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Screening of Artocarpus heterophyllus (Jack fruit) seeds for anti-diabetic activity by using α-amylase inhibition assay.
Abstract
Diabetes mellitus is one of the most common endocrine diseases and type II diabetes is the major form accounting for 90% of cases worldwide. The inhibition of carbohydrate hydrolyzing enzymes such as α-amylase can be an important strategy in the control of blood glucose levels in patients with type II diabetes. The extract of Artocarpus heterophyllus seeds were studied for the possible effects on the starch breakdown by α-amylase in vitro. Seed extract was partitioned by stepwise solvent extraction and action of several fractions (petroleum ether, chloroform and methanol) on salivary amylase activity as well as the indigenous α-amylase activity present in the aqueous seed extract was studied. The petroleum ether extract showed α-amylase inhibitory activity.
... In vitro antidiabetic activity of different plant extracts was determined by α-amylase inhibition. [11] The extracts of different plant samples were subjected to α-amylase inhibitory assay. The α-amylase inhibitory assay was performed using DNS (Sigma-Aldrich). ...
Objective: Treatment of diabetes without any adverse effects is a big challenge to the medical fraternity. There is need for alternative drugs with no side effects. The present study is aimed to develop a polyherbal formulation for effective management of diabetes using some of the indigenous plants taking lead from ethnobotanical information. Ajuga parviflora, Saraca asoca, Potentilla fulgens and Aconitum heterophyllum were selected for the development of antidiabetic formulation. Materials and Methods: Preliminary phytochemical screening of selected plants were performed. In-vitro antidiabetic activity of plants and standard drug acarbose was evaluated by α-amylase inhibition assay. The polyherbal suspension prepared and its antidiabetic activity was evaluated in a streptozotocin-induced diabetic rat model. Results: Preliminary phytochemical screening revealed the presence of alkaloids, terpenoids, flavonoids, phenolics and tannins in the plants. The prepared formulation was brown, easily pourable from the container and have redispersibility property with optimum particle size distribution. Sedimentation studies showed that the sedimentation volume of a formulation is in-between a range of 1. Administration of polyherbal formulation at 200 mg/kg and 400 mg/kg dose for 21 days to diabetic rats decreased fasting blood sugar 159 ± 2.81 mg/dL and 147.74 ± 2.03 mg/dL respectively as compared to the diabetic control group 361 ± 3.89 mg/dL. Conclusion: The present findings indicated that developed polyherbal formulation at a dose of 200 mg/kg and 400 mg/kg showed significant antihyperglycemic activity (***p<0.001) in streptozotocin induced diabetic rat model.
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