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Inventi Rapid: Molecular Pharmacology Vol. 2014, Issue 2
[ISSN 0976-3856]
2014 pmp 277, CCC: $10 © Inventi Journals (P) Ltd
Published on Web 20/03/2014, www.inventi.in
REVIEW ARTICLE
INTRODUCTION
Liver fibrosis or scaring of liver is a complex, dynamic
change in normal wound healing response to different
fibrogenic stimuli leading to activation and trans
differentiation of hepatic stellate cells to myofibroblasts
which leads to excessive synthesis and deposition of
extracellular matrix components like collagen (type I and
type III) accompanied by distortion of normal hepatic
vasculature, hepatocyte dysfunction, irreversible liver
damage, complications and result in death. [1] Cirrhosis
occurs due to the necrosis of liver cells followed by fibrosis
and nodule formation. Impairment in liver function and
structure leads to impaired liver blood flow and function.
This derangement produces the clinical features of portal
hypertension. Cirrhosis represents the common pathway
for chronic liver diseases. In 1826, the term cirrhosis was
introduced by Laennec. It is derived from the Greek term
scirrhus which refers to the orange or tawny surface of the
liver. Cirrhosis is defined as a diffuse hepatic process
characterized by fibrosis and the conversion of normal liver
architecture into structurally abnormal nodules. The
progression of liver injury to cirrhosis may occur over
weeks to years. Patients with hepatitis C may have chronic
hepatitis for as long as 40 years before progressing to
cirrhosis. Many forms of liver injury are marked by fibrosis,
which is defined as an excess deposition of the components
of the extracellular matrix (collagens, glycoproteins and
proteoglycans) within the liver. This response to liver
injury is potentially reversible while in most patients,
cirrhosis is not a reversible process. In addition to fibrosis,
the complications of cirrhosis include portal hypertension,
ascites, hepatorenal syndrome and hepatic encephalopathy.
A poor correlation exists between histologic findings of
cirrhosis and the clinical picture. Some patients with
cirrhosis are completely asymptomatic and have a
reasonably normal life expectancy while some individuals
1Aksharpreet Institute of Pharmacy, Adi Shankaracharya Shikshan Vikas
Sankul, Jamnagar - Okha State Highway, Lakhabaval Road, Jamnagar-
361006, Gujarat, India.
E-mail: manojsuva_0211@yahoo.co.in
*Corresponding author
have severe symptoms of end-stage liver disease and
limited chance for survival. Common signs and symptoms
may arise from decreased hepatic synthetic function
(coagulopathy), decreased detoxification capabilities of the
liver (hepatic encephalopathy) or portal hypertension
(variceal bleeding). [2]
EPIDEMIOLOGY
In the United States, chronic liver disease and cirrhosis
result in about 35,000 deaths each year. In the United
States, cirrhosis is the 9th leading cause of death and
account for 1.2% of all US deaths. Fulminant hepatic failure
(FHF) accounts for 2000 additional deaths every year.
Causes of FHF includes viral hepatitis (Hepatitis A and B),
drugs (Acetaminophen), toxins like Amanita phalloides,
autoimmune hepatitis and Wilson disease. Patients with
the FHF have a 50-80% mortality rate unless liver
transplantation. [2] Different causes of cirrhosis are shown
in Table 1.
PATHOPHYSIOLOGY
The liver plays an important role in synthesis of proteins
like albumin, clotting factors, complement factors and
detoxification and storage of vitamin A. It participates in
the metabolism of lipids and carbohydrates. Cirrhosis is
often followed by hepatitis and steatosis (fatty liver)
independent of the cause. If the cause is resolved at this
stage, the changes are completely reversible. In cirrhosis,
scar tissue development replaces normal parenchyma and
blocks the portal flow of blood to organ and affects the
normal function. Research shows the important role of the
stellate cell in the development of cirrhosis which generally
stores vitamin A. Hepatic parenchyma damage due to the
inflammation, activate stellate cell and it increases fibrosis
and obstructs the blood flow in the circulation. The
formation of fibrous tissue bands separate hepatocyte
nodules which replace the entire liver architecture.
Chronic injury to the liver results in inflammation, necrosis
and subsequently fibrosis as shown in Figure 1.
Fibrosis is initiated by activation of the stellate cells and
Kupffer cells, damaged hepatocytes and activated platelets
are also involved. Stellate cells are activated by various
A Brief Review on Liver Cirrhosis: Epidemiology, Etiology,
Pathophysiology, Symptoms, Diagnosis and Its
Management
Manoj A Suva1*
Abstract:
Cirrhosis is characterized by the formation of regenerative nodules in liver parenchyma surrounded by fibrous septa
due to chronic liver injury.
