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Antimicrobial Activity of Gentiana lutea L. Extracts



Methanolic extracts of flowers and leaves of Gentiana lutea L., together with the isolated compounds mangiferin, isogentisin and gentiopicrin, were used to investigate the antimicrobial activity of the plant. A variety of Gram-positive and Gram-negative bacteria as well as the yeast Candida albicans has been included in this study. Both extracts and isolated compounds showed antimicrobial activity with MIC values ranging from 0.12-0.31 mg/ml. Our study indicated that the synergistic activity of the pure compounds may be responsible for the good antimicrobial effect of the extracts. Quantification of the secondary metabolites was performed using HPLC.
0939 5075/2009/0500 0339 $ 06.00 © 2009 Verlag der Zeitschrift für Naturforschung, Tübingen · · D
The roots of Gentiana lutea L. (Gentianaceae),
a yellow fl owering plant commonly found in the
mountain regions of central and south Europe,
are very popular as a stomachic as well as a com-
ponent in preparations showing benefi cial effects
in gall and liver diseases (Wichtl, 1994). The ac-
tive principles are the bitter tasting secoiridoid
glycosides gentiopicrin and amarogentin. Some
investigations pointed out an interesting chemi-
cal composition of the aerial parts of G. lutea.
The presence of the xanthone isogentisin and two
avone heterosides was reported in leaves of G.
lutea (Hostettmann et al., 1973). The secoiridoids
gentiopicrin and swertiamarin, the xanthones
mangiferin, isogentisin and isogentisin-3-O-pri-
meveroside, and the fl avones isoorientin and iso-
vitexin have been isolated from the aerial parts of
G. lutea (Menkovic´ et al., 2000).
The development of resistance by pathogens to
many of the commonly used antibiotics provides
a stimulus for further attempts to search for new
antimicrobial agents to combat infections and
overcome problems of resistance and side effects
of the currently available antimicrobial agents.
Antibacterial effects of G. lutea roots have been
described recently, and it was shown that the
dry extract was effective against Streptococcus
pyogenes (Weckesser et al., 2007). Other studies
indicated that G. lutea exhibits antimicrobial ef-
fects that correspond to the effect of ampicillin
and it could be used in the treatment of bacterial
infections (Stierna et al., 2005). As for the antimi-
crobial activity of leaves and fl owers extracts is
concerned, only an antitubercular effect against
Mycobacterium bovis was reported (Menkovic´ et
al., 1999). The aim of the present study was to
investigate the antimicrobial activity of G. lutea
leaves and fl owers extracts and the isolated com-
pounds gentiopicrin, isogentisin and mangiferin
against various bacteria and the yeast Candida
Material and Methods
Plant material
Leaves and fl owers of Gentiana lutea were col-
lected at mountain Suvobor (at a height of ca.
830 m), Serbia, in July 2006. A voucher specimen
(17506) has been deposited in the herbarium of
the Botanic Garden “Jevremovac”, Faculty of Bi-
ology, University of Belgrade, Serbia.
Sample preparation
Air-dried leaves and fl owers were extracted
separately with methanol (1000 ml) in a Soxhlet
apparatus for 24 h and the solvent was evapo-
rated. Dry extracts of leaves (17.5 g) and fl owers
(22.3 g) were used for the experiments.
Antimicrobial Activity of Gentiana lutea L. Extracts
Katarina Šavikin*, Nebojša Menkovic´, Gordana Zdunic´, Tatjana Stevic´,
Dragoja Radanovic´, and Teodora Jankovic´
Institute for Medicinal Plants Research, Tadeuša Košc´uška 1, 11000 Belgrade, Serbia.
