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Uptake and translocation of metals and nutrients in tomato grown in soil polluted with metal oxide (CeO2, Fe3O4, SnO2, TiO2) or metallic (Ag, Co, Ni) engineered nanoparticles


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The influence of exposure to engineered nanoparticles (NPs) was studied in tomato plants, grown in a soil and peat mixture and irrigated with metal oxides (CeO2, Fe3O4, SnO2, TiO2) and metallic (Ag, Co, Ni) NPs. The morphological parameters of the tomato organs, the amount of component metals taken up by the tomato plants from NPs added to the soil and the nutrient content in different tomato organs were also investigated. The fate, transport and possible toxicity of different NPs and nutrients in tomato tissues from soils were determined by inductively coupled plasma-optical emission spectrometry (ICP-OES). The tomato yield depended on the NPs: Fe3O4-NPs promoted the root growth, while SnO2-NP exposure reduced it (i.e. +152.6 and -63.1 % of dry matter, respectively). The NP component metal mainly accumulated in the tomato roots; however, plants treated with Ag-, Co- and Ni-NPs showed higher concentration of these elements in both above-ground and below-ground organs with respect to the untreated plants, in addition Ag-NPs also contaminated the fruits. Moreover, an imbalance of K translocation was detected in some plants exposed to Ag-, Co- and Fe3O4-NPs. The component metal concentration of soil rhizosphere polluted with NPs significantly increased compared to controls, and NPs were detected in the tissues of the tomato roots using electron microscopy (ESEM-EDS).
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Uptake and translocation of metals and nutrients in tomato grown
in soil polluted with metal oxide (CeO
or metallic (Ag, Co, Ni) engineered nanoparticles
Livia Vittori Antisari &Serena Carbone &
Antonietta Gatti &Gilmo Vianello &Paolo Nannipieri
Received: 8 October 2013 /Accepted: 24 August 2014
#Springer-Verlag Berlin Heidelberg 2014
Abstract The influence of exposure to engineered nanopar-
ticles (NPs) was studied in tomato plants, grown in a soil and
peat mixture and irrigated with metal oxides (CeO
) and metallic (Ag, Co, Ni) NPs. The morpholog-
ical parameters of the tomato organs, the amount of compo-
nent metals taken up by the tomato plants from NPs added to
the soil and the nutrient content in different tomato organs
were also investigated. The fate, transport and possible toxic-
ity of different NPs and nutrients in tomato tissues from soils
were determined by inductively coupled plasma-optical emis-
sion spectrometry (ICP-OES). The tomato yield depended on
the NPs: Fe
-NPs promoted the root growth, while SnO
NP exposure reduced it (i.e. +152.6 and 63.1 % of dry
matter, respectively). The NP component metal mainly accu-
mulated in the tomato roots; however, plants treated with Ag-,
Co- and Ni-NPs showed higher concentration of these ele-
ments in both above-ground and below-ground organs with
respect to the untreated plants, in addition Ag-NPs also con-
taminated the fruits. Moreover, an imbalance of K transloca-
tion was detected in some plants exposed to Ag-, Co- and
-NPs. The component metal concentration of soil rhi-
zosphere polluted with NPs significantly increased compared
to controls,and NPs were detected in the tissues of the tomato
roots using electron microscopy (ESEM-EDS).
Keywords Nanoparticles .Tomato (Lycopersicon esculentum
Mill.).Pollution .Translocation .Electron microscopy .
Inductively coupled plasma-optical emission spectrometry
Nanotechnology is an emerging field with multiple potential-
ities and applications (Remédios et al. 2012). Engineered
nanomaterials (ENMs) are defined as materials with at least
one dimension under 100 nm (American Society for Testing
and Materials 2006; British Standards Institution 2007;Sci-
entific Committee on Emerging and Newly Identified Health
Risks 2007). Their small size provides a high surface/volume
ratio, leading to high reactivity, and both of these factors can
affect the strength and the electrical and optical properties of
the systems in which they are used (Farré et al. 2011). This
characteristic makes ENMs particularly suitable for use in the
cosmetics, medicine, pharmacy, food agriculture, high-tech,
aerospace, electronics and defence industries. ENMs can be
classified according to their size (e.g. with three dimensions
below 100 nm, they are named nanoparticles, NPs), morphol-
ogy (flatness, sphericity and aspect ratio) and composition
(made up of single material or several materials) (Remédios
et al. 2012). The production, use and disposal of NPs will
inevitably lead to their release into the air, water and soil (Lin
and Xing 2007; Vittori Antisari et al. 2011a) with a certain
amount of exposure to humans, and the risk posed by
NPs is currently subject to a robust debate (Ruffini
Castiglione et al. 2011).
Soil is rich in natural NPs, such as clay, iron oxides and
organic matter, although it could also be a preferential sink of
engineered NPs due to direct (e.g. agricultural practices) or
Responsible editor: Elena Maestri
L. Vittori Antisari :S. Carbone (*):G. Vianello
Dipartimento di Scienze Agrarie, Alma Mater Studiorum,
Università di Bologna, Viale Fanin 40 40127, Bologna, Italy
A. Gatti
Nanodiagnostics, Srl Via Enrico Fermi 1/L, 41057 San Vito di
Spilamberto, Modena, Italy
P. Nannipieri
Dipartimento di Scienze Delle Produzioni Agroalimentari e
DellAmbiente, Università di Firenze, Piazzale delle Cascine,
28-50144 Florence, Italy
Environ Sci Pollut Res
DOI 10.1007/s11356-014-3509-0
indirect uses (e.g. via sewage treatments plants, aerial deposi-
tion or waste handling, Gottschalk et al. 2009). NPs can enter
the food chain through the soil in which crops are grown
(Pidgeon et al. 2009) and increase the risk of NP bioaccumu-
lation in the animal and human food chain (Zhu et al. 2008).
Rico et al. (2011) discussed the effects of the adsorption,
translocation and accumulation of NPs in food crops,
highlighting that these processes depend on the plant species
and the size, type, chemical composition and stability of NPs.
The accumulation of NPs and/or of component metal in plant
tissues is poorly known, and their effects on different crops
have been mainly studied in vitro: with aqueous suspensions
(Lin and Xing 2007;KumariandMukherjee2009;
Stampoulis et al. 2009; Yang and Watts 2009; El-Temsah
and Joner 2010; Lopez-Moreno et al. 2010), Hoagland solu-
tion (Schwabe et al. 2013) and agar culture media (Lee et al.
2008). Few experiments have been performed with soil (El-
Temsah and Joner 2010; Sheykhbaglou et al. 2010;Priester
et al. 2012;Wangetal.2012; Hernandez-Viezcas et al. 2013;
Servin et al. 2013). Toxicological experiments carried out in
hydroponic systems are very useful for understanding NP
behaviour in a standardized media; however, it could be
unrealistic since the physico-chemical and biological charac-
teristics of the soil can affect the amount of NPs available to
soil biota and crops (Vittori Antisari et al. 2011b,2012,2013;
Rico et al. 2011). For an excellent review on nanomaterials
and full life cycle of plant, see Gardea-Torresdey et al. (2014).
Tomato (L. esculentum Mill.) is one of the most important
vegetable crops worldwide because of its high consumption
(Ouzounidou et al. 2008). We therefore chose it as a model
plant to monitor the effects of different NPs added to a model
soil through the irrigation water.
The aim of this work was to evaluate the influence of
different metal oxide (CeO
) and metallic
(Ag, Co, Ni) NPs on the following: (i) the morphological
parameters of tomato organs (e.g. dry weight and plant
height), (ii)NP component metal taken upby the tomato plant
(namely, Ag, Ce, Co, Fe, Ni, Sn and Ti) and (iii) the content of
nutrients (Ca, Mg, K, Na, P and S) in different tomato organs.
The fate, transport and possible accumulation in tomato or-
gans of different NPs as well as the nutrients were determined
by inductively coupled plasma-optical emission spectrometry
Material and methods
NP characterization The metal oxide NPs (CeO
) were provided by Nanostructured & Amorphous Ma-
terials, Inc (Houston, USA) and have a purity of at least 98 %.
According to the manufacturer, their specific surface area
(SSA) was 815, >40 and 14 m
/g for CeO
-, Fe
-NPs, respectively; the particle sizes, calculated by the
SSA and transmission electron microscopy, were 50105, 20
30 and 61 nm, respectively.
