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A Novel Fusarium Species Causes a Canker Disease of the Critically Endangered Conifer, Torreya taxifolia


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A canker disease of Florida torreya (Torreya taxifolia) has been implicated in the decline of this critically endangered species in its native ranee of northern Florida and southeastern Georgia. In surveys of eight Florida torreya sites, cankers were present on all dead trees and 71 to 100% of living trees, suggesting that a fungal pathogen might be the causal agent. To identify the causal agent, nuclear ribosomal internal transcribed spacer region (ITS rDNA) sequences were determined for 115 fungi isolated from cankers on 46 symptomatic trees sampled at three sites in northern Florida. BLASTn searches of the GenBank nucleotide database, using the ITS rDNA sequences as the query, indicated that a novel Fusarium species designated Fsp-1 might be the etiological agent. Molecular phylogenetic analyses of partial translation elongation factor I-alpha (EF-1) and RNA polymerase second largest subunit (RPB2) gene sequences indicate that Fsp-1 represents a novel species representing one of the earliest divergences within the Gibberella clade of Fusarium. Results of pathogenicity experiments established that the four isolates of Fsp-1 tested could induce canker symptoms on cultivated Florida torreya in a growth chamber. Koch's postulates were completed by the recovery and identification of Fsp-1 from cankers of the inoculated plants.
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Plant Disease / June 2011 633
A Novel Fusarium Species Causes a Canker Disease of the Critically
Endangered Conifer, Torreya taxifolia
Jason A. Smith, School of Forest Resources and Conservation, University of Florida, Gainesville; Kerry O’Donnell, Bacterial Food-
borne Pathogens and Mycology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL; Lacey L.
Mount, Department of Plant Pathology, University of Florida, Gainesville; Keumchul Shin, Kelly Peacock, and Aaron Trulock,
School of Forest Resources and Conservation, University of Florida, Gainesville; Tova Spector, Florida Park Service, Department of
Environmental Protection, Panama City; and Jenny Cruse-Sanders and Ron Determann, Atlanta Botanical Garden, Atlanta, GA
Smith, J. A., O’Donnell, K., Mount, L. L., Shin, K., Peacock, K., Trulock, A., Spector, T., Cruse-Sanders, J., and Determann, R. 2011. A novel
Fusarium species causes a canker disease of the critically endangered conifer, Torreya taxifolia. Plant Dis. 95:633-639.
A canker disease of Florida torreya (Torreya taxifolia) has been impli-
cated in the decline of this critically endangered species in its native
range of northern Florida and southeastern Georgia. In surveys of eight
Florida torreya sites, cankers were present on all dead trees and 71 to
100% of living trees, suggesting that a fungal pathogen might be the
causal agent. To identify the causal agent, nuclear ribosomal internal
transcribed spacer region (ITS rDNA) sequences were determined for
115 fungi isolated from cankers on 46 symptomatic trees sampled at
three sites in northern Florida. BLASTn searches of the GenBank nu-
cleotide database, using the ITS rDNA sequences as the query, indi-
cated that a novel Fusarium species designated Fsp-1 might be the
etiological agent. Molecular phylogenetic analyses of partial transla-
tion elongation factor 1-alpha (EF-1) and RNA polymerase second
largest subunit (RPB2) gene sequences indicate that Fsp-1 represents a
novel species representing one of the earliest divergences within the
Gibberella clade of Fusarium. Results of pathogenicity experiments
established that the four isolates of Fsp-1 tested could induce canker
symptoms on cultivated Florida torreya in a growth chamber. Koch’s
postulates were completed by the recovery and identification of Fsp-1
from cankers of the inoculated plants.
Canker disease of Florida torreya, known as gopherwood or
stinking cedar (Torreya taxifolia Arn.), hereafter referred to as
CDFT, appears to have contributed to this plant being listed as
critically endangered by the U.S. Fish and Wildlife Service. Cur-
rently, this endemic taxaceous conifer is restricted to bluffs and
ravines along the Apalachicola River in Gadsden and Liberty coun-
ties in Florida and Decatur County in Georgia (16). T. taxifolia is
considered the rarest conifer in North America and one of the most
endangered species in the world (4).
Although the decline of Florida torreya was first observed in the
late 1930s (1), the tree was still common in its habitat in northern
Florida and southeastern Georgia through the 1950s. The rapid
decline of the species in the early 1900s was initially attributed to
an unknown fungal disease based on the abundance of leaf spots
and stem cankers (7). Due to decline, and to the lack of seed-bear-
ing trees (1), Florida torreya was considered to be destined for
extinction (7). In addition to the decline, this species has been
negatively impacted by changes in hydrology, forest structure,
heavy deer browse, and a loss of reproductive capability (17). Flor-
ida torreya stems killed by disease often re-sprout in a manner
reminiscent of American chestnut following Chestnut Blight. Flor-
ida torreya has declined by more than 99% over the past century
from an estimated population of 357,500 individuals in 1914 to
approximately 1,350 in the 1990s (19,20), to current estimates of
400 to 600 individuals (T. Spector, personal communication). Trees
in their native range have not reproduced from seed for several
decades (18). Despite several attempts to conclusively determine
the causal agent of Florida torreya decline, disease etiology has not
been elucidated (1–3,8,22). In the first pathology studies conducted
on T. taxifolia (1), it was noted that leaf spots, needle necrosis,
defoliation, and stem lesions were common on native and culti-
vated T. taxifolia. Several pathogens were commonly isolated from
symptomatic needles (Macrophoma sp., Rhizoctonia solani,
Sphaeropsis sp., and Sclerotium rolfsii); however, no pathogens
were isolated from cankered stems and Koch’s postulates were not
completed. Subsequently, El-Gholl (3) reported Fusarium laterit-
ium as a causal agent by demonstrating this species’ capacity to
cause leaf spots. Alfieri et al. (2) completed Koch’s postulates with
F. lateritium as a leaf spot pathogen, but did not address whether
this species could induce the canker disease. While Schwartz et al.