Fax: +38 11 13 03 16 49. E-mail:
* Author for correspondence and reprint requests
Z. Naturforsch. 64 c, 339 342 (2009); received November 14/December 26, 2008
Methanolic extracts of fl owers and leaves of Gentiana lutea L., together with the isolated
compounds mangiferin, isogentisin and gentiopicrin, were used to investigate the antimicro-
bial activity of the plant. A variety of Gram-positive and Gram-negative bacteria as well
as the yeast Candida albicans has been included in this study. Both extracts and isolated
compounds showed antimicrobial activity with MIC values ranging from 0.12 0.31 mg/ml.
Our study indicated that the synergistic activity of the pure compounds may be responsible
for the good antimicrobial effect of the extracts. Quantifi cation of the secondary metabolites
was performed using HPLC.
Key words: Gentiana lutea, Antimicrobial Activity, Gentiopicrin
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340 K. Šavikin et al. · Antimicrobial Activity of Gentiana lutea
HPLC conditions
Analyses were carried out on a HP series
1090 instrument with a DAD detector, on a re-
verse phase Zorbax SB-C18 analytical column
[150 × 4.6 mm i.d., particle size 5 μm (Agilent)].
Mobile phase A was H
O containing 1% 0.1
, mobile phase B was MeCN. Gradient
elution was according to the following scheme:
98 – 90% A, 0 – 5 min; 90% A, 5 – 10 min; 90 – 85%
A, 10 – 13 min; 85% A, 13 – 15 min; 85 – 70% A,
15 – 20 min; 70 – 40% A, 20 – 24 min; 40 – 0% A,
24 – 28 min; fl ow at 1 ml/min; detection at 260 and
320 nm. The xanthones mangiferin and isogenti-
sin, and the secoiridoid gentiopicrin were isolated
according to the previously published procedure
(Menkovic´ et al., 2000). Quantifi cation was per-
formed using HPLC and the amounts of the com-
pounds were calculated using calibration curves.
All experiments were repeated at least three
times. The results are presented as mg/g of dry
weight (dw).
Studied activity
The antimicrobial activity was tested against
six Gram-negative (Escherichia coli, Salmonella
typhimurium, S. enteritidis, Pseudomonas aerugi-
nosa, P. tolaasii, Enterobacter cloacae) and nine
Gram-positive bacteria (Staphylococcus aureus, S.
epidermidis, Streptococcus faecalis, Bacillus subti-
lis, Micrococcus luteus, M. avus, Proteus mirabi-
lis, Sarcina lutea, Listeria monocytogenes), as well
as one human pathogen yeast (Candida albicans).
The MIC (minimum inhibitory concentration)
values were determined using the broth microdi-
lution method in 96-hole plates according to NC-
CLS (2000). Serial dilutions of the stock solutions
of test extracts in broth medium (Muller-Hinton
broth or Sabouraud broth) were prepared in a
microtiter plate. The microbial suspensions were
added in the microwells at the concentration of
5 · 10
organisms/ml. The MIC values were de-
termined as the lowest concentrations preventing
visible growth. Streptomycin and nystatin were
used as a positive control. Each assay was repeat-
ed independently two times.
Results and Discussion
The HPLC profi les of G. lutea leaves and fl ow-
ers methanolic extracts are shown in Fig. 1. The
amounts of mangiferin and gentiopicrin were
nearly similar in leaves and fl owers, but the
amount of isogentisin was about ten times higher
in fl owers (Table I).
The results of the antimicrobial activity deter-
mination of G. lutea extracts and isolated com-
pounds are presented in Table II. Leaves and
owers extracts inhibited the growth of 15 of
16 pathogenic microorganisms tested, only the
Gram-positive bacterium Listeria monocytogenes
was resistant and has grown at the highest ap-
plied concentrations of both extracts. The MIC
values of the leaves extract were between 0.12
and 0.31 mg/ml, and the most sensitive to this ex-
tract were Pseudomonas aeruginosa, Bacillus sub-
tilis, Proteus mirabilis, Staphylococcus epidermidis
and Candida albicans. The fl owers extract exerted
slightly lower antimicrobial activity and the most
susceptible microorganism was Salmonella enteri-
tidis (MIC 0.15 mg/ml).
