ArticlePDF Available

Anti-microbial activity of sesame oil



The present study was designed to evaluate the anti-microbial activity of sesame oil against selected gram positive and gram negative microorganism. The minimum inhibitory concentration (MIC) study revealed that sesame oil shows that potent MIC value against Salmonella typhi is 10 µl/ml. But, for other organism the MIC values were in the range of 350-500 µl/ml. The sesame oil shows best antimicrobial activity with zone of inhibition range 15-25 mm and also equal with standard Kenamycin (19-40 mm) and also it shows highest zone of inhibition against Sal-monella typhi. From this study it has been concluded that sesame oil exhibited potent antimicrobial activity with selected microbial strains.
Saleem | Int. J. Res. Phytochem. Pharmacol. 2011, 1(1), 21-23
©JK Welfare & Pharmascope Foundation | International Journal of Research in Phytochemistry & Pharmacology 21
Anti-microbial activity of sesame oil
T.S. Mohamed Saleem*
Department of Pharmacology, Annamacharya College of Pharamcy, Rajampet 516126, India
The present study was designed to evaluate the anti-microbial activity of sesame oil against selected gram positive
and gram negative microorganism. The minimum inhibitory concentration (MIC) study revealed that sesame oil
shows that potent MIC value against Salmonella typhi is 10 µl/ml. But, for other organism the MIC values were in
the range of 350-500 µl/ml. The sesame oil shows best antimicrobial activity with zone of inhibition range 15-25
mm and also equal with standard Kenamycin (19-40 mm) and also it shows highest zone of inhibition against Sal-
monella typhi. From this study it has been concluded that sesame oil exhibited potent antimicrobial activity with
selected microbial strains.
Keywords: Anti-bacterial activity; cup plate method; minimum inhibitory concentration; Sesamum indicuum
Many efforts have been made to discover new antimi-
crobial compounds from various kinds of sources such
as micro-organisms, animals, and plants. One of such
resources is folk medicines. Systematic screening of
them may result in the discovery of novel effective
compounds (Tomoko et al., 2002). Contrary to the syn-
thetic drugs, antimicrobials of plant origin are not as-
sociated with many side effects and have an enormous
therapeutic potential to heal many infectious diseases
(Iwu et al., 1999).
In recent years, a large number of oils and their consti-
tuents have been investigated for their antimicrobial
properties against bacteria and fungi. Sesame (Sesa-
mum indicum L., Pedaliaceae) is a very old cultivated
crop and thought to have originated in Africa (Ram et
al., 1990). Chlorosesamone obtained from roots of se-
same has antifungal activity (Begum et al., 2000). Se-
same lignans have antioxidant and health promoting
activities (Kato et al., 1998). High amounts of both se-
samin and sesamolin have been identified in sesame
(Sirato-Yasumoto et al., 2001). Both sesamin and se-
samolin were reported to increase both the hepatic
mitochondrial and the peroxisomal fatty acid oxidation
rate. Sesame seed consumption appears to increase
plasma gamma-tocopherol and enhanced vitamin E
activity which is believed to prevent cancer and heart
disease (Cooney et al., 2001). The current research
presents an evaluation of antibacterial activity of se-
same oil and its inhibitory effect against common bac-
terial organism.
Sesame oil was obtained from local market and used
for this study. Microorganisms obtained from parent
The following microorganisms were used for antimi-
crobial activity. Bacillus subtilis (NCIM 2480), Staphylo-
coccus aureus (NCIM 2602), Escherichia coli (NCIM
2981), Salmonella typhi (NCIM 2493), Proteus vulgaris
(NCIM 2813), Cornebacterium diphtheria, Streptomyces
gresius. All the microorganisms were maintained at 4°
C on nutrient agar slants.