The Ag-NPs were obtained from Polytech (Germany, type
WM 1000-c), supplied as a 1,000 mg L
in a deionized water
suspension of metallic silver (Ag) with a NP size between 1
and 10 nm. Both Co- and Ni-NPs were obtained from Nano-
structured & Amorphous Materials, Inc. (USA) as a powder,
with a NP size of 28 and 62 nm, respectively. The TiO
were supplied from Tal Materials, Inc. (USA), as a powder
with an average size between 20 and 160 nm.
The NPs were suspended in deionized water (solution of
for each metal element of NPs) and dispersed by
ultrasonic vibration (100 W, 40 kHz) for 30 min, except for
the silver, which was used as is. Then, 20 μgmL
of NP
elements was added to the soil near the plant root before
adding the irrigation water. Fertilizer was not used during
the experiment.
Experimental design The growth experiment was carried out
at the Agricultural Science Department of Bologna University
(Italy) in a climate-controlled greenhouse under full sunlight
from March 26 to August 4, 2012 (photoperiod 11.5/13 h
winter/summer); this period corresponds to the vegetative
cycle of tomato (L. esculentum cv. Cilao F1). The nominal
maximum temperature in the greenhouse was set at 28 °C. The
seedlings (about 10 cm high) were placed in pots (5 L each
with 5 kg of soil) containing a model soil made of natural soil
and peat (1:4 v/v) which represents an excellent growth me-
dium due to the high moisture and nutrient-holding capacity
with a uniform and slow breakdown rate of physical structure
(Ball et al. 2000).A quartz and feldspar sand layer (4 cm) was
placed at the bottom of the pot to maintain the drainage.
A total of 48 pots (6 pots for each NPs) were placed in a
randomized block; after 2 weeks of adaptation, the seedlings
were spiked with Ag-, CeO
-, Co-, Fe
-, Ni-, SnO
-NP solutions at 20 μgmL
of NP component metal
once per week, twice from the 13th week, to simulate a
chronic exposure to NPs supplied with irrigation. For the
control test, only water was supplied.
Plant sampling and vegetal tissues analysis At the end of the
growing cycle (130 days), each tomato plant was separated
into the aerial part (i.e. stem and leaves) and the below-ground
part. The above-ground plant was washed with deionized
water and oven-dried at 60 °C until constant weight to deter-
mine dry mass and water content. The fruits were also collect-
ed, washed, frozen at 80 °C and thenlyophilized. Dry tissues
of different organs of each tomato were ground and digested
using a nitric acid and oxygen peroxide solution in a micro-
wave oven (Start D 1200, Milestone) according to the US
Environmental Protection Agency (USEPA 2009) method,
modified by Vittori Antisari et al. (2011a). An approximately
0.4 g subsample of plant tissue was treated with 8 mL of
Environ Sci Pollut Res
concentrated plasma-pure nitric acid (65 % Suprapur grade,
Merck) plus 2 mL of hydrogen peroxide (30 % for electronic
use, Carlo Erba,). The mineralization was carried out in PTFE
bombs in the microwave oven, and the content of nutrients
(Ca, Mg, K, Na, P and S) and the component metal (Ag, Co,
Ni, Ce, Sn, Ti and Fe) in the leaves, stems, fruits and roots was
quantified by inductively coupled plasma spectrometry (ICP-
OES, Spectro Arcos, Ametek). Reagent blanks and interna-
tional reference materials (olive leaves BCR-CRM 062) were
analyzed to validate the method. In addition, standard solu-
tions (0.5 mg L
Ag, Ce, Co, Ni and Sn) were analyzed every
ten samples for quality control/quality assurance purposes.
Soil sampling and analysis The soil of three tomato plants
was sampled as follows: A Plexiglas® cylinder was inserted in
the soil, and a column of 12 cm was sampled. The soilcolumn
was divided into four layers, each one 3 cm deep, and the
deepest sample was sand.
The root system of each plant was removed from the bulk
soil, and the rhizosphere soil samples were then obtained by
shaking the roots after soil drying (Turpault 2006) and care-
fully collecting the aggregate still attached to the roots. After-
wards, the roots were washed with distilled water and oven-
dried to perform dry mass and ICP-OES analysis (see Plant
sampling and vegetal tissues analysissection). No further
washing procedure (e.g. acidulated water) was carried out;
therefore, in ICP-OES analysis, both NP surface adsorbed
and taken up by the roots were determined.
The rhizosphere soil samples were air dried and finely
ground with an agate mill. The metal content was determined
according to Vittori Antisari et al. (2013). Briefly, the soil
(0.25 g) was treated with aqua regia (2 mL 65 % HNO
6 mL 37 % HCl, Suprapur grade, Carlo Erba) in the micro-
wave oven, and the metal concentrations were deter-
mined by ICP-OES. The analysis of each sample was
replicated three times and compared with analyses of
the international reference materials (BCR 141) and
laboratory internal standards (MO and ML), which was
run after every ten samples to check changes in sensi-
tivity. Reagents blanks were also determined.
Translocation index The translocation index (TI) was calcu-
lated to synthesize the capability of the species to translocate
nutrients and pollutants from roots to shoots (Paiva et al.
2002), according to the following equations:
TI ¼DMLðÞ=DMR þDMS þDMLðÞ100 and
where DMR, DML and DMS are the element concentrations
as a function of dry matters of roots, leaves and stem,
ESEM analysis Detection of NPs inside the tissues of tomato
organs was carried out by field emission gun-environmental
scanning electron microscope (FEG-ESEM) investigations
and energy-dispersive X-ray spectroscopy (EDS) analyses to
classify univocally the engineered NPs and differentiate them
from the natural particles. The electron microscope analyses
were performed in the secondary electron and backscattered
electron diffraction mode, in order to obtain information on
the morphology of the samples and on the chemical nature of
the NPs.
Statistical analysis Treatments were allocated in a completely
random design, and the data were reported as the mean of
three replicates (±standard deviations (SD)). Data were ana-
lyzed by variance and Tukeys test with the statistical package
SPSS 15.0, unless otherwise stated.
Concentration of NP component metal in rhizosphere and soil
The concentrations of NP component metals in treated soil
after dissolution in aqua regia are shown in Table 1.Generally,
the component metal concentration of soil rhizosphere pollut-
ed with NPs significantly increased (P<0.05) compared to
controls. With regard to the soil column, the concentration of
the surface layer (03 cm) increased significantly (P<0.05)
compared to the control, except for soil polluted with Fe
and TiO
-NPs. In the case of magnetite, the Fe concentration
increased by 75 % compared to the control in the 36-cm soil
layer, whereas in the case of the TiO
-NP treatment, the
deepest soil layers were more enriched (ca. 47 %) compared
to the control. In general, the amount of NP component metal
decreased with the depth, even if the lowest values were
generally determined in the 3 to 6-cm layer of the soil column.
The plants exposed to NPs showed different vegetative
growth (Table 2). Magnetite promoted the root growth (4.8 g
dry weight (d.w.)) while SnO
-NP exposure (P<0.05) reduced
it (0.7 g d.w.) with respect to the control (1.9 g d.w.); the other
NP treatments showed no significant difference compared to
the control roots. Phenotype and dry matter of the above-
ground biomass varied among treatments: Ag-NP exposure
decreased the elongation of the stem (ca. 16 %) with respect
to the control but, at the same time, increased the dry matter of
thestem(ca.28%)(P<0.05). Conversely, in plants exposed
to Co-NPs, the reduction of the stem elongation (ca. 14 %)
was associated with a decrease of the above-ground dry
Environ Sci Pollut Res
biomass compared to the control (ca. 50 and 27 % for the
stem and the leaves, respectively).
treatments determined a slight increase of the stem
elongation, not statistically significant, associated to a de-
crease of the stem and leaves dry matter (ca. 36 and
37 %, respectively).
NPs, although promoting the root growth for both
elongation and dry matter parameters, reduced significantly
the above-ground dry biomass (ca. 12 and 25 % for the
stem and the leaves, respectively).
The exposure to Ni-NPs determined a slight decrease of the
stem elongation, not statistically significant, associated to an
increase of the stem dry matter (5.6 %) as Ag-NP exposure
did, but in addition, it decreased significantly with respect to
the untreated plants the dry matter of the leaves (13.1 %).
