Increase in the lack of vitamin D in patients with atherosclerosis is risk factor for CVD health. This work, evaluate the impacts of vitamin D deficiency in cardiovascular disease patients as an important objective and determine the relationship between serum vitamin D with blood glucose, cholesterol (CH), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoprotein (VLDL), alkaline phosphate (ALP), alanine transaminase (ALT), aspartate transaminase (AST), albumin, total-protein (T-protein), total-bilirubin (T-bilirubin), blood urea, creatinine, calcium (Ca 2+), sodium (Na +), potassium (K +), phosphate (PO 4 3-), Troponin and CK-MB. In this study 102 subjects wereconducted (39 women and 63 men), they were undergoing elective urgent coronary angiography who had (diameter stenosis < %50) as a patients group, and 45 healthy subjects (20 women and 25 men) were conducted as a control group.The biochemical parameters of serum total 25(OH) D, Troponin-hs and CK-MB were measured via fully automated analyzer Cobas e 411 diagnostic kits (Roche/Hitachi Cobas) of German origin. The parameters of serum blood glucose, CH, TG, LDL, HDL, VLDL, creatinine and urea measured by using fully automated chemical analyzer of Cobas c 311 with diagnostic kits (Roche/Hitachi Cobas), of German origin. The parameters of electrolytes Ca 2+ , K + , Na + and PO 4 3-measured via fully automated 9180 Electrolyte Analyzer. Blood samples were taken from both patients and control group, who were aged < 40 years old. The results of this study revealed that the mean levels of total vitamin D are significantly lower in patients group with CVD rather than in control group. Whereas, the mean levels of blood glucose, CH, TG, LDL, VLDL, ALP, ALT, AST, T-bilirubin, blood urea and creatinine are significantly increased in patients group with CVD rather than control group.While, the mean levels of HDL, albumin and T-protein are significantly decreased in patients group with CVD when compared to control group. The results display that mean levels of Ca 2+ , PO 4 3-, K + and Na + are significantly decreased in patients group with CVD rather than control group. The mean levels of cardiac biomarker Troponin and CK-MB are significantly increased in patients group with CVD than those in control group. In a correlation analysis, the results display no significant correlations among total vitamin D with blood glucose, CH, TG, HDL and VLDL, ALP, ALT, AST, albumin, T-bilirubin, T-protein, blood urea, creatinine, PO 4 3-, Na + and Troponin. While, there are significant correlations between total vitamin D with LDL, Ca 2+ , K + and CK-MB. These findings reveal that serum vitamin D and serum blood glucose could be used as biomarkers for the diagnostic accuracy of CVD in men and women. These data demonstrate that serum CK-MB and serum Troponin are indeed potential biomarkers for CVD, due to the high levels of area under the curve (AUC).