Determination of minimum inhibitory concentrations
(MIC) using macrodilution method
The Minimum Inhibitory Concentrations (MICs) of se-
same oil found to be active by the diffusion test were
determined based on the macrodilution method
(Berghe and Vlietinck, 1991) with some modifications
as follows. The sesame oil was serially diluted (two-
fold) in a series of test tubes using nutrient broth sup-
plemented with 10% glucose and 0.05% phenol red
(colour indicator). These were later inoculated with
0.2ml suspension of the test organisms. The final con-
centrations were in the range 1000 to 10 µl/mL in the
medium. Microbial growth was determined by observ-
ing for color change in the tube (red to yellow when
there is growth). The lowest concentration that
showed no change of color was considered as the MIC.
Antimicrobial activity
Cup plate method using Mueller-Hinton agar medium
was employed to study the preliminary antibacterial
activity of sesame oil against different microbial
strains. The agar medium was purchased from HI me-
dia Laboratories Ltd., Mumbai, India. Preparation of
Research Article
* Corresponding Author
Contact: +91-9542326252
Received on: 26-11-2010
Revised on: 30-11-2010
Accepted on: 5-12-2010
Saleem | Int. J. Res. Phytochem. Pharmacol. 2011, 1(1), 21-23
22 ©JK Welfare & Pharmascope Foundation | International Journal of Research in Phytochemistry & Pharmacology
nutrient broth, subculture, base layer medium, agar
medium and peptone water was done as per the stan-
dard procedure. The cups each of 9mm diameter were
made by scooping out medium with a sterilized cork
borer in a petri dish which was streaked with the or-
ganisms. The sesame oil (50 µL) was added separately
in the cups and petri dishes were subsequently incu-
bated. Kenamycin (30 µg) were used as standard refer-
ence drugs. Zone of inhibition produced by sesame oil
was measured in mm
Antimicrobial activity
The result of MIC of the sesame oil is shown in Table 1.
The lowest MIC of sesame oil (10 µl) was recorded
against Salmonella typhi. The MIC for other microor-
ganisms was found 350 µl for Cornebacterium diphthe-
ria, Bacillus subtillis, Staphylococcus aureus, Strepto-
myces gresius and 500 µl for Proteus vulgaris and
Escherichia coli. The antimicrobial activities of sesame
oil against the test microorganisms examined in this
study. The results are shown in Table 2. The sesame oil
exhibited strong antimicrobial effects against the test
microorganisms, with inhibition zones ranging from 15
to 25 mm. Notably, Salmonella typhi & Cornebacterium
diphtheria were more susceptible to the extract (inhibi-
tion zone: 24 & 25 mm respectively) compared to the
standard Kenamycin whose inhibition zones ranged
from 19-40 mm.
In this study we have reported the antimicrobial activi-
ty of sesame oil against gram positive and gram nega-
tive organism. The results of this study revealed that
MIC of sesame oil against Salmonella typhi is 10 µl/ml.
But, for other organism the MIC values were in the
range of 350-500 µl/ml. The sesame oil shows best
antimicrobial activity and also equal with standard Ke-
namucin and also it shows highest zone of inhibition
against Salmonella typhi. Anand et al. (2008) reported
that sesame oil is found to have the antibacterial activi-
ty against Streptococcus mutans, Lactobacilli acidophi-
lus, and total bacteria. This type of screening by using
sesame oil with very effective antibacterial and anti-
fungal activity could be promising agents for the future
more wide research.
From this study it has been concluded that sesame oil
exhibited potent antimicrobial activity with selected
microbial strains.
Anand DT, Pothiraj C, Gopinath RM, Kayalvizhi B. Effect
of oil-pulling on dental caries causing bacteria. Af J
Mic Res 2008;2:063-066.
Begum S, Furumoto T, Fukui H. A new chlorinated red
naphthoquinone from roots of Sesamum indicum.
Biosci Biotech Biochem 2000;64: 873-874.
Berghe VA, Vlietinek AJ. Screening methods for anti-
bacterial and antiviral agents from higher plants.
Methods for Plant Biochemistry 1991;6: 47-68.
Cooney RV, Custer LJ, Okinaka L, Franke AA. Effects of
dietary sesame seeds on plasma tocopherol levels.