-NP exposure reduced significantly also the above-
ground dry matter (ca. 74 and 33 % for the stem and the
leaf, respectively).
Tomato plants exposed to TiO
-NPs did not show
significant changes in root and stem elongation but
Tabl e 1 Concentration of NP component metal in the soil rhizosphere and in the different layers of the soil column
Rhizosphere Soil column (cm)
Treatment SD SD SDSDSD
Ag-NPs 26.1*** 3.2 36.9*** 0.2 9.9*** 0.09 0.9* 0.04 0.03 ns
Ag control 0.03 0.03 0.03 0.03 0.03
Co-NPs 45.6** 2.9 64.6** 0.1 8.8** 0.3 6.8 ns 0.1 7.6 ns 0.2
Co control 5.2 0.1 5.3 0.2 2.9 0.2 5.5 0.1 7.4 0.2
Ni-NPs 71.1** 5.0 32.6** 0.3 8.1 ns 0.2 23.4 ns 0.3 24.3 ns 0.9
Ni control 12.2 1.6 24.1 0.4 11.5 0.2 21.3 0.2 26.8 0.8
-NPs 37.7** 2.7 100.7** 4.1 9.2 0.7 14.6 ns 1.6 9.8 ns 5.8
Ce control 5.2 0.7 11.0 2.5 6.8 1.5 9.8 0.5 12.5 0.4
-NPs 5.6 ns 3.3 11.5 ns 0.4 10.4** 0.2 17.1 ns 0.3 16.5 ns 0.2
Fe control 5.6 0.8 12.1 0.3 5.9 0.2 12.2 0.2 16.1 0.4
-NPs 6.9** 8.3 3.0* 0.4 2.1* 0.3 1.1 ns 0.1 1.0 ns 0.2
Sn control 0.5 0.2 0.8 0.2 0.8 0.2 1.0 0.2 0.8 0.0
-NPs 95.2 ns 25.0 166.5 ns 18.8 104.6 ns 7.3 207.6* 7.3 206.5* 7.5
Ti control 138.6 8.9 198.2 112.7 138.6 6.9 142.2 0.0 140.2 6.8
One-way ANOVA and Tukeystest(P< 0.05) were used to determine statistical significance of differences between the treatment and the control means.
The Ag value of the control and the 912-cm soil layer column was equal to the detection limit (DL). The data were expressed as microgram per gram
) d.w., except for Fe (mg g
ns is not significant; SD is the standard deviation
***P<0.001; **P<0.01; *P<0.05
Tabl e 2 Effect of NPs on dry matter of roots, stems and leaves of Lycopersicon esculentum plants grown in pots
Root Stem Leaves Root elongation Plant height
Treatment g SD g SD g SD cm SD cm SD
Control 1.9 b 0.1 20.5 b 0.9 25.2 a 1.1 22 ab 1.3 98 a 3.8
Ag-NPs 1.6 b 3.3 26.2 a 1.2 24.2 a 0.9 19 b 1.5 82 b 5.3
Co-NPs 1.5 b 0.3 10.3 d 1.1 18.3 b 1.5 15 b 2.8 84 b 4.2
Ni-NPs 1.0 bc 0.3 26.1 a 1.2 12.1 d 0.9 15 b 3.2 93 ab 5.1
-NPs 2.2 ab 0.2 13.1 cd 1.4 15.7 c 0.7 23 ab 2.1 109 a 3.1
-NPs 4.8 a 0.2 18.1 c 0.8 18.9 b 1.3 25 a 2.3 106 a 3.5
-NPs 0.7 c 0.2 5.4 e 0.7 16.8 c 1.5 11 b 3.7 104 a 3.4
-NPs 1.4 b 0.1 19.2 b 1.1 18.8 b 0.8 17 b 2.1 110 a 4.1
Means followed by a different letter within a row are significantly different at P<0.05 according to the Duncans multiple range test
Environ Sci Pollut Res
showed a decrease of the leaf dry matter (25.3 %)
with respect to the control.
Metal content in tomato organs
The NP component metals were accumulated in the tomato
roots (Table 3). Generally, the concentrations of these metals
in the control tissues were lower than the instrumental detec-
tion limit (DL, see values in Table 3). Organs of tomato grown
in soil irrigated with TiO
-NPs showed no difference com-
pared to the control, and this is also true for organs of tomato
exposed to Fe
-NPs, except for fruits in which the Fe
concentration was higher than the control (116.6 and
45.5 μgg
, respectively). Generally, Ag, Co and Ni concen-
trations were higher than those of the control tomato organs,
except for fruits of plants subjected to Co-NPs and leaves and
roots of plants treated with Ni-NPs.
The amount of metals arising from NPs for each pot (μg
per pot) is shown in Table 4. The distribution of metals in the
above-ground organs of tomato changed as a function of the
NP exposure. Ag, Co and Ni accumulated in leaves with an
amount of 26.7, 22.0 and 24.6 μg per pot, respectively; Fe, Ce
and Sn accumulated in roots (2,087.0; 3.7 and 0.4 μg per pot,
respectively) and Ni in stems (16.3 μg per pot). The Ti did not
accumulate in the crop irrigated with TiO
-NPs compared to
the control. The translocation index (TI) showed that tomato
was able to translocate Ag and Co from root to above-ground
organs (Table 4), whereas the respectivetranslocation of Fe to
leaves was lower compared to the control (30 and 69.5 % in
stem and leaves, respectively); Ni accumulated in stems. No
translocation of Sn, Ti and Ce was found either in the control
or in the treated tomatoes.
ESEM analysis of tomato roots
The examination of the organ tissues of the tomato
plants to find NPs was performed by ESEM-EDS. Ex-
amples of tomato roots exposed to NPs are shown in
Figs. 1,2and 3. Ag-NPs were detected within root
cells (Fig. 1) grouped as a large cluster (from 100 to
200 nm) but also individually dispersed. Cluster
Tabl e 3 Comparison between the concentration of NP component metal of stem, leaves, root and fruit of tomato grown with or without (control) NPs
Ag Co Ni Ce Fe Sn Ti
Stem Treatment 0.2 0.01 0.4 0.001 0.9 0.1 DL 38.4 0.5 DL 1.4 0.1
Control DL DL DL DL 49.8 4.2 DL 1.7 0.1
** * ns ns
Leaves Treatment 1.1 0.05 1.2 0.05 1.3 0.3 DL 19.9 7.8 DL 3.3 0.8
Control DL DL 1.1 0.5 DL 20.5 8.6 DL 2.8 0.03
* * ns ns ns
Root Treatment 2.6 0.06 3.7 2.3 0.1 1.7 0.01 534.8 29.1 0.6 0.01 5.5 0.01
Control DL 0.2 3.2 0.3 DL 383.5 12.3 DL 7.9 0.2
** ns** *ns
Fruit Treatment 0.3 0.01 DL 0.8 0.1 DL 116.8 7.9 DL 2.9 0.4
Control DL DL DL DL 42.5 8.1 DL 5.5 0.1
One-way ANOVA and Tukeystest(P< 0.05) were used to determine statistical significance of the differences between treatment and the control means.
The data are expressed as μgg
***P<0.001;**P<0.01; *P<0.05
ns is not significant. DL was the instrumental detection limit of Ag (0.006 μgg
), for Ce (0.01 μgg
), for Co (0.0002 μgg
), for Ni (0.01 μgg
and for Sn (0.01 μgg
). SD is the standard deviation
Tabl e 4 (A) Amount of NP component metal accumulated in stem,
leaves and root expressed as microgram per pot (referred to grams of
dry substance). (B) Translocation index (TI): calculated values expressed
as the percentage of NP component metal from above-ground to below-
ground organs
Stem Treatment 5.2 4.1 16.3 998.4 26.9
Control 1.2 1.8 944.2 34.9
Leaves Treatment 26.7 22.0 24.6 240.6 62.0
Control 1.5 27.8 517.8 70.7
Root Treatment 4.2 5.6 2.5 3.7 2,087.0 0.4 7.7
Control 0.1 0.4 6.1 728.7 0.02 15.0
B Translocation index (TI; %)
TI NP Stem 14.4 12.9 37.6 30.0 27.8
Leaves 74.0 69.4 56.7 7.2 64.2
TI Ctr Stem ––5.0 43.1 28.9
Leaves ––55.4 23.6 58.6
Environ Sci Pollut Res
exposed to TiO
-NPs; TiO
-NPs were distributed
parallel to the longitudinal section of roots (Fig. 2), whereas
-NPs showed spherical clusters of different sizes (Fig. 3).