Among the individual extract components, the
compound with the widest spectrum of activity
was found to be gentiopicrin. It was most active
against Escherichia coli (0.12 mg/ml) and showed
moderate activity against Salmonella typhimu-
rium and Staphylococcus aureus (0.15 mg/ml).
The obtained results, along with published data,
characterize gentiopicrin as a natural compound
with a broad antimicrobial effect (Kumarasamy et
al., 2003; Nadinic et al., 2002). The antimicrobial
activity of the xanthone isogentisin against My-
cobacterium bovis has been reported previously
(Menkovic´ et al., 1999). In the present study, iso-
gentisin showed moderate antimicrobial activi-
ties with MIC values between 0.15 and 0.31 mg/
ml. Among the Gram-negative bacteria the most
susceptible were E. coli and Pseudomonas aeru-
ginosa. The species most sensitive to isogentisin
among the Gram-positive bacteria was Micro-
coccus luteus. Compared to the other examined
compounds, mangiferin showed lower antibacte-
rial activity with MIC values between 0.20 and
0.31 mg/ml. The antimicrobial activity of mangif-
Table I. The amount of secondary metabolites in G.
lutea leaves and fl owers extracts.
Sample Gentiopicrin
[mg/g dw] Mangiferin
[mg/g dw] Isogentisin
[mg/g dw]
Leaves 38.85 ± 0.7 9.57 ± 0.4 12.86 ± 0.7
Flowers 48.38 ± 1.4 8.98 ± 0.4 123.23 ± 3.1
Mean ± s.d. (n = 3).
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K. Šavikin et al. · Antimicrobial Activity of Gentiana lutea 341
Table II. Antimicrobial activity (MIC values in mg/ml) of G. lutea leaves and fl owers extracts and isolated com-
Microorganism Extract 1 Extract 2 MG IG GP Streptomycin
Bacillus subtilis ATCC 6051 0.12 0.19 0.27 0.19 0.19 0.0052
Listeria monocytogenes ATCC 15313 0.31 0.31 0.31 0.22 0.27 0.016
Micrococcus fl avus ATCC 10786 0.31 0.22 0.22 0.22 0.27 0.0052
Micrococcus luteus ATCC 10240 0.15 0.19 0.20 0.15 0.31 0.016
Proteus mirabilis ATCC 14273 0.12 0.19 0.20 0.19 0.19 0.0052
Sarcina lutea ATCC 10054 0.27 0.19 0.31 0.31 0.22 0.038
Staphylococcus aureus ATCC 25932 0.15 0.22 0.31 0.22 0.15 0.0052
Staphylococcus epidermidis ATCC 12228 0.12 0.19 0.27 0.22 0.22 0.0052
Streptococcus faecalis ATCC 12952 0.27 0.22 0.22 0.19 0.19 0.027
Escherichia coli ATCC 25922 0.15 0.19 0.20 0.15 0.12 0.0052
Enterobacter cloacae ATCC 13883 0.22 0.27 0.27 0.31 0.19 0.038
Pseudomonas aeruginosa ATCC 27853 0.12 0.27 0.27 0.15 0.22 0.016
Pseudomonas tolaasii NCTC 387 0.27 0.27 0.27 0.31 0.22 0.027
Salmonella typhimurium ATCC 14028 0.15 0.19 0.22 0.19 0.15 0.038
Salmonella enteritidis ATCC 13076 0.19 0.15 0.22 0.19 0.19 0.016
Candida albicans ATCC 10231 0.12 0.22 0.31 0.27 0.27 0.0052*
Extract 1, methanolic extract of G. lutea leaves; extract 2, methanolic extract of G. lutea owers; MG, mangiferin;
IG, isogentisin; GP, gentiopicrin.
5 10 15 20 25 30
G. lutea, leaves
5 10 15 20 25 30
G. lutea, flowers
Fig. 1. HPLC chromatograms of methanolic extracts of G. lutea leaves and fl owers.
