Content uploaded by Min-Soo Heu
Author content
All content in this area was uploaded by Min-Soo Heu on Aug 28, 2014
Content may be subject to copyright.
A preview of the PDF is not available
Food Component Characteristics of Cuttle Bone as a Mineral Source
Abstract
This study was conducted to get a knowledge on food components of cuttle bone (CB) as a food resource. The yields and ash contents of CB were about on whole cuttle fish and about on dry basis, respectively, The contents of heavy metal might not invoke health risk in using food resource, The major mineral of CB was calcium as about in content. The yields, proximate compositions, heavy metal and mineral contents were not significantly different between domestic and imported CB. Judging from X-ray diffraction pattern, most of calcium in CB was present as a form of calcium carbonate (CC), and scanning electron micrograph showed irregular form. Buffering capacity of CB showed strongly at pH 7, and its pattern was the same as shown in that of CC, And CB solution showed a very high degree in turbidity comparing to that of CC solution. The solubility of CB was superior to that of CC, but inferior to those of calcium powders on the market, It was concluded from above results that CB could be effectively utilized as a calcium source.
... Cuttlebone is rich in minerals, particularly calcium, because it mainly consists of over 90% of calcium carbonate and chitin (Cadman et al., 2012;Vajrabhaya et al., 2017). Sodium, phosphorus, magnesium, manganese, potassium, and iron are also present in trace amounts in cuttlebones (Cho et al., 2001;Yildirim et al., 2007). Different species of cuttlefish and squids were reported to possess several nutraceutical and therapeutic properties (Fatimah Zaharah & Rabeta, 2018;Hossain et al., 2018). ...
... Specifically, CBP2 showed the highest calcium content while CBP3 demonstrated the lowest. The calcium content of cuttlebones in this study was significantly lower than those observed by Cho et al. (2001), who found that the calcium contents of raw CBP from Korea and India were 22,341.4 mg/100 g and 22,233.6 mg/100 g, respectively (Table 3). ...
... Among the minerals analyzed in this study, iron showed the lowest contents in CBP. Cho et al. (2001) reported a similar result, that is, the iron contents of cuttlebones from cuttlefish caught from Korea and India were the lowest when compared with those of other minerals (Ca, Na, Mg and K) ( Table 3). The iron content of CBP in this study ranged from 0.08 mg/100 g sample to 0.18 mg/100 g sample, and no significant difference (p > .05) in this metal was noted among the three samples. ...
This study was aimed to investigate the potential use of pre‐treated cuttlebone powder (CBP) as an alternative calcium source by evaluating the physicochemical properties of CBP pre‐treated with distilled water (CBP1), 1% acetic acid (CBP2) and 2% sodium hydroxide (NaOH) solutions (CBP3). Proximate analysis revealed ash as the major component of all samples. Presence of phenols, flavonoids, alkaloids and glycosides were detected but tannins appeared to be absent in cuttlebones. Mineral analysis indicated that CBP was rich in calcium, which means the material may be considered as a potential calcium source for food products or calcium supplements. The bulk density of CBP2 was significantly lower (p<0.05) than those of CBP1 and CBP3, and the water holding capacity (WHC) of CBP1 was significantly higher (p<0.05) than those of CBP2 and CBP3. The high bulk density, water and oil holding capacities of CBP demonstrated its potential use as a functional food ingredient.
... 7 It is common knowledge that waste cuttlefish shells contain inorganic components comprising between 75 and 90 percent, the majority of which is calcium carbonate. 8 Calcium carbonate, which is found in cuttlefish shells, can be used to make better orthopedic materials. One such material is CHA. 9 Molecular modeling refers to the process of utilizing computational chemistry and graphical visualization tools to present three-dimensional images of chemical systems in order to investigate the structure and characteristics of molecules. ...
Background: Relapse occurs frequently, 70–90% of the time, and typically compromises the outcome of orthodontic therapy. Calcium carbonate (CaCO3), which is found in cuttlefish shells, can be used to make a better biomaterial. One example is carbonated hydroxyapatite, which is very similar to human bone tissue and can stop osteoclast activity on the pressure side of the retention phase. This is a factor in orthodontic relapse, which is when the bone doesn't remodel properly. In this study, a test was done to see if carbonated hydroxyapatite (CHA) could be used as an alternative material to stop orthodontic relapse. The test was based on how the RANK-RANKL, OPG, and TGF-β proteins interacted with each other. Method: CHA extracted from cuttlefish shells after 6 hours of calcination at 1000°C. RANK-RANKL, OPG, and TGF-β interactions were investigated in silico using molecular docking. Result: A cuttlefish shell extract containing CHA has the potential to be used as an alternate material to prevent orthodontic recurrence. CHA chemicals can disrupt the link between RANK and RANKL and enhance OPG and TGF-β expression. This induces enhanced proliferation, which increases the number of osteoblasts and osteoblast differentiation while decreasing the rate of osteoclast activity. Conclusion: Cuttlefish shell with CHA extract has the potential to be used as an alternative material to prevent orthodontic relapse.