Nutr. Cancer 2001;39: 66-71.
Iwu MW, Duncan AR, Okunji CO. New Antimicrobials of
Plant Origin. In: Janick J. (ed.): Perspectives on New
Crops and New Uses. ASHS Press, Alexandria, VA:
Kato MJ, Chu A, Davin LB, Lewis NG. Biosynthesis of
antioxidant lignans in Sesamum indicum seeds. Phy-
tochemistry 1998;47: 583-591.
Ram R, Catlin D, Romero J, Cowley C. Sesame: New
approaches for crop improvement. In: J. Janick and
J.E. Simon (eds.), Advances in new crops. Timber
Press, Portland, OR. 1990, pp. 225- 228.
Sirato-Yasumoto S, Katsuta M, Okuyama Y, Takahashi
Y, Ide T. Effect of sesame seeds rich in sesamin and
sesamolin on fatty acid oxidation in rat liver. J Agr
Food Chem 2001;49: 2647-2651.
Tomoko N, Takashi A, Hiromu T, Yuka I, Hiroko M, Mu-
nekazu I, Totshiyuki T, Tetsuro I, Fujio A, Iriya I, Tsu-
tomu N, Kazuhito W. Antibacterial activity of extracts
Table 1. MIC value of sesame oil
Micro organism
MIC value of
sesame oil in µl
Bacillus subtillis
Cornebacterium diph-
Proteus vulgaris
Salmonella typhi
Staphylococcus aureus
Streptomyces gresius
Escherichia coli
Table 2. Anti-microbial activity of sesame oil
Micro organism
Zone of inhibition
Bacillus subtillis
Proteus vulgaris
Salmonella typhi
Escherichia coli
Saleem | Int. J. Res. Phytochem. Pharmacol. 2011, 1(1), 21-23
©JK Welfare & Pharmascope Foundation | International Journal of Research in Phytochemistry & Pharmacology 23
preparated from tropical and subtropical plants on
methicillin-resistant Staphylococcus aureus. J Health
Sci 2002;48: 273276.
... On the other hand, in vitro study using the minimum inhibitory concentration (MIC) method on agar plate had revealed that sesame oil exhibited potent antimicrobial activity against S. typhi and E. coli when applied at concentrations of 10 μl/m and 500 μl/ml, respectively (Saleem, 2011). ...
... The antimicrobial effect of sesame oil or sesamol against S. aureus in food products is not studied previously, even though in vitro study on culture media conducted by Saleem (2011) using minimum inhibitory concentration (MIC) method revealed that sesame oil at concentration of 350 μl/ml exhibited a potent MIC value against S. aureus. Likewise, Alshahrani et al. (2020) in Saudi Arabia and Heidari-Soureshjani et al. ...
The antioxidant and antimicrobial effect of sesame oil (10, 30, and 50 g/kg) and sesamol (0.1, 0.3, and 0.5 g/kg) in meatballs during cold storage for 18 days at 3 ± 1 °C was investigated. Sesame oil and sesamol did not alter the sensory attributes of meatballs. Addition of either sesame oil or sesamol significantly delayed lipid oxidation when compared with control. Sesamol exhibited more potent antioxidant activities more than sesame oil. During storage, the aerobic plate counts (APCs) and Enterobacteriaceae counts (EBCs) were markedly (P < 0.01) decreased in meatballs treated with sesame oil or sesamol in comparison with untreated control samples. Control meatballs showed signs of quality deterioration at day 7 of storage, while treated meatballs exhibited longer shelf life to about 9–18 days according to sesame oil or sesamol concentrations. Both sesame oil and sesamol induced marked (P < 0.01) decline in E. coli O157:H7, Salmonella enterica serovar Typhimurium, Staphylococcus aureus and Listeria monocytogenes that artificially inoculated to meatballs. Sesamol was more effective than sesame oil in reduction of APC, EBC as well as foodborne pathogens. The results suggest that both sesame oil and sesamol are potentially useful natural additives to fresh meat products for improving its microbial quality and extend its shelf life during cold storage.
