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The Physiological Activities of Ripe Fruit of Poncirus trifoliata
Abstract
Ripe fruits of Poncirus trifoliata were examined with a view to development of functional foods and physiological activities were assessed. The flavonoid compound of the sarcocarp extract (SC), at 20.39 mg/g, was the highest of all extracts studied, whereas that in fruit juice extract (FJ) was 18.72 mg/g. The total polyphenol content of pericarp ethanol (PE) and water (PW) extracts were 60.54 mg/g and 45.91 mg/g, respectively. The nitrite scavenging ability of PW (2.0 mg/mL) was 52.27% at pH 1.2. The tyrosinase inhibitory activity of PE (2.0 mg/mL) was 23.23%, but SW showed no such activity at any tested concentration. The electron donating abilities of PW, SC, and FJ were greater than 50% when tested at 0.5 mg/mL. Notably, the of PW was 147.73 /mL. Inhibition of xanthine oxidase by PW and SE (0.5 mg/mL) were more than 90%, whereas the of SC was 18.28 /mL. These results indicate that P. trifoliate ripe fruits may potentially serve as components of valuable new functional foods.
... The premature fruits of PT have diverse biological activities, such as antiinflammatory properties and anti-Helicobacter pylori activities, and can treat gastrointestinal disease (Kim et al. 2011;Yoon et al. 2012). In addition, PT contains approximately 17.90 À 60.54 mg/g flavonoids (Lee et al. 2010) and has a total polyphenolic content of 29.29 ± 2.60 mg GAE (gallic acid equivalent)/g DW (distilled water) (Woo et al. 2017). Another study showed that PT contains 50 chemical components, including hesperidin, naringin, and poncirin (Lee et al. 2005). ...
... In addition, Hong et al. (2018) reported that the PT methanol extract potentially has anti-bacterial and anti-oxidant activities against both gram-negative and gram-positive pathogens. PT contains rich sources of polyphenols, including flavonoids (Lee et al. 2010), and phytosterols, such as b-sitosterol (Jayaprakasha et al. 2007), which might inhibit and/or enhance several important rumen microbes. ...
The effects of different inclusion rates of Poncirus trifoliata (PT) extract on in vitro ruminal fermentation parameters were investigated. Six dose levels were referred to as follows: PT0, control (without PT); PT1, (3 mg/L); PT3, (9 mg/L); PT5, (15 mg/L); PT7, (21 mg/L); PT9, (27 mg/L), each incubated with 50 mL of buffered rumen fluid and 300 mg of timothy hay as a substrate for 72 h of incubation using an in vitro batch culture system. The in vitro dry matter digestibility (IVDMD) was significantly affected by the PT dose, except after 6 and 48 h of incubation. Total volatile fatty acids (VFAs) increased quadratically (12 h of incubation) within the range of PT1–PT7, whereas decreased at PT9 dose. Methane (CH4) production and the proportion of CH4 in the total gas produced decreased linearly after 12 h of incubation. The relative abundance of Ruminococcus albus and Ruminoccocus flavefaciens was greater at the doses PT1 than PT0, whereas that of Fibrobacter succinogenes was greater at PT0 than at PT9 after 24 h of incubation. Methanogenic archaea decreased linearly with increasing PT dose after 12 h of incubation. The abundance of ciliate-associated methanogens decreased linearly over 24 h of incubation under PT7 and PT9 treatments. These results suggest that the dose of PT can modulate ruminal fermentation to alter VFA concentration and reduce CH4 production by altering ruminal bacterial abundance.
• Highlights
• This study evaluated the dose-response effects of Poncirus trifoliata as a methane mitigation agent in ruminants.
• Inclusion of Poncirus trifoliate lowered in vitro dry matter digestibility and total volatile fatty acid concentration.
• Inclusion of Poncirus trifoliata extract reduced methane production after 12 h of incubation but did not maintained.
• Inclusion of Poncirus trifoliata extract reduced methanogenic archaea and ciliate-associated methanogens abundance
... Recently, many types of researches and developments have been made in the field of functional foods and functional cosmetics for superior functional materials derived from medicinal plant resources that have been proven to be effective [6,7]. Also, significant research is underway in terms of the efficient use of natural resources and contribution to the development of new materials [8]. ...
... 주름조개풀(Oplismenus undulatifolius)은 명들대 또는 털주름 풀이라 불리는 벼과(Gramineae)의 다년생 초본으로 산야의 숲 속에서 지천으로 퍼져서 자라며, 우리나라에서는 중부 이남에 자생하며 세계적으로 지중해 연안에서 중앙 아시아, 인도 북부 를 거쳐 극동에 널리 분포한다 (Lee 1999 ...