(22) implicated Pestalotiopsis microspora as the causal agent of the
canker disease, no information was given on canker development,
morphology, or ability to cause mortality. Artificial inoculations
using P. m ic ro sp or a resulted in stem canker development (8), but
stem mortality was not observed. These reports are considered to
be inconclusive given that Pestalotiopsis spp. are considered to be
weak opportunistic pathogens (23). Subsequent studies implicated
a Scytalidium sp. due to frequent isolation from cultivated and
naturally occurring Florida torreya. Artificial inoculations resulted
in small lesions on needles, but cankers were not observed.
In addition to biotic causes of decline, several studies have re-
ported on changes in soils, drought, global warming, sunlight
exposure, and fire regime as possible causes of decline (21). Some
of these environmental changes are thought to have occurred be-
cause of the building of the Woodruff Dam along the Apalachicola
River in 1957, and changing land uses in the surrounding areas.
However, none of these environmental hypotheses has been
demonstrated as a cause of the decline.
Current efforts to manage this endangered species have been
hindered by a lack of understanding of the current and historic
causes of disease of Florida torreya. As a result, various agencies
have taken different approaches to manage Florida torreya depend-
ing on which cause the decline is attributed to. For these reasons,
more information is needed about the etiology of CDFT in order to
develop sound management practices. Accordingly, the present
study was conducted to: (i) assess canker incidence among natural
populations of T. taxifolia, and (ii) identify the causal agent of
Corresponding author: Jason A. Smith, E-mail:
Accepted for publication 26 January 2011.
doi:10.1094 / PDIS-10-10-0703
© 2011 The American Phytopathological Society
634 Plant Disease / Vol. 95 No. 6
Materials and Methods
Field surveys. During 2008 to 2010, surveys were conducted for
all living T. taxifolia on public and private lands in Gadsden and
Liberty counties in Florida where this species had been reported
previously. Surveys were conducted using historical maps/data,
personal knowledge of the sites, and visual searching. Once lo-
cated, individual trees were measured for height and diameter at
ground line (DGL). Due to stem heights commonly <1 m, diameter
at breast height could not be measured for most specimens. The
condition of each tree and occurrence of stem cankers was re-
corded. Position on slope, soil conditions, associated flora, occur-
rence of leaf spots and canopy cover/light intensity were also re-
corded. For canker incidence, site means were analyzed using one-
way analysis of variance (ANOVA) and Duncan’s multiple range
test (P < 0.05) (SAS ver. 9.1).
Isolation and tentative identification of fungi. Stem tissue
from the margins of 150 cankers were collected in the field from
46 diseased trees collected from three locations and returned to the
laboratory. Samples were cut to approximately 10 mm2 and surface
sterilized in 5% sodium hypochlorite for 30 s, followed by a 10-s
rinse in sterile H2O. The samples were placed on 2% potato dex-
trose agar (PDA; DIFCO, Detroit, MI) and incubated at room tem-
perature for 5 to 7 days. Fungal colonies were then subcultured on
PDA for subsequent identification and inoculation experiments.
A total of 129 isolates were used for identification based on
ribosomal internal transcribed spacer region (ITS-rDNA) se-
quences. DNA was extracted using the Qiagen DNeasy Plant Mini
kit (Qiagen Inc., Valencia, CA) following the manufacturer’s in-
structions except that samples were ground using liquid nitrogen
and a mortar and pestle prior to extraction, and the 65°C incubation
step was increased to 1 h. PCRs using the universal primers ITS1
and ITS4 (26) were performed with the following reaction mixture:
1 µl of diluted (1:100) template DNA, 1 µl of each primer (10 µM),
9.5 µl of ddH2O, and 12.5 µl of Amplitaq Gold Master Mix (Ap-
plied Biosystems, Emeryville, CA). PCRs were performed in a MJ
Mini thermocycler (BioRad Inc., Hercules, CA) with the following
thermocycling profile: 94°C for 5 min followed by 35 cycles of
94°C for 1 min, 55°C for 1 min, 72°C for 1 min, followed by a
final extension step at 72°C for 5 min. PCR amplicons were visual-
ized on a 1.5% agarose gel and were purified prior to sequencing
using the EXOSAPit kit (USB Corp., Cleveland, OH). Amplicons
were sequenced at the University of Florida Interdisciplinary Cen-
ter for Biotechnology Research using an ABI3730 DNA Analyzer
(Applied Biosystems Inc., Foster City, CA). Forward and reverse
sequences were edited and contigs were aligned using ChromasPro
ver. 1.5 software (Technelysium Inc., Tewantin QLD, Australia).
Edited sequences were used for BLASTn searches of the GenBank
nucleotide database ( Fungi were
identified based on top BLAST results (lowest e-value, highest
score, and greatest similarity).
Inoculation experiments. Seven of the most frequently isolated
fungi (Botryosphaeria obtusa [Bo-Tt1], Fusarium cf. lateritium
[Fl-Tt1], F. solani species complex [Fs-Tt1], Fusarium sp. 1 [Fsp-
1], Fusarium sp. 2 [Fsp-2], Lasiodiplodia theobromae, and Pes-
talotiopsis sp.) were used in inoculation experiments with potted,
seed-grown T. taxifolia provided by the Atlanta Botanical Garden.
For all three inoculation experiments (Table 1), inoculation points
were made on each plant by making a wound using a sterile single-
edge blade to make a vertical slit under the bark approximately 10
× 5 mm in size. The inoculum, a mycelium plug approximately 5
mm2, was inserted into the wound, which was wrapped in Parafilm.