In all the spectra by EDS, the NPs were associated with soil
compounds (e.g. Al and Si).
Fig. 1 adESEM images of Ag nanoparticles in tomato roots of plants grown in soil amended with Ag-NPs. e,fEDS spectra of Ag-NPs and natural
Fig. 2 acESEM images of TiO
nanoparticles detected in root tissues of tomato ofplants grown in soil amended with TiO
-NPs. dEDSspectrumof
-NPs and natural nanoparticles
Environ Sci Pollut Res
Nutrient content in tomato organs
The average concentrations of nutrients in tomato organs
grown in soil polluted with NPs are reported in Table 5.As
seen in the table, the nutrient content is influenced by the NP
treatments in different ways with respect to the control.
Ca content in leaves changed with the treatments and can
be ranked as follows: Ag> Ni > CeO
=Co=control (Ctr) =
NPs. The highest Ca concentration was
found in samples treated with Ag- and Ni-NPs, which had an
increase of 45.8 and 32.2 % with respect to the untreated
plants, while the lowest concentration was detected in plant
leaves exposed to SnO
(decrease of 55.1 %). Ca concentra-
tion in the stem was higher than the control for all treatments,
with a maximum increase of 54.4 % in plants treated with Co-
NPs and a minimum of 25.6 % in those exposed to SnO
The Ca content in root samples of different treatments can be
ranked as follows: Fe
>Ag= CeO
NPs; notably, plants exposed to Fe
-NPs showed an
increase of 69.8 % with respect to the control.
The NP treatment scarcely affected the content of K in
tomato above-ground organs (leaves and stem tissues) with
the exception of plants treated with SnO
-NPs, where a 50 %
decrease of K concentration was found in leaves with respect
to the untreated plants. On the other hand, K content in the
roots was deeply influenced by NPs in the following order:
Co> Ag>CeO
NPs; in plants
treated with Co- and Ag-NPs, K content was markedly higher
than the control (114.3 and 82.1 % higher, respectively)
whereas in plants exposed to Ni- and SnO
-NPs, the element
content was significantly lower (64.3 and 67.9 %, respec-
tively). Moreover, it should be noted that in some tomato
plants exposed to Ag-, Co- and Fe
-NPs, K content was
not detected in the leaves (data shown in Fig. 4and excluded
from the mean reported in Table 5) while a high K content was
stored in the roots.
Regarding Mg, its concentration in tomato above-ground
organs (leaves and stem) exhibited a behaviour similar to that
of Ca, showing the highest concentration in leaves, for sam-
ples treated with Ni- and Ag-NPs (increase of 54.2 and 37.5 %
whit respect to the control), the lowest for plants exposed to
-NPs (54.2 %) and showing a higher concentration in
stems for all treatments compared to the control. Mg content
in the roots of plants exposed to different treatments can be
ranked as follows: Co> Ag>CeO
NPs; in particular, plants exposed to Co-NPs showed a
marked increase (135.7 %) with respect to the untreated
plants, while both Ni- and SnO
-NPs decreased the element
content by 28.6 %.
Also with regard Na, as in Ca and Mg, Ag- and Ni-NP
treatments determined an increase of the element concentra-
tion in leaves with respect to the control (53.1 and 40.6 %,
respectively), whereas SnO
-NPs caused a marked decrease
(46.9 %). Moreover, Ag- and Co-NP treatments were asso-
ciated with an increase of Na in stems compared to the control
(53.1 and 40.6 %, respectively), while the lowest value was
found in plants exposed to CeO
-NPs. The Na content was
higher in roots of plants treated with Ag- and Co-NPs (97.6
Fig. 3 adESEM images of
nanoparticles detected in
root tissues of tomato of plants
grown in soil amended with
-NPs. e,fEDS spectra of
-NPs and natural
Environ Sci Pollut Res
and 57.1 %, respectively), whereas the lowest value was found
in plants exposed to Ni- and SnO
-NPs (69.0 and 73.8 %,
The average P content in leaves of different samples
can be ranked as follows: TiO
NPs; remarkably, only plants
treated with TiO
-NPs showed an increase of P
(15.4 %) with respect to the untreated plants. P concen-
tration in stems reached the highest value compared to
the control in plants treated with Ag-NPs (65.2 %),
while the lowest was found in plants exposed to Co-
NPs (8.7 %). Contrary to what was observed in leaves,
root tissues exposed to Ag-NPs showed an increase in P
concentration (55.6 %), whereas a notable decrease in
this element was detected in plants treated with Ni- and
-NPs (22.2 and 33.3 %, respectively).
Leaves of plants exposed to Ag-NPs showed an increase of
S content with respect to the control, whereas a marked
decrease was detected in plants treated with SnO
(60.7 %). As noted for Ca concentration in stems, S content
was higher with respect to the control for all treatments
with a maximum increase of 51.4 % in plants treated
with Co-NPs and a minimum of 9.9 % in those exposed
to CeO
-NPs. As already observed for K, Mg and Na,
roots of plants treated with Ag- and Co-NPs showed the
highest amount of S compared to the untreated plants
(21.4 and 25 %, respectively), while the lowest content
was determined in plants treated with Ni- and SnO
(42.9 and 50 %, respectively).
The nutrient concentration in tomato fruits showed a high
K content (from 59 % for CeO
-NPs to 63 % for
Ag-NPs) and a low level of Mg (from 33 % for SnO
-NPs to
47 % for CeO
-NPs), P (from ca. 51 % for Ag-,
Ni- and SnO
-NPs to ca. 63 % for Fe
-NPs) and S (from
ca. 41 % for Ag- and Ni-NPs and 53 % for CeO
NPs) after irrigation with NPs compared to the control
(Fig. 5), while no significant differences were found for Ca
and Na.
Tabl e 5 Nutrient concentration (g kg
d.w.) in tomato organs cultivated in control soil (Ctr) and soil polluted with NPs
Ca K Mg Na P S
Control 11.8 c 0.2 8.4 a 0.2 2.4 b 0.0 3.2 b 0.0 1.3 b 0.0 2.8 a 0.1
Ag-NPs 17.2 a 0.1 8.7 a 0.1 3.3 a 0.1 4.9 a 0.1 1.0 cd 0.0 3.2 a 0.1
Co-NPs 11.9 c 0.1 7.8 ab 0.1 2.7 b 0.0 3.4 b 0.0 0.9 d 0.0 1.9 b 0.0
Ni-NPs 15.6 b 0.3 8.5 a 0.1 3.7 a 0.1 4.5 a 0.1 1.1 c 0.0 2.8 a 0.0
-NPs 10.5 cd 0.2 7.5 b 0.0 2.2 b 0.0 2.9 c 0.0 1.2 c 0.0 1.8 b 0.0
-NPs 12.0 c 0.0 8.3 a 0.1 2.5 b 0.0 2.3 c 0.0 1.0 cd 0.0 1.91 b 0.0
-NPs 5.3 e 0.6 4.2 c 0.1 1.1 c 0.2 1.7 d 0.1 0.8 d 0.1 1.1 c 0.2
-NPs 10.7 cd 0.1 8.0 a 0.1 2.5 b 0.0 3.1 b 0.0 1.5 a 0.0 1.9 b 0.0
Control 19.5 c 0.2 8.7 a 0.1 3.1 b 0.0 2.2 b 0.0 2.3 c 0.0 11.1 c 0.1
Ag-NPs 28.5 ab 1.9 8.3 a 0.7 4.9 a 0.3 3.5 a 0.2 3.8 a 0.3 16.7 ab 0.9
Co-NPs 30.1 a 0.3 8.7 a 0.1 4.6 a 0.0 2.9 a 0.0 2.1 c 0.0 16.8 a 0.1
Ni-NPs 24.9 b 0.3 8.6 a 0.1 4.1 a 0.0 2.5 b 0.0 2.6 b 0.0 13.1 b 0.2
-NPs 25.1 b 0.5 8.8 a 0.0 3.9 a 0.1 1.9 c 0.0 2.4 bc 0.1 12.2 bc 0.5
-NPs 27.2 b 0.2 8.5 a 0.2 5.0 a 0.1 2.5 b 0.0 2.3 c 0.1 13.3 b 0.1
-NPs 24.5 b 0.1 8.5 a 0.0 4.2 a 0.0 2.1 b 0.0 2.9 ab 0.0 12.7 b 0.1
-NPs 26.1 b 0.2 8.7 a 0.1 4.1 a 0.1 2.1 b 0.0 2.6 b 0.0 13.2 b 0.3
Control 12.9 cd 0.3 2.8 d 0.1 1.4 d 0.1 4.2 cd 0.4 0.9 b 0.1 2.8 b 0.1
Ag-NPs 16.8 b 0.5 5.1 b 0.1 2.3 b 0.1 8.3 a 0.4 1.4 a 0.1 3.4 a 0.1
Co-NPs 14.6 c 0.1 6.0 a 0.0 3.3 a 0.1 6.6 b 0.0 1.5 a 0.0 3.5 a 0.0
Ni-NPs 12.0 d 0.1 1.0 e 0.0 1.0 e 0.1 1.3 e 0.0 0.7 c 0.0 1.6 c 0.0
-NPs 16.9 b 0.1 3.5 c 0.0 1.9 c 0.0 4.0 d 0.0 1.4 a 0.0 2.6 b 0.0
-NPs 21.9 a 0.3 2.7 d 0.0 2.0 c 0.0 4.3 d 0.1 1.2 ab 0.0 2.4 b 0.1
-NPs 10.8 d 0.3 0.9 e 0.0 1.0 e 0.1 1.1 e 0.0 0.6 c 0.0 1.4 c 0.0
-NPs 13.3 c 0.3 3.0 d 0.0 1.6 d 0.1 5.4 c 0.0 1.3 ab 0.0 2.6 b 0.0
Means followed by a different letter within a row are significantly different at P<0.05 according to one-way ANOVA Tukeystest
SD is the standard deviation
Environ Sci Pollut Res
We showed via ICP-OES that, with the exception of Fe
and TiO
-NPs, a high amount of NP component metal
remained in the rhizosphere soil and/or in the superficial
layers (03, 36 cm) of bulk soils as compared to the deeper
ones, thereby highlighting a low mobility of NPs in the soil.