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342 K. Šavikin et al. · Antimicrobial Activity of Gentiana lutea
erin against several bacterial species has been re-
ported previously (Stoilova et al., 2005).
Our study indicated that each tested compound
did not possess a dominant role in the antimicro-
bial activity of crude extracts. Thus, synergistic ac-
tivity may be responsible for the inhibitory effect
of the extracts. Since crude extracts of G. lutea
leaves and fl owers showed a wide range of an-
timicrobial effect, their use in the treatment of
various bacterial and fungal infections could be
benefi cial.
The authors acknowledge their gratitude to the
Ministry of Science of Serbia for fi nancial support
(project number TR 6846B).
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... A high concentration of active compounds in flowers was previously recorded for methanol extracts [30], where the concentration of isogenistin was 10 times higher in flowers than in leaves. The high content of naringenin and hesperidin has been previously reported for Gentiana bitter liquors and by-products [31], where the concentration of naringenin and hesperidin were higher in by-products compared to the liquor. ...
... Anionic substances can remove cations from the binding sites of LPs disrupting the membrane activ-ity. It was suggested that chlorogenic acid, due to its negative surface charge, binds to the outer membrane disrupting the membrane and leading to the loss of barrier function [30], which is related to data presented in this study. However, plant extracts may contain hundreds or even thousands of components, and identifying the compounds responsible for a given biological effect represents a significant challenge. ...
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Gentiana lutea L. is a medicinal plant the roots of which are primarily used in treatments of various human digestive disorders, but also the production of alcoholic liquors. The roots (radix) of G. lutea are described in the European Pharmacopeia, but knowledge about the chemical composition and biological activities of its aerial parts is still limited. Thus, until today aerial parts of this species have not been used in medical treatments or consumed. Therefore, flowers, leaves, and stems extracts of G. lutea obtained by using four different extraction solvents (petrol ether, chloroform, ethanol, and water) were examined for their chemical composition and biological activities. High concentrations of salicylic acid, apigenin, and naringenin were recorded for ethanol stem extracts, while significant amounts of kaempferol were detected in leaves and flowers in chloroform and water extracts, respectively. The highest antioxidant potential was recorded for flower and stem petrol ether extracts with the lowest IC50 values, ranging from 94.46 ± 9.45 to 105.38 ± 10.54 μg/mL. Ethanol extracts of flowers and stems showed moderate antioxidant activity (IC50 143.15 ± 14.32 and 146.90 ± 14.69 μg/mL) as well as strong antimicrobial activity against Candida albicans (21.00 ± 1.00 and 27.50 ± 1.78 mm inhibition zones, respectively). In addition, ethanol extracts had higher antimycotic activity compared to naturally occurring phenolic compounds that are used as positive controls. Moreover, statistical analysis of the activities of plant extracts and single compounds showed that levels of chlorogenic and caffeic acids strongly correlate with the biological activities of the extracts, i.e., they are the main carriers of these biological activities. The presented results indicate the possible use of aerial parts of G. lutea as a natural preservative, as well as a antimicrobial agent, which significantly amplifies the benefits of this medicinal crop and greatly affects the sustainability of cultivated Gentiana plantation.
... Several studies have reported that G. lutea and its bitter agents exert an antimicrobial action. In this regard, it was observed that the effect of gentian corresponded to that of the antibiotic ampicillin, which is used to treat different grampositive and negative bacterial infections, including meningitis, endocarditis, salmonellosis, respiratory and urinary tract infections (Brogden et al., 1979;Š avikin et al., 2009b). ...