For mock-inoculated plants, wounds were made in the same man-
ner, but sterile PDA plugs were used instead of mycelium plugs.
The plants were maintained in a growth chamber with 16-h day
length and temperatures of 25°C (light) and 18°C (dark) for 6
months following inoculation. For inoculation experiment 1 (IE1),
one isolate of all species were used to inoculate the stems of one
plant each of 2-year-old seedlings (average height approximately
25 cm). One mock-inoculation was done on a separate plant as a
control (Table 1). Each inoculation point was made on each plant
at approximately 5 cm above the ground line. The Parafilm was
removed after 2 weeks, and plants were monitored for symptom
development at weekly intervals. Cankers were measured 3 months
following inoculation, and percent stem circumference cankered
was determined and mortality was measured after 6 months.
In inoculation experiment 2 (IE2), three different isolates of Fsp-1
were used (NRRL 54152 = 542, NRRL 54154 = 587, and NRRL
54155 = 596) to verify results obtained in IE1. Despite causing a
canker in experiment IE1, FSSC isolate Fs-Tt1 was not used in
subsequent experiments because it was infrequently isolated from
cankers in the field (<1%) and was not considered a likely candi-
date causal agent. All of the methods were the same, except a total
of six plants were inoculated with two replicates per isolate. One
mock-inoculation on a separate plant was included as a control
(Table 1).
Inoculation experiment 3 (IE3) was performed using plants of
two different sizes: 2-year-old seedlings as used before (average
height = 27 cm; average stem diameter = 0.6 cm), and larger sap-
lings, approximately 5 years old (average height of 89 cm and
average stem diameter of 1.2 cm). The larger saplings approximate
the size of the trees re-sprouting and displaying canker symptoms
in the field. Seven different isolates of Fsp-1 were used to inoculate
plants (Torreya isolates listed in Table 2). Each isolate was inocu-
lated onto one plant of the smaller size and two plants each of the
larger size with three inoculation points on each plant for a total of
nine inoculations per isolate. Two plants, one of each size, were
mock inoculated (three inoculations per plant as described above)
and served as controls (Table 1). Inoculation points on the smaller
plants were distanced approximately 3 cm apart on the stems start-
ing at 2.5 cm above ground line (and spiraling up the stem) and 5
cm apart on the larger plants starting at 10 cm above ground line.
In addition to percent stem circumference girdled for each canker,
mortality was assessed after 6 months.
Molecular phylogenetics. Isolates of Fsp-1 were cultured in
yeast-malt broth for 2 to 3 days at 24°C, after which total genomic
DNA was isolated from freeze-dried mycelium using a hexadecyl-
trimethyl-ammonium bromide (Sigma-Aldrich, St. Louis, MO)
protocol published previously (10). Seven isolates of the novel
Fusarium sp.-1 (Fsp-1) of T. taxifolia (NRRL 54149-54155; Table
2) were characterized genetically by analyzing DNA sequences of
the nuclear ribosomal internal transcribed spacer region (ITS
rDNA), and partial sequences of translation elongation factor (EF-
) and RNA polymerase second largest subunit (RPB2). The ITS
rDNA region was PCR amplified and sequenced with primers
was PCR amplified with primers
ACCAGTSATCATG and sequenced with EF-3> GTAAGG
CGAGCTC; and RPB2 was PCR amplified and sequenced as two
contiguous fragments using primers 5f2> GGGGWGAYCAGA
Tab le 1 . Numbers of seedlings and treatments in inoculation experiments
Experiment no.
Total plants No. inoculated
with Fsp-1 No. Fsp-1
isolates used No. inoculated
with other fungi No. mock-
inoculated Inoculations
per plant
IE1 8 1 1 6 1 1
IE2 7 6 3 0 1 2
IE3 16 14 7 0 2 3
Plant Disease / June 2011 635
TCRTCSACC as previously described (10,11,14,26). Platinum Taq
DNA polymerase (Invitrogen Life Technologies, Carlsbad, CA)
was used in all PCR reactions. Following PCR amplification, am-
plicons were sized by gel electrophoresis in 1.5% agarose gels
(Invitrogen) run in 1× TAE buffer (15), after which they were
stained with ethidium bromide and visualized over a UV trans-
illuminator. Prior to sequencing with Applied Biosystems BigDye
version 3.1 Terminator reaction mix (ABI, Emeryville, CA), ampli-
cons were purified using Montage96 filter plates (Millipore Corp.,
Billerica, MA). Sequencing reaction mixes were conducted in a
10-µl volume and contained 2 µl of ABI BigDye version 3.1-termi-
nator reaction mix, 2 to 4 pmol of a sequencing primer, and ap-
proximately 50 ng of amplicon (10). Sequencing reaction mixes
were purified using ABI XTerminator and then run on an ABI 3730
automated sequencer. Sequence chromatograms were edited for
accuracy and aligned with Sequencher version 4.9 (Gene Codes,
Ann Arbor, MI) prior to being exported as NEXUS files for subse-
quent analyses.
To assess the genetic diversity of the Florida torreya pathogen,
we conducted phylogenetic analyses of the aligned partial EF-1
and RPB2 gene sequences employing maximum parsimony in
PAUP (ver. 4.0b10; Sinauer Associates, Inc., Sunderland, MA; 24)
and maximum likelihood in GARLI (ver. 0.951; 27). Maximum
parsimony (MP) analyses were conducted in PAUP using the
branch-and-bound option for an exact solution. The general-time-
reversible model with a proportion of invariant sites and gamma
distributed rate heterogeneity was employed as the model of nu-
cleotide substitution when using maximum likelihood (ML) as the
optimality criterion. To investigate evolutionary relationships,
RPB2 sequences were obtained for five Fsp-1 isolates and 19 addi-
tional phylogenetically diverse fusaria. Over half of the latter were
represented by fusaria reported to be tree pathogens (6,9; Table 2).
