Propolis from Turkey induces apoptosis through activating caspases in human breast carcinoma cell lines
Celal Bayar University, Faculty of Medicine, Department of Histology and Embryology, 35040 Bornova, Izmir, Manisa, Turkey. Acta histochemica
(Impact Factor: 1.71).
10/2009; 112(6):546-56. DOI: 10.1016/j.acthis.2009.06.001
Propolis is a sticky substance that is collected from plants by honeybees that has anti-mutagenic and anti-carcinogenic properties with biological and therapeutic effects. The target of this study was to investigate the anti-apoptotic effect of propolis extracts (PE) on the caspase pathway in the human breast cell line MCF-7 in culture. Seven different propolis extracts, numbered PE 1-7, produced in their natural ecological environment, were collected from the Hacettepe University Beytepe Campus area in Ankara, Turkey. Individual extracts at 0.5, 0.25, 0.125 and 0.063mg/ml were incubated with MCF-7 cells during 2 days culture. Cell growth and cytotoxicity were measured colorimetrically by MTT assay. Apoptotic cell death was determined by the TUNEL method (terminal deoxynucleotidyltransferase-biotin nick end-labelling) and caspase activity was investigated by immunocytochemistry using antibodies directed against caspase 6, caspase 8 and caspase 9. The results showed that the PE 5 and 6 extracts at 0.125mg/ml dilution induced apoptosis in association with increased number of TUNEL positive cells. MTT results showed that cultures exposed to the same extracts and at the same dilution experienced better cell growth compared to those cultures exposed to the other extracts. Immunpositivity for all caspases was detected after treatment with all the extracts and at all dilutions, with stronger immunoreactivity for caspase 6 than caspases 8 and 9. Caspase 6 labelling was especially strong in PE 5 and PE 6. We conclude that propolis may have anti-tumour effects by increasing apoptosis through the caspase pathway. Such propolis extracts may be important economically and allow development of a relatively inexpensive cancer treatment.
Available from: Seema Patel
- "Prostate Iraqi Phenolic acids and their esters Colon Sulaiman et al. (2012) Blood Chinese Cinnamic acids and their esters Colon Ishihara, Naoi, Hashita, Itoh, & Suzui (2009) Greek Diterpenes (Manool) Colon Pratsinis, Kletsas, Melliou, & Chinou (2010) Cuban (brown) Nemorosone Breast Popolo et al. (2009) Popolo et al. (2011) Tunisian Myricetin, quercetin Lungs Kouidhi, Zmantar, & Bakhrouf (2010) Colon Turkish — Prostate Seda Vatansever et al. (2010) Barlak et al. (2011) Croatian Flavonoid aglycones Breast Sobočanec et al. (2011) Egyptian CAPE Breast Badr et al. (2011) Brazilian Artepillin C Breast Kamiya, Nishihara, Hara & Adachi (2012) Cinnamic acid derivatives (baccharin) Prostate Szliszka, Zydowicz, Mizgala, & Krol (2012) Endo et al. (2012) Mexican Phenylallylflavanones Pancreas Li, He, Awale, Kadota, & Tezuka (2011) Lungs Li et al. (2010) Fibroblast Portuguese Flavonoid Kidney Valente, Baltazar, Henrique, Estevinho, & Carvalho (2011) Oral Cavalcante et al. (2011) Caribbean Epi-nemorosone Prostate Diaz-Carballo et al. (2012) Indian — Breast, Colon Choudhari, Haghniaz, Raiwade, & Paknikar (2013) to enhance cytotoxicity of nymphaeol (Chen et al., 2012). Efficacy augmentation through adequate purification, supercritical extraction and substitution of functional groups must be emphasized. "
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ABSTRACT: Propolis is a bee-metabolized resinous substance (bee glue) from plant sap and gums. It has been in usage as a healing agent since antiquity, yet has not garnered global popularity as a health promoter. Its biological effects, which range from antimicrobial, antioxidant, anti-inflammatory, antidiabetic, dermatoprotective, anti-allergic, laxative and immunomodulatory to anticancer, have been validated. Propolis has shown efficacy against brain, head and neck, skin, breast, liver, pancreas, kidney, bladder, prostate, colon and blood cancers. The inhibition of matrix metalloproteinases, anti-angiogenesis, prevention of metastasis, cell-cycle arrest, induction of apoptosis and moderation of the chemotherapy-induced deleterious side effects have been deduced as the key mechanisms of cancer manipulation. The components conferring antitumor potentials have been identified as caffeic acid phenethyl ester, chrysin, artepillin C, nemorosone, galangin, cardanol, etc. These compounds target various genetic and biochemical pathways of cancer progression. Depending on the botanical sources and the geographical origin, biological activities of propolis vary. Despite phenomenal development in cancer research, conventional therapy falls short in complete malignancy management. The findings obtained so far build hope that propolis as a complementary medicine may address the lacunae. This review documents the recent advances and scope of amendement in cancer remediation with adequate emphasis on the mechanistic aspect of propolis.
