Micropropagation of rubber trees (Hevea brasiliensis Muell. Arg.)

ArticleinGenetics and Molecular Biology 21(3) · September 1998with115 Reads
DOI: 10.1590/S1415-47571998000300018 · Source: DOAJ
Abstract
Tissue cultures were established from newly expanded leaves and axillary buds of rubber trees (Hevea brasiliensis Muell. Arg.). Calli formed from these explants, but no regeneration occurred. Shoots were obtained from axillary buds cultured on Murashige and Skoog's (MS) medium (Physiol. Plant. 15: 473-497, 1962) supplemented with 1.0 mg/l kinetin, 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 20 g/l sucrose and 4 g/l Difco agar. Formation of a root similar to a tap root was induced on MS medium supplemented with 5.0 mg/l naphthaleneacetic acid (NAA), 3.0 mg/l indolylbutyric acid (IBA), 50 g/l sucrose and 4 g/l Difco agar. Several types of explants were used in attempts to recover complete rubber tree plants with well-developed tap roots. Leaf explants and axillary buds formed calli on MS basic medium with different combinations of kinetin, benzylaminopurine (BAP), 2,4-D, IBA, NAA and indolylacetic acid (IAA). The antibiotic tetracycline was also used to control possible bacterial infections. However, no antibiotic effect was noted. Calli formation was abundant, but no regeneration was observed when the calli from different media was transferred to MS medium without growth hormones. On this basic medium, callus cultures became necrotic and died. Shoots developed from axillary buds, rooted vigorously when cultured on MS medium with NAA, IAA, and IBA. Based on these results, further studies with commercially important clones should lead to a feasible micropropagation technique.
    • "Callogenesis was induced in 10 types of modified basal medium based on Woody Plant Media (WPM) (Minh & Thu 2001), Meristem Culture Media (MC) (Lineberger & Wanstreet 1983) and Murashige and Skoog media (1962) Indian modification enriched with zeatin (MS(ID)Z) (Table 1). MS medium used in this study was supplemented with a combination of growth regulators similar to that reported by Batista et al. (1998). Embryogenesis was promoted using modified RRIM Differentiation Media 1 (RD1) where RD1-C1 was RD1 supplemented with 0.2 mg/L NAA, 1 mg/L BA, 4 mg/L IBA, 0.5 mg/L zeatin and 7% sucrose, and RD1-E2 was RD1 supplemented with 1.5 mg/L BA, 1 mg/L IBA, 0.8 mg/L kinetin, and 7% sucrose. "
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