The best ML tree, based on 10 independent analyses of the RPB2
dataset, yielded a log-likelihood of –9930.87 (Fig. 1). Clade sup-
port was assessed by 1,000 MP and ML bootstrap pseudoreplicates
of the data (24,27). DNA sequences generated in this study have
been deposited in GenBank under accession numbers HM068337–
Field surveys. A total of 225 trees were located on eight sites
with an average of 28 trees and a range of 15 to 47 trees per site.
Average height and diameter at ground line among all eight sites
were 118.5 cm and 1.9 cm, respectively. Average height ranged
from 77.7 to 262.6 cm, and average DGL ranged from 0.87 to 4.4
cm. Canker incidence ranged from 71 to 100% depending on site
(Figs. 1 and 2) with an average incidence of 93.4%. However,
means for canker incidence were only statistically significant be-
tween the TNC Sweetwater Tract and all other sites (at the P <
0.05 level; see Fig. 2).
Isolation and tentative identification of fungi. Samples were
collected from cankers from a total of 46 (20% of the currently
known and surveyed population) trees from three sites (Torreya
State Park, TNC-Sweetwater Tract, and TNC-Aspalaga Tract)
representing a total of 150 samples. From these samples, nuclear
ribosomal ITS rDNA sequences were obtained for 115 isolates.
These sequences were used to query the GenBank database to
identify the isolates. The most frequently isolated species appeared
to represent a novel unidentified Fusarium species (28.7% of all
isolates and 44.0% of all sampled trees), designated Fsp-1, based
on the results of the BLASTn searches (e-values > 0.0). Unidenti-
fied fungi constituted the next most common group (21.7% of all
isolates and 40.3% of all sampled trees), followed by saprophytes
(22.5% of all isolates and 50.0% of all sampled trees) and Pestalo-
tiopsis spp. (10.8% of all isolates and 30.4% of all sampled trees).
The remaining isolates were identified as known or potential
pathogens (B. obtusa, F. cf. lateritium, members of the FSSC [12],
unidentified Fusarium sp-2 [Fsp-2], L. theobromae) but constituted
a low percentage (3.9% of all isolates and only 10.9% of all sam-
pled trees).
For the Torreya State Park samples, cankers from 31 trees were
sampled, resulting in 91 isolates, with 78 being successfully se-
Tab le 2 . Isolates used in the phylogenetic analysis
NRRL no.a Equivalent no.b Fusarium speciesc Host/substrate Geographic origin
13622 FRC L-55 Fusarium cf. lateritium Ulmus americana LA-USA
20956 CBS 123670 Fusarium verticillioides Zea mays CA-USA
22161 ATCC 18692 FSSC 13-a (Fusarium solani f. sp. robiniae) Robinia pseudoacacia Japan
22163 ATCC 18690 FSSC 22-a (Fusarium solani f. sp. xanthoxyli) Xanthoxylum piperitum Japan
22230 ATCC 44934 FSSC 17-b (Fusarium solani f. sp. mori) Morus alba Japan
22316 ATCC 66906 Fusarium staphyleae Staphylea trifolia NJ-USA
22944 CBS 217.76 Fusarium proliferatum Cymbidium sp. Germany
25226 BBA 69662 Fusarium mangiferae Mangifera indica India
25331 CBS 405.97 Fusarium circinatum Pinus radiata CA-USA
25486 CBS 258.52 Fusarium xylarioides Coffea sp. Ivory Coast
28387 PD 90/1377 Fusarium commune Unknown The Netherlands
31011 BBA 69079 FIESC 12-a (Fusarium sp.) Thuja sp. Germany
31041 LI #95 Fusarium virguliforme Glycine max IL-USA
31084 PH-1 = CBS 123657 Fusarium graminearum Zea mays MI-USA
34936 CBS 123668 Fusarium oxysporum f. sp. lycopersici Solanum lycopersicum The Netherlands
36148 CBS 109638 Fusarium buxicola Buxus sp. Belgium
36575 CBS 976.97 FIESC 20-b (Fusarium sp.) Juniperus chinensis HI-USA
37021 FRC L-0110 Fusarium cf. lateritium Coffea sp. New Guinea
45880 VanEtten 77-13-4 FSSC 11-c (Fusarium solani f. sp. pisi) Pisum sativum Unknown
54149 JAS #481 (5005-08 canker) Fusarium sp. (Fsp-1) Torreya taxifolia FL-USA (Aspalaga)
54150 JAS #499 (4005-08 recently dead) Fusarium sp. (Fsp-1) Torreya taxifolia FL-USA (Gregory House)
54151 JAS #510 (5003-08 shoot dieback) Fusarium sp. (Fsp-1) Torreya taxifolia FL-USA (Aspalaga)
54152 JAS #542 (5006-09 canker) Fusarium sp. (Fsp-1) Torreya taxifolia FL-USA (Aspalaga)
54153 JAS #545 (5003-08 canker) Fusarium sp. (Fsp-1) Torreya taxifolia FL-USA (Aspalaga)
54154 JAS #587 (4008-08 canker) Fusarium sp. (Fsp-1) Torreya taxifolia FL-USA (Gregory House)
54155 JAS #596 (4025-09 canker) Fusarium sp. (Fsp-1) Torreya taxifolia FL-USA (Gregory House)
a NRRL, Agricultural Research Service Culture Collection, Peoria, IL.
b ATCC, American Type Culture Collection, Manassas, VA; BBA, Biologische Bundesanstalt fűr Land-und Forstwirtschaft, Institute fűr Mikrobiologie,
Berlin, Germany; CBS, CBS-KNAW Fungal Biodiversity Center, Utrecht, The Netherlands; FRC, Fusarium Research Center, Department of Plan
Pathology, The Pennsylvania State University, University Park, PA; JAS, Jason A. Smith, University of Florida, Gainesville, FL.
c Strains of formae speciales (f. sp.) within the Fusarium solani species complex (FSSC) represent four phylogenetically distinct species. NRRL 13622 and
37021 are listed as Fusarium cf. lateritium because it is unclear which of these two species, if any, is authentic for this species.