... The initial polyphenolic flavonoids extracted from fresh leaves of neem were quercetin and ß-sitosterol which were acknowledged to retain antifungal and antibacterial activities [33]. Rich organic and pharmacological properties have been recorded and comprise antibacterial [34], antifungal [35], and anti-inflammatory. Preceding investigators have recognized their role as anti-inflammatory, anti-arthritic, antipyretic, hypoglycemic, anti-gastric ulcer, antifungal, antibacterial and anti-tumour properties [36][37][38][39]. ...
... For detecting the microbiological quality of the examined beverages, the results showed that TBC were not detected in all treatments during storage. This result might be due to the carrot antimicrobial activity as reported by Babic et al., (1994), as well as the sesame and grape oil exhibited potent antimicrobial activity with selected microbial strains (Saleem, 2011, Ranjithav et al., 2014. The coliform counts in all beverage samples were <10 cfu / ml. ...
... The antibacterial mechanism of plant essential oils/extracts was generally suggested to be due to its major compounds especially polyphenolic compounds which is able to interact with cytoplasmic membrane of bacterial cells and subsequently cause the leakage of cellular components (26). Sesame seed lignans, such as sesamin, episesamin, sesamolin, sesaminol as well as sesamol, are known to inhibit the growth of bacterial (16,28,29). Lv et al., (30) also concluded that the mechanisms of combined antimicrobial interaction includes inhibition of the various biochemical pathway and critical enzymes and development of pores and cavities in bacterial cell membrane. ...
Various natural oils/extracts and their constituents incorporated into biopolymer-based edible films as a promising technology with the knowledge that these compounds have been able to reduce microbial growth and chemical changes of packed foodstuffs. The objective of this study was to evaluate the effect of incorporation of Ziziphora clinopodioides essential oil (ZEO; 0, 0.25 and 0.5%) and sesame oil (SO; 0, 0.5 and 0.75%) into chitosan-flaxseed mucilage (CH-FM) film against Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus and Escherichia coli O157:H7 in vitro condition and raw minced trout fillets during refrigerated condition. The in vitro antibacterial and antioxidant properties of CH-FM films were evaluated using agar disk diffusion method and free radical scavenging activity assay, respectively. The most important constituents of ZEO were found to be carvacrol (65.22%), thymol (19.51%), ɣ-terpinene (4.63%) and p-cymene (4.86%). The lowest and highest antimicrobial effect against S. aureus, L. monocytogenes, E. coli O157:H7 and S. typhimurium were found for CH-FM films enriched with SO 0.5% (0.98-1.24 mm) and ZEO 0.5% + SO 0.75% (5.01-6.25 mm), respectively. The antioxidant property of CH-FM based films were found to be ranged 5.45% ± 0.04-37% ± 0.45. In treated trout fillets, the counts of L. monocytogenes, S. aureus, E. coli O157:H7 and S. typhimurium were 1.54-4.18, 0.34-3.35, 0.29-1.45 and 0.19-1.27 log CFU/g significantly lower than control groups after two weeks of refrigerated storage, respectively. The designated films had good antibacterial effect against some food borne pathogenic bacteria including L. monocytogenes, S. aureus, S. typhimurium and E. coli O157:H7 in raw rainbow trout fillets.
... In a study by Saleem, it was found that the sesame oil has the antibacterial activity against Bacillus subtilis, Staphylococcus aureus, E. coli, Salmonella typhi, Proteus vulgaris, Corynebacterium diphtheriae, and Streptomyces griseus. [37] In this study, it was observed that the sesame oil has NI against dermatophytes and Candida. ...
