IADR 90th General Session.

Abstract

The search for elaborate tridimensional scaffolds materials with high mechanical stability, which can adapt to the surgical site, allow oseointegration and degrade rapidly, are more often needed factors for the regeneration of bone tissue. Objective: Analyze the osteoblastic growth capability over different materials to determine which of all is the best for the fabrication of tridimensional scaffolds which allow bone regeneration. Method: Tridimensional structures were fabricated with porous silice, hydroxilapatite and collagen. Bovine bone was taken as material control. Afterwords the toxicity of the material was determined by planting primary osteoblasts obtains from articulate replacements over this tridimensional structures. Cellular morphology was assessed using S.E.M. and cellular viability through confocal microscopy using the DEAD/LIVE kit cell Viability from INVITROGEN ®. Result: The colonization of the osteoblasts was observed on the surface of the materials. These osteoblasts presented different forms of morphology going from round cells to flat cells with extensions (philopodyas) of different sizes forming various caps. The cellular viability was positive for all materials which indicate that none of the material used produced citotoxicity to the osteoblasts. Conclusion: This study shows the osteoblastic growth and its viability in 4 different materials with different composition and characteristics which suggest that these materials could be used in the future as scaffolds with bone regeneration purposes in dental and medical areas. Keywords: Bone repair, Osteoblasts/osteoclasts, Regeneration, Structure and Tissue engineering