The phenolic contents which were extracted with water and 70% ethanol from O. undulatifolius were 7.7, 10.1 mg/g, respectively. The 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity of water and ethanol extracts were 78, 82% at 50 μg/mL phenolics, respectively. The 2,2′-Azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid) radical cation decolorization activity were 92, 76% at 100 μg/mL phenolics. Antioxidant protection factor in water and ethanol extracts at 200 μg/mL phenolics were 1.51 and 2.08 PF, respectively. Thiobarbituric acid reactive substance were 84% in water extracts and 99% in ethanol extracts at 50 μg/mL phenolics, respectively. The inhibition activity on α-Glucosidase was 44% in ethanol extracts at 200 μg/mL phenolics. The inhibition activity on α-amylase was 37-88% in water extracts at 50-200 μg/mL phenolics. The tyrosinase inhibition activity as whitening effect were 82% in ethanol extracts. The elastase inhibition activity were 4, 61% in water and ethanol extracts, respectively. The collagenase inhibition activity of anti-wrinkle effect showed an excellent wrinkle improvement effect as 39% in water extracts and 67% in ethanol extracts at 200 μg/mL phenolics, respectively. The hyaluronidase inhibition activity as anti-inflammation effect of ethanol extracts was confirmed to 46% of inhibition at 200 μg/mL phenolic. The astringent effect of water and ethanol extracts was confirmed to 13, 32% of effect at 200 μg/ mL phenolic, respectively.
This study was performed to compare the antioxidative activities of methanol extracts from Asparagus cochinchinensis with whole root (W-AC), flesh (F-AC), and root bark (B-AC). To evaluate the antioxidative properties of their methanol extracts, 1,1-diphenyl-2-picrylhydrazyl radical, nitrite, hydroxyl radical, 2,2'-azino-bis(3-ethylbenz thiazoline-6-sulfonate) radical scavenging activities, and contents of total flavonoid and polyphenol contents were measured. B-AC extract showed the highest antioxidative activity, whereas F-AC extract showed the lowest. For B-AC extract, caffeic acid was isolated by preparative high-performance liquid chromatography and confirmed by liquid chromatography-mass spectrometry and absorption spectroscopy, which showed 1.6% of total polyphenol contents among all methanol extracts. © 2016, Korean Society of Food Science and Nutrition. All rights reserved.
Various unripe peach (Prunus persicaL. Batsch) seeds, which were picked on late May, were subjected to 80% methanol extracts to investigate the antioxidant activities, whitening effects, and anti-wrinkle activities. The yield of 6 cultivars (Takinosawa Gold, Kawanakawase Hakuto, Madoka, Yumefuji, Nagasawa Hakuho, and Hong Bak) from unripe peaches were the highest on Yumefuji, followed by Madoka, Kawanakawase Hakuto, Takinosawa Gold, Hong Bak, and Nagasawa Hakuho (6.16%, 6.08%, 5.65%, 5.25%, 5.23%, and 5.20%). The content of the total polyphenol of seed extracts ranged from 18.33 to 27.08 mg/g. The antioxidant in vitroassays including the DPPH and ABTS radical scavenging activities were the highest with Hong Bak and Kawanakawase Hakuto. The tyrosinase inhibition activity greatly affected the Takinosawa Gold and Kawanakawase Hakuto. Compared to the elastase inhibition activity of 6 cultivars, Madoka was higher than others, while the lowest was Hong Bak. The significant correlation coefficient values were determined (more than p=0.05) between the yield, total polyphenol content, antioxidant activities, tyrosinase inhibition, and elastase inhibition activities. The results suggested that the total polyphenol content has a high positive value on the DPPH, ABTS radical scavenging activity, and tyrosinase inhibition activity. Also, the tyrosinase inhibition activity and elastase inhibition activity showed a significant correlation (r=+0.594). The ABTS inhibition activity and tyrosinase inhibition activity that is the most suitable character represented significant positive correlation (r=+0.846). This data suggested that the methanol extracts from these unripe peaches could be potential candidates for natural cosmetics.
This study was carried out to investigate the biological activities, including the total polyphenolic contents, antioxidant activities, and tyrosinase inhibition activities, of various cultivars of unripe peaches (Prunus persica L. Batsch). The results indicate that the total polyphenolic content (gallic acid equivalent mg/g) was the highest in Yumefuji among six-cultivars (Takinosawa Gold, Kawanakawase Hakuto, Madoka, Yumefuji, Nagasawa Hakuho, and Hong Bak) of unripe peaches, whereas it was the lowest in Madoka. Antioxidant effects of unripe peaches were determined in three different in vitrobioassays measuring DPPH radical scavenging, ABTS radical scavenging, and reducing power activity. In the results, antioxidant activity was also higher in Yumefuji than other cultivars. Tyrosinase inhibition activity ranged from 4.65% to 8.58%, and Hong Bak displayed the highest tyrosinase inhibition activity, although the difference was not significant. This study will provide information on antioxidant and tyrosinase inhibition activities for the development of natural compounds as functional cosmetics.
