The capillary zone electrophoretic (CZE) method for rapid quantitative and qualitative determination of ibotenic acid (IBO) and muscimol (MUS) in Amanita mushrooms naturally grown in Poland was developed. The investigations included the species of Amanita muscaria, Amanita pantherina and Amanita citrina, collected in southern region of Poland. The studied hallucinogenic compounds were effectively extracted with mixture of methanol and 1 mM sodium phosphate buffer at pH 3 (1:1v/v ) using ultrasound-assisted procedure. The obtained extracts were separated and determined by CZE utilizing a 25 mM sodium phosphate running buffer adjusted to pH 3 with 5% content of acetonitrile (v/v ). The calibration curves for both analytes were linear in the range of 2.5 - 7000 μg mL(-1) . The intra-day and inter-day variations of quantitative data were 1.0% and 2.5% RSD, respectively. The recovery values of analyzed compounds were over 87%. The identify of ibotenic acid and muscimol were confirmed by UV spectra, migration time and measurements after addition of external standard. This article is protected by copyright. All rights reserved.