Only in the case of TiO
-NPs, high values were found in the
deepest soil layers, suggesting a greater mobility of these
particles along the soil column than the other NPs
(Ben-Moshe et al. 2010).
Several studies to assess the bioavailability of NPs have
been conducted in hydroponic solution (Rico et al. 2011),
even though the growth medium is important since surface-
reactive particles, such as clays and organic matter-coated
particles in soil (Lee et al. 2012;Dimkpaetal.2012;Du
et al. 2011), can affect the behaviour of these NPs, favouring
their aggregation and thus decreasing the risk of toxicity
(Dinesh et al. 2012).
Chronic exposure to NPs affects plant growth, and in fact,
both the above- and below-ground growth was stunted in
tomato exposed to Ag-NPs, as already observed in Sorghum
bicolor and Triticum aestivum (Lee et al. 2012; Dimkpa et al.
2013), and atthe same time, we detected a significant increase
in the stem dry weight. This could be due to oxidative stress
and membrane damage, as observed in many
nanophytotoxicity studies (Wang et al. 2011), where the in-
crease of lignification observed in transgenic tobacco could be
Fig. 4 Concentration (mg g
major nutrients in some
specimens oftomato plants grown
in soil amended with Ag-, Co-,
and Fe
Fig. 5 Concentration (mg g
nutrients in tomato fruits of plants
grown in soil amended with metal
oxide and metallic NPs
Environ Sci Pollut Res
due to the over-expression of the peroxidase gene, thus en-
hancing the generation of H
(Kim et al. 2008). However, a
recent study conducted in rice plants showed that CeO
decreased the lignifications in rice despite the enhanced per-
oxidase activity and H
content (Rico et al. 2013a). In this
experiment, CeO
-NP treatment did not affect stem and root
elongation, while Wang et al. (2012) observed that the above-
ground part oftomato plants treated with CeO
-NPs showed a
slight improvement in the average height of stems. Moreover,
we observed that CeO
-NP treatment caused a decrease in the
dry biomass, as previously reported by Priester et al. (2012)in
soybean plants exposed to a comparable dose (0.1 g CeO
dry soil).
Magnetite promoted root growth for both elongation and
dry matter parameters since iron is one of the essential ele-
ments for plant growth, and thus, the application of nano-iron
on soybean leaf increased the dry pod weight, leaf plus dry
pod weight and yield (Sheykhbaglou et al. 2010). However,
these results contradict what Lee et al. (2010) reported: the
inhibition of root elongation on Arabidopsis thaliana after
exposure to Fe
-NPs in agar medium. Contrasting results
were also found for TiO
-NP treatment, which reduced the
leaf dry matter of tomatoes, whereas Song et al. (2013)re-
ported that the biomass of plants exposed to TiO
did not
significantly vary among 1,0005,000 mg L
These differences may be due to the fact that plants were
grown in agar medium and in Hoagland solution in the
studies by Lee et al. (2010) and Song et al. (2013), respec-
tively; indeed, differences in species and medium might ac-
count for our differing results (Anjum et al. 2013).
To the best of our knowledge, this is the first report about
the interaction between Co-, Ni- and SnO
-NPs and
L. esculentum. Our work showed that Co-, Ni- and SnO
treatments have a detrimental effect on plant growth, decreas-
ing the above-ground dry biomass compared to the control.
In this study, we employed ICP-OES to assess the compo-
nent metal concentration in tomato organs and ESEM to
investigate the amount of root-associated NPs that could be
available for translocation (Schwabe et al. 2013). A previous
study showed that roots could be the main route of the plants
exposure to NPs (Anjum et al. 2013). In this present study, for
all NPs, except Ni, an increase was found in the component
metal in the root tissues, which was accompanied by an
element translocation only for Ag (in stem, leaves and fruit),
Co (stem and leaves), Fe (fruit) and Ni (stem and fruit). While
no references were found regarding the impact of Co- and Ni-
NPs on plant species, generally, many studies on Ag-NPs
highlighted the possibility of silver transport in plant shoots
(Anjum et al. 2013). Moreover, Ag distribution in shoot
organs is species-specific and is correlated with the Ag-NP
size; in fact, smaller NPs showed a faster uptake in Populus
deltoides × nigra (Wang et al. 2013). Geisler-Lee et al. (2013)
found that Ag-NPs in A. thaliana are accumulated
progressively in roots in this sequence: border cells, root
cap, columella and columella initials.
Ce and Sn did not translocate in the control nor in the
treated tomato (CeO
- and SnO
-NPs, respectively). Sn con-
centration in soil and root tissues was lower than expected,
probably because of an underestimation of Sn concentration
due to the incomplete dissolution of SnO
-NPs in the acid
mixture used for the mineralization of soil and plants (Vittori
Antisari et al. 2013). As for CeO
-NPs, the literature is con-
tradictory. In research conducted on maize plants, cerium was
absent (Birbaum et al. 2010) or found at low concentrations in
the shoots of plants grown in low organic matter soil (Zhao
et al. 2012), while it was found in rice grains (Rico et al.
2013b). Moreover, a study conducted on tomato plants grown
in potting mix and treated with CeO
-NPs (1030 nm) at
increasing doses assessed the presence of Ce in the following
order: root>stem> leaf>fruit (Wang et al. 2012). These con-
trasting results could be due to the different size of the CeO
NPs employed, namely, 50105 nm in our experiment and
1030 nm in that of Wang et al. (2012). In fact, translocation
to the shoot is generally limited and depends on the NPs
primary diameter, as reported by Zhang et al. (2011).
The accumulation of iron in tomato roots growing in the
presence of magnetite was not accompanied by Fe transloca-
tion into shoots (Zhu et al. 2008; Trujillo-Reyes et al. 2014)
but only into the fruit, probably because the Fe was retained in
the roots similarly to low soluble compounds (e.g. oxides and
organic acids) (Taiz and Zeiger 1998).
In contrast to previously published data by Servin et al.