Over many years, natural products have been a source of healing agents and have exhibited beneficial uses for treating human diseases. The Gentiana genus is the biggest genus in the Gentianaceae, with over 400 species distributed mainly in alpine zones of temperate countries around the world. Plants in the Gentiana genus have historically been used to treat a wide range of diseases. Still, only in the last years has particular attention been paid to the biological activities of Gentiana lutea Linn., also known as yellow Gentian or bitterwort. Several in vitro/vivo investigations and human interventional trials have demonstrated the promising activity of G. lutea extracts against oxidative stress, microbial infections, inflammation, obesity, atherosclerosis, etc.. A systematic approach was performed using Pubmed and Scopus databases to update G. lutea chemistry and activity. Specifically, this systematic review synthesized the major specialized bitter metabolites and the biological activity data obtained from different cell lines, animal models, and human interventional trials. This review aims to the exaltation of G. lutea as a source of bioactive compounds that can prevent and treat several human illnesses.
... Mango extract contain gallotannin and mangiferin. Presenting of these chemicals, extracts shows antibacterial activity against both Gram-positive and Gram-negative bacteria [6,7,8,9] . Mangiferin (Mangiferin is a xanthonoid found in mangoes) is able to interfere with the assembly or functioning of microtubule filaments or cellular matrix components or inhibition of the telomerase and the gene. ...
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Abstract Mango is antioxidant rich one of the most popular fruits in the world. Mangoes (Mangifera indica)belong to genus Mangifera under the family of Anacardiaceae. In Bangladesh, Mango is called as the“King of fruit” over a large area, but locally known as “aam”. Mango contains different types ofchemicals and most of them are bioactive compounds such as polyphenols, vitamin C, carotenoids,anthocyanin, and flavonoids. The present study was to extract the chemicals from Langra mango as wellas investigated the antimicrobial properties by using various in vitro methods. The mature fruit wasextracted with methanol. Chemicals from mango pulps were extracted by using Rotary evaporator.Phytochemical tests were performed to investigate the possible phytochemicals present in the mangopulp extract. Antimicrobial activity and Minimum Inhibitory Concentration (MIC) were performed bydisc diffusion and broth dilution assay against five (5) pathogenic bacteria. The antimicrobial activityexerted by the extract was compared with standard and results showed that extract contained significantantimicrobial activity. The order of zone of inhibition was Staphylococcus aureus (17mm) > Bacillussubtilis (16 mm) > Escherichia coli (14 mm) > Salmonella typhi (14 mm) > Klebsiella pneumoniae (12mm). The MIC values were in the range 0.6 mg/ml to 1.25 mg/ml for extract of Langra mango pulp. Thelowest MIC was found for Escherichia coli (0.6 mg/ml). So the extract (methanolic) of langra mangopulp has potent antimicrobial properties. (PDF) Antimicrobial activity of methanolic extract of langra mango pulp. Available from: [accessed Dec 17 2022].
... 7 Similar statement is also given by the European Scientific Cooperative on Phytotherapy (ESCOP) who defined "loss of appetite e.g., after illness and dyspeptic complaints" as therapeutic indications. 8 Beside the positive effect on the gastrointestinal tract, a variety of other pharmacological activities have been described for gentian root, including antioxidant, 9−11 antimicrobial, 12 antibacterial, 13,14 antifungal, 15 hepatoprotective, 16 antiatherosclerotic, 17 wound-healing, 18 immunological, 19 and secretolytic 20 effects. These various effects are attributed not only to the contained bitter-tasting secoiridoid glycosides but also to further secondary metabolite classes. ...
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This study presents the first ultra-high performance supercritical fluid chromatography-diode array detector (UHPSFC-DAD) assay for simultaneous quantitation of secoiridoids, iridoids, xanthones, and xanthone glycosides in Gentiana lutea L. Separation was reached within 12 min on an Acquity UPC2 BEH 2-EP column using CO2 and methanol with 5.5% water as mobile phases. Method validation for nine selected marker compounds (gentisin, isogentisin, swertiamarin, sweroside, gentiopicroside, loganic acid, amarogentin, gentioside, and its isomer) confirmed the assay's sensitivity, linearity, precision, and accuracy. The practical applicability was proven by the analysis of 13 root specimens and 10 commercial liquid preparations (seven liqueurs and three clear spirits). In all root batches, the secoiridoid gentiopicroside dominated (2.1-5.6%) clearly over all other metabolites. In the liqueurs, the metabolite content and distribution were extremely variable: while gentiopicroside was the main compound in four liqueurs, sweroside dominated in one preparation and loganic acid in two others. In contrast, measurable amounts of the metabolites were not detected in any of the examined clear spirits.