Collection and documentation of indigenous knowledge of local people have an important role in scientific research, biodiversity conservation, and the drug development process. A study was carried out to document the medicinal plants that have been used by the local folk healer to treat Shingles in Ilam district, Eastern Nepal. A renowned folk healer who was involved in curing Shingles for decades and 30 key informants were selected for the interview to know the methods of curing Shingles. Altogether six plants viz: Oroxylum indicum, Cynodon dactylon, Centella asiatica, Drymaria cordata, Sesamum indicum, and Lygodium japonicum were found to be used against the disease. The traditional method of preparing medicine from these plants was found to be highly effective. The finding provides a clue for further extensive lab-based research to isolate the specific compounds that are effective against the disease.
Oil pulling has been used widely as a conventional Indian folk remedy for many years for strengthening teeth, gingiva, to prevent dental caries, halitosis, bleeding gums, dryness of mouth, and cracked lips. In the Ayurveda literature Charaka Samhita (Sutrasthana 5, 78–80), it is referred to as Gandoosha, Kavala, and Kavala Graha. It is claimed to treat about 30 systemic disorders ranging from diabetes to migraine and asthma. A Ukrainian medical practitioner, Dr. F. Karach, acquainted the remarkable notion of oil pulling in the 1990s. Recent studies on oil pulling therapy using sunflower and sesame oil were found to reduce dental caries and plaque‐induced gingivitis. The most amazing part of oil pulling therapy is that it can be performed using any cold pressed oil easily at home such as coconut, sunflower, or sesame oil; hence, it becomes a very cost‐effective modality. Since last decade, there are many studies available on the use of oil pulling for the maintenance of overall oral health. There is mounting evidence of oil pulling being as good as many other chemical‐containing ointments, toothpastes, or mouthwashes in control of oral problems with no untoward side effects. This chapter overviews the evidence‐based use of oil pulling therapy in the maintenance of oral health.
The ancient Egyptians believed in an afterlife where the body of a dead person would be needed by the spirit. In order to preserve the deceased in as lifelike condition as possible they developed artificial mummification to a high level of sophistication. It is widely believed that natron was the main desiccation agent in the preparation of Egyptian mummified bodies in the 18th Dynasty. Natron is a natural mixture of sodium chloride, sodium sulphate, sodium carbonate and sodium bicarbonate found in the Nile Delta which has significant desiccation properties, but it is currently unclear if natron has further beneficial properties for the mummification process. Previous work indicates that it does not deter insect colonisation in general, although it has been found to act as a killing agent of blow flies. In particular, we are aware of no studies investigating the antibacterial activity of natron nor the wider investigation of the insect repellent nature of ancient Egyptian mummification resins. This study investigated the properties of ancient Egyptian embalming fluids alongside their application in an artificial mummification of a human body at the Medico-legal Centre in Sheffield, UK. Antibacterial assays were used to compare the activity of a modern day antibiotic (Chloramphenicol) to those of key embalming ingredients used in ancient Egypt (natron, palm wine and pine resin). In addition, a field study using rabbit carcasses investigated whether pine resin has insect repellent properties. Results demonstrated that palm wine and natron had higher antibacterial activity than Chlorophemical against the Gram-positive bacteria, Staphylococcus aureus, and that natron had higher antibacterial activity than Chloramphenicol against the Gram-negative bacteria, Escherichia coli. The field study showed a delay in the colonisation of necrophagous insects and a temporal shift in the families of insects present on the carcasses treated with pine resin compared to control carcasses (no pine resin). We found no insect mortality on any of the carcasses during the study, indicating that the pine resin was acting as a repellent only and not as an insecticide. Although this is a preliminary study, the clear results strongly suggest that the embalming fluids used in mummification procedures during the 18th Dynasty had a number of properties which could affect the development of associated bacterial and insect communities.
