Mesenchymal stem cells regulate the proliferation and differentiation of neural stem cells through Notch signaling

Institute of Urology, Nanchang University, Nanchang 330006, China.
Cell Biology International (Impact Factor: 1.93). 09/2009; 33(11):1173-9. DOI: 10.1016/j.cellbi.2009.08.004
Source: PubMed


The effects of mesenchymal stem cells (MSCs) on proliferation and cell fate determination of neural stem cells (NSCs) have been investigated. NSCs were co-cultured with MSCs or NIH3T3 cells using an in vitro transwell system. After 4 days, immunofluorescence staining showed that the number of cells positive for the cell proliferation antigen, ki-67, in neurospheres in MSCs was greater than in NIH3T3 cells. In some experiments, the top-layers of MSCs and NIH3T3 cells were removed to induce NSCs differentiation. Seven days after initiating differentiation, the levels of the neuronal marker, NSE, were higher in NSCs in MSCs co-culture group, and those of glial fibrillary acidic protein (GFAP) were lower, compared with NIH3T3 cells co-culture group. These were confirmed by immunofluorescence. The role of the Notch signaling pathway analyzed with the specific inhibitor, DAPT, and by examining the expression of Notch-related genes using RT-PCR showed that after co-culturing with MSCs for 24h, NSCs expressed much higher levels of ki-67, Notch1, and Hes1 than did NSCs co-cultured with NIH3T3 cells. Treatment with DAPT decreased ki-67, Notch1 and Hes1 expression in NCSs, and increased Mash1 expression. The data indicate that the interactions between MSCs and NSCs promote NSCs proliferation and are involved in specifying neuronal fate, mediated in part by Notch signaling.

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Available from: Yang Wang, Oct 03, 2015
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    • "In other systems, Notch signaling acts to coordinate proliferation and differentiation, as observed, for example, with mammalian Notch1, which is necessary for both the proliferation of neuronal stem cell populations and their differentiation into specific neuronal types (Hitoshi et al. 2002; Wang et al. 2009; Ables et al. 2010; Zhou et al. 2010; Matsumoto et al. 2011). We showed that in C. elegans, Notch can act not only as a factor in specifying cell types, but also, through an apparently distinct process, in the general restriction of developmental plasticity. "
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