(2012,2013) regarding the TiO
-NP uptake by Cucumis
sativus, in our study, we did not assess Ti translocation in
the above-ground organs. However, the ESEM images
showed the presence of TiO
-NPs distributed parallel to the
longitudinal section of roots. Probably, the translocation of
NPs is associated with the absorption patterns of water and
nutrients within the root (Lee et al. 2010). Moreover, the
translocation of NPs, or of their component metal, could be
enhanced in crops with higher water transpiration (Schwabe
et al. 2013). According to Du et al. (2011), the impact of the
-NPs might result from the mere presence of the NPs in
cells or accumulated on the cell walls, which might cause
changes in the soil microenvironment.
To the best of our knowledge, studies have been carried out
on the impact of NPs on accumulation and translocation of
macro- and micro-elements in various plants, but not in toma-
to. However, the imbalance of nutrient absorption in the soil-
tomato system has been investigated (Carvalho Bertoli et al.
2012), and different physiological disorders related to heavy
metals pollution (Larbi et al. 2002; Dong et al. 2006)or
salinity (Carvajal et al. 2000; Levent Tuna et al. 2007)were
investigated. For example, Ca and K concentrations were seen
to decrease under stressful growing conditions determined by
salinity (Levent Tuna et al. 2007). Although the assessment of
Environ Sci Pollut Res
the impact of NPs on plant nutrient content is in its infancy, it
is highly possible that NPs could alter the nutrient profile of
food crops (Gardea-Torresdey et al. 2014).
Considering that accumulations of Ca, K, Mg, Na, P and S
in roots are significantly higher with respect to the control,
-, Co- and Fe
-NPs can be considered the NPs that
inhibit K translocation to stem and leaves. Moreover, CeO
NPs do not allow Ca, Mg and P to be translocated to leaves;
the same effect was exerted by Co-NPs on P and S and by
-NPs on Na, P and S. This evidence suggests a compet-
itive inhibition between K and other cations, e.g. NP compo-
nent metal, as highlighted in tomato stressed with high Cd
concentration by Carvalho Bertoli et al. (2012).
- and Ni-NPs determined a lower element concentra-
tion, linked to a lower plant growth (root and leaf dry biomass
significantly lower with respect to the control); however, no
data are available about this aspect; therefore, further investi-
gations are needed.
Tomato is a fruit generally rich in K and mineral salts, thus
representing an important source of elements for human be-
ings. In this study, the fruits of plants exposed to NPs showed
a general enrichment in K and depletion of Mg, P and S with
respect to the control.
Generally, little is known about the influence of NPs on
plant mineral composition or on the macro- and micro-
element accumulation in different plant organs. A study con-
ducted on Lactuca sativa exposed to nano-Fe/Fe
nano-Cu/CuO treatments showed changes in the nutrient up-
take. A decrease in Mg, P and Ca was detected in leaves of
plants exposed to Cu treatments, while an increase in S and Ca
was found in the roots (Trujillo-Reyes et al. 2014); probably,
the component metal damage to root cellular membranes
could alter the plants capacity to absorb and transport some
nutrients (Fernandes and Henriques 1991;Wangetal.2011).
The effect of TiO
-NPs on plant mineral composition in
cucumber fruits was investigated by Servin et al. (2013)who
found a significant increase in K and P in this organ and
suggest that NPs have an effect similar to that of plant hor-
mones like cytokinins and gibberellins (Mandeh et al. 2012),
given that cytokinins havebeen found to affect P and K uptake
(Wu et al. 2003). Moreover, a study on the quality of rice
grains from plants grown in soil amended with CeO
indicated that the exposure to these NPs could compromise
the quality of rice grain (Rico et al. 2013b).
The question of the uptake, bioaccumulation, biotransforma-
tion and risk of NPs in food crops is still not well understood.
The metal oxides and metallic engineered NPs were found to
have differing effects on the morphological parameters, the
uptake and the translocation of NP component metals in the
various tomato organs. The determination of the component
metal in tomato organs by ICP-OES showed that the tomato
plants exposed to Ag-, Co- and Ni-NPs have metal concen-
trations higher than the controls and that the fruits of plants
treated with Ag-NPs were contaminated. Moreover, changes
in the tomato plant mineral composition were assessed, and
the physiological disorders could be directly correlated to the
exposure to NPs. Further studies are required to evaluate the
behaviour ofthese NPs under field conditions and their fate in
the food chain.
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... The authors associate the results with the fact that the presence of TiO 2 in the sewage sludge might function as a promoter of plant nutrition. When the soil was amended with Fe 3 O 4 -NPs, root growth for both elongation and dry matter weight of tomato plant was promoted with a resultant increase in root Ca content of 69.8% (Vittori-Antisari et al., 2014). Similarly, the fruit K content of the same tomato plant from Fe 3 O 4 -NPs-amended soil increased by 59% (Vittori-Antisari et al., 2014). ...
... When the soil was amended with Fe 3 O 4 -NPs, root growth for both elongation and dry matter weight of tomato plant was promoted with a resultant increase in root Ca content of 69.8% (Vittori-Antisari et al., 2014). Similarly, the fruit K content of the same tomato plant from Fe 3 O 4 -NPs-amended soil increased by 59% (Vittori-Antisari et al., 2014). Root dipping of tomato plants into metal oxide NPs can also be a veritable strategy to improve the growth and development of tomato plants. ...
Engineered nanomaterials (ENMs) have found important usage in agriculture, especially in the areas of crop improvement and production, and disease control. Due to the special attributives possessed by ENMs especially their nano size and high surface reactivity, they are able to penetrate cells of plants and interact intracellularly with plant processes and metabolism. These interactions always lead to effects in plants that may be detrimental or promotional. In fact, studies have shown that ENMs can influence seed germination, alter plant physiology and metabolism and in general modify plant growth and development. In this chapter, we discussed the types of ENMs, their relevance to agriculture, and most importantly the beneficial effects of ENM application on some selected agricultural crops. Conclusively, ENMs can impact positive or negative impacts on plants’ growth and development, however, the paucity of information on the mechanisms behind the effects of ENMs calls for further studies.
... The translocation of TiO 2 in Cucumis sativus was also found, wherein Servin et al. [31,68] reported its translocation from root to fruit. In tomato, TiO 2 was however, not accumulated when it irrigated with TiO 2 NPs compared to control [69]. It is still unclear that Ti translocated in plant system is originated from NPs applied or from inherited soil system. ...
... Maximum leaf and root surface area of 16.6 cm 2 and 28 cm 2 respectively was reported when nano titanium was foliar sprayed on spot blotch infected plants. Similarly, the plant fresh and dry weight were also increased [69]. Similar improvement in plant growth character of barley in terms of number of ears was also reported [26]. ...
Nanotechnology is catching great interest globally due to its extended applications in various fields. Among different nanoparticles (NPs), titanium dioxide (TiO2) based NPs have been widely used thus becoming the important part of daily life of human-beings. TiO2 NPs have been used since long in agriculture sector in particular as heavy metal remediater, stress manager, plant growth promoter and quality enhancer agent. TiO2 NPs having physiological, biochemical and morphological effects on plant's metabolic activity due to increased making of biomass and is also a proficient source of nutrients. However, over application and leakage of TiO2 NPs proved toxic to plants, soil oraganims, animals and humans. Overall, it is obvious from this chapter that we need to optimize the useful concentration levels of TiO2 NPs for various applications. Critical understanding abount uptake, traslocation, accumulation and toxicity of TiO2 NPs are of great importance to reduce their environmental impact upon use.
... Furthermore, IONPs have been shown to induce root and shoot growth in various plants (El-Temsah and Joner, 2012). In seeds treated with IONPs, increased germination and physicochemical characteristics lead to increased proliferation and yield (Liu and Lu, 2016;Vittori Antisari et al., 2015). The IONPs can reduce the transport of Cd and other heavy metals in the soil (Sebastian et al., 2019). ...