In this study, extracts from Caragana sinica flowers and leaves were tested for antioxidant and angiotensin converting enzyme inhibitory activities, along with xanthine oxidase, tyrosinase, elastase, and astringent effects. Total phenolic compounds of acetone extracts from Caragana sinica flowers and leaves were the highest at 3.42 and 2.98 mg/g, respectively, when various extraction solvents were used. Optimal conditions for extraction of phenolic compounds from Caragana sinica leaves and flowers were 70% ethanol for 18 hr. DPPH scavenging activities were the highest in 70% ethanol extracts of Caragana sinica. ABTS radical cation decolorization values of 70% ethanol extracts were higher than those 60% ethanol extracts at 74%. Antioxidant protection factor was 1.2 PF in 70% ethanol extracts from Caragana sinica flowers and leaves. TBARS was lower than that of control (0.54 μM) in all sections. Angiotensin converting enzyme inhibitory activity of Caragana sinica flower extract was 80~90% at a phenolic concentration of 0.2~1.0 mg/mL, whereas xanthin oxidase inhibitory activity of Caragana sinica leaf extract was higher than that of flower extract. Tyrosinase inhibitory activity, which is related to skin-whitening, was above 20%, whereas elastase inhibitory activity related to anti-wrinkle effect was above 50% at a phenolic concentration of 0.8 mg/mL. Astringent effects of Caragana sinica flower and leaf extracts were higher than tannic acid as a control at an equivalent concentration. This result suggests that extracts from Caragana sinica flowers and leaves are suitable as functional foods having anti-hypertension, anti-gout, and medicinal cosmetic activities, including whitening and anti-wrinkle effects.
The biological activities of Smilax china L. rhizome (SCR), hot water (SCRW) and 70% ethanol extract (SCRE) were analyzed. The total phenolic contents of SCRW and SCRE were 51.7 and 100.5 mg/g, respectively. The measured flavonoid content of SCRW () was almost double that of SCRE (). SCRE () exhibited stronger antioxidant activity in the DPPH system than the positive control -tocopherol () or butylated hydroxy anisole () did. SCRE () also showed stronger ABTS radical scavenging activity, as did -tocopherol (). The SOD-like activity and Tyrosinase inhibition activity of SCRW and SCRE showed almost the same pattern. The best SOD-like activity and tyrosinase inhibition activity were measured as 24.9% and 20.3% in SCRW at , respectively. The cytotoxic effects of the SCR extracts were analyzed via MTT assay on human cancer and normal cells. SCRW and SCRE did not show cytotoxicity up to the concentration of against the normal human cell line HEK293. Against human breast cancer cells (MCF-7), SCRW inhibited MCF-7 growth (by 27.6%) better than the anticancer drug cyclophosphamide (15.5%) at . SCRE () inhibited the growth of human lung cancer cells A549 (37.6%) and human stomach cancer cells AGS (53.6%) more effective than did SCRW (21.0% and 35.4%) or CPA (22.2% and 31.7%). These results suggest the potential use of SCRE and SCRW as an excellent antioxidant and antiproliferative substance, respectively.
Antioxidant activities of water extracts of 20 medicinal plants (1 mg/mL) on peroxidation of linolic acid were evaluated by thiocyanate method, among which 11 showed strong antioxidant activity (> 70%). Higher hydroxy radical scavenging activity (> 60%) were shown in Corner officinalis, Acanthopanax sessiliflorus, and Epimedium koreanum than the other plants. Epimedium koreanum than the other plants extract showed highest superoxide radical scavenging activity (42%). Total polyphenol contents ranged from . Direct correlation between the antioxidant activity and polyphenol content (r=0.8) was established through simple regression analysis. for selected four plant extracts, showing highest polyphenol contents and antioxidant activities, were significantly higher than positive control. Total antioxidant activity of vitamin c was significantly lower than those of Acanthopanax sessiliflorus, Epimedium koreanum, and Erythrina variegata. Superoxide radical scavenging activity of Acanthopanax sessiliflorus was similar to BHA. Results suggest water extracts of some medicinal plants could be potential candidates for natural antioxidants.
Herbs have been utilized to treat acute and chronic disorders for thousands of years. The scientific basis for such utilization, however, has been poorly understood. Nonetheless, in the past few decades, significant advances in experimental methodology and molecular biology enabled researchers to investigate the potential use of phytochemicals to treat or manage a plethora of chronic diseases including various types of cancer, inflammatory diseases and cardiovascular abnormalities. This review summarizes some of the molecular mechanisms through which several phytochemicals may inhibit inflammation and cancer. In addition, the review discusses basic definitions, ethical and clinical aspects and some guidelines for the use of phytochemicals as chemopreventive or therapeutic agents.
The aerobic oxidation of xanthine by rat liver supernatant was greatly stimulated by the addition of methylene blue or of
NAD+: the latter was reduced during the reaction. Storage of the supernatant at -20° brought about an enhancement of the xanthine
oxidation rate measured without addition of cofactors. A similar "activation" was caused by prior incubation at 37° of the
unfractionated liver homogenate, or of the supernatant separated after sonic disruption of the homogenate. The same effect
was obtained by treatment with solvents, or by prior incubation at 37° of the supernatant in the presence of proteolytic enzymes
or under anaerobic conditions. The presence of xanthine accelerated the effect of proteolytic enzymes and of anaerobiosis.
Only the changes caused by anaerobiosis could be reversed by incubating the supernatant in air before the assay.
The reaction rate was apparently unaffected by these treatments if activity of the enzyme was measured in the presence of
methylene blue or of NAD+. The latter, however, was not reduced during the oxidation of xanthine by "activated" supernatants stored at -20° if the
reaction was run in the presence of oxygen. If the reaction was in anaerobiosis, uric acid and a corresponding amount of NADH
were formed by fresh, supernatant, and by supernatants activated at -20° or by prior incubation in anaerobiosis, but not by
supernatant activated by trypsin.
