Characterization and Virus-Induced Expression Profiles of the Porcine Interferon-ω Multigene Family

State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, People's Republic of China.
Journal of interferon & cytokine research: the official journal of the International Society for Interferon and Cytokine Research (Impact Factor: 2). 10/2009; 29(10):687-93. DOI: 10.1089/jir.2008.0060
Source: PubMed


Interferon-omega is a member of the type I interferon family. In this work, 8 functional porcine interferon-omega genes and 4 pseudogenes present on porcine chromosome 1 were identified in the porcine genome database by BLAST scanning. Their genetic and genomic characteristics were investigated using bioinformatics tools. Then the PoIFN-omega functional subtype genes were isolated and expressed in BHK-21 cells. The PoIFN-omega subtypes possessed about 10(4) to 10(5) units of antiviral activity per milliliter. PoIFN-omega 7 had the highest antiviral activity, about 20 times that of PoIFN-omega 4, which had the lowest antiviral activity. Differential expression of the subtypes was detected in PK15 cells and porcine peripheral blood mononuclear cells (PBMCs) in response to pseudorabies virus and poly(I).poly(C). Expression of PoIFN-omega 2/-omega 6 was up-regulated to the greatest extent by virus infection.

Download full-text


Available from: Mingqiu Liu, Aug 30, 2014
  • Source

    Full-text · Article · Mar 2009 · Veterinary Immunology and Immunopathology
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Type I interferons (IFNs) are central to innate and adaptive immunity, and many have unique developmental and physiological functions. However, in most species, only two subtypes, IFN-alpha and IFN-beta, have been well studied. Because of the increasing importance of zoonotic viral diseases and the use of pigs to address human research questions, it is important to know the complete repertoire and activity of porcine type I IFNs. Here we show that porcine type I IFNs comprise at least 39 functional genes distributed along draft genomic sequences of chromosomes 1 and 10. These functional IFN genes are classified into 17 IFN-alpha subtypes, 11 IFN-delta subtypes, 7 IFN-omega subtypes, and single-subtype subclasses of IFN-alphaomega, IFN-beta, IFN-epsilon, and IFN-kappa. We found that porcine type I IFNs have diverse expression profiles and antiviral activities against porcine reproductive and respiratory syndrome virus (PRRSV) and vesicular stomatitis virus (VSV), with activity ranging from 0 to >10(5) Whereas most IFN-alpha subtypes retained the greatest antiviral activity against both PRRSV and VSV in porcine and MARC-145 cells, some IFN-delta and IFN-omega subtypes, IFN-beta, and IFN-alphaomega differed in their antiviral activity based on target cells and viruses. Several IFNs, including IFN-alpha7/11, IFN-delta2/7, and IFN-omega4, exhibited minimal or no antiviral activity in the tested target cell-virus systems. Thus comparative studies showed that antiviral activity of porcine type I IFNs is virus- and cell-dependent, and IFN-alphas are positively correlated with induction of MxA, an IFN-stimulated gene. Collectively, these data provide fundamental genomic information for porcine type I IFNs, information that is necessary for understanding porcine physiological and antiviral responses.
    Preview · Article · Jul 2010 · Physiological Genomics
  • [Show abstract] [Hide abstract]
    ABSTRACT: Interferon-δ (IFN-δ) belongs to type I IFN. It was first identified by Lefevre in 1993. The porcine interferon-δ (PoIFN-δ)-related sequences were reported in 2009. The IFN-δ-related sequences have not been studied as closely as IFN-α and IFN-β. In this study, we identified 11 functional genes and 7 pseudogenes of the PoIFN-δ-related subtypes from the porcine genome database. Three of these genes were cloned, expressed, and functionally studied. PoIFN-δ-related subtypes can bind to the type I IFN receptors, activate the Janus kinase-signal transduce and activator of transcription (JAK-STAT) signaling pathway, induce IFN stimulate genes, and other cytokines. The antiviral activity of PoIFN-δ-related subtypes varies a lot in different subtypes. Among all the PoIFN-δ subtypes detected, PoIFN-δ8 has the strongest antiviral and immunomodulatory activities.
    No preview · Article · Jul 2012 · Journal of interferon & cytokine research: the official journal of the International Society for Interferon and Cytokine Research
Show more