With the growing demand for plant genetic resources in cosmetics industries, traditional herbal cosmetic knowledge is becoming a potential resource for innovation and economic development. However, despite the abundant ethnobotanical literature in Cameroon, the use of plants as cosmetics among ethnic groups has only been poorly investigated. This study was conducted to assess the traditional herbal cosmetic knowledge of the Gbaya ethnic group in the Eastern Cameroon. An ethnobotanical survey using a questionnaire was carried out and 59 Gbaya people aged between 20 and 90 years were interviewed. A total of 36 plants belonging to 26 families have been documented for their use as cosmetics or cosmeceuticals. The different cosmetic preparations were made from various plant parts of a single plant, or multiple plants, the seeds being the most frequently used organ (26.36% of citations). All these plants are known for their use to manage multiple skin, hair or teeth problems. Among them, Elaeis guineensis, Carica papaya, Aloe vera, Citrus limon and Baillonella toxisperma are the top five most frequent species. A total of 78 cosmetic recipes have been recorded including facial masks and scrubs, body cream, hair cream, and preparation for teeth hygiene and dandruff. The pharmacognostic review of these plants shows that they all contain diverse phytochemicals like enzymes, minerals, vitamins, alkaloids, phenolic compounds, steroids, saponins, glycosides, carbohydrates, coumarins, lecithin, and essential oils that are all active cosmetic ingredients. This study shows that the Gbaya people in East Region of Cameroon use various recipes for their tooth hygiene, skin and hair care. Promoting their sustainable use and the equitable sharing of benefits is a pathway for harnessing the conservation of these plants and the local development.
Full-text available
Infectious diseases account for approximately one-half of all deaths in tropical countries. In industrial-ized nations, despite the progress made in the understanding of microbiology and their control, incidents of epidemics due to drug resistant microorganisms and the emergence of hitherto unknown disease-causing mi-crobes, pose enormous public health concerns. Historically, plants have provided a good source of antiinfective agents; emetine, quinine, and berberine remain highly effective instruments in the fight against microbial in-fections. Phytomedicines derived from plants have shown great promise in the treatment of intractable infec-tious diseases including opportunistic AIDS infections. Plants containing protoberberines and related alkaloids, picralima-type indole alkaloids and garcinia biflavonones used in traditional African system of medicine, have been found to be active against a wide variety of micro-organisms. The profile of known drugs like Hydrastis canadensis (goldenseal), Garcinia kola (bitter kola), Polygonum sp., Aframomum melegueta (grains of para-dise) will be used to illustrate the enormous potential of antiinfective agents from higher plants. Newer drugs such as Xylopia aethiopica, Araliopsis tabouensis, Cryptolepis sanguinolenta, Chasmanthera dependens and Nauclea species will be reviewed.
Full-text available
The antibacterial activity of the extracts prepared from 181 species (75 families) of tropical and subtropi- cal plants was screened against various types of patho- genic bacteria. Among the 505 extracts tested, 53 of them inhibited the growth of methicillin-resistant Sta- phylococcus aureus (MRSA). The active extracts ob- tained from barks of Shorea hemsleyana and roots of Cyphostemma bainessi were separated to their compo- nents, some of which greatly reduced the viable cell number of MRSA. These active compounds were all identified as stilbene derivatives. Hemsleyanol D, one of the stilbene tetramer isolated from S. hemsleyana, was the most effective compound and had MIC of 2 µg/ ml.
Full-text available
The tocopherols, the major vitamers of vitamin E, are believed to play a role in the prevention of human aging-related diseases such as cancer and heart disease, yet little is known concerning determinants of their plasma concentrations. Evidence from animal studies suggests that the dietary source of gamma-tocopherol can significantly affect plasma levels of this tocopherol as well as its functional vitamin E activity. To determine whether plasma levels of tocopherols in humans are similarly altered, a study was undertaken in which subjects (n = 9) were fed muffins containing equivalent amounts of gamma-tocopherol from sesame seeds, walnuts, or soy oil. We observed that consumption of as little as 5 mg of gamma-tocopherol per day over a three-day period from sesame seeds, but not from walnuts or soy oil, significantly elevated serum gamma-tocopherol levels (19.1% increase, p = 0.03) and depressed plasma beta-tocopherol (34% decrease, p = 0.01). No significant changes in baseline or postintervention plasma levels of cholesterol, triglycerides, or carotenoids were seen for any of the intervention groups. All subjects consuming sesame seed-containing muffins had detectable levels of the sesame lignan sesamolin in their plasma. Consumption of moderate amounts of sesame seeds appears to significantly increase plasma gamma-tocopherol and alter plasma tocopherol ratios in humans and is consistent with the effects of dietary sesame seeds observed in rats leading to elevated plasma gamma-tocopherol and enhanced vitamin E bioactivity.