The hypothesis is formulated that most of the xanthine oxidase of rat liver supernatant is a dehydrogenase (Type D), and may
be converted (activated) into an oxidase (Type O).
To study the association between nitrite and nitrosamine intake and gastric cancer (GC), between meat and processed meat intake, GC and oesophageal cancer (OC), and between preserved fish, vegetable and smoked food intake and GC.
In this article we reviewed all the published cohort and case-control studies from 1985-2005, and analyzed the relationship between nitrosamine and nitrite intake and the most important related food intake (meat and processed meat, preserved vegetables and fish, smoked foods and beer drinking) and GC or OC risk. Sixty-one studies, 11 cohorts and 50 case-control studies were included.
Evidence from case-control studies supported an association between nitrite and nitrosamine intake with GC but evidence was insufficient in relation to OC. A high proportion of case-control studies found a positive association with meat intake for both tumours (11 of 16 studies on GC and 11 of 18 studies on OC). A relatively large number of case-control studies showed quite consistent results supporting a positive association between processed meat intake and GC and OC risk (10 of 14 studies on GC and 8 of 9 studies on OC). Almost all the case-control studies found a positive and significant association between preserved fish, vegetable and smoked food intake and GC. The evidence regarding OC was more limited. Overall the evidence from cohort studies was insufficient or more inconsistent than that from case-control studies.
The available evidence supports a positive association between nitrite and nitrosamine intake and GC, between meat and processed meat intake and GC and OC, and between preserved fish, vegetable and smoked food intake and GC, but is not conclusive.
We have tested tyrosinase inhibitory activity on the methanol extracts of 23 species marine algae and 23 species traditional medicinal plant. Among them, four medicinal plants, Ephedra sinica, Atractylodes japonica, Pinnelia ternata, and Citrus aurantium, showed strong inhibition potency over 90% at concentration of 0.33 mg/mL. Also, two marine algae. Enteromorpha compressa and Sargassum singgildianum showed mild inhibition potency over 50% at concentration of 0.33 mg/mL.
To develop an whitening cosmetics, we isolated the melanogenesis inhibitors from the unripe fruits of Poncirus trifoliata (Rutaceae). Isolated compounds were identified as poncirin(1), naringin(2), bis(2-methylheptyl)phthalate(3), avenalumic acid methyl ester(4) by comparison of physical and spectral data with those reported in the literature. Among the isolated compounds, bis(2-methylheptyl)phthalate showed most potent inhibitory effect on the melanogenesis in cultured B-16 mouse melanoma cell lines (IC50, 36.8 μM) compared with kojic acid (IC50, 150 μM).
The degradation of nitrite and the inhibition towards formation of carcinogenic nitrosamines by melanoidins, produced from the glucose-glycine system were investigated at various conditions. The degradation of nitrite was highest at pH 1.2 (29%), when the ratio of melanoidins to nitrite was 1: 3. The inhibition towards formation of nitrosamines by melanoidins had the same tendency as the degradation of nitrite, the inhibition also being highest at pH 1.2 (99%). In addition, melanoidins after nitrite treatment exhibited a little higher mutagenicity and much stronger desmutagenicity than those of the original melanoidins. The change of the structure of melanoidins after treating with nitrite was also investigated by HPLC and CP-MAS NMR.
The chemical properties and nitrite scavenging ability were analyzed which on a citron (Citrus junos) of 4 kinds purchased in different regions [Namhae (I, II), Tongyoung and Koheung] in Korea. Four kinds of organic acids were determined. Citric acid contents in flesh and peel of citron were . The organic acid content was the highest in citron purchased from Koheung. Total mineral content in citron was in a range of mg/100 g, the potassium content was the highest in the range of . The major free amino acid from citron were proline, asparagine and glutamic acid. And the highest in peel of citron purchased from Tongyoung by 326.9 mg/100 g. However, the kinds and contents of chemical components in citron were somewhat different among various regions. The electron donating ability using DPPH method of citron juice was more than at the concentration of and it was stronger by increased the juice concentration in the reaction mixture. SOD-like activity showed at the concentration of . The nitrite scavenging ability was pH and sample concentration dependent. It was the highest at the pH 1.2 and more than in citron juice. On the contrary in all concentration of added citron juice lower than at pH 4.2.
Antioxidative and antimicrobial activities were carried out on the Citrus Unshju peel solvent extracts in order to discover new functional activities. The amounts of polyphenol in 70% metanol extract (MtEx) was measured as 836.8 mg% in Citrus Unshju peel. The EDA (electron donating ability) of 0.01, 0.02 and 0.1% MtEx in Citrus Unshju peel were measured as levels of 81.3, 86.0 and 89.6%. The nitrite scavenging effects of Citrus peel were also determined as the levels of 34.4% (pH 1.2) and 19.5% (pH 7.0). The pH of react solution was more acidic, the nitrite scavenging effect was more increased. The order of antioxidatives was shown as TBHQ > BHT > TOC > ChEx > EaEx > EtEx > WaEx > Control in corn germ oil and TBHQ > ChEx > EaEx > BHT > EtEx > WaEx > TOC > Control in canola oil. A number of the extracts were certified to have antimicrobial activities for a small number of micro-organisms, similar gram negative and positive micro-organisms. According to the results above, it was summerized that Citrus Unshju peel had the higher total polyphenol, EDA, nitrite scavenging effects and antimicrobial activities. Also isolated extract from ChEx and EaEx had high antioxidative, these effects were very similar to that of -tocopherol and BHT. It would be proposed that Citrus peel can become a new natural source for antioxidative agents in future food industry.
