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In-vitro anti-denaturation and antibacterial activities of Zizyphus oenoplia
Abstract
Zizyphus oenoplia mill belongs to the family Rhamnaceae. The fruits of the plant are used as stomach-ache pills and root bark decoction is used to promote the healing of fresh wounds. Plant has shown the presence of cyclopeptide alkaloids. Aerial parts of the plant were reported to have anti-inflammatory and anticholinergic activities. Bark and leaves of the plant were reported for antibacterial activity. No biological and antibacterial activities were reported on root part of Zizyphus oenoplia. The aim of the study was to investigate the Anti-denaturation and antibacteriial activities for various extracts of the Zizyphus oenoplia. Root part of Zizyphus oenoplia was extracted with ethyl acetate, 90% ethanol and water using soxhlet apparatus. The solvents were evaporated under reduced pressure below 40 0 C using rotary evaporator to obtain dry extracts. These crude extracts were subjected to investigation for anti-denaturation study and antibacterial study by using a method designed by William's et al and agar ditch diffusion method respectively. Phytochemical analysis revealed the presence of carbohydrates, alkaloids, phenolic compounds, tannins and saponins. All the extracts of Zizyphus oenoplia protected Bovine Serum Albumin (BSA) from denaturation more than 97% at 1µg/ml concentration. Only ethanolic extract showed antibacterial activity against Staphylococus aureus.
... The effectiveness of using plant extracts to induce anti-denaturation effects in heat-treated bovine serum albumin (BSA) has been investigated as a potential parameter for evaluating the therapeutic potential of anti-in ammatory compounds, without requiring animal testing for initial pharmacological screening (Ramalingam et al., 2010). As far as we are aware, there is no existing information regarding the in vitro anti-denaturation properties of proteins found in Cucumis callosus. ...
Cucumis callosus is a member of the cucumber family and widely many regions of world for its underutilization vegetable and medicinal purposes. The present study focuses on assess biological activity of different extracts and evaluate the presence of phytochemicals through qualitative tests. Fruit powder extracted in soxhlet extractor using different organic solvent (Chloroform, Ethanol, Methanol, Petroleum ether). All four extracts study the anti- inflammatory activity, in vitro alpha – amylase inhibitory activity, anthelmintic activity and antibacterial activity. The qualitative analysis of different organic solvent extracts evaluated. Four different organic solvent out of this methanol solvent extract give comparatively show better biological. activity in protein denaturation and alpha – Amylase. C. callosus extracts have demonstrated significant alpha-amylase inhibitory activity, which could be useful in the management of diabetes. Methanolic extracts have shown potential anthelmintic activity against various intestinal parasites, which could be useful in the treatment of parasitic infections. Fruit extracts have exhibited antibacterial activity against several bacterial strains, which could be beneficial in the treatment of bacterial infections. All organic solvent presents the alkaloid, phenol and Tannins. The presence of these phytochemicals in the plant extract may contribute to their curing of disease. The present bioactive compounds in C. callosus it may be potential therapeutic properties make it a promising candidate for the development of new natural medicines for various diseases and conditions. However, further studies are needed to fully understand the mechanisms of action and cytotoxicity of C. callosus extracts.
... It's often utilized in province of Uttar Pradesh (India) for liver diseases [2,3]. The roots of the plant possess antiulcer and antioxidant, antihelminthic [4,5], antiplasmodial [6] angiogenic potential, antidenaturation and antibacterial activity [7], wound healing activity [8], hepatoprotective potential against antitubercular drugs induced hepatotoxicity [9] and as an ingredient in the preparation of stomach ache pills among the Munda tribe [10]. Aim of the current study to investigate Ziziphus oenoplia Mill preliminary phytochemical analysis, estimate the number of alkaloids present and identification of functional group present within the extracts using FTIR analysis. ...
... Antimicrobial: The antibacterial activities of Z. oenopolia were tested against wound pathogens caused skin infections. This antibacterial property leads to support the use of traditional medicine in the treatment of bacterial skin infections (David 2010). According to reported studies, the ethanol and methanol extract of Z. oenopolia showed activity against P. vulgaris and Enterobacter aerogenes respectively (Eswari et al. 2013). ...
