In Vitro Propagation of Citrus Rootstocks

Notulae Botanicae Horti Agrobotanici Cluj-Napoca (Impact Factor: 0.55). 01/2009; 37(1).
Source: DOAJ


Present investigation was conducted to standardize a protocol for in-vitro propagation of citrus rootstocks viz. Rough lemon, Cleopatra mandarin Pectinifera and Troyer citrange. The shoot tip explant was found better for callus induction of these rootstocks than the nodal segment. Maximum callus formation (40.0% and 23.3%) of shoot tip explants was obtained in Cleopatra mandarin, Pectinifera, and Rough lemon and Troyer citrange, respectively in treatment MS basal media + 0.5mg/l Kin, 2.0mg/l NAA, and 2.0mg/l 2, 4-D. Furthermore, the maximum number of shoots per explant was obtained through the callus in Pectinifera, Rough lemon and Cleopatra mandarin in MS basal media + BAP 1mg/l. Maximum rooting of shoots (1.11%) was noted in rootstock Rough lemon followed by Cleopatra mandarin for the ½ MS media supplemented with 10mg/l IBA. Although the callus development and bud proliferation was recorded in rootstock Troyer citrange however, shoot and root formation did not occur. The potting media consisting of soil, sand and FYM in the ratio of 1:1:1 by volume was better with maximum survival rate of hardened plants six weeks after transferring to the pots under greenhouse for Rough lemon followed by Pectinifera and Cleopatra mandarin rootstock.

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    • "Earlier also culture of callus of different spp. of Citrus on MS medium supplemented with different concentrations of BA has shown good regeneration response (Pena et al. 1995a, b; Normah et al. 1997; Cervera et al. 1998; Chakraborty and Goswami 1999; Pena and Navarro 1999; Costa et al. 2002). Similarly, Sharma et al. (2009) reported best shoot regeneration response from shoot tip callus cultured on MS medium supplemented with BA (1 mg/L). Absence of 2,4-D and presence of BA and KN in medium initiates shoot induction from the callus as reported earlier in other species too (Raman et al. 1992). "
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    ABSTRACT: Citrus jambhiri Lush. (family Rutaceae), commonly known as 'rough lemon', is one of the favourite rootstocks for lemons, oranges, mandarins, grape fruits and kinnows in Punjab. The present investigation deals with development of an efficient miropropagation protocol for Citrus jambhiri Lush. using cotyledons as explant. Maximum callus induction (91.66 %) was observed on MS medium supplemented with 2,4-D (2 mg/L) in combination with ME (500 mg/L). Green healthy calli were cut into small pieces and cultured on MS medium for regeneration. Maximum shoot regeneration (87.50 %) was observed with BA (3 mg/L). Effect of increasing age of callus was also studied which showed that callus retained regeneration capacity (58.33 %) even after 420 days of culture. Regenerated shoots were separated out and cultured on rooting medium. Maximum rooting response (91.67 %) was observed on half strength MS medium supplemented with NAA (0.5 mg/L). After hardening and acclimatization the plantlet were transferred to the field and showed 67 % survival.
    Full-text · Article · Apr 2011 · Physiology and Molecular Biology of Plants
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    • "Tissue culture and micropropagation protocols have been described for a number of Citrus species and explant sources (Barlass and Skene, 1982; Duran-Vila et al., 1989; Raman et al., 1992; Normah et al., 1997; Chakravarty and Goswami, 1999; Al-Khayri and Al-Bahrany, 2001; Filho et al., 2001; Usman et al., 2005; Altaf et al., 2009a,b; Khan et al., 2009; Laskar et al., 2009; Sharma et al., 2009; Pe´rez- Tornero et al., 2010). However, little work has been carried out on the tissue culture aspect of C. jambhiri (Altaf and Ahmad, 1997; Ali and Mirza, 2006; Altaf et al., 2008). "
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    ABSTRACT: Citrus jambhiri (rough lemon) is an important rootstock for a number of Citrus fruit crops including lemons, oranges, grape fruits, kinnows and mandarins. The present study deals with establishment of protocol for micropropagation of C. jambhiri via callus induction and regeneration. Leaf segments, nodal segments and root segments excised from in vitro raised seedlings were used as explants. Maximum callus induction (98.66%) was observed from leaf segments on MS medium supplemented with 2, 4-D (4 mg/L). For nodal segments, maximum callus induction (96%) was observed with 2, 4-D (1 mg/L) and from root segments, it was 48.66% on MS medium supplemented with 2, 4-D (2 mg/L). Callus raised from leaf segments showed maximum regeneration (57%) with NAA (0.5 mg/L) + BA (1 mg/L) whereas nodal segments showed better regeneration (71.89%) with NAA (0.5 mg/L) + BA (3 mg/L). However, callus from root segments did not regenerate. Regenerated shoots were rooted on MS medium supplemented with different plant growth regulators and best response (71%) was observed with NAA (0.5 mg/L).
    Full-text · Article · Jan 2010
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    ABSTRACT: In citrus, production of mature transgenic plants belonging to different genotypes is an important biotechnological objective. In the present study, we tried to genetically transform and regenerate mature plants from the economically important Navelina sweet orange cultivar by using the procedure previously established for the genetically close Pineapple sweet orange variety. The use of BAP at 3mgl−1 promoted efficient shoot organogenesis in Pineapple as expected, but not in Navelina. Furthermore, different effects were observed when the auxin α-naphtalene acetic acid (NAA) was added to BAP-containing regeneration media. Although NAA addition at 0.5mgl−1 enhanced cambial callus formation, number of shoots and their elongation in Navelina, the contrary effect was observed in Pineapple. Moreover, transformation efficiency in Navelina rose from 0 to 3% but declined from 6 to 0% in Pineapple, indicating that BAP and BAP+NAA exerted the opposite effect in transgenic shoot regeneration from two closely related cultivars. This suggests that small changes in the procedure could induce drastic alterations in regeneration and even increase the likelihood of obtaining transformants from non-responsive genotypes. Moreover, the vigour of the starting plant material and the addition of kanamycin as selective agent were determining for the generation of mature sweet orange transgenic plants.
    No preview · Article · Apr 2008 · Plant Cell Tissue and Organ Culture
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