Effect of ascorbic acid on reduced glutathione level in arsenic-loaded isolated liver tissues of rat

Bangladesh Journal of Pharmacology (Impact Factor: 1.05). 01/2006; 1(2). DOI: 10.3329/bjp.v1i2.491
Source: DOAJ


Isolated liver tissues of rat were loaded with trivalent arsenic and were exposed in presence and absence of ascorbic acid. The amount of reduced glutathione (GSH) in normal liver tissue was 52.0  0.2 g/g protein. Addition of arsenic to the tissues reduced the amount of GSH to 11.5  0.3 g/g protein. But when the arsenic loaded liver tissues were incubated with ascorbic acid at the concentration of 20 g/ml, the amount of GSH was 14.2  0.1 g/g protein. There was 22.6% increase of GSH level which was statistically significant (p<0.001) when compared with arsenic alone. This study suggests that ascorbic acid increased the GSH level in arsenic-treated rat's liver.

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    • "There is no satisfactory treatment regimen for arsenicosis . Some experts had recommended the use of spirulina (Misbahuddin et al., 2006), corn (Chowdhury et al., 2009), spinach (Umar, 2007), vitamin E (Ahmad et al., 1998), selenium (Nasir et al., 2002), N. sativa oil (Basher et al., 2014), garlic (Misbahuddin et al., 2013), zinc (Kamaluddin and Misbahuddin, 2006), α-lipoic acid (Tabassum, 2006), or other antioxidants enriched with vitamins like A (Hall, 1946; Ahmad et al., 1998), C (Ahmad et al., 1998; Saha, 2006), and E (Ahmad et al., 1998), along with discontinuation of arsenic contaminated water in the treatment of arsenicosis. These require prolong duration of treatment and develop recurrence after discontinuation of drug. "
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    ABSTRACT: The role of probiotics supplementation for 12 weeks on the colony count of Escherichia coli in the stool of moderate palmar arsenical keratosis (14), arsenic exposed controls (15) and healthy volunteers (9) were studied. The mean colony counts of E. coli in the stool of arsenic exposed controls and patients were reduced to 7.3 x 10(11) CFU/g and 3.2 x 10(11) CFU/g respectively in comparison to healthy volunteers (11.1 x 10(11) CFU/g). After probiotics supplementation, the E. coli count was significantly increased in patients (p=0.000) and arsenic exposed controls (p=0.000) in comparison to healthy volunteers (p=0.027). Patients excreted less arsenic through stool and supplementation with probiotics showed increased excretion of arsenic only in patients The level of arsenic in nail of patients was more than the arsenic exposed controls and healthy controls. Probiotics supplementation did not show any significant change. This study suggests that arsenicosis reduced the stool colony count of E. coli, less excretion of arsenic and probiotics supplementation reversed the processes.
    Preview · Article · Mar 2014 · Bangladesh Journal of Pharmacology
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    • "The potential of dietary antioxidants to reduce the As burden in human by increasing its metabolism has drawn attention in recent years (Dey, 2002; McCall and Balz, 1999). Some investigators suggested that the effectiveness of retinol, β-carotene (Chung et al., 2006), ascorbic acid (Saha, 2005), selenium (Spallholz et al., 2004), tocopherol (Ramanathan et al., 2003), alphalipoic acid (Tabassum, 2006), spinach (Umar, 2007) for the reduction of body burden of chronic As toxicity. Spinach is a rich source of antioxidants and micronutrients and can increase the serum antioxidant capacity in humans (Cao et al., 1998). "
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    ABSTRACT: The present study was undertaken with a view to evaluate the efficacy of spinach against arsenic (As) induced toxicity in rats during the period between July to October 2008. Thirty six female Long Evans rats (age about 120days; average body weight at day 0 = 154.5g) were randomly divided into three equal groups (n=12) and marked as T 0, T 1 and T 2 groups. Rats of T 0 group were given normal feed and water and kept as control. Rats of T 1 and T 2 groups were given 5mg Sodium arsenite/kg body weight (BW) and 5mg Sodium arsenite/kg (BW) plus spinach extract 100 mg/kg body weight respectively daily for 30 days orally. Four rats from each group were sacrificed at 10 days interval in order to quantitatively determine the As content in liver, lungs and kidney by using Hydride Generation Atomic Absorption Spectrophotometer. Serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and serum creatinine were determined by Autoanalyser. No visible clinical sign were observed in any group of experimental rats except loss of body weight in the spinach treated group. Tissue (lung, liver and kidney) concentration of As was significantly (p<0.01) higher in T 1 group rats compared to that of T 0 and T 2 groups and the highest concentration of As was found in kidney followed by lung and liver in T 1 group rats. After 30 days of feeding, spinach significantly (p<0.01) decreased As from lung, liver and kidney. As intoxication significantly (p<0.01) increased SGOT values but insignificantly decrease SGPT values and spinach treatment improve these condition. There was no significant effect found in serum creatinine level. It can be concluded that feeding of spinach could reduce body burden of As in rats.
    Full-text · Article · Sep 2010 · Bangladesh Journal of Veterinary Medicine
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    • "But still there is no specific treatment. Some investigators suggest the effectiveness of retinol, β-carotene (Chung et al., 2006), zinc (Valko et al., 2005), ascorbic acid (Saha, 2006), selenium (Spallholz et al., 2003), tocopherol (Ramanathan et al., 2003), and spirulina (Misbahuddin et al., 2006). Most of these are antioxidants. "
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    ABSTRACT: The present study investigated whether the administration of the ethanol extract of water hyacinth (Eichhornia crassipes) ameliorates arsenic from arsenic-treated rats. To induce arsenic accumulation in different organs, arsenic trioxide was administered orally by gavage at a dose of 500 µg/rat/day for 7 days. In search of an effective therapeutic agent to counteract arsenic accumulation and arsenic-induced oxidative stress, different concentrations of ethanol extract of root of water hyacinth (100%, 75%, 50%, and 25%) were administered by intraperitoneal injection for last two days which significantly reduced the arsenic accumulation in liver, spleen, kidney, intestine, lungs and skin. Besides, it reduced the oxidative stress caused by arsenic, which was evident by decreased levels of malondialdehyde (MDA) in the above organs. Treatment with either extract 50% or with alpha-tocopherol was applied singly and in combination to find out result for comparison of effectiveness on arsenic removal. But the reduction of arsenic and MDA was not dose dependent and not parallel. Water hyacinth, at the dose of 50% extract was observed to be most effective.
    Preview · Article · Jan 2007 · Bangladesh Journal of Pharmacology
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