Ziziphus oenopolia, which belongs to the Rhamnaceae family, is an important shrub often found throughout the warm regions of tropical Asia and northern Australia. It is used as a folk herbal medicine for various diseases, like abdominal pain and diarrhoea. It is a very popular wild edible fruit in tribal and rural areas. It also attracts many birds and insects. Due to rapid urbanization and anthropogenic activities, the population is declining. Therefore, keeping in mind the importance of these wild edible fruits, an attempt has been made to document their food, medicinal, ecological, and economic values through field and literature surveys to draw attention towards sustainable utilization of these wild nutraceuticals.
... Antimicrobial: The antibacterial activities of Z. oenopolia were tested against wound pathogens caused skin infections. This antibacterial property leads to support the use of traditional medicine in the treatment of bacterial skin infections (David 2010). According to reported studies, the ethanol and methanol extract of Z. oenopolia showed activity against P. vulgaris and Enterobacter aerogenes respectively (Eswari et al. 2013). ...
Plants are the major source of food and medicines in prehistory. The tribal people depend on the forest for their livelihood. They consume wild fruits, flowers, leaves, tubers, and bulbils. Among them, Indigofera cassioides, a deciduous shrub of the family Fabaceae, is a less explored medico-food plant, traditionally consumed as a wild edible flower by the tribals in many states of India. Less research is carried out on this wild edible flower. Therefore, an attempt has been made to present information on its food, medicinal, and economic values to draw attention to its potential for future nutraceuticals.
... Antimicrobial: The antibacterial activities of Z. oenopolia were tested against wound pathogens caused skin infections. This antibacterial property leads to support the use of traditional medicine in the treatment of bacterial skin infections (David 2010). According to reported studies, the ethanol and methanol extract of Z. oenopolia showed activity against P. vulgaris and Enterobacter aerogenes respectively (Eswari et al. 2013). ...
Following the COVID-19 pandemic, scientists and individuals worldwide began ingesting medicinal foods, or nutraceuticals, in an effort to boost immunity. In this regard, documentation of these wild nutraceuticals are crucial and significant for scientific endeavours.
This edited book, “Wild Nutraceutical Plants,” is an attempt made by the 5 editors and 26 authors to explore the knowledge of nutraceutical plants having pharmacological and economic values. The different species of plants that have been presented here possess many nutritional, medicinal, ecological, and economic values. The book will be helpful in the value addition of local nutraceuticals for sustainable utilization and conservation activities.
This book will be helpful to students, faculty, and researchers interested in learning about nutraceutical plants.
... The bacteria strains used in this study were selected based on those associated with gastrointestinal infections. Three bacteria species will be used and this will be pure cultures of E. coli, and patient isolate culture A. baumannii, before the culturing, during the antibacterial activity screening, the two bacteria were sub-cultured on nutrients for 24 hours to check the viability and as well to obtain fresh colonies that were still in the growth phase [6]. ...
The rise in the resistant strains has ignited the investigation of the ability of these essential oils to have an effect against them. The aim of this study was to investigate the antibacterial activity of clove oil, oregano oil and tea tree oil against Escherichia coli and resistant patient isolate Acinetobacter baumannii. Ciprofloxacin was used as standard and DMSO at 1 % was the negative control. To understand the inhibitory action on ATP synthase, docking analysis was done by using Autodock ver. 4.2.6. The study found out that essential oils had potent antibacterial activity against both E. coli and A. baumannii as all the essential oils recorded zones above 15 mm against E. coli and above 20 mm against A. baumannii. Tea tree oil had the highest activity among the three essentials against E. coli with 21.000±0.00 mm and against A. baumannii 28.500±0.500 mm respectively. These results support the antibacterial activity of essential oils. Docking analysis confirmed the ability of active constituents of selected essential oils to inhibit ATP synthase enzyme which is a crucial drug target in antimicrobial activity. Natural products have been at the center stage in the research for alternative agents with antibacterial agents.