636 Plant Disease / Vol. 95 No. 6
quenced (85.7%). The most common species isolated was Fsp-1,
isolated from 28.5% of all samples and 51.6% of all trees. This was
followed by Pestalotiopsis sp. at 8.8% of all samples and 22.6% of
all trees sampled. The remainder of the isolates could not be identi-
fied based on rDNA-ITS sequences (23.2% of all samples and
70.9% of all trees) or were considered to be nonpathogenic fungi
(24.2% of all samples and 54.4% of all trees). A second unidenti-
fied Fusarium species (Fsp-2) based on BLASTn searches com-
prised 1.0% of all samples and 3.2% of all trees sampled.
Cankers from five trees were sampled at the TNC-Aspalaga
Tract, resulting in 14 isolates from which ITS rDNA sequence data
were obtained. The most common species isolated were Fsp-1
(21.4% of all samples and 14.3% of all trees sampled) and Pestalo-
tiopsis sp. (21.4% of all samples and 14.3% of all trees sampled).
The remainder of the isolates could not be identified using the
rDNA-ITS sequence data (42.9% of all samples and 100% of all
trees sampled) or appeared to represent saprophytic species (14.3%
of all samples and trees sampled).
Cankers from 10 trees were sampled at the TNC-Sweetwater
tract, from which ITS rDNA sequence data were obtained from the
24 isolates recovered. The most commonly isolated species was
Fsp-1, isolated from 33.4% of all samples and 40.0% of all trees
sampled. This was followed by Pestalotiopsis sp. at 20.9% of all
samples and 30.0% of all trees sampled. The remainder of the iso-
lates could not be identified to species using the ITS rDNA se-
quence data (16.6% of all samples and 40.0% of all trees sampled),
or they appeared to represent saprophytes (12.5% of all samples
and 30.0% of all trees sampled). The rest of the samples were
identified as known plant pathogens (B. obtusa, F. cf. lateritium,
members of the FSSC [12], and L. theobromae) (16.6% of all iso-
lates and 30.0% of all sampled trees), but not found at other loca-
Inoculation experiments. No cankers formed following inoc-
ulation with B. obtusa, F. cf. lateritium, Fusarium sp-2 (Fsp-2), L.
theobromae, and Pestalotiopsis sp. in IE1. The FSSC isolate and
Fusarium sp-1 (Fsp-1) caused cankers (% stem girdle; % SG =
100%) that caused mortality above the inoculation point after 6
months (Fig. 3C and D). Since Fs-Tt1 was infrequently recovered
in this study, additional experiments focused on Fsp-1. In IE2, all
three isolates of Fsp-1 induced cankers on all plants. Cankers were
not observed on the negative controls. The average % SG for iso-
late NRRL 54152 = 542 (65.0%), for NRRL 54154 = 587 (65.0%),
and for NRRL 54155 = 596 (80.0%). One of each of the two repli-
cates of isolates NRRL 54154 and 54155 caused mortality after 6
months. Sporodochia of Fsp-1 were observed on the cankered tis-
sue at approximately 4 months postinoculation. In IE3, all three
isolates of Fsp-1 tested caused cankers on all plants inoculated;
cankers were not induced on the plants included as negative con-
trols. The average % SG for all isolates inoculated onto the larger
plants was 16.9% (control = 0%) and on the smaller plants was
84.1% (control = 0.0%). Isolates NRRL 54149 = 481 and NRRL
54152 = 542 both caused sapling mortality after 6 months. After 4
months, cankers that formed from the three individual inoculation
points began to coalesce to form large single cankers (Fig. 3A and
B). For all inoculation experiments, Fsp-1was recovered from the
advancing canker margin on each plant at the end of the experi-
ment. The identity of the fungi recovered was determined to be
Fsp-1 based on analyses of their ITS rDNA sequence.
Molecular phylogenetics. DNA sequence data were obtained
from portions of three nuclear genes to identify the Florida torreya
pathogen (Fsp-1) and to characterize its genetic diversity (Table 2).
ITS rDNA sequences of the six isolates sequenced (NRRL 54149–
54151, 54153–54155) were identical and yielded an alignment of
453 nucleotide positions. A search of GenBank (http://www.ncbi., using one of the ITS rDNA sequences as the query,
identified Fusarium lateritium BBA 65675 (AY188920, 96% iden-
tity), F. buharicum (FBU34581, 97% identity), and Fusarium sp.
IP-87 (DQ780424, 97% identity) as the best matches. Partial EF-
sequences of the seven isolates (NRRL 54149-54155) yielded
an alignment of 689 bp. A branch-and-bound search of the EF-1
dataset identified four equally most parsimonious trees four steps
in length. Five unique haplotypes were identified among the seven
sequences (Fig. 4). BLAST searches of GenBank and the FUSA-
RIUM-ID database (; 5),
using a partial EF-1
sequence as the query, failed to identify any
sequences producing significant alignments. This query identified
an EF-1
sequence of F. redolens NRRL 52619 (GU250581) with
the best maximum identity, but at only 86%. Similar BLAST
searches of GenBank, using a partial RPB2 sequence as the query,
also identified sequences with a maximum identity of only 86% as
the top hits (ex., F. concolor EF470115 and F. brachygibbosum
GO505482). The results of these three BLAST searches, coupled
with comparisons of the partial EF-1
and RPB2 gene sequences
with more inclusive fusarial databases of these two genes (K.