Antioxidant activities of methanol extracts from sarcocarp and seed of Zizyphus jujuba var. inermis Rehder were investigated in vitro. Contents of total polyphenols in methanol extracts from sarcocarp and seed were 98.83, , respectively. Radical-scavenging activities of methanol extracts were examined using and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals, and hydrogen peroxide assay. Inhibition effects of methanol extracts on peroxidation of linoleic acid were examined by ferric thiocyanate and thiobarbituric acid methods. Both sarcocarp and seed of Zizyphus jujuba var. inermis Rehder showed relatively high antioxidant activities in various systems.
Chemical components in the peel and flesh of full riped trifoliate oranges(Poncirus trifoliata) were investigated. Contents of crude protein, crude fat and ash in peel and flesh were 5.15 and 3.31, 3.82 and 6.65, 2.62 and 2.09, respectively. Vitamin C contents were 4.70 mg in peel and 70.93 mg in flesh. Free sugars were fructose, glucose and sucrose, the level of each or total free sugars was twice higher in peel than in flesh. Organic acids were citric acid, malic acid and oxalic acid, the total contents in peel and flesh were 0.35 and 1.25, respectively. Free amino acids were aspartic acid, histidine, tyrosine, arginine, valine, lysine, ammonia, cysteine, alanine, glutamic acid, isoleucine, leucine in peel, and lysine, valine, ammonia, arginine, tyrosine, isoleucine, methionine, leucine, histidine, phenylalanine in flesh.
Lipid-lowering and antioxidant effects of Poncirus trifoliata extract were investigated in hyperlipidemic rat. Concentrations of FFA and triglyceride in plasma were decreased in Poncirus trifoliata extract treatment groups. Plasma total cholesterol and LDL-cholesterol were lower in Poncirus trifoliata extract treatment groups. However HDL-cholesterol showed no significant difference in all treatment groups. Concentrations of liver total cholesterol and triglyceride were decreased in Poncirus trifoliata extract treatment groups. Concentrations of plasma and liver TBARS showed a low values in Poncirus trifoliata extract treatment groups. The values of GSH-Px and CAT activities were increased in the Poncirus trifoliata extract treatment groups. However the values of SOD activity showed no significant difference in all treatment groups.
Methanol, ether, ethylacetate and water extracts from flesh and peel of yuna (Citrus junos) were investigated to analyze their ability of electron donating, nitrite scavenging and nitrosodimethylamine (NDMA) formation in model system. The electron donating ability of ynza flesh extract, when it added at 10 mg/ml or over, were more an , except ether extract. The high electron donating ability () was observed in the methanol extract and its effect was similar to BHA and ascorbic acid, when 40 mg/ml of methanol extract was added in reaction solution. Nitrite scavenging ability of all extracts ken yuza was increased in proportion to sample concentration and more than when 1 mg/ml sample was added, under pH 1.2. Also nitrite scavenging ability was higher in peel extracts than flesh extracts of yuza. Inhibition ratio of NDMA formation from flesh methanol extract of yuza was , when 40 mg/ml added under pH 1.2. In all samples, inhibition effects were lower than , at pH 4.2 and 6.0.
This study was carried out to investigate the effects of Salicornia herbacea L. on baking properties of white breads. Crude fiber and crude ash contents of Salicornia herbacea L. powder were 70.02% and 25.6%, respectively. Fe and Ca contents of Salicornia herbacea L. were 176 ppm and 1,175 ppm, respectively. The pH of dough with Salicornia herbacea L. powder was similar to that of control. Salinity contents did not show any significantly differences between bread added Salicornia herbacea L. powder and control. L (lightness) and b (yellowness) value of the dough were decreased by the addition of Salicornia herbacea L., but a (redness) value was increased. The volume of dough during fermentation and the baking loss did not show significant difference between treatments and control. The weight of bread was increased by the addition of Salicornia herbacea L. but the volume was not.
Oleuropein and hydroxytyrosol, two phenolic compounds contained in olives and olive oil, are known to possess several biological properties, many of which may be related, partially at least, to their antioxidant and free radical-scavenger ability. Hence, together with their scavenging activity against the stable 1,1-diphenyl-2-picrylhydrazyl radical (DPPH test), we have investigated the antioxidative effect of oleuropein and hydroxytyrosol in a model system consisting of dipalmitoylphosphatidylcholine/linoleic acid unilamellar vesicles (DPPC/LA LUVs) and a water-soluble azo compound as a free radical generator (LP–LUV test). The results obtained were also interpreted in the light of biophenol interactions, studied by differential scanning calorimetry (DSC), with dimyristoylphosphatidylcholine (DMPC) vesicles as a biological membrane model. Our results obtained in the DPPH and LP–LUV tests confirm the good scavenger activity and antioxidant effect of oleuropein and hydroxytyrosol. However, while both compounds exhibit comparable effectiveness in the DPPH test (hydroxytyrosol being slightly more active than oleuropein), oleuropein seems, in the LP–LUV test, a better antioxidant than hydroxytyrosol. Besides oleuropein shows a better antioxidant activity in the membranous system than in homogenous solution. Furthermore, oleuropein, but not hydroxytyrosol, interacts with DMPC vesicles, causing shifts, toward lower values, of the calorimetric peak temperature (Tm), associated to the gel to liquid-crystal phase transition, typical for DMPC multilayers. The hypothesis will be discussed that hydroxytyrosol can serve as scavenger of aqueous peroxyl radicals near the membrane surface, while oleuropein acts also as a scavenger of chain-propagating lipid peroxyl radicals within the membranes.