... ancy tank and the hard structural component of the body, minimizing the efforts needed to keep the cuttlefish vertical in the water (Birchall, 1983). cuttlebone waste generated by the processing of cuttlefish (Fig. 1). As per our study, the cuttlebone comprises approximately 4.2%-7% of the total body weight. Thus, the estimated yield of cuttlebone Cho et. al., (2001) generated from the processing of cuttlefish in India should be approximately in metric tons per year as per the export of processed cuttlefish (Table 1). ...
catch is mostly composed of Sepia pharaonis, Sepia aculeata, Sepiella inermis and Sepia eliptica. Major cuttlefish landing was observed in Gujarat with 0.3 lakh tons followed by Maharashtra with 0.25 lakh tons. Cuttlebone availability Processing of cuttlefish in India for export or household needs only uses the body, including the meat and the head; thus, the cuttlebone is categorized as a byproduct of the processing waste. As per the landings of cuttlefish in India, there is abundant availability of Abstract C uttlebone is the calcareous internal shell of a cuttlefish composed of calcium carbonate in its aragonite polymorph followed by an organic compound in the form of β-chitin and minerals. The cuttlebone is categorized as a byproduct of processing waste. Therefore, the estimates of cuttlebone resulting from this process are very abundant. Due to such properties of cuttlebone, it can be utilized to fulfill the demand for calcium and such minerals and used as an antacid or as an adsorbent. Over the period, cuttlebone is being utilized traditionally as a natural marine drug, for the benefit of birds and reptiles, mold-making material and lime production by the people of India. Due to its physical and chemical characterization; availability and immense medicinal properties, it has gained the interest of many researchers and provides much insight into the pharmaceutical industry.
... Finally, the donax shell powder showed the highest crude fiber content followed by oyster shell and then cuttlebone powders. Cho et al., (2001) found that the ash content of cuttlebone was about 7.5% on the whole cuttlefish and about 90% on a dry basis. Each value is an average of three determinations. ...
... Ca), so the amount of Ca is well determined Hemmatti et al. (2018) (Tables 2 and 3). Moon-Lae et al. (2001) they found that the yields and ash contents of cuttlebone were about 7.5% on whole cuttlefish and about 90% on dry basis, respectively. The contents of heavy metal might not invoke health risk in using food resource. ...
... Hal ini berdampak pada berkembangnya agroindustri pengolahan hasil perikanan yang menyediakan berbagai olahan produk sotong, baik dalam bentuk segar, beku maupun produk turunan lainnya, namun dalam proses pengolahannya sotong hanya dimanfaatkan bagian badan yang meliputi daging hingga kepala, sedangkan cangkang dan jeroan dianggap sebagai limbah samping hasil pengolahan (Nurimala et al. 2018). Limbah cangkang sotong diketahui memiliki unsur anorganik mencapai 75-90% yang sebagian besarnya merupakan kalsium karbonat (Cho et al. 2001). ...
The increasing production of cuttlefish has been associated with the increasing of by-product waste particularly cuttlebone. Cuttlebone is known to contain an inorganic element in form of calcium carbonate
(CaCO3) which can be utilized as a source of calcium oxide (CaO) for hydroxyapatite synthesis. This study was aimed to determine the physicochemical characteristics of the cuttlebone and the optimum calcination temperature for CaO extraction and hydroxyapatite synthesis. This study was divided into three steps. Firstly, analysis of the cuttlebone physicochemical properties; secondly, extraction and characterization of the CaO with different calcination temperature (500°C, 600°C, 700°C for 6 hours); and thirdly, hydroxyapatite synthesis using a combination of hydrothermal method at 200°C 6 hours and different calcination treatments (800°C, 900°C, 1,000°C for 1 hour). The results showed that the cuttlebone contained moisture 3.54±0.11%,
lipid 0.32±0.19%, protein 4.78±0.23%, carbohydrate 5.29±0.02%, and ash 89.61±0.26. The main element of the ash was CaCO3 aragonite characterized by the high absorption at wavelengths of 1,795; 1,507;
1,083; 871; 713 and 700 cm-1. The calcination treatment of 700°C produced the highest amount of CaO. The hydroxyapatite produced with a combination of hydrothermal and calcination temperature 1,000°C
had calcium phosphate ratio (Ca/P) 1.66, crystalline level 90.10%, amorphous level 9.90% and particles morphology of rod-shaped.
... After a while, the Spirulina, Cyperus alternifolius and Orthotrichum rupestre grew in the aquarium and the snails feed on them. Furthermore a few pieces of cuttlebones were added to the aquarium as a supplementary source of calcium (Cho et al., 2001). The aquarium water temperature and pH were monitored regularly. ...