O’Donnell, unpublished), strongly indicated that the Florida tor-
reya pathogen represented a novel species of Fusarium.
To assess phylogenetic relationships of the Florida torreya
pathogen within Fusarium, MP and ML analyses were conducted
on partial RPB2 sequences (1,772 bp alignment) of five Fsp-1 iso-
lates together with a phylogenetically diverse set of 19 Fusarium
spp.; over half of the latter have been reported to be tree pathogens
(Table 2; Fig. 5). The results of these analyses were highly concor-
dant in placing the Florida torreya pathogen as a novel phylo-
genetically distinct species representing one of the earliest diverg-
ing lineages within the Gibberella clade of Fusarium.
Field studies indicate that the existing Florida torreya population
is severely affected by canker disease. However, linking any cur-
rent threat, including CDFT, with the historical decline of this criti-
cally endangered species is difficult to accomplish. Although sev-
eral authors hypothesized the role of a pathogen in the initial
decline of Florida torreya (2,3,22), no pathogen was conclusively
demonstrated to be the cause of decline. The rapid die off and
subsequent windthrow of dead trees (21) suggests the involvement
of a root pathogen (e.g., Phytophthora cinnamomi); however, no
root pathogen has been shown to cause significant damage to
Fig. 1. Typical stem cankers (yellow arrows) observed on main stem of tree and
basal sprouts of Torreya taxifolia at natural sites.
Fig. 2. Canker incidence in eight Florida torreya sites surveyed during 2008 to
2010. Letters above bars represent homogenous subsets (Duncan’s test, P < 0.05).
Plant Disease / June 2011 637
Fig. 3. Symptoms on cultivated plants inoculated with Fusarium sp. (Fsp-1). A, Cankers coalescing; B, bark scraped away to reveal lesion; C, mortality of inoculated seedling;
D, seedling with stem mortality above inoculation point and basal sprouting. Symptoms observed at 4 weeks (A and B) and 6 weeks (C and D) postinoculation, respectively.
638 Plant Disease / Vol. 95 No. 6
Florida torreya. Moreover, the fact that basal sprouts frequently de-
velop from older root systems suggests that the roots are not cur-
rently affected by disease.
Inoculation experiments verify that CDFT is caused by an un-
described Fusarium sp. Although Fusarium spp. have been
implicated as causal agents of disease of Florida torreya in the past,
none has been demonstrated to either induce cankers and stem
mortality or cause symptoms that are similar to those observed in
the field. A pathogen reported as F. lateritium caused leaf spots on
Florida torreya, but apparently not cankers (3). The novel
Fusarium species has not been detected in previous pathology
studies of Florida torreya. However, because published studies on
CDFT did not include molecular phylogenetic data, it is possible
that Fsp-1 was misidentified as a described species using morpho-
logical species recognition.
Analyses of multilocus DNA sequence data, which included
BLAST searches of GenBank and the FUSARIUM-ID databases
(5), phylogenetic analyses of partial RPB2 gene sequences, and
DNA sequence comparisons with more inclusive EF-1
and RPB2
gene sequence databases (K. O’Donnell, unpublished data),
strongly suggest the Florida torreya pathogen represents a novel,
phylogenetically distinct species within Fusarium (25). Phyloge-
netic placement of this novel pathogen as one of the earliest diverg-
ing lineages within the Gibberella clade was greatly facilitated by
the use of partial RPB2 sequence data, which has a significant
advantage over ITS rDNA and partial EF-1
data, in that it can be
easily aligned across the phylogenetic breadth of Fusarium (11).
ITS rDNA sequence data have low utility within Fusarium because
highly divergent paralogs or xenologs have been reported within
the Gibberella fujikuroi and F. oxysporum species complexes and
because it contains relatively little phylogenetic signal (10). By
way of contrast, RPB2 sequence data are highly informative phy-
logenetically, as evidenced by the large number of parsimony
informative characters in the dataset analyzed in the present study
(Fig. 1) and in previously published studies of other fusaria
Florida torreya faces numerous challenges to its future survival
in its natural habitat. In addition to the canker disease, deer rou-
tinely cause damage to stems from antler rubbing. It is unclear
whether they are attracted by the tree’s aroma or seek out Florida
torreya for some other unknown reason. Whether the wounds
caused by deer serve as infection courts for pathogens, including
the new Fusarium sp. (Fsp-1), is unclear and warrants further
study. Additionally, since lesions on the larger plants in IE3 re-
sulted in less stem girdling and no mortality, the host response to
infection, particularly under different stress conditions, needs to be
investigated. In addition to more research on the biology and man-
agement of CDFT, more work is needed to assess the various fac-
tors involved in decline of Florida torreya and how the species can
be protected from extinction.
We thank the Florida Parks Department staff and numerous volunteers who
were instrumental in carrying out the surveys for Florida torreya. We also thank
Stacy Sink for generating the DNA sequence data reported in this study and
Nathane Orwig for collecting the DNA sequence data in the NCAUR DNA core
facility. Mention of firm names or trade products does not imply that they are
endorsed or recommended by the U.S. Department of Agriculture over other
firms or similar products not mentioned.
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... Florida torreya (Torreya taxifolia Arn.) is a critically endangered conifer listed by the U.S. Fish and Wildlife Service. The species has a limited native range consisting of ravines along the Apalachicola River, distributed in Liberty and Gadsden counties in Florida, USA, and in Decatur county Georgia (Smith et al., 2011). Historically, Florida torreya grew to 15-20 m and was relatively abundant, comprising about 14% of dominant (canopy) ravine trees, but experienced a dramatic decline from the late 1950s to early 1960s, suffering a ~99% loss in population size that was thought to be caused by a disease (Schwartz, Hermann, & van Mantgem, 2000). ...