METHODS for measuring antioxidants and appraising antioxidant activity appear to be of two general types. If the chemical nature of the antioxidant is known, one may strive for a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups. Alternatively one may observe the inhibition of some natural oxidative process such as the β-oxidation of fats, as a function of the added antioxidant.
The fruit ofPoncirus trifoliata shows glandular cell complexes in the exocarp, which produce a volatile oil rich in monoterpenes but poor in sesquiterpenes and oxigenated compounds. The juice vesicles of the endocarp possess similar cell complexes mainly containing sesquiterpenes and oxigenated compounds, whereas monoterpenes only occur in small amounts. By the use of combined gas chromatography-mass spectrometry 19 components of the rind oil and 15 compounds of the endocarp oil could be identified.
As demonstrated by electron microscopy the terpenes most probably are synthesized predominantly, if not exclusively in plastids. As shown by gasradiochromatography radioactive precursors (14CO2 and14C-leucine) are incorporated into mono- and sesqui-terpenes to a different extent.
This is due to two gland types producing essential oils of different composition with regard to their mono- and sesqui-terpene percentage. In fruit development the exocarp glands differentiate earlier than the endocarp glands do. The activity of exogenously applied14CO2 first reaches the peripheral glands and later on appears in the interior glands. Depending upon the growth season, labelled leucine transported by the conducting tissues from lower plant parts leads to a high specific activity of the sesqui-terpenes and oxigenated compounds. It could be argued that in this instance the glands of the pulp are better provided with precursors than the exocarp glands. The successive maxima of essential oil production in both glandular complexes, and the changes in the concentration of individual oil constituents during the ontogeny of the fruit also contribute to different incorporation ratios of radioactive precursors into mono- and sesqui-terpenes.
Propolis is extensively used in Argentine folk medicine. Alcoholic extracts of propolis from different regions of Argentina were prepared. The extracts were analysed for the determination of total flavonoid content (from 13.3 to 42.6 mg/g of propolis) by using the aluminum nitrate method, UV spectrophotometry and thin layer chromatography. All of them contained high total flavonoid content. It was also observed that all samples of ethanolic extracts of propolis showed free radical-scavenging activity in terms of scavenging of the radical DPPH but the highest activities were found for samples from Tucumán and Santiago del Estero. In all cases with 20 μg/ml of soluble principles, the percentage of DPPH degradation was different (Banda Oeste: 67.5%; Verónica: 45%; Forres: 35%; Saenz Peña: 20% and Juan José Castelli: 55%). These results may justify their use as a source of natural antioxidants.
The detergent-induced amplification of lucigenin-dependent chemiluminescence of O2-, generated by xanthine oxidase or microsomal NADPH oxidase was studied. An assay system is described which is at least 10 times more sensitive than normal lucigenin-dependent chemiluminescence due to the amplification by high concentrations of octylphenylpolyethylene glycol (Triton X-100). Compared to the superoxide dismutase-sensitive reduction of acetylated cytochrome c, a 3750-fold lower amount of microsomal protein was necessary to produce an O2- signal 10-fold above the background. In contrast to cytochrome c reduction, detergent-amplified chemiluminescence of lucigenin was completely inhibited by superoxide dismutase and therefore more selective for O2-. The membrane-bound and Triton X-100-solubilized NADPH oxidase from microsomes of macrophages was activated by ethylene glycol bis(beta-aminoethyl ether)-N,N'-tetraacetic acid and inhibited by Ca2+ and sodium dodecyl sulfate. The membrane-bound enzyme showed a Km value of 1.35 microM, which decreased to 0.95 microM after the addition of 12% (g/g) Triton X-100. The Km and Vmax values of soluble xanthine oxidase were not influenced by Triton X-100, indicating that the enzyme activities were not impaired by the high concentrations of detergent.
Recent work suggests that oxygen radicals may be important mediators of damage in a wide variety of pathologic conditions. In this review we consider the evidence supporting the participation of oxygen radicals in the adult respiratory distress syndrome, in ischemia reperfusion injury in the myocardium, and in cerebral vascular injury in acute hypertension and traumatic brain injury. In the adult respiratory distress syndrome there is active sequestration of polymorphonuclear neutrophils in the pulmonary vascular system. There is evidence that activation of these neutrophils results in the production of oxygen radicals which injure the capillary membrane and increase permeability, leading to progressive hypoxia and decreased lung compliance which are hallmarks of the syndrome. In acute arterial hypertension or experimental brain injury oxygen radicals are important mediators of vascular damage. The metabolism of arachidonic acid is the source of oxygen free radical production in these conditions. In myocardial ischemia and reperfusion injury, the ischemic myocyte is "primed" for free radical production. With reperfusion and reintroduction of molecular oxygen there is a burst of oxygen radical production resulting in extensive tissue destruction. Myocardial ischemia--reperfusion injury shares in common with the other two syndromes activation of the arachidonic acid cascade and acute inflammation. Thus it would appear that the generation of toxic oxygen species may represent a final common pathway of tissue destruction in several pathophysiologic states.
