Chen H, Qian K, Tang ZP, Xing B, Chen H, Liu N et al.. Bioinformatics and microarray analysis of microRNA expression profiles of murine embryonic stem cells, neural stem cells induced from ESCs and isolated from E8.5 mouse neural tube. Neurol Res 32: 603-613

Department of Rehabilitation Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Neurological Research (Impact Factor: 1.44). 08/2009; 32(6):603-13. DOI: 10.1179/174313209X455691
Source: PubMed


To better understand whether microRNAs (miRNAs) are involved in the self-renewal of stem cells and fate determination of neural stem cells and to identify the miRNA expression patterns of different neural stem cells (NSC) in vitro and in vivo, we examined miRNA expression profiles of murine embryonic stem cells (ESC), NSC induced from ESC and isolated from E8.5 mouse neural tube (E8.5-NSC) using microarray technique. It was found that a total of 40 miRNAs had similar expression level in all the three cells [false discovery rate (FDR)=0, fold change <3.0]. Moreover, q-PCR showed that some members of miR-106b and miR-17-92 families were expressed in the ESC, NSC induced from ESC (ESC-NSC) and hematopoietic stem cells (HSC). Bioinformatical analysis showed that 'stemness genes' (p21/CDKN1A, p57/CDKN1C and PTEN) were putative targets of miR-106b and miR-17-92 families. A total of 95 miRNAs were found to experience significant change (FDR=0, fold change >5.0) when the ESC differentiated into NSC. On the basis of miRNA, mRNA expression variance and predicted target genes of miRNA, we formulated a bioinformatical model for miRNA control of ESC-NSC differentiation. Then, the miRNA expression pattern was compared between NSC obtained in vitro and in vivo, and it was found that only 8% of miRNAs were different between the two NSCs. This study suggested that miR-106b and miR-17-92 families may promote the renewal of stem cells by targeting PTEN, p21/CDKN1A and p57/CDKN1C. Some miRNAs may play a key role in gene re-programming during ESC-NSC differentiation, and a substantial homogeneity exists between NSCs derived in vitro and those in vivo.

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    • "Our results provided evidences that miR-17 and miR-93, another member of miR-17 family can also repress BMPRII translation in primary neurons. This regulation is important since it is reported that miR-17 family is highly expressed in the early development of nervous system [34]. The high level of miR-17 family may target BMPRII leading to the down-regulation of BMP signaling, while the repression of BMP signaling has been prove to facilitate neurogenesis [8]. "
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    Full-text · Article · Dec 2013 · PLoS ONE
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    • "The miRNA miR-17-92 cluster was first identified in the year 2005 as a potential human oncogene by He et al., and it was demonstrated that tumors derived from hematopoietic stem cells expressed a subset of the mir-17-92 cluster (He et al., 2005). miR-17-92 members have been identified as being overexpressed in numerous types of stem cells, such as hematopoietic stem cells (Jin et al., 2008), embryonal carcinoma SCs (Gallagher et al., 2009), mouse embryonic SCs (Gunaratne, 2009) and neuronal SCs (Chen et al., 2010), and are downregulated during neuronal lineage differentiation in unrestricted somatic stem cells (Iwaniuk et al., 2011) and in unrestricted somatic stem cells differentiated Fig. 7. Kaplan–Meier survival curves of gastric cancer patients expressing miR-20a or miR-92a. (A,B) Gastric cancer patients were ranked on the basis of the relative expression values obtained by real-time PCR and were divided into a high-expression group and low-expression group using the 50th percentile (median) as the cut-off point. "
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