... Historically, Florida torreya grew to 15-20 m and was relatively abundant, comprising about 14% of dominant (canopy) ravine trees, but experienced a dramatic decline from the late 1950s to early 1960s, suffering a ~99% loss in population size that was thought to be caused by a disease (Schwartz, Hermann, & van Mantgem, 2000). A survey by Smith et al. (2011) found 225 individuals with average heights from ground level of 78-263 cm and canker incidence of 71%-100% for the populations sampled. The cause of the canker disease was identified as F. torreyae, a previously undescribed species, and is currently a limiting factor in tree development (Aoki, Smith, Mount, Geiser, & O'Donnell, 2013;Smith et al., 2011). ...
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... Exotic fungal pathogens present a particular risk due to their rapid emergence, low resistance in host populations, and limited surveillance infrastructure for detection. Invasive fungal pathogens are linked to major ecological disasters, including near extinctions of forest tree species, such as the American chestnut due to Cryphonectria parasitica (24) and Florida torreya due to Fusarium torreyae (25). They have also caused a global decline of amphibian populations due to chytridiomycosis caused by Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans (2,26). ...
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As one of the world's most threatened coniferous trees, Torreya taxifolia has attracted the attention of a variety of conservation organizations and researchers across its native country of the USA and beyond. The current status of the species is one of very poor individual health with trees being very short and failing to produce seeds, while being confined to a restricted range in the south-eastern US. The status reflects a precipitous decline that is widely reported to be influenced by a number of threats including over-exploitation primarily for construction materials, modifications to natural systems and most notably, a pathogenic fungus which results in the poor growth and lack of reproduction and has been named Fusarium torreyae. However, the species is a glacial relict at the southernmost extent of its post-glacial range and as such, is also likely to suffer from the impacts of climate change. Current conservation actions include attempts to carefully monitor individual trees and document the impacts of catastrophes such as Hurricane Michael in 2018 and ‘cage’ them to prevent damage from deer; comprehensive collections of cuttings and seed to be grown in botanic gardens, and a range of translocation attempts to either bolster the species in its native range, or identify new sites in which it might thrive.
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Fusarium zanthoxyli and F. continuum are sister taxa that are the etiological agents of canker disease of prickly ash (Zanthoxylum bungeanum) in northern China. These two pathogens, together with F. torreyae, the causal agent of canker disease of the critically endangered conifer Florida torreya (Torreya taxifolia) from northern Florida and southwestern Georgia, constitute a novel clade, the F. torreyae species complex. To assess their reproductive mode, a polymerase chain reaction (PCR) assay targeting the MAT1-1 and MAT1-2 idiomorphs was designed and validated, using MAT sequences mined from the whole-genome sequence of the three F. torreyae clade pathogens and several closely related fusaria. Results of the MAT idiomorph PCR assay indicated that isolates of the three pathogens were MAT1-1 or MAT1-2. When MAT1-1 and MAT1-2 isolates of each species were crossed on carrot agar, all of the F. zanthoxyli (N = 30) and F. continuum (N = 3) isolates tested were female fertile, yielding mature perithecia with viable ascospores. By comparison, only one pairing of the five different isolates of F. torreyae produced perithecia; however, the majority of the asci in this cross aborted or produced fewer than eight ascospores. Of the three temperatures tested (i.e., 22, 25, and 27 C), the optimal temperature for perithecium production was 22–25 C in F. zanthoxyli and 25 C in F. continuum and F. torreyae. Ascospore progeny from three separate crosses of F. zanthoxyli and F. continuum and one cross of F. torreyae were genotyped to assess whether they were the products of genetic recombination and sexual reproduction. Genotyping of 34–40 progeny from the F. zanthoxyli and F. continuum crosses confirmed that they were the products of sexual reproduction. However, only 36% of the progeny in the F. torreyae cross were recombinant, which was roughly half of the nonparental progeny expected with three markers segregating.
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The Florida torreya (Torreya taxifolia Arn.) has decreased to near extinction during the past 30 yr, allegedly as the result of a fungal disease induced by environmental stress. Increasing regional temperatures, micro-climatic warming, drought, and soil nutrient stress are among the environmental changes proposed as triggers for the decline of T. taxifolia. These putative disease inciting mechanisms were evaluated through historical observations of the magnitude of environmental change, as well as physiological and growth experiments used to assess the relative sensitivity of T. taxifolia to environmental change. Historical evidence of climatic warming, regionally or locally, is lacking. Likewise, physiological experiments failed to support the temperature change hypothesis. A drought, concurrent with the timing of the decline, was not notably severe and T. taxifolia was observed to be relatively drought tolerant. Greenhouse growth experiments demonstrated that low soil nutrition is not likely to limit growth in T. taxifolia. Another hypothesis states that fire suppression in the surrounding uplands triggered a disease epidemic. This hypothesis states that smoke deposition in ravines may limit foliar pathogen populations and that a chronic increase of foliar pathogens, associated with fire suppression, triggered the decline of T. taxifolia. Our experiments suggest that foliar pathogens may impose health risks to T. taxifolia and that one very common foliar fungal associate is susceptible to smoke. Decreased light levels, as a result of increased woody biomass in the absence of fire, may also increase plant stress, leading to increased disease incidence. While the low light levels characteristic of field situations limited T. taxifolia growth in the greenhouse, light level was not correlated with growth, or vigor, in the field. These results suggest that the fire suppression hypothesis is, at least, plausible and requires further examination.