1. Tyrosinase is a copper-containing enzyme responsible for the production of melanin pigment throughout the phylogenetic spectrum. 2. In mammals, tyrosinase is a glycosylated enzyme found specifically in melanocytes--cells functional in the production and secretion of pigment granules. 3. Although many factors determine the type, quantity and quality of the melanin produced, tyrosinase activity is the critical factor that ultimately regulates melanogenesis.
Angelicin, a coumarin isolated from the aerial parts of Heracleum thomsoni, showed nonspecific spasmolytic activity in a variety of in vivo and in vitro test models and was also orally active. In most of the test systems it was found to be equipotent to papaverine.
The occurrence of reactive oxygen species (ROS), termed as prooxidants, is a characteristic of normal aerobic organisms. The term “reactive oxygen species” collectively denotes oxygen-centered radicals such as superoxide (O2·-)and hydroxyl (·OH), as well as nonradical species derived from oxygen, such as hydrogen peroxide (H2O2), singlet oxygen (1ΔgO2) and hypochlorous acid (HOC1). Radical reactions are central to the maintenance of homeostasis in biological systems. Radical species perform a cardinal role in many physiological processes such as cytochrome P450-mediated oxidative transformation reactions, a plethora of enzymic oxidation reactions, oxidative phosphorylation, regulation of the tone of smooth muscle, and killing of microorganisms.1–3 Excessive generation of free radicals can have deleterious biological consequences.4–6 Organisms are equipped with an armamentarium of defense systems, termed antioxidants in order to safeguard them against the onslaught of ROS.1–3,7 When the generation of prooxidants overwhelms the capacity of antioxidant defense systems oxidative stress ensues. This can cause tissue damage leading to pathophysiological events. ROS play a pivotal role in the action of numerous foreign compounds (xenobiotics). Their increased production seems to accompany most forms of tissue injury.4,5 Whether sustained and increased production of ROS is a primary event in human disease progression or a secondary consequence of tissue injury has been discussed.5,6 Whatever may be the case, the formation of free radicals has been implicated in a multitude of disease states ranging from inflammatory/immune injury to myocardial infarction and cancer.
The recent explosion of interest in the bioactivity of the flavonoids of higher plants is due, at least in part, to the potential health benefits of these polyphenolic components of major dietary constituents. This review article discusses the biological properties of the flavonoids and focuses on the relationship between their antioxidant activity, as hydrogen donating free radical scavengers, and their chemical structures. This culminates in a proposed hierarchy of antioxidant activity in the aqueous phase. The cumulative findings concerning structure-antioxidant activity relationships in the lipophilic phase derive from studies on fatty acids, liposomes, and low-density lipoproteins; the factors underlying the influence of the different classes of polyphenols in enhancing their resistance to oxidation are discussed and support the contention that the partition coefficients of the flavonoids as well as their rates of reaction with the relevant radicals define the antioxidant activities in the lipophilic phase.
Poncirin was isolated from water extract of the fruits of Poncirus trifoliata and metabolized by human intestinal bacteria. The inhibitory effect of poncirin and its metabolites by these bacteria on the growth of Helicobacter pylori (HP) was investigated. Among them, ponciretin (5,7-dihydroxy-4'-methoxyflavanone), the main metabolite most potently inhibited the growth of HP, with a minimum inhibitory concentration (MIC) of 10-20 microg/ml. However, poncirin and its metabolites except ponciretin did not inhibit the growth of HP, nor did they inhibit HP urease.
Immunoglobulin E (IgE) is the principal immunoglobulin involved in immediate hypersensitivities and chronic allergic diseases. The effect of an aqueous extract of Poncirus trifoliata (L) Raf. (Rutaceae) fruits (PTFE) on in vivo and in vitro IgE production was investigated. PTFE dose-dependently inhibited the active systemic anaphylaxis and serum IgE production induced by immunization with ovalbumin, Bordetella pertussis toxin and aluminum hydroxide gel. PTFE strongly inhibited interleukin 4 (IL-4)-dependent IgE production by lipopolysaccharide-stimulated murine whole spleen cells. In the case of U266 human IgE-bearing B cells, PTFE also showed an inhibitory effect on the IgE production. These results suggest that PTFE has an anti-allergic activity by inhibition of IgE production from B cells.
