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Adhesive and antimicrobial properties of Lactobacillus acidophilus strain Er 317/402 “Narine” for its use in some types of blood cancer
... To study the adhesion properties of bacterial cells, hemagglutination reaction with erythrocytes of different animals and humans is widely used: hemagglutination with at least one of those red blood cells indicates the presence of bacterial adhesion factors [24][25][26]. The assay with preparing erythrocytes in formalin was carried out as follows: an appropriate quantity of blood (human, sheep, bull) was defibrinized and filtrated as a final concentration of 8 % erythrocytes. ...
One of the main requirements for probiotics is their ability to survive during passage through gastrointestinal tract and to maintain their activity at different adverse conditions. The aim of the study was to look for the strains of lactobacilli and streptococci with high adhesive properties even affected by inhibitory substances, such as nitrates (NO3⁻). To study the adhesion properties hemagglutination reaction of bacterial cells with red blood cells of different animals and humans was used. The acid formation ability of bacteria was determined by the method of titration after 7 days of incubation in the sterile milk. These properties were investigated at different concentrations of NO3⁻. The high concentration (mostly ≥2.0 %) NO3⁻ inhibited the growth of both lactobacilli and streptococci, but compared with streptococcal cultures lactobacilli, especially Lactobacillus acidophilus Ep 317/402, have shown more stability and higher adhesive properties. In addition, the concentrations of NO3⁻ of 0.5–2.0 % decreased the acid-forming activity of the strains, but even under these conditions they coagulated milk and, in comparison to control, formed low acidity in milk. Thus, the L. acidophilus Ep 317/402 with high adhesive properties has demonstrated a higher activity of NO3⁻ transformation.
... To study the adhesion properties of bacterial cells, hemagglutination reaction with erythrocytes of different animals and humans is widely used: hemagglutination with at least one of those red blood cells indicates the presence of bacterial adhesion factors [24][25][26]. The assay with preparing erythrocytes in formalin was carried out as follows: an appropriate quantity of blood (human, sheep, bull) was defibrinized and filtrated as a final concentration of 8 % erythrocytes. ...
One of the main requirements for probiotics is their ability to survive during
passage through gastrointestinal tract and to maintain their activity at different adverse conditions. The aim of the study was to look for the strains of lactobacilli and streptococci with high adhesive properties even affected by inhibitory substances, such as nitrates (NO3−). To study the adhesion properties hemagglutination reaction of bacterial cells with red blood cells of different animals and humans was used. The acid formation ability of bacteria was determined by the method of titration after 7 days of incubation in the sterile milk. These properties were investigated at different concentrations of NO3−. The high concentration (mostly ≥2.0 %) NO3− inhibited the growth of both lactobacilli and streptococci, but compared with streptococcal cultures lactobacilli, especially Lactobacillus acidophilus Ep 317/402, have shown more stability and higher adhesive properties. In addition, the concentrations of NO3− of 0.5–2.0 % decreased the acid-forming activity of the strains, but even under these conditions they coagulated milk and, in comparison to control, formed low acidity in milk. Thus, the L. acidophilus Ep 317/402 with high adhesive properties has demonstrated a higher activity of NO3− transformation.
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