It is believed that music has the power to soften emotions and alleviate pains. The essence of the power has being encoded by researchers. This study was aimed to explore the effects of music preference and stress-associated responses of college students when they listen to music. The objectives of this study were (1) to survey the music types of relaxing music for college students, and the difference in gender and study majors; (2) to investigate the effects of musical preference, music expertise, and awareness of musical content on their perceptivity of relaxation; and (3) to analyze the relativities of musical emotions with musical characteristics, such as tempo, mode, and dynamic range. Participants were asked to listen to selected music pieces, and to rate their three-dimensional emotional responses, pleasant-unpleasant, calm-arousal, and relaxing-stress, on five-point Likert scales. Data collection of music compositions and personal music taste was acquired using surveys. The findings are expected to (1) understand college students' listening habit, (2) collect repertoire suitable for university students in terms of stress releasing, and (3) offer advices for music appreciation teaching, psychological consultation personnel, and clinical therapist.