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... Among all tested extracts, the willow gentian showed the highest antimicrobial activity toward C. albicans ( Table 2), while the concentration of polyphenols in this plant extract is lower than in all other plant extracts. Besides polyphenols, the major compounds in willow gentian are secoiridoids (gentiopicrin), and it has been reported that the gentiopicrin inhibited the growth of Gram-negative, Gram-positive pathogen bacteria, as well as yeast C. albicans (46). Additionally, the predominant constituents of St. John's wort besides polyphenols are naphthodianthrones (hypericin and pseudohypericin) and phloroglucinols (hyperforin and adhyperforin) (26). ...
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Medicinal plants and their extracts contain substantial quantities of polyphenols. As metabolically active plant metabolites, polyphenols are food components with a wide range of biological activities. Given their poor absorbability in the digestive tract their activity toward the human host is typically mediated through interaction with intestinal microbes. As a result, polyphenols comprise a novel group of prebiotics. In this study, we tested the effect of five polyphenol-rich extracts from four medicinal herbs on the growth of probiotic and pathogenic microbes. The studied medicinal herbs were Gentiana asclepiadea L. (willow gentian), Hypericum perforatum L. (St. John's wort), Satureja montana L. (winter savory), and Achillea millefolium L. (yarrow). All these plants are traditionally used for the treatment of digestive problems. Extracts were prepared using safe solvent combinations. We tested the impact of addition of plant extracts on the growth of three probiotic lactobacilli and probiotic yeast Saccharomyces boulardii . The effect of addition of plant extracts to liquid media (concentration range 0.25–10 mg/mL) on the growth of probiotics, was tested in vitro . The antimicrobial activity of the extracts was tested against several opportunistic bacteria and yeast. St. John's wort, winter savory, and willow gentian extracts showed a stimulative effect on probiotic yeast growth, while the highest growth-stimulating effect was achieved when microwave-assisted yarrow extract was used in the concentration of 0.5 mg/mL. Under these conditions growth of S. boulardii was increased 130-fold. In addition, the yarrow extract stimulated the growth of Lactiplantibacillus plantarum 299v. The growth of two Lacticasibacillus rhamnosus strains was not stimulated by the addition of any extracts. Our results show that plant polyphenol-rich extracts can influence the growth of microorganisms that are typical members of the intestinal microbiota. For the first time we demonstrate that probiotic yeast growth can be stimulated by extracts of medicinal herbs, which when accompanied by suppression of Candida yeasts suggests a potential benefit of the treatment in diseases that are associated with fungal dysbiosis.
Protein kinase G (PknG), a eukaryotic-like serine-threonine protein kinase produced by pathogenic mycobacteria to prevent mycobacteria from degradation by lysosomes, is a critical virulence factor for intracellular survival of Mycobacterium tuberculosis. The practice of daily Hawan (Yajña) has been found to alter the microbiome by eradicating harmful microorganisms. Hawan is an aroma therapy of Āyurveda that evolve a large number of bioactive components having various pharmacological potentials. We have docked ten principal components of oblation materials (Hawan Sāmagri) Guggulsterone (Guggul), Magniferin (Mango leaves), α-santalol (Sandal wood), Patchoulol (Jatāmāsi), 2-propyl-4-methylthaizole (Sesame seeds), Eugenol (Clove), Myristicin (Nutmeg), Sesquiterpenes (Nāgkesar), Valerenic (Tagar) and Crocetin (Saffron) with protein structure PknG of M. tuberculosis by CB dock blind tool. This present investigation demonstrates the inhibitor binding positions and affinity utilizing glide-score. All the components tend to cure and inhibit the function of M. tuberculosis decreasing the risk of resistance. Out of nine components, Guggulsterone showed the best binding potential.