In this study a relatively large and open top aquarium was designed, constructed and introduced as a suitable habitat for nutrition, growth and development as well as for egg laying and breeding of Galba truncatula under laboratory conditions. The soil and water used in the aquarium were collected from the locality in which the snails were collected. The aquarium was placed in a laboratory with temperature of 18–32 ºC and relative humidity of 22–37% respectively, according to the season. The artificial light was controlled by a light timer, giving 12 h of light and 12 h of darkness. The snails were fed with dried lettuce leaves, Cyperus alternifolius (aquatic plant), Spirulina (algae), Orthotrichum rupestre (moss) and cuttlebone (a supplementary source of calcium). Approximately five weeks after the start of study, there was evidence of reproduction and success in rearing of G. truncatula by the appearance of eggs and small snails (0.1–0.5 mm) in the aquarium. In conclusion, large scale laboratory rearing of G. truncatula is a feasible task. The method may be improved by balancing the temperature and relative humidity as well as by optimizing the soil type, the water quality and the type of food.
... [24] A study reported that raw CB contains 0.05 ppm mercury, 0.52 ppm copper, 2.42 ppm zinc, 0.39 ppm lead, and 0.07 ppm cadmium and is not cytotoxic in vitro. [25] Zreiqat et al. [26] suggested that implant surfaces coated with Mg 2+ promote optimal osteogenesis and lead to the maintenance of nature and healthy bone. The Mg 2+ ion has an important role in integrins binding to their respective ligands. ...
Objective
To evaluate the cytotoxic and the proliferative effect of cuttlefish bone on MC3T3-E1 osteoblast cell line.
Materials and Methods
MC3T3-E1 cells were treated with 0.5, 1, 5, 25, 50, 100, or 200 μg/ml cuttlefish bone powder (CBP). Cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. This assay was also used to determine cell proliferation over 16 days of treatment with 0.5, 25, or 100 μg/ml CBP.
Results
CBP was not cytotoxic to MC3T3-E1 cells at any concentration. The percentage of cell viability in the 0.5–200 μg/ml CBP groups dose dependently decreased from 107.52 ± 11.03 to 92.48 ± 5.60%; however, the differences between the groups or the negative control group were not significant. At 16 days, 0.5, 25, and 100 μg/ml CBP groups showed 123.19 ± 10.07%, 126.02 ± 15.69%, and 133.33 ± 11.74% proliferation, respectively, that were significantly higher than that of the control group.
Conclusion
These results indicate that CBP promotes osteoblast proliferation and may be a potential material to increase the number of osteoblasts in a bone defect in the oral cavity.
... LCCLs . Cho et al. (2001a) Kim et al. (2003) , pH 12.9 ...
Clamshells, which comprise more than 50% of a clam’s weight, are a major byproduct of the clam industry and are
mainly composed of insoluble calcium carbonate. This study investigates the use of clamshells as a natural calcium
resource. Highly soluble powdered calcium lactate (LCCL) was prepared from the calcined powdered shells of littleneck clams (LCCP) using response surface methodology (RSM) to predict optimum conditions. These conditions, as derived from pH, solubility, and yield of 11 LCCLs manufactured according to the RSM model, were 1.80 M lactic acid and 1.13 M LCCP. The actual values of pH (6.98), solubility (93.99%), and yield 351.23%) under the optimized conditions were as predicted. The derived LCCL exhibited a strong buffering capacity in the range of pH 2.78-3.90 when combined with less than 2 mL of 1 N HCl. The ranges of calcium content and solubility of LCCL were 7.7-17.5 g/100 g and 96.6-98.9%, respectively. Fourier transform infrared spectroscopy (FT-IR) of the LCCL identified it as calcium lactate pentahydrate, and field emission scanning electron microscopy (FESEM) revealed an irregular and rod-like microstructure. These results confirm the potential use of clamshells, converted to highly soluble organic acid calcium, as an additive to enhance calcium content in food ingredients.
To facilitate the effective use of butter clam shell as a natural calcium resource, we determined the optimal conditions for calcium lactate (BCCL) preparation with high solubility using response surface methodology (RSM). The polynomial models developed by RSM for pH, solubility and yield were highly effective in describing the relationships between factors (P<0.05). Increased molar ratios of calcined powder (BCCP) from butter clam shell led to reduced solubility, yield, color values and overall quality. The critical values of multiple response optimization to independent variables were 1.75 M and 0.94 M for lactic acid and BCCP, respectively. The actual values (pH 7.23, 97.42% for solubility and 423.22% for yield) under optimization conditions were similar to the predicted values. White indices of BCCLs were in the range of 86.70–90.86. Therefore, organic acid treatment improved color value. The buffer-ing capacity of BCCLs was strong, at pH 2.82 to 3.80, upon the addition of less than 2 mL of 1 N HCl. The calcium content and solubility of BCCLs were 6.2–16.7 g/100 g and 93.6-98.5%, respectively. Fourier transform analysis of infrared spectroscopy data identified BCCL as calcium lactate pentahydrate, and the analysis of microstructure by field emission scanning electron microscopy revealed an irregular form.
ResearchGate has not been able to resolve any references for this publication.