1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of Japanese whiskey after various aging periods in oak barrels was measured to evaluate the antioxidative effects of whiskey. The activity of the whiskey increased with the aging period with high correlation. The activity of various types of whiskey was measured and shown to be correlated to the potentiation of the GABAA receptor response measured in a previous paper. However, the fragrant compounds in the whiskey which potentiated the GABAA receptor response had low DPPH radical scavenging activity, while phenol derivatives had high radical scavenging activity. The whiskey was extracted by pentane. The aqueous part showed the scavenging activity, whereas the pentane part did not. Thus, both the DPPH radical scavenging activity and the potentiation of the GABAA receptor response increased during whiskey aging in oak barrels, but were due to different components. The whiskey protected the H2O2-induced death of E. coli more than ethanol at the same concentration as that of the whiskey. The changes that occurred in the whiskey during aging may be the reason aged whiskies are so highly valued.
Inhibition by ALOE extracts of L-dopa oxidation by mushroom-tyrosinase was examined. 2''- O-Feruloylaloesin and aloesin at concentrations of 0.4 microM showed inhibition of 27 and 30%, respectively. Lineweaver-Burk plots of the concentration of L-dopa in the absence and presence of 2''- O-feruloylaloesin, 0.4 and 0.8 microM, showed that this compound inhibits mushroom-tyrosinase noncompetitively. The K (i) value obtained was 8.5 x 10 (-5) M. 2''- O-Feruloylaloesin and aloesin contents were analyzed by a reversed-phase HPLC, and their seasonal variations were observed.
Isolation of melanogenesis inhibitors from Ponciri fructus
- Ar Son
- Jy Choi
- Ja Kim
- Cho
- Sh
- Xg Hua
- Sh Park
- Sr Chung
- Chung
- Dj Jang
- Jk Son
- Sh Lee
- Ar Son
- Jy Choi
- Ja Kim
- Cho
- Sh
- Xg Hua
- Sh Park
- Sr Chung
- Chung
- Dj Jang
- Jk Son
- Sh Lee
Antioxidative activity and physiological activity of some korean medicinal plants
- Hk Kim
- Ye Kim
- Jr Do
- Yc Lee
- By Lee
- Hk Kim
- Ye Kim
- Jr Do
- Yc Lee
- By Lee
Herb medicine pharmacology
- H C Kim
Kim
HC.
(2004)
Herb
medicine
pharmacology.
Screening for antioxidant activity of plant medicinal extracts
- S J Jung
- J H Lee
- H N Song
- N S Seong
- S E Lee
- N I Baek
Jung SJ, Lee JH, Song HN, Seong NS, Lee SE, Baek
NI. (2004) Screening for antioxidant activity of plant
medicinal extracts. J. Korean Soc. Appl. Biol. Chem.,
47, 135-140
Compartmentation of mono and sesquiterpene synthesis of essential oil in Poncirus trifoliata
- G Heinrich
- W Schultze
- R Wegner
The effects of emulsion-type sausages containing mulberry leaf and persimmon leaf power on lipid oxidant, nitrite, VBN and fatty acid composition
- Jr Lee
- Jd Jung
- Ki Lee
- Ym Song
- Sk Jin
- Is Kim
- Lee Hy Jh Kim
- Jr Lee
- Jd Jung
- Ki Lee
- Ym Song
- Sk Jin
- Is Kim
- Lee Hy Jh Kim
Lee JR, Jung JD, Lee KI, Song YM, Jin SK, Kim IS,
Kim HY, Lee JH. (2003) The effects of emulsion-type
sausages containing mulberry leaf and persimmon leaf
power on lipid oxidant, nitrite, VBN and fatty acid
composition. Korean J. Food. Sci. Ani. Resour., 23, 1-8
Illustrated flora of Korea 5th ed. Hyangmoonsa, Seoul, Korea
- T B Lee
Lee TB. (1993) Illustrated flora of Korea 5th ed.
Hyangmoonsa, Seoul, Korea, p.502-503
The separation and determination of flavonoid glycosides from Poncirus trifoliata Rafia and Citrus qaurantium L
- Tj Kim
- Jy No
- Js Ko
- Jw Rhee
- Tj Kim
- Jy No
- Js Ko
- Jw Rhee
Kim TJ, No JY, Ko JS, Rhee JW. (1989) The separation
and determination of flavonoid glycosides from Poncirus
trifoliata Rafia and Citrus qaurantium L. J. Anal. Sci.
Technol., 2, 301-307
Antioxidant activities and anticancer effect of Yuza
- Jm You
- Jb Park
- Ks Seoung
- Dy Kim
- Ik Hwang
- Jm You
- Jb Park
- Ks Seoung
- Dy Kim
- Ik Hwang
You JM, Park JB, Seoung KS, Kim DY, Hwang IK.
(2005) Antioxidant activities and anticancer effect of
Yuza. Food Sci. Ind., 38, 72-77
Screening of tyrosinase inhibitor from plants
- Sw Jung
- Nk Lee
- Han Sj Dw Kim
- Sw Jung
- Nk Lee
- Han Sj Dw Kim
Jung SW, Lee NK, Kim SJ, Han DW. (1995) Screening
of tyrosinase inhibitor from plants. Korean J. Food Sci.
Technol., 27, 891-896
Development of cosmetic material from Korean Crataegi fructus extract
- Bj An
- By Kang
- Jt Lee
- Bj An
- By Kang
- Jt Lee
An BJ, Kang BY, Lee JT. (2002) Development of
cosmetic material from Korean Crataegi fructus extract.
Korean J. Herbol., 17, 39-50