Generation of Induced Pluripotent Stem Cells from Human Cord Blood Using OCT4 and SOX2

Center of Regenerative Medicine in Barcelona, Dr. Aiguader 88, Barcelona, Spain.
Cell stem cell (Impact Factor: 22.27). 10/2009; 5(4):353-7. DOI: 10.1016/j.stem.2009.09.008
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    • "Behav. (2015), cells (Li et al., 2009; Zhao et al., 2010), cells present in urine (Zhou et al., 2012) and various other cell types (Aoki et al., 2010; Bar-Nur et al., 2011; Eminli et al., 2009; Giorgetti et al., 2009; Haase et al., 2009; J.B. Kim et al., 2009; Liu et al., 2010; Nakagawa et al., 2008; Sugii et al., 2011; Yu et al., 2007). Although a well-established cell type in many fields of research, due to their ease of handling and the costeffectiveness , there are disadvantages to the use of fibroblasts as a starting cell type for producing hiPSCs. "
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    • "After choosing the target donor somatic cell type, it is necessary to select a cocktail of reprograming factors that usually are the four above mentioned. In few situations less than four factors are needed, such as in the case of cord blood CD133+ cells and keratinocytes (117). Through the reprograming process, the chromatin remodeling plays an essential role in the procurement of pluripotency. "
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    ABSTRACT: Natural killer (NK) cells play an essential role in the fight against tumor development. Over the last years, the progress made in the NK-cell biology field and in deciphering how NK-cell function is regulated, is driving efforts to utilize NK-cell-based immunotherapy as a promising approach for the treatment of malignant diseases. Therapies involving NK cells may be accomplished by activating and expanding endogenous NK cells by means of cytokine treatment or by transferring exogenous cells by adoptive cell therapy and/or by hematopoietic stem cell transplantation. NK cells that are suitable for adoptive cell therapy can be derived from different sources, including ex vivo expansion of autologous NK cells, unstimulated or expanded allogeneic NK cells from peripheral blood, derived from CD34+ hematopoietic progenitors from peripheral blood and umbilical cord blood, and NK-cell lines. Besides, genetically modified NK cells expressing chimeric antigen receptors or cytokines genes may also have a relevant future as therapeutic tools. Recently, it has been described the derivation of large numbers of functional and mature NK cells from pluripotent stem cells, both embryonic stem cells and induced pluripotent stem cells, which adds another tool to the expanding NK-cell-based cancer immunotherapy arsenal.
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    • "Thus, a faster protocol such as conversion of human patient-specific pluripotent stem cells and fibroblasts directly to retinal photoreceptors by forced expression of photoreceptor-expressed transcription factors might be more suitable to the use of these nanoparticles. Cord blood cells and keratinocytes have been shown to be more amenable for reprogramming, in terms of speed and efficiency, than skin fibroblasts.39,40 Cord blood cells have a high population of progenitor stem cells, and the keratinocyte is an epithelial cell type similar to the embryonic stem cell, whereas dermal fibroblasts (especially adult) are differentiated mesenchymal cells that are close to senescence,41,42 can possess somatic mutations,43,44 and have to undergo mesenchymal to epithelial transition during reprogramming. "
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