Conference Paper

Preliminary characterization of bacteria isolated from hot springs in Maha Oya, Sri Lanka.

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... Serial dilution technique and colony count method were used in the quantitative analysis of bacteria at various temperatures (30°C -70°C) and pH (6,7,8) [11]. ...
... Serial dilution technique and colony count method were used to estimate the number of colonies (30 300) presented in the test sample. Table 2 shows the Log CFU/ml (colony forming units per ml) present in 30°C -80°C of Laceyella sacchari and Thermus aquaticus [11]. ...
... According to the results (Table 2) and comparison with literature [11]. According to the results ( Table 2) and comparison with literature [11] all isolates showed very good to excellent growth between 40°C -60°C but Laceyella sacchari growth were inhibited at 70°C. ...
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Thermophilc bacteria have a greater advantage in biotechnological application due to their production of enzymes such as proteases with high thermostability enzymes. The aim of the present study was the optimization of the growth conditions on protease production by Laceyella sacchari, and Thermus aquaticus isolated from Maha Oya Geothermal springs in Sri Lanka. The selected thermophilic bacteria belonging to the genera of Laceyella and Thermus which were previously being isolated and identified were used in the present investigation to study their protease production. Among the two isolates, Laceyella sacchari (DMBUK 14901) reached a maximum protein concentration with a level of 490 μg/ml and showed maximum protease activity of 1.7831 units/ml at 55°C, at pH 7. According to the final result observation of temperature and pH on protease activity discovered that the extreme protease activity was detected at 60°C over a pH range of 6. The isolates of Laceyella and Thermus consumed several carbon and nitrogen sources for the synthesis of proteases. Both Laceyella and Thermus showed maximum protease activity in the presence of sucrose as a carbon sources. Furthermore, isolates of Laceyella and Thermus showed a maximum protease activity when the gelatin was used as the nitrogen source.
... Serial dilution technique and colony count method were used in the quantitative analysis of bacteria at various temperatures (30°C-70°C) and pH (6,7,8) respectively [9]. ...
... According to results, almost all of these cultures were showed excellent growth at pH 6, 7 and 8 and the results are given in the [9,16]. So pH 7 was showing good growth of isolates. ...
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