parent strains of the BXD RI line (B6 and D2), which are known to differ in sensitivity to alcohol and to its withdrawal [57]. "
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