Production of (R)-1-phenylethanols Through Bioreduction of Acetophenones by a New Fungus Isolate Trichothecium roseum

Faculty of Sciences, Department of Chemistry, Ataturk University, Erzurum 25240, Turkey.
Chirality (Impact Factor: 1.89). 06/2010; 22(6):543-7. DOI: 10.1002/chir.20775
Source: PubMed


A total of 120 fungal strains were isolated from soil samples and evaluated in the bioreduction of substituted acetophenones to the corresponding (R)-alcohols. Among these strains, isolate Trichothecium roseum EBK-18 was highly effective in the production of (R)-alcohols with excellent enantioselectivity (ee > 99%). Gram scale preparation of (R)-1-phenylethanol is reported.

Download full-text


Available from: Mesut Taskin, Dec 04, 2015
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This database, available online, reviews 876 published accounts and presents a list of species representing the genera Aspergillus, Penicillium and related species in Turkey. Aspergillus niger, A. fumigatus, A. flavus, A. versicolor and Penicillium chrysogenum are the most common species in Turkey, respectively. According to the published records, 428 species have been recorded from various subtrates/habitats in Turkey.
    Full-text · Article · Jan 2004 · Mycotaxon -Ithaca Ny-
  • [Show abstract] [Hide abstract]
    ABSTRACT: Molecular chirality is a fundamental phenomenon that plays an important role in biological processes. Currently, much attention has been focused on the production of chiral alcohols. Chiral alcohols such as ( R )-1-(4-bromo-phenyl)-ethanol is important as a useful starting material used in pharmaceutical industries for drug synthesis. There are many factors that influenced the bioconversion and some of the crucial ones include the pH of media, biotransformation time, substrate concentration and agitation speed. All of these factors significantly affect the percentage of enantiomeric excess as well as the percentage conversion of the chiral alcohol. In this work, biocatalytic production of ( R )-1-(4-bromo-phenyl)-ethanol was achieved via asymmetric reduction of 1-(4-bromo-phenyl)-ethanone using the shake-flask method at the reaction conditions of 30° C, 1 mmol of substrate, agitation speed of 150 rpm and at various pH’s. Since Aspergillus niger can easily grow and produce enzyme that is suitable in converting the substrate, therefore, it was used as a biocatalyst in the reaction. Based on the results obtained, pH 7 gave the highest enantiomeric excess of 99.9% and conversion of 94.7% for the reduction time of 48 h.
    No preview · Article · Dec 2010 · Journal of Applied Sciences
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Dates by-products (discarded dates) from the sucrose-rich variety of ‘Deglet Nour’ were used as starting biomass to produce high-fructose syrup (HFS) based on an immobilized invertase process. A novel extracellular thermostable invertase obtained from Aspergillus awamori cultivated in submerged medium was induced with sucrose at 1% and used for this purpose. A zymogram of the crude extract showed the presence of a unique enzyme form that was optimally produced on the 5th day. This enzyme preparation was biochemically characterized and immobilized on acetic acid-solubilized chitosan by covalent binding using glutaraldehyde (Yi = 88%, Ya = 54% and 15.53 U/g). When deployed in a packed bed reactor (PBR), HFS was efficiently and continuously produced from sucrose derived from aqueous date extracts. Feeding with an extract initially containing 139.2 g/L total sugar with 78.6 g/L sucrose at a flow rate of 17 ml/h, 50°C and pH 6 resulted in a conversion factor of 0.95 and a final fructose content in the syrup of 69 g/L.
    No preview · Article · Nov 2011 · Biocatalysis and Biotransformation
Show more