Protective effect of Carica papaya L leaf extract against alcohol induced acute gastric damage and blood oxidative stress in rats. West Indian Med J

Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
The West Indian medical journal (Impact Factor: 0.33). 10/2008; 57(4):323-6.
Source: PubMed


The effects of Carica papaya leaf (CPL) aqueous extract on alcohol induced acute gastric damage and the immediate blood oxidative stress level were studied in rats. The results showed that gastric ulcer index was significantly reduced in rats pretreated with CPL extract as compared with alcohol treated controls. The in vitro studies using 2,2-Diphenyl-1-Picryl-Hydrazyl (DPPH) assay showed strong antioxidant nature of CPL extract. Biochemical analysis indicated that the acute alcohol induced damage is reflected in the alterations of blood oxidative indices and CPL extract offered some protection with reduction in plasma lipid peroxidation level and increased erythrocyte glutathione peroxidase activity. Carica papaya leaf may potentially serve as a good therapeutic agent for protection against gastric ulcer and oxidative stress.

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    • "It was reported that experimentally induced diabetic rats showed significant wound healing after treatment with the aqueous extract of the fruit of C. papaya [5]. The aqueous extract of the leaves of the plant showed significant protective effect against alcohol induced oxidative damage to the gastric mucosa in rats [6], and it was effective in healing induced wound in rats [7]. The aqueous extract of the leaves also exhibited the ability to inhibit tumor cell lines [8] and this activity was potentiated with phenolic compounds [9] [10]. "
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    ABSTRACT: The subchronic toxicity effect of the leaf extract of Carica papaya Linn. in Sprague Dawley (SD) rats was investigated in this study. The extract was prepared by dissolving the freeze dried extract of the leaves in distilled water and was administered orally to SD rats (consisted of 10 rats/sex/group) at 0 (control), 0.01, 0.14, and 2 g/kg body weight (BW) for 13 weeks. General observation,mortality, and food and water intake were monitored throughout the experimental period. Hematological and biochemical parameters, relative organ weights, and histopathological changes were evaluated.The study showed that leaf extract when administered for 13 weeks did not cause any mortality and abnormalities of behavior or changes in body weight as well as food and water intake.There were no significant differences observed in hematology parameters between treatment and control groups; however significant differences were seen in biochemistry values, for example, LDH, creatinine, total protein, and albumin. However, these changes were not associated with histopathological changes. In conclusion, the results suggested that daily oral administration of rats with C. papaya leaf extract for 13 weeks at a dose up to fourteen times the levels employed in traditional medicine practice did not cause any significant toxic effect.
    Full-text · Article · Oct 2014 · Evidence-based Complementary and Alternative Medicine
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    • "The studies of the antioxidant nutrients in C. papaya have led to the identification of the main compounds that differ in the different organs. Whole fruit extract contains ferulic, p-coumaric, and caffeic acid, carotenoids, and vitamin C that collectively protect human cells from oxidative stresses [4] and promote wound healing and skin repair [8, 9]; leaves extract contains folic acid, vitamins B12, A, and C, alkaloids, saponins, glycosides, tannins, and flavonoids [10] with anticancer activity [11, 12] and protection against the alcohol-induced oxidative damage to the gastric mucosa [13]; seeds extract contains different phenolic compounds, vanillic acid, and vitamin C with antioxidant [14–17] and anticancer [5] activities. Thus, C. papaya extracts may act as a synergistic therapeutic dietary supplement in patients with oxidative stress related diseases or could be added to formulations to promote wound healing. "
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    ABSTRACT: Carica papaya is widely used in folk medicine as herbal remedy to prevent, protect against, and cure several diseases. These curative properties are based on the presence in different parts of the plant of phytochemical nutrients with antioxidant effect. Seeds are the less exploited part; thus this study is aimed at assessing the antioxidant activities of the C. papaya seeds water extract against hydrogen peroxide (H2O2) oxidative stress in human skin Detroit 550 fibroblasts. C. papaya seeds water extract is not toxic and acts as a potent free radical scavenger, providing protection to Detroit 550 fibroblasts that underwent H2O2 oxidative stress. Data show that (i) the maximum protective effect is achieved by the simultaneous administration of the extract with 1 mM H2O2; (ii) the extract in presence of an oxidative stress does not increase catalase activity and prevents the release of cytochrome C and the inner mitochondrial transmembrane potential (Δψ m ) loss; (iii) the extract is more efficient than vitamin C to hamper the oxidative damage; (iv) the purified subfractions of the seeds water extract exert the same antioxidant effect of whole extract. In conclusion, C. papaya seeds water extract is potentially useful for protection against oxidative stress.
    Full-text · Article · Mar 2014 · Evidence-based Complementary and Alternative Medicine
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    • "Indran et al. have shown that there is a protective effect against gastric ulcers in rats [13] . Moreover, C. papaya flowers are known to have antibacterial activities [15] . "
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    ABSTRACT: To investigate the potential role of fresh Carica papaya (C. papaya) leaf extract on haematological and biochemical parameters and toxicological changes in a murine model. In total 36 mice were used for the trial. Fresh C. papaya leaf extract [0.2 mL (2 g)/mouse] was given only to the test group (18 mice). General behavior, clinical signs and feeding patterns were recorded. Blood and tissue samples were collected at intervals. Haematological parameters including platelet, red blood cell (RBC), white blood cell (WBC), packed cell volume (PCV), serum biochemistry including serum creatinine, serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic-pyruvic transaminase (SGPT) were determined. Organs for possible histopathological changes were examined. Neither group exhibited alteration of behavior or reduction in food and water intake. Similarly, no significant changes in SGOT, SGPT and serum creatinine levels were detected in the test group. Histopathological organ changes were not observed in either group of mice except in three liver samples of the test group which had a mild focal necrosis. The platelet count (11.33±0.35)×10(5)/µL (P=0.000 04) and the RBC count (7.97±0.61)×10(6)/µL (P=0.000 03) were significantly increased in the test group compared to that of the controls. However, WBC count and PCV (%) values were not changed significantly in the test group. The platelet count in the test group started to increase significantly from Day 3 (3.4±0.18×10(5)/µL), reaching almost a fourfold higher at Day 21 (11.3×10(5)/µL), while it was 3.8×10(5)/µL and 5.5×10(5)/µL at Day 3 and Day 21 respectively in the control. Likewise, the RBC count in the test group increased from 6×10(6)/µL to 9×10(6)/ µL at Day 21 while it remained near constant in the control group (6×10(6)/µL). Fresh C. papaya leaf extract significantly increased the platelet and RBC counts in the test group as compared to controls. Therefore, it is very important to identify those chemicals of C. papaya leaves as it can be recommended to be used as a medication to boost thrombopoiesis and erythropoiesis in humans and in animals in which these cell lineages have been compromised.
    Full-text · Article · Sep 2013 · Asian Pacific Journal of Tropical Biomedicine
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