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Torreya taxifolia (Arn.), an endangered conifer, is believed to be failing in its environment because of poor growth. Poor growth has been hypothesized to be a result of disease infection, yet a primary disease agent has remained elusive. We compared the growth of T. taxifolia to a close congener, T. californica (Torrey), to determine if T. taxifolia is growing less vigorously in its environment than would be expected under the assumption that the two species should exhibit similar growth patterns. We found that growth patterns do not statistically differ between the two species although T. taxifolia shows a slightly higher growth than T. californica by most measures. With no reason to believe that T. californica is failing in its environment, we cannot reject the hypothesis that the currently observed patterns of growth in T. taxifolia are normal for this species. Tree rings were sampled from dead and downed logs that date from the time of the decline. Tree rings show frequent periods of suppression and release consistent with a tree responding to variation in light. In addition, trees planted in high light treatment expanded a terminal bud and grew in height more frequently than those in low light treatments. Our results are consistent with the hypothesis that low light is a primary environmental feature limiting growth in T. taxifolia. These observations argue against, but do not reject, the hypothesis that low growth rate is a result of disease stress.
Phylogenetic relationships of the phyto-pathogenic Gibberella fujikuroi species complex were investigated by maximum parsimony analysis of DNA sequences from multiple loci. Gene trees inferred from the β-tubulin gene exons and introns, mitochondrial small subunit (mtSSU) rDNA, and 5′ portion of the nuclear 28S rDNA were largely concordant, and in a combined analysis, provide strong statistical support for a phylogeny consistent with species radiations in South America, Africa, and Asia. These analyses place the American clade as a mono-phyletic sister-group of an African-Asian clade. Africa is the most phylogenetically diverse area examined with 16 species, followed by America (12 species) and Asia (8 species). The biogeographic hypothesis proposed from the phylogenetic evidence is based primarily on the formation of natural barriers associated with the fragmentation of the ancient super-continent Gondwana. Discordance of the nuclear ribosomal internal transcribed spacer (ITS) based tree with gene trees from the other loci sequenced is due to nonorthologous ITS2 sequences. The molecular evidence suggests the divergent ITS2 types were combined by an ancient interspecific hybridization (xenologous origin) or gene duplication (paralogous origin) that predates the evolutionary radiation of the G. fujikuroi complex. Two highly divergent nonorthologous ITS2 types designated type I and type II were identified and characterized with conserved ITS and ITS2 type-specific polymerase chain reaction (PCR) primers and DNA sequence analysis. Only the major ITS2 type is discernible when conserved ITS primers are used; however, a minor ITS2 type was amplified from every strain tested with type-specific PCR primers. The evolutionary pattern exhibited by the major ITS2 type is homoplastic when mapped onto the species lineages inferred from the combined nuclear 28S rDNA, mtSSU rDNA, and β-tubulin gene sequences. Remarkably, the data indicate the major ITS2 type has switched between a type I and type II sequence at least three times during the evolution of the G. fujikuroi complex, but neither type has been fixed in any of the 45 species examined. Twenty-six of the 45 species included in this study represent either new species (23 species), new combinations (F. bulbicola and F. phyllophilum), or a rediscovered species (F. lactis). The results further indicate that traditional sectional and species-level taxonomic schemes for this lineage are artificial and a more natural classification is proposed.
One of the greatest impediments to the study of Fusarium has been the incorrect and confused application of species names to toxigenic and pathogenic isolates, owing in large part to intrinsic limitations of morphological species recognition and its application. To address this problem, we have created FUSARIUM-ID v. 1.0, a publicly available database of partial translation elongation factor 1-alpha (TEF) DNA sequences, presently representing a selected sample of the diversity of the genus diversity, with excellent representation of Type-B trichothecene toxin producers, and the Gibberella fujikuroi, Fusarium oxysporum and F. solani species complexes. Users can generate sequences using primers that are conserved across the genus, and use the sequence as a query to BLAST the database, which can be accessed at http:// fusarium. cbio. psu. edu, or in a phylogenetic analysis. Correct identification of a known species in these groups often can be performed using this gene region alone. This growing database will contain only vouchered sequences attached to publicly available cultures. In the future, FUSARIUM-ID will be expanded to include additional sequences, including multiple sequences from the same species, sequences from new and revised species, and information from additional genes.
The first handbook to include detailed information on all 615 conifers, temperate as well as tropical, this encyclopedic work offers users as diverse as ecologists, gardeners, foresters and conservationists the accumulated knowledge of these trees obtained in 30 years of academic research, presented in an easily searchable format. © 2010 by Koninklijke Brill NV, Leiden, The Netherlands. All rights reserved.
For the first time in over 20 years, a comprehensive collection of photographs and descriptions of species in the fungal genus Fusarium is available. This laboratory manual provides an overview of the biology of Fusarium and the techniques involved in the isolation, identification and characterization of individual species and the populations in which they occur. It is the first time that genetic, morphological and molecular approaches have been incorporated into a volume devoted to Fusarium identification. The authors include descriptions of species, both new and old, and provide protocols for genetic, morphological and molecular identification techniques. The Fusarium Laboratory Manual also includes some of the evolutionary biology and population genetics thinking that has begun to inform the understanding of agriculturally important fungal pathogens. In addition to practical how-to protocols it also provides guidance in formulating questions and obtaining answers about this very important group of fungi. The need for as many different techniques as possible to be used in the identification and characterization process has never been greater. These approaches have applications to fungi other than those in the genus Fusarium. This volume presents an introduction to the genus Fusarium, the toxins these fungi produce and the diseases they can cause. The Fusarium Laboratory Manual is a milestone in the study of the genus Fusarium and will help bridge the gap between morphological and phylogenetic taxonomy. It will be used by everybody dealing with Fusarium in the Third Millenium. -W.F.O. Marasas, Medical Research Council, South Africa.