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Current research reveals the positive role of iron oxide nanoparticles (IONPs) and selenium (Se) in extenuation of arsenic (As) induced toxicity in Cucumis melo. C. melo plants grown in As spiked soil (20 mg kg⁻¹ As) showed reduced growth, chlorophyll (Chl) content, photosynthetic rate, stomatal conductivity and transpiration. On the other hand, the alone applications of IONPs or Se improved growth and physiochemical parameters of C. melo plants. Additionally, exogenous application IONPs and Se synergistically improved the activity of antioxidative enzymes and glyoxalase system in C. melo plants. In addition, the collective treatment of IONPs and Se reduced As uptake, enhanced rate of photosynthesis and increased gas exchange attributes of C. melo plants under As stress. Interactive effect of IONPs and Se regulated reduced glutathione (GSH), oxidized glutathione (GSSG) and ascorbate (AsA) content in C. melo plants exposed to As-contaminated Soil. IONPs and Se treatment also regulated expression of respiratory burst oxidase homologue D (RBOHD) gene, chlorophyll synthase (CHLG) and protochlorophyllide oxidoreductase (POR). Therefore, the combined treatment of IONPs and Se may enhance the growth of crop plants by alleviating As stress.
... TEM combined with EDX detects and characterizes ENMs in complex media [175]. It is capable of measuring the concentration of silver in solutions and tracking its transport from soil to roots, leaves, and fruits [176]. Single-particle multi-element fingerprinting (spMEF) using inductively coupled plasma time-of-flight mass spectrometry (ICP-TOFMS) has been used to distinguish ENMs against natural nanomaterials in soil [177]. ...
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Silver nanoparticles (AgNPs) are one of the most popular engineered nanomaterials (ENMs) because of their anti-microbial properties. In wastewater treatment ecosystems, ENMs can be removed by plant uptake or adsorption on biofilms. However, AgNPs can inhibit the activities of plants and microorganisms. This review outlines the effects of AgNPs on nitrogen-cycling bacteria in constructed wetlands (CWs).. Environmental conditions like organic matter, pH, ionic strength of soil along with the size, concentration, surface coating, speciation, and aggregation of AgNPs influence the toxicity. Bacterial activity is hampered by disruption of the cell membrane and extracellular polymers, reactive oxygen species (ROS) imbalance, DNA damage, inhibition of gene expression, protein functions, and energy production. Compared to heterotrophic bacteria, generally, nitrifying bacteria are more sensitive to AgNPs. Bacterial inhibition leads to a significant decrease in community diversity and reduction in nitrogen removal efficiency (NRE). Recovery of NRE is correlated with the resistance and functional redundancy of the community. Exposure to sublethal AgNP concentrations can upregulate nitrogen-cycling genes. The hormetic response and bacterial resilience are more evident in communities with high diversity. Plants enrich the diversity of nitrogen-cycling bacteria in planted CWs in the presence of AgNPs. Compared to unplanted CWs, the planted wetlands are resistant to AgNPs and consequently exhibit a better NRE after long-term exposure. Future endeavors to analyze the influence of AgNPs should be preceded by a long-term assessment of the complex interactions in actual treatment systems that are often overlooked in studies using synthetic wastewater.
... Very low or zero toxicity was observed in all cases (Barrena et al., 2009). On the other hand, Fe 3 O 4 -NPs have been found to enhance the root growth of tomato while a decrease was observed when SnO 2 -NPs was used (Antisari et al., 2015). ...
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Nanotechnology is a research area that has experienced tremendous development given the enormous potential of nanoparticles (NPs) to influence almost all industries and conventional processes. NPs have been extensively used in agriculture to improve plant physiology, production, and nutritional values of plant-based products. The large surface area and small size are some of the desired attributes for NPs that can substantially ameliorate plants’ physiological processes, thereby improving crop production. Nevertheless, the results derived from such research have not always been positive as NPs have been shown, in some cases, to negatively affect plants due to their potentially toxic nature. These toxic effects depend upon the size, concentration, nature, zeta potential, and shape of nanoparticles, as well as the used plant species. The most common response of plants under NPs toxicity is the activation of antioxidant systems and the production of secondary metabolites. The mitigation of such NPs-induced stress highly varies depending on the amount of NPs applied to the plant growth stage and the environmental conditions. On the contrary, higher photosynthetic rates, higher chlorophyll, and proline content, improved homeostasis, hormonal balance, and nutrient assimilation are the favorable physiological changes after NPs applications. Alternatively, NPs do not always exhibit positive or negative impacts on plants, and no physiological influences are sometimes observed. Considering such diversity of responses after the use of NPs on plants, this review summarizes the progress made in nanotechnology on the influence of different NPs in plant physiology through the use of indexes like seed germination, root and shoot morphology, photosynthesis, and their impact when used as carriers of cell signaling molecules such as nitric oxide (NO). Understanding the intimate dynamics of nanoparticle toxicity in plants can prove to be fruitful for the development of areas like agronomy, horticulture, plant pathology, plant physiology, etc. That, in return, can assist to ensure agricultural sustainability. Similarly, this may also help to pave the way to combat the drastic climate change and satisfy growing food demands for the ever-increasing world population. Further studies on molecular and genetic levels can certainly broaden the current understanding of NPs-plant interactions and devise the respective mitigation strategies for environmental safety.
Pathogenic diseases that affect multiple crops generate a substantial loss of products. Conventional pesticides and fungicides are used to treat pathogens. Although they have proven to be effective, their chemical characteristics cause environmental and health issues, so new efforts in developing innovative strategies for plant protection must be made to abandon the use of such toxic chemicals. Nanotechnology has been proposed as a new tool in agriculture for crop quality improvement and plant protection. In this book chapter, the latest developments toward the use of nanomaterials like metal oxide nanoparticles and natural nanofibers for treating pathogenic diseases are discussed where they have shown to be an effective and low-cost strategy to protect high demand crops from disease-causing pathogens offering advantages like high selectivity and low dosages needed, these materials also offer the possibility of using them as nanofertilizers and nanoemulsions designed to improve crop development and product conservation.
The extensive application of titanium dioxide nanoparticles (TiO2NPs) in agro-industrial practices leads to their high accumulation in the environment or agricultural soils. However, their threshold and ecotoxicological impacts on plants are still poorly understood. In this study, the hormetic effects of TiO2NPs at a concentration range of 0–2500 mg/L on the growth, and biochemical and physiological behaviors of Nigella arvensis in a hydroponic system were examined for three weeks. The translocation of TiO2NPs in plant tissues was characterized through scanning and transmission electron microscopy (SEM and TEM). The bioaccumulation of total titanium (Ti) was quantified by inductively coupled plasma atomic emission spectroscopy (ICP-AES). Briefly, the elongation of roots and shoots and the total biomass growth were significantly promoted at 100 mg/L TiO2NPs. As the results indicated, TiO2NPs had a hormesis effect on the proline content, i.e., a stimulating effect at the low concentrations of 50 and 100 mg/L and an inhibiting effect in the highest concentration of 2500 mg/L. A biphasic dose-response was observed against TiO2NPs in shoot soluble sugar and protein contents. The inhibitory effects were detected at ≥1000 mg/L TiO2NPs, where the synthesis of chlorophylls and carotenoid was reduced. At 1000 mg/ L, TiO2NPs significantly promoted the cellular H2O2 generation, and increased the activities of antioxidant enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT). Furthermore, it enhanced the total antioxidant content (TAC), total iridoid content (TIC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. Overall, the study revealed the physiological and biochemical alterations in a medicinal plant affected by TiO2NPs, which can help to use these NPs beneficially by eliminating their harmful effects.
Nowadays consumers have constantly exposed to nanoparticles (NPs) ingestion. Although the impact of NPs on the human has been studied by many authors, they did not consider the influence of food matrix components on bioaccessibility of NPs. This fact has encouraged us to investigate the influence of different food components on NPs. The investigation has been carried out to assess the influence of main food components on the MNPs (metallic nanoparticles) fate during the in vitro gastrointestinal simulation. The experiments have been carried out with the single-particle inductively coupled plasma mass spectrometry (SP-ICP-MS) as a tool for quantitative and qualitative analysis and the scanning transmission electron microscopy (STEM) as a means of qualitative analysis. The influence of various food components on NPs has been confirmed and it may be concluded that the matrix has an impact on the size and form of NPs. The presence of food components significantly changes the behaviour of NPs during simulated gastrointestinal digestion. Possible explanations of the influence of main nutrient groups, i.e. lipids, protein, salts, saccharides and vitamins on NPs have been proposed.
