Some Biological Activities and Safety of Mineral Pitch

Article · January 2008with97 Reads
DOI: 10.14456/sustj.2008.6 · Source: DOAJ
Abstract
Mineral pitch, a form of mineral dripping from the cracks of the rocks, has been historically used through topical and oral administrations for health benefits. Its biological activities and safety have not been well characterized. The purpose of this study was to investigate the antioxidative activity, antimicrobial activity, cytotoxicity and heavy metal determination of mineral pitch in comparison to coal tar. The total phenolic content and antioxidative activity of mineral pitch were higher than those of coal tar. Antimicrobial activities against Staphylococcus aureus , Escherichia coli and Candida albicans of mineral pitch were less than coal tar. A dichloromethane extract of mineral pitch could inhibit the growth of those three microbes, while the butanol extract showed the growth inhibition on S. aureus and C. albicans . From the MTT assay, mineral pitch was notably toxic to normal human lung fibroblast (MRC-5), human breast carcinoma cells (MDA-MB-231), human lung carcinoma cells (A549), human cervical carcinoma cells (Hela), human colorectal adenocarcinoma cells (SW-620), human ovarian carcinoma cells (SKOV-3) and human hepatocarcinama cells (HepG2). Inductively coupled plasma-mass spectrometry (ICP-MS) analysis indicated the high content of heavy metals, especially, As, Hg, and Pb in mineral pitch which might relate to the cytotoxicity.
    • Laparotomy and pylorus ligation were performed under ether anesthesia. The pylorus of the stomach and esophagocardiac junction were immediately ligated, and the stomach was then removed from each rat, and the gastric contents were collected, centrifuged, and the supernatants were used for pH measurement using pH meter (Ø 50 pH Meter, Beckman, Fullerton, CA, USA).[17] Ethical considerations
    [Show abstract] [Hide abstract] ABSTRACT: Gastric ulcer is an important clinical problem, chiefly due to extensive use of some drugs. The aim was to assess the activity of Mumijo extract (which is used in traditional medicine) against acetic acid induced gastric ulcer in rats. The aqueous extract of Mumijo was prepared. Animals were randomly (n = 10) divided into four groups: Control, sham-operated group (received 0.2 ml of acetic acid to induce gastric ulcer), Mumijo (100 mg/kg/daily) were given for 4 days postacetic acid administration, and ranitidine group (20 mg/kg). The assessed parameters were pH and pepsin levels (by Anson method) of gastric contents and gastric histopathology. Ranitidine was used as reference anti-ulcer drug. The extract (100 mg/kg/daily, p.o.) inhibited acid acetic-induced gastric ulceration by elevating its pH versus sham group (P < 0.01) and decreasing the pepsin levels compared to standard drug, ranitidine (P < 0.05). The histopathology data showed that the treatment with Mumijo extract had a significant protection against all mucosal damages. Mumijo extract has potent antiulcer activity. Its anti-ulcer property probably acts via a reduction in gastric acid secretion and pepsin levels. The obtained results support the use of this herbal material in folk medicine.
    Full-text · Article · Feb 2015
  • [Show abstract] [Hide abstract] ABSTRACT: Objective(s): Brain edema is one of the most serious causes of death within the first few days after trauma brain injury (TBI). In this study we have investigated the role of Shilajit on brain edema, blood-brain barrier (BBB) permeability, intracranial pressure (ICP) and neurologic outcomes following brain trauma. Materials and Methods: Diffuse traumatic brain trauma was induced in rats by drop of a 250 g weight from a 2 m high (Marmarou's methods). Animals were randomly divided into 5 groups including sham, TBI, TBI-vehicle, TBI-Shi150 group and TBI-Shi250 group. Rats were undergone intraperitoneal injection of Shilajit and vehicle at 1, 24, 48 and 72 hr after trauma. Brain water content, BBB permeability, ICP and neurologic outcomes were finally measured. Results: Brain water and Evans blue dye contents showed significant decrease in Shilajit-treated groups compared to the TBI-vehicle and TBI groups. Intracranial pressure at 24, 48 and 72 hr after trauma had significant reduction in Shilajit-treated groups as compared to TBI-vehicle and TBI groups (P<0.001). The rate of neurologic outcomes improvement at 4, 24, 48 and 72 hr after trauma showed significant increase in Shilajit-treated groups in comparison to theTBI-vehicle and TBI groups (P <0.001). Conclusion: The present results indicated that Shilajit may cause in improvement of neurologic outcomes through decreasing brain edema, disrupting of BBB, and ICP after the TBI.
    Full-text · Article · Jan 2013
  • [Show abstract] [Hide abstract] ABSTRACT: Objective(s): Brain edema is one of the most serious causes of death within the first few days after trauma brain injury (TBI). In this study we have investigated the role of Shilajit on brain edema, blood-brain barrier (BBB) permeability, intracranial pressure (ICP) and neurologic outcomes following brain trauma. Materials and Methods: Diffuse traumatic brain trauma was induced in rats by drop of a 250 g weight from a 2 m high (Marmarou’s methods). Animals were randomly divided into 5 groups including sham, TBI, TBI-vehicle, TBI-Shi150 group and TBI-Shi250 group. Rats were undergone intraperitoneal injection of Shilajit and vehicle at 1, 24, 48 and 72 hr after trauma. Brain water content, BBB permeability, ICP and neurologic outcomes were finally measured. Results: Brain water and Evans blue dye contents showed significant decrease in Shilajit-treated groups compared to the TBI-vehicle and TBI groups. Intracranial pressure at 24, 48 and 72 hr after trauma had significant reduction in Shilajit-treated groups as compared to TBI-vehicle and TBI groups (P<0.001). The rate of neurologic outcomes improvement at 4, 24, 48 and 72 hr after trauma showed significant increase in Shilajit-treated groups in comparison to theTBI- vehicle and TBI groups (P <0.001). Conclusion: The present results indicated that Shilajit may cause in improvement of neurologic outcomes through decreasing brain edema, disrupting of BBB, and ICP after the TBI.
    Full-text · Article · Jul 2013