The body's natural reaction is to heal a wound or an ulcer. In the event that there is no doshic invasion or infection, a wound will normally heal in one week. Dusta vrana(infected wound), which is defined as a vrana lasting longer than a week, infected with microorganism and that fails to heal for an extended length of time to produce a chronic wound, requires sthanika shodana. Materials and methods: Aragwadadhi kwatha dhavana was used for doing shodana in dusta vrana whom pseudomonas aeruginosa was isolated and the impact was observed. Conclusion:it was concluded that ARAGWADADHI kwatha dhavana was beneficial in destroying the gram-negative bacteria (pseudomonas aeruginosa).
The body's natural reaction is to heal a wound or an ulcer. In the event that there is no doshic invasion or infection, a wound will normally heal in one week. Dusta vrana(infected wound), which is defined as a vrana lasting longer than a week, infected with microorganism and that fails to heal for an extended length of time to produce a chronic wound, requires sthanika shodana. Materials and methods: Aragwadadhi kwatha dhavana was used for doing shodana in dusta vrana whom pseudomonas aeruginosa was isolated and the impact was observed. Conclusion:it was concluded that ARAGWADADHI kwatha dhavana was beneficial in destroying the gram -negative bacteria (pseudomonas aeruginosa).
BACKGROUND
The spine of Vallibadara [ Ziziphus oenoplia (L.) Mill.] of family Rhamnaceae are traditionally claimed to manage Mukhadushika (acne vulgaris). In Ayurveda, Avipattikara Churna is known for its Pitta Shamka and mild laxative action. The vitiation of Pitta Dosha and constipation are also contribute to manifestation of acne.
PATIENT INFORMATION AND INTERVENTION
A 19 year old patient presented with complaints of papules and pustules, scattered all over her forehead and cheeks since last one year. The patient also reported constipation and heartburn since many months. She was diagnosed with Mukhadushika and was treated with the local application of the paste of powdered thorn of Z. oenoplia mixed with lemon juice and oral administration of Avipattikara Churna 4 gm twice daily with water for 21 days.
RESULT
Considerable reduction was observed in the scattered papules and pustules on the forehead and cheeks of the patient. Constipation and burning sensation in the chest were relieved after one week. Complete relief in symptoms was noticed after 21 days of treatment. No recurrence of eruptions was observed during the follow-up after 30 days.
CONCLUSION
The external application of spine of Z. oenoplia and internal administration of Avipattikara Churna are effective in the treatment of acne vulgaris.
Background
Holarrhena has been traditionally used to treat inflammatory disorders and oxidative stress. The purpose of our study was to examine the biological potential of phytoconstituents found in the stem bark of H. pubescens using antioxidant and anti-inflammatory in-vitro methods.
Method
Dried stem bark of this plant was firstly extracted with n-hexane for defatting and then extracted with hydro-alcohol (90% ethanol). The hydro-alcoholic extract was further fractionated by different solvents (chloroform, ethyl acetate and butanol) and the antioxidant activity was assessed using reducing power assay, free radical scavenging methods for 1,1-diphenyl-2-picrylhydrazyl (DPPH), and hydrogen peroxide radicals. Anti-inflammatory activities were carried out by using albumin denaturation and nitric oxide (NO) assays.
Results
The qualitative phytochemical analysis of n-hexane and hydro-alcoholic extract revealed the presence of alkaloids, saponins, flavonoids, phenols, carbohydrates, proteins and tannins. In quantitative phytochemical analysis of hydro-alcoholic extract showed a higher percentage of phytochemicals than n-hexane extract. Hydro-alcoholic extract and fractions showed significant antioxidant activities against DPPH, H2O2, and reducing power assay. Similarly, in anti-inflammatory studies, butanol fraction showed higher protection against albumin denaturation which was 55.81 ± 2.05 whereas in NO assay ethyl acetate fraction was 30.39 ± 1.37.
Conclusion
Our research concluded that the hydro-alcoholic extract has highly phytochemical properties than n-hexane extract whereas ethyl acetate and butanol fraction showed superior anti-oxidant and anti-inflammatory properties as compared to other fractions. This research supports the plant's traditional use as a medicine.
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