Poultry is a rapidly growing sector of agriculture that produces meat and egg. Chicken meat and egg are the inexpensive sources of proteins and minerals. In addition to supplying food, poultry industry also offers employment to millions of people. It is a source of rural income and alleviates poverty. Poultry industry strives to produce high-quality meat and egg to meet the food security of the ever-expanding population. The conventional feed is supplemented with antibiotics and inorganic minerals. Antibiotics are used as prophylaxis to prevent birds from avian infectious diseases and contamination by foodborne pathogens. The emergence of multi-drug resistant pathogens and the presence of antibiotics in poultry and meat products restrict their use. Nano-materials has shown its potential to be an alternative of antibiotics and also is being exploited to improve the growth of birds and enrichment of meat and egg. Nano-materials as a feed additive at a very low concentration, aid in the efficient conversion of feed to biomass and products. The positive effect of nano-materials on the growth and performance of broilers and layers are determined by their physico-chemical properties. Further, the use of nano-sensors can also help in detecting avian pathogens at the early stage. Here, we review the potential of nanomaterials in the poultry industry to sustain good level of animal yield, low mortality rate, while preserving the environment and consumer health.
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Aim of this work was to assess the impact of the chronic exposure of CeO2 nanoparticles (NPs) (50 to 105 nm nominal size) on soil microbial biomass.To evaluate if the CeO2 NPs can affect the soil quality, they were mixed to an A1 and A2 horizon of Epileptic Cambisols at a concentrations of 100 ppm and incubated in lab for short and medium (7 and 60 days) times, at a constant temperature (25°C) and moisture (60% WHC).The preliminary results of the soil physicochemical analyses have showed an insolubility of the CeO2 NPs at short-term incubation in water, EDTA and aqua regia. The biological assays detect a storing of Ce-CeO2 in the microbial biomass at short time that decreases in the C amount. An increment of the basal respiration and a decrease in the amount of carbon soil microbial biomass determined a higher metabolic quotient (qCO2) than the control test, that identifies a stressful situation, most evident in the short term condition.Physical-chemical characterization of the CeO2 NPs and of the soil before and after the NPs addition, was carried out by means of Environmental Scanning Electron Microscope (ESEM) and an Energy Dispersive Spectroscopy (EDS). The investigations showed Ce-NPs and Ce-compounds in both- incubation-condition samples. The control soil showed the presence of cerium associated with other elements, like P, Nd, La, Th e Si. From literature, it appears that these elements identify Monazite-Ce/Nd minerals, whose chemical formulas are respectively (Ce, La, Nd, Th) PO4 and (Nd, Ce, La) (P, Si) O4. The presence of CeO2 NPs was clearly detected in soil and recognized by ESEM morphological observations coupled with EDS characterization. The NPs chemical composition appears unaltered, while the size can be modified by NPs aggregation and clustering.The results contribute to setting reference baseline values of cerium in soil and indicate an impact on the amount of carbon soil microbial biomass due to a higher metabolic quotient (qCO2) that can condition the soil fertility.
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The suburban area of Bologna city in southeast portion of Po Valley (Northern Italy) is characterized by high emission from industrial, urban, agriculture and traffic sources. The presence of an urban waste incinerator get inhabitants to require answers about impact of its emissions on the environmental quality related to human health. The concentrations of some pollutants (Ba, Cd, Co, Cr, Cu, Ni, Pb, V and Zn) were determined in topsoil, plants and mosses tissues sampled in sites selected according to different falling out conditions, due to the incinerator and other sources of atmospheric emissions that affect the monitoring area. No correlation was found between metal content and the distance of the incinerator plant. The pollution load index (PLI) calculated for soil and moss indicated a low environmental pollution, while highest values in sites downwind of incinerator and in craft area indicate a moderate pollution.
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Nanoparticles (NPs) types diffused into the environment are increasing, giving potential damages to terrestrial ecosystems. In this work we investigated the nanoparticles toxicity as pure metal and two oxides (Co, SnO2 and CeO2) on earthworm survival (Lumbricus rubellus). A concentration of 5000 mg kg-1 dry soil of each NP was compared to the chronic dose of 10 mg kg-1. The interaction between NPs and soil microbial biomass was also studied in 7 days lenght incubation. No mortality was observed at the end of the experiment, but high concentration of Co was found in the 5000 kg-1 dry soil treated. despite low solubility of all NPs (solid-liquid partition coefficient >2.8 log 1 kg-1 pure metal NPs were (Co) more soluble than metal oxides nanoparticles (SnO2 and CeO2).dd s
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Nanoparticles (NPs) are characterized by their small size (less than 100 nm) and large surface area, which confer specific physicochemical properties as strength, electrical, and optical features. NPs can be derived from natural or anthropic sources, such as engineered or unwanted/incidental NPs. The composition, dimension, and morphology of engineered NPs enable their use in a variety of areas, such as electronic, biomedical, pharmaceutical, cosmetic, energy, environmental, catalysis, and materials science. As nanotechnology is an innovative and scientific growth area with an exponential production, more information is needed concerning the impacts of these nanomaterials (NMs) in the environment and, particularly, in animals/humans health and in plants performance. So, research on NPs as emerging contaminants is therefore a new field in environmental health. This minireview describes, briefly, the NPs characterization and their occurrence in the environment stating air, water, and soil. Finally, particular emphasis is given to the interaction of NPs with plants at different levels: morphology, physiology, and genotoxicity. By analyzing this compiled information, it is evident that research on NPs phytotoxicity is in the beginning, and more comprehensive studies are needed not only on NPs cytotoxicity and genotoxicity but also on the best and the most reliable methods of assessing NPs toxicity.
This study was performed to determine the effect of nano-iron oxide on soybean yield and quality. Field experiment was designed based on randomized complete block design with three replications. Treatments were five levels of nano-iron oxide (0, 0.25, 0.5, 0.75 and 1 g l-1). Results showed that nano-iron oxide at the concentration of 0.75 g l-1 was increased leaf + pod dry weight and pod dry weight. The highest grain yield was observed with using 0.5 g l-1 nano-iron oxide that showed 48% increase in grain yield in comparison with control. Other measured traits were not affected by the iron nano- particles.
This standard is a revision of ASTM Standard D 2050-78, reapproved 1982, (see abstract 1979/1999) and provides a glossary of terms used in the zip fastener industry.
Engineered nanomaterials (ENMs) are released into the environment with unknown implications in the food chain. Recent findings demonstrate that ENMs may accumulate and/or increase concentrations of the component metal or carbon nanomaterials in the fruits/grains of agricultural crops, have detrimental or beneficial effects on the agronomic traits, yield, and productivity of plants, induce modifications in the nutritional value of food crops, and transfer within trophic levels. Given this information, important questions needed to be resolved include: a determination of actual or predicted concentrations of ENMs through the development of new and perhaps hybridized analytical tools, assessment of the nutritional content modifications and/or accumulation of ENMs, component metal, and co-contaminants in edible plants and their implications on human diet, nutrition, and health, assessment of the consequences of ENM-induced changes in soil health, physiological process, and yield on agricultural production and food security, and transfer of ENMs in trophic levels. Given the significant implications of ENMs exposure and the rather large knowledge gaps that exist, it will be prudent to observe judicious and targeted use of ENMs so as to minimize environmental release until a comprehensive environmental fate and effects assessment can be undertaken.
Cerium oxide nanoparticles (nCeO2) have been shown to have significant interactions in plants; however, there are limited reports on their impacts in rice (Oryza sativa). Given the widespread environmental dispersal of nCeO2, it is paramount to understand its biochemical and molecular impacts on a globally-important agricultural crop such as rice. This study was carried out to determine the impact of nCeO2 on the oxidative stress, membrane damage, antioxidant enzymes' activities, and macromolecular changes in the roots of rice seedlings. Rice seeds (medium amylose) were grown for 10 days in nCeO2 suspensions (0 to 500 mg l-1). Results showed that Ce in root seedlings increased as the external nCeO2 increased without visible signs of toxicity. Relative to the control, the 62.5 mg nCeO2 l-1 reduced the H2O2 generation in the roots by 75%. At 125 mg nCeO2 l-1, the roots showed enhanced lipid peroxidation and electrolyte leakage, while at 500 mg l-1, the nCeO2 increased the H2O2 generation in roots and reduced the fatty acid content. The lignin content decreased by 20% at 500 mg nCeO2 l-1, despite the parallel increase in H2O2 content and peroxidase activities. Synchrotron -XRF confirmed the presence of Ce in the vascular tissues of the roots.