Gentiana species belonging to the Gentianaceae family are medicinal plants rich in glycosides and phenolics. Gentiana lutea L. is a highly sought-after plant for medicinal and industrial purposes because of its content of secondary metabolites. Not only G. lutea, but also Gentiana boissieri Schott et Kotschy ex Boiss., another Gentiana species distributed only in Turkey, is on the endangered species list and is not allowed to be collected in more than 100 countries. For this reason, the development of an efficient in vitro culture method to obtain the secondary metabolites synthesized in its roots is important, as it allows the production of higher amounts of these valuable metabolites. This study was conducted to determine the appropriate inoculum density (ID, fresh root weight per unit volume to be used), which is an important factor for biomass and secondary metabolite production, for root growth and production of phenolics and glycosides in the root culture of G. lutea and G. boissieri. For this purpose, roots were cultured at different IDs (5, 10, 15, 20, 25, and 30 g L−1) and evaluated for fresh root weight, root growth index, root dry weight, and content of iridoid (loganic acid), secoiridoids (swertiamarin, sweroside, gentinopicroside), xanthan (isogentisin), total phenolic, and some important phenolic compounds. All parameters were found to vary depending on the IDs. The result of the study was that the highest values in both root growth and production of most metabolites were obtained from the roots of G. lutea and G. boissieri cultivated with 20 g L−1 and 5 g L−1 IDs, respectively.
Aim : To investigate the analgesic effects of mangiferin (MAF) in experimental neuropathic pain (NPP) models and underlying mechanism. Methods : Databases including Pubmed, Web of Science, Embase, Cochrane Library, China national knowledge infrastructure (CNKI), Wangfang, and Weipu were used to search for the related studies. The search terms such as MAF, NPP, meta-analysis, rat, and mouse were used in various scientific databases. Meta-analysis was used to assay the analgesic efficacy of MAF. Further, molecular docking analysis was used to measure bindings of MAF and NPP-related proteins. Results : 6 studies were eventually included for the meta-analysis according to the inclusion and exclusion criteria. The meta-analysis results demonstrated that MAF significantly inhibited acetic acid-induced writhing responses, formalin-induced pain behavior, thermal analgesia, and mechanical allodynia as compared with the model control. However, risk of bias especially blinding existed in the included studies. The publication bias was checked by Begg's and Egger's tests. The molecular docking analysis indicated that MAF bound to 62 pain-related proteins at different levels. The top 10 predicted target proteins of MAF were glutamate carboxypeptidase II (GCPII), dopamine transporter (DAT), N-methyl-D-aspartic acid receptor 2B (NMDAR2B), transient receptor potential member 8 (TRPM8), monoamine oxidase type B (MAO-B), sodium voltage-gated channel alpha subunit 9A (SCN9A), fatty acid amide hydrolase (FAAH), gamma-secretase (GS), angiopoietin-1 (ANGPT1), and ANGPT2 based on the scores assessed by hydrogen bonding and hydrophobic interactions. Conclusions : In summary, MAF exerts the analgesic efficacy in the murine experimental pain models, which is associated with this compound binding to the NPP-related target proteins.
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Gentiana lutea is a wellknown and respected medicinal plant that is used in many pharmacopoeias, mainly against different gastrointestinal disorders. The plant is under protection regimes in its natural habitats and for that reason is grown on plantations. In addition, it could be cultivated in vitro . The plants grown on plantation and in in vitro conditions were used to prepare methanolic and 50% ethanolic extracts of root and leaf/shoot, which were tested for antigenotoxic and antibacterial properties, against foodborne mutagens (heterocyclic aromatic amines PhIP and IQ) and food contaminants, respectively. The results obtained pointed out the excellent genoprotective effect (up to 78% inhibition of PhIP/IQ genotoxicity) based mostly on the antioxidative potential. The antibacterial effect was mainly weak; only the extracts of in vitro grown plant induced moderate activity against Listeria monocytogenes and Staphylococcus aureus (MICs ranged 0.15-5 mg/ml). In addition, the extracts’ potential to prevent biofilm formation by L. monocytogenes was very high (up to 90% inhibition). Taken together, the results obtained encourage further research that would be directed to the formulation of potent antigenotoxic and antibiofilm agents based on G. lutea .