Available from: Murtala Bello Abubakar
- "Propolis also known as bee glue is a dark resinous substance collected by the worker bees from various plant exudates which they use to fill spaces and to coat walls of the hive for protection  . It has a long history of medicinal use by humans  and has captured the attention of many researchers as a result of its antiviral, antifungal, antibacterial, anti-inflammatory, antioxidant, and anticarcinogenic properties                 . "
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ABSTRACT: Propolis (a bee product) which has a long history of medicinal use by humans has attracted a great deal of research interest in the recent time; this is due to its widely reported biological activities such as antiviral, antifungal, antibacterial, anti-inflammatory, antioxidant, and anticarcinogenic properties. Crude form of propolis and its phenolic contents have both been reported to exhibit antileukaemic effects in various leukaemia cell lines. The ability of the polyphenols found in propolis to arrest cell cycle and induce apoptosis and differentiation in addition to inhibition of cell growth and proliferation makes them promising antileukaemic agents, and hence, they are believed to be a key to the antileukaemic effects of propolis in different types of leukaemia. This paper reviews the molecular bases of antileukaemic activity of both crude propolis and individual polyphenols on various leukaemia cell lines, and it indicates that propolis has the potential to be used in both treatment and prevention of leukaemia. This however needs further evaluation by in vitro, in vivo, and epidemiological studies as well as clinical trials.
Available from: Khaled Mohamed Ahmed Mahran
- "It has several biological properties as antimicrobial, antiinflammatory, immunomodulatory , and antioxidant   . It have been used since ancient times as a medicine because of its biological properties as antiinflammatory, antiallergic properties, anticarcinogenic , antioxidative, antifungal, antiviral, immunostimulant , and for tissue regeneration   . Administration of propolis alleviated the harmful effects of insecticide, propetamphos . "
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ABSTRACT: The present experiment was conducted to study the effect of ethanolic extract of Egyptian propolis given alone or in combination with inactivated Pasteurella multocida vaccine on rabbits challenged with a virulent strain of Pasteurella multocida. Fifty-six New-Zealand rabbits, 6-8 weeks old and non-vaccinated against pasteurellosis, were randomly divided into eight equal groups. The first group was kept as a control for the experiment. The other groups received different treatments with propolis extract, inactivated vaccine, or both. The experiment continued for seven weeks during which clinical signs, body weight, and mortality rate were monitored, and blood samples were collected weekly for evaluating the leukogram, serum biochemistry, and immune response in all groups of animals. At the end of the seventh week, the animals were subjected to challenge with a virulent strain of Pasteurella multocida. Two weeks later, tissue specimens were collected from different organs for histopathological examination. Results showed that rabbits of the groups treated with both propolis and the vaccine by different routes appeared healthy after challenge. It has been concluded that alcoholic extract of propolis administrated in combination with inactivated Pasteurella multocida vaccine has no adverse effects on the general health conditions and enhances immune response in rabbits.
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