Sesame lignans, whose biosynthetic pathway is the subject of this study, have well-established antioxidant and health protecting properties. Using a combination of radio- and stable-isotopically labelled precursor administration experiments, it was demonstrated that E-coniferyl alcohol undergoes stereoselective coupling to afford (+)-pinoresinol in Sesamum indicum seeds. Only this enantiomer, and not its (−)-antipode, is metabolized further in maturing seeds to afford (+)-piperitol, (+)-sesamin, and (+)-sesamolin. Introduction of the methylene dioxy bridges occurs sequentially with piperitol first being formed, this being subsequently modified to afford sesamin.
The effect of oil-pulling on the reduction of total count of bacteria was determined. There was a remarkable reduction in the total count of bacteria. The process of oil-pulling reduced the susceptibility of a host to dental caries. The in-vitro antibacterial activity of sesame oil against dental caries causing bacteria was determined. Streptococcus mutans and Lactobacillus acidophilus were found to be moderately sensitive to the sesame oil.
A new chlorinated red naphthoquinone pigment having antifungal activity, named chlorosesamone, was isolated from the roots of Sesamum indicum. Its structure was characterized as 2-chloro-5,8-dihydroxy-3-(3methyl-2-butenyl)- 1,4-na phthoquinone on the basis of spectral evidence.
Activities of enzymes involved in hepatic fatty acid oxidation and synthesis among rats fed sesame (Sesamum indicum L.) differing in lignan content (sesamin and sesamolin) were compared. Sesame seeds rich in lignans from two lines, 0730 and 0732, lines established in this laborary, and those from a conventional cultivar (Masekin) were employed. Seeds from the 0730 and 0732 lines contained sesamin and sesamolin at amounts twice those from Masekin. Sesame seeds were added at levels of 200 g/kg to the experimental diets. Sesame increased both the hepatic mitochondrial and the peroxisomal fatty acid oxidation rate. Increases were greater with sesame rich in lignans than with Maskin. Noticeably, peroxisomal activity levels were >3 times higher in rats fed diets containing sesame seeds from the 0730 and 0732 lines than in those fed a control diet without sesame. The diet containing Masekin seed caused only a 50% increase in the value, however. Diets containing seeds from the 0730 and 0732 lines, compared to the control and Masekin diets, also significantly increased the activity of hepatic fatty acid oxidation enzymes including acyl-CoA oxidase, carnitine palmitoyltranferase, 3-hydroxyacyl-CoA dehydrogenase, and 3-ketoacyl-CoA thiolase. In contrast, diets containing sesame lowered the activity of enzymes involved in fatty acid synthesis including fatty acid synthase, glucose-6-phosphate dehydrogenase, ATP-citrate lyase, and pyruvate kinase. No significant differences in enzyme activities were, however, seen among diets containing sesame from Masekin cultivar and lines 0730 and 0732. Serum triacylglycerol concentrations were lower in rats fed diets containing sesame from lines 0730 and 0732 than in those fed the control or Masekin diet. It is apparent that sesame rich in lignans more profoundly affects hepatic fatty acid oxidation and serum triacylglycerol levels. Therefore, consumption of sesame rich in lignans results in physiological activity to alter lipid metabolism in a potentially beneficial manner.
New Antimicrobials of Plant Origin): Perspectives on New Crops and New Uses
  • Mw Iwu
  • Ar Duncan
  • Co Okunji
Iwu MW, Duncan AR, Okunji CO. New Antimicrobials of Plant Origin. In: Janick J. (ed.): Perspectives on New Crops and New Uses. ASHS Press, Alexandria, VA: 1999;457–462.