Micropropagation of rubber trees (Hevea brasiliensis Muell. Arg.)

ArticleinGenetics and Molecular Biology 21(3) · September 1998with115 Reads
DOI: 10.1590/S1415-47571998000300018 · Source: DOAJ
Tissue cultures were established from newly expanded leaves and axillary buds of rubber trees (Hevea brasiliensis Muell. Arg.). Calli formed from these explants, but no regeneration occurred. Shoots were obtained from axillary buds cultured on Murashige and Skoog's (MS) medium (Physiol. Plant. 15: 473-497, 1962) supplemented with 1.0 mg/l kinetin, 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 20 g/l sucrose and 4 g/l Difco agar. Formation of a root similar to a tap root was induced on MS medium supplemented with 5.0 mg/l naphthaleneacetic acid (NAA), 3.0 mg/l indolylbutyric acid (IBA), 50 g/l sucrose and 4 g/l Difco agar. Several types of explants were used in attempts to recover complete rubber tree plants with well-developed tap roots. Leaf explants and axillary buds formed calli on MS basic medium with different combinations of kinetin, benzylaminopurine (BAP), 2,4-D, IBA, NAA and indolylacetic acid (IAA). The antibiotic tetracycline was also used to control possible bacterial infections. However, no antibiotic effect was noted. Calli formation was abundant, but no regeneration was observed when the calli from different media was transferred to MS medium without growth hormones. On this basic medium, callus cultures became necrotic and died. Shoots developed from axillary buds, rooted vigorously when cultured on MS medium with NAA, IAA, and IBA. Based on these results, further studies with commercially important clones should lead to a feasible micropropagation technique.
    • "Callogenesis was induced in 10 types of modified basal medium based on Woody Plant Media (WPM) (Minh & Thu 2001), Meristem Culture Media (MC) (Lineberger & Wanstreet 1983) and Murashige and Skoog media (1962) Indian modification enriched with zeatin (MS(ID)Z) (Table 1). MS medium used in this study was supplemented with a combination of growth regulators similar to that reported by Batista et al. (1998). Embryogenesis was promoted using modified RRIM Differentiation Media 1 (RD1) where RD1-C1 was RD1 supplemented with 0.2 mg/L NAA, 1 mg/L BA, 4 mg/L IBA, 0.5 mg/L zeatin and 7% sucrose, and RD1-E2 was RD1 supplemented with 1.5 mg/L BA, 1 mg/L IBA, 0.8 mg/L kinetin, and 7% sucrose. "
    [Show abstract] [Hide abstract] ABSTRACT: Adventitious roots and shoot formation were induced from lateral meristem and shoot apical meristem of in vitro grown seedling of Hevea brasiliensis RRIM 2020 clone. Concurrently, shoots and roots were also induced for axillary buds (nodules) from matured bud stumps of this clone. The highest callogenesis at 93% was achieved from lateral meristem culture of RRIM 2020 initiated on Woody Plant media (WPM)-A which was WPM supplemented with 0.1 mg L-1 benzylaminopurine (BA), 0.5 mg L-1 2,4-dinitrophenylhydrazine (2,4-D) and 3% sucrose. The second highest callogenesis at 61% was obtained from lateral meristem culture in WPM-C which was WPM supplemented with 0.5 mg L-1 BA, 0.5 mg L-1 2,4-D, 0.5 mg/L zeatin, 10% coconut water (cw) and 7% sucrose, and this was followed by 49% callogenesis obtained from lateral meristem culture in Murashige and Skoog-Indian modification containing zeatin (MS(ID)Z). Embryogenesis induced in differentiation media, RD1 supplemented with kinetin or zeatin with a combination of plant growth hormones such as benzylaminopurine (BA), indolebutyric acid (IBA), 2,4-D and naphthaleneacetic acid (NAA) has successfully generated rooted embryoids. Rooting of lateral meristem culture was induced from calli initiated on WPM(C) containing 0.5 mg L-1 zeatin without casein hydrolysate. Meanwhile, the shoot apical meristem culture of RRIM 2020 initiated on developmental media, DM04 and a modified WPM (WPM(B)-M) has successfully enhanced shoot growth to 79% and 36% respectively. Apparently, adventitious shoots regeneration of shoot apical meristem was induced with two different meristem culture media containing thidiazuron (TDZ) (MC(A)-M and modified MC(B)-M). In both cases, while elongation and expansion of roots appeared to be retarded, further shoot development was triggered when the apical meristem was cultured on MS(ID)Z.
    Full-text · Article · Dec 2014 · PROPAGATION OF ORNAMENTAL PLANTS
  • [Show abstract] [Hide abstract] ABSTRACT: A reliable cryopreservation technique was developed for friable embryogenic callus lines of Hevea brasiliensis. The study showed that reducing the CaCl(2) concentration of the pre-culture medium from 9 mM to 1 or 0 mM CaCl(2) before cryopreservation promoted post-thaw callus growth, 1 mM being the optimum CaCl(2) concentration for embryo regeneration. Post-thaw callus proliferation decreased in line with the increase of plated callus weight. The effect of cryopreservation was assessed on 39 independent lines showing that cryopreservation did not affect embryogenic and plant regeneration for a majority of lines. The decrease in CaCl(2) concentration of the pre-culture medium led to a drop in callus calcium content indicating a direct link between the CaCl(2) concentration of the pre-culture medium and the endogenous calcium content of the calli. It also highlighted the implication of tissue calcium content in cryotolerance. Callus water status and the different ways by which calcium could prevent cryoinjury is also discussed.
    Article · Jun 2007
  • [Show abstract] [Hide abstract] ABSTRACT: Euphorbia pulcherrima. Methods to eliminate poinsettia mosaic virus (PnMV) and reinfection by different methods to reveal the 'nature' of the branching factor.. (1988). Protoplast culture in agarose media with particular emphasis to streaky culture lenses.. Sequence analysis and genome organisation of poinsettia mosaic virus (PnMV) reveal closer relationship to marafiviruses than to tymoviruses.. Genetic engineering of poinsettia with the aim of enhancing its resistance to poinsettia mosaic virus. Acta Horticul-turae, 722: 321-325. D'Onofrio C., Morini S. (2001). Effect of light quality on in vitro production of callus in explants of three poinset-tia cultivars.
    Article · Dec 2008