Far-field superresolution imaging with dual-dye-doped nanoparticles

Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, P.O. Box 800-211, Shanghai 201800, China.
Optics Letters (Impact Factor: 3.29). 07/2009; 34(12):1831-3. DOI: 10.1364/OL.34.001831
Source: PubMed


We propose to achieve superresolution in far-field fluorescence imaging by use of silica nanoparticles codoped with Cy3 and Cy5 molecules at a controllable ratio. Owing to the short distances between the dye molecules confined in the nanoparticles, Förster resonant energy transfer can efficiently occur between the Cy3 and Cy5 molecules. Therefore, the Cy5 molecules can quench the fluorescence emission from the Cy3 molecules in the outer region of focal spot of the excitation beam when the excitation intensity is properly chosen, thereby enhancing the resolution of imaging.

  • [Show abstract] [Hide abstract]
    ABSTRACT: We theoretically demonstrate that the spatial resolution of stimulated emission depletion (STED) microscopy can be substantially enhanced without increasing the intensity of the STED beam. In our scheme, tiny nanobeads codoped with donor and acceptor molecules are used as fluorescent probes, in which Förster resonance energy transfer (FRET) can occur with an ~100% efficiency between the donors and acceptors. Enhancement of the depletion of acceptors in the nanobeads with the doughnut-shaped depletion beam can lead to an increase of FRET efficiency in the outer area of the excitation spot, which itself is used for deexciting donor molecules and, consequently, enhancing the optical resolution.
    No preview · Article · Dec 2010 · Optics Letters
  • [Show abstract] [Hide abstract]
    ABSTRACT: We propose to achieve far-field super-resolution imaging by using offset two-color one-photon (2C1P) excitation of reversible photoactivatable fluorescence proteins. Due to the distinctive photoswitching performance of the proteins, such as dronpa, the fluorescence emission will only come from the overlapped region of activation beam and excitation beam. The analysis solution of rate equation shows that the resolution of offset 2C1P microscope is "engineered" by laser power of excitation and activation beams and the power ratio between them. Superior lateral and transverse resolution is theoretically demonstrated compared with conventional fluorescence scanning microscopy.
    No preview · Article · May 2011 · Chinese Physics Letters
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Hybrid nanoparticles are designed and produced by a two-step procedure with the aimto obtain optically controlledmultifunctional nanomaterials. In particular, using a sol�gel method in alcoholic/water media, silica particles doped with 9-aminoacridine molecules are prepared with amean diameter of 31 nm, which preserves the fluorescent properties of the dye. In a second step, these nanoparticles are capped with a thin (7 nm-size) gold shell whose growth does not quench the emission of the dye as proven by steady-state and time-resolved fluorescence measurements. The careful choice of the organic dye and the control of the metal layer growth make possible to completely uncouple the fluorescence and the plasmon bands of gold. The selective photoexcitation of fluorescence or plasmon absorption, leading to heat release, has been tested on phospholipidic membranes loaded with the prepared hybrid particles. Under 400-nm irradiation fluorescence is activated, which is used to image the membranes; upon 650-nm irradiation only the gold layer absorbs and efficiently converts light into heat leading to a temperature increase of about 10 �C in the surrounding medium which is responsible for the alteration of the membrane architecture.
    Full-text · Article · Dec 2011 · The Journal of Physical Chemistry C
Show more