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Effect of solvent fractions of kombucha tea on viability and invasiveness of cancer cells—Characterization of dimethyl 2-(2-hydroxy-2-methoxypropylidine) malonate …

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In the present study, cytotoxic and anti-invasive properties of solvent fractions of kombucha tea were studied. Kombucha tea was fractionated with chloroform, ethyl acetate and butanol. Three solvent extracts and the final aqueous phase were concentrated and used for anti-cancer study at 25, 50, 75 and 100 μg/mL concentrations. Results revealed that ethyl acetate fraction at a concentration of 100 μg/mL caused cytotoxic effect on 786-O and U2OS cells; reduced the activities of matrix metalloproteinase-2 (MMP-2) and-9 in 786-O cells and MMP-2 activity in A549 cells; and significantly reduced the cell invasion and cell motility in A549, U2OS and 786-O cells. Thus, ethyl acetate fraction was further purified by chromatographic studies and presence of dimethyl 2-(2-hydroxy-2-methoxypropylidene) malonate and vitexin were confirmed through IR, NMR and mass spectroscopic studies, which might be responsible for the observed anticancer property of kombucha tea.
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... Jurnal Biodjati,7(1), 86-94. rats, anti-inflammatory, hypocholesterolemic, anti-proliferative, and hypoglycemic activities (Villarreal et al., 2018), and anticancer (Jayabalan et al., 2011). ...
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Kombucha has an anticancer potency because it has dimethyl 2-(2-hydroxy-2-methoxypropilidine) malonate compound. The research aimed to verify the compound dimethyl 2-(2-hydroxy-2-methoxypropilidine) malonate as an anticancer with the in-silico method, namely the molecular docking approach, drug likeness profile, and ADMET test. The tools used were the PyRx, Discovery Studio Visualizer, Sanjeevini, and pkCSM. The research material consisted of 3D Dimethyl 2-(2-Hydroxy-2-Methoxypropilidine) Malonate and Epidermal Growth Factor Receptor (EGFR). The analysis showed Dimethyl 2-(2-Hydroxy-2-Methoxypropilidine) Malonate is safe for consumption and can suppress cancer cells
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