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Gentianella achalensis (Gilg) Ho & Liu extracts were tested for their activity against representative microorganisms cultures. The methanol (MeOH), aqueous and hydro-alcoholic extracts were active against Bacillus subtilis. From the bioguided fractionation of MeOH extract two active fractions were obtained (F1 and F7). The fractions were submitted to bioautography and HPLC/UV analysis and to further fractionation. The seco-iridoids gentiopicroside and sweroside and the xanthone demethylbellidifolin were identified as the active compounds from F1 and F7, respectively.
The antitubercular activity of four EtOH extracts prepared from leaves, flowers and roots of G. lutea, as well as of the isolated compound (isogentisin) was evaluated against Mycobacterium bovis. Extract D, obtained from flowers, showed strong inhibition with minimum inhibitory concentration (MIC) of 1000 μg/ml. As the extract D consisted of considerable proportion of isogentisin that compound was isolated and was subjected to antitubercular testing under identical experimental conditions. MIC for isogentisin was found to be 500 μg/ml.
Cited By (since 1996):6, Export Date: 18 October 2014
The chemical investigation of MeOH extracts of Gentiana lutea leaves and flowers showed that xanthones were one of the dominant class of compounds. Secoiridoids and flavonoids were also recorded. The amount of secondary metabolites varied depending on development stage. In the phase of flowering, leaves are rich with compounds possessing C-glycoside structures while O-glycoside structures accumulate mainly before flowering.
Gentiopicroside (1), a secoiridoid glycoside isolated from the methanol extract of the aerial parts of Centaurium erythraea, has been assessed for antibacterial and free radical scavenging activities. General toxicity of 1 has also been determined by brine shrimp lethality bioassay.
There is cumulative resistance against antibiotics of many bacteria. Therefore, the development of new antiseptics and antimicrobial agents for the treatment of skin infections is of increasing interest. We have screened six plant extracts and isolated compounds for antimicrobial effects on bacteria and yeasts with dermatological relevance. The following plant extracts have been tested: Gentiana lutea, Harpagophytum procumbens, Boswellia serrata (dry extracts), Usnea barbata, Rosmarinus officinalis and Salvia officinalis (supercritical carbon dioxide [CO2] extracts). Additionally, the following characteristic plant substances were tested: usnic acid, carnosol, carnosic acid, ursolic acid, oleanolic acid, harpagoside, boswellic acid and gentiopicroside. The extracts and compounds were tested against 29 aerobic and anaerobic bacteria and yeasts in the agar dilution test. U. barbata-extract and usnic acid were the most active compounds, especially in anaerobic bacteria. Usnea CO2-extract effectively inhibited the growth of several Gram-positive bacteria like Staphylococcus aureus (including methicillin-resistant strains - MRSA), Propionibacterium acnes and Corynebacterium species. Growth of the dimorphic yeast Malassezia furfur was also inhibited by Usnea-extract. Besides the Usnea-extract, Rosmarinus-, Salvia-, Boswellia- and Harpagophytum-extracts proved to be effective against a panel of bacteria. It is concluded that due to their antimicrobial effects some of the plant extracts may be used for the topical treatment of skin disorders like acne vulgaris and seborrhoic eczema.
Use of Gentiana lutea extracts as an antimicrobial agent
  • P Stierna
  • M Popp
Stierna P., Popp M., and Ismail C. (2005), Use of Gentiana lutea extracts as an antimicrobial agent. WO 2005025585 A1.