Cirrhosis occurs due to necrosis of liver cells followed by fibrosis and nodule formation. The liver
structure
becomes abnormal and interferes with liver blood flow and function and leads to portal hypertension and impaired
hepatocytes function. Chronic liver diseases represent a significant health problem across the globe with
liver cirrhosis. The
exact prevalence
of cirrhosis worldwide is still unknown as the clinical spectrum ranges from indolent, asymptomatic to
complete hepatic decompensation. Diagnosis of cirrhosis includes
serological test, histological test, transientelastography and
radio techniques like ul
trasonography, computerised tomography scan and magnetic resonance imaging. Ursodeoxycholic acid
is used for treatment of primary biliary cirrhosis. For treatment of autoimmune hepatitis prednisone and azathioprine are used.
For hepatitis B and C treatment
interferon and antiviral agents are used. For treatment of hemochromatosis phlebotomy is used.
For treatment of wilson disease, trientine and zinc are used. Liver transplantation is main curative option for treatment of liver
cirrhosis, but it possesses significant risk to the patient.
1
Inventi Rapid: Molecular Pharmacology Vol. 2014, Issue 2
[ISSN 0976-3856]
2014 pmp 277, CCC: $10 © Inventi Journals (P) Ltd
Published on Web 20/03/2014, www.inventi.in
REVIEW ARTICLE
cytokines and their receptors, reactive oxygen
intermediates, autocrine signals and paracrine signals. In
the early stage of activation, the swollen stellate cells loses
retinoids with up regulation of receptors for fibrogenic and
proliferative cytokines like transforming growth factor β1
(TGF-β1) (potent fibrogenic mediator) and platelet derived
growth factor (PDGF). Inflammatory cells causes fibrosis
due to cytokine secretion. Collagens (type I and III) and
fibronectin replaces the normal matrix in the space of
Disse. Sub endothelial fibrosis leads to loss of the
endothelial function and impairs liver function.
Collagenases (matrix metalloproteinases) are able to
degrade collagen but inhibited by tissue inhibitors of
metalloproteinases (TIMPs) which level is increased in
liver fibrosis. In initial stage, liver fibrosis is reversible
when the inflammation is reduced by either suppressing or
eliminating viruses. The pathologic features of cirrhosis
includes regenerating nodules separated by fibrous septa
and loss of the normal lobular architecture within the
nodules which leads to decreased blood flow throughout
the liver. Spleen congestion leads to hypersplenism and
increased sequestration of platelets as shown in Figure 2. [4-
6] Two types of cirrhosis are described based on the
underlying cause (a) Micro nodular cirrhosis in which
regenerating nodules size is about less than 3 mm and the
involvement of entire liver and often caused by alcohol
induced damage or biliary tract disease. (b) Macro nodular
cirrhosis in which the variable size nodules are formed and
normal acini is seen within the larger nodules and it is
often associated with chronic viral hepatitis. [3]
Macroscopically, in the initial stage the liver enlarged
and as disease progresses it becomes smaller. Liver surface
becomes irregular with firm consistency and the color is
yellow if it associates with steatosis. There are three
macroscopic types depending on the size of the nodules:
micro nodular, macro nodular and mixed cirrhosis. In
micro nodular form (Laennec's cirrhosis or portal
cirrhosis) regenerating nodules size is less than 3 mm. In
Table 1: Etiology
Autoimmune
Autoimmune Hepatitis
Viral/infectious
a. Hepatitis B
b. Hepatitis C
c. Schistosomiasis
Metabolic
a. Alcohol
b. Toxins, medications
c. Hereditary hemochromatosis
d. Wilson’s disease
e. Non alcoholic steatohepatitis
f. Insulin resistance
Cholestatic
a. Primary biliary cirrhosis
b. Primarysclerosing cholangitis
c. Biliary atresia
d. Secondary biliary cirrhosis
Vascular
a. Right heart failure
b. Budd–Chiari syndrome
c. Alpha-1-antitrypsin deficiency
d. Sarcoidosis
e. Cystic fibrosis
Activation of the stellate cell is followed by proliferation of fibroblasts and
the deposition of collagen
Figure 1:
Pathogenesis of fibrosis [3]
Figure 2:
Pathology of cirrhosis
2
Inventi Rapid: Molecular Pharmacology Vol. 2014, Issue 2
[ISSN 0976-3856]
2014 pmp 277, CCC: $10 © Inventi Journals (P) Ltd
Published on Web 20/03/2014, www.inventi.in
REVIEW ARTICLE
macro nodular cirrhosis (post-necrotic cirrhosis), the
nodules size is larger than 3 mm. The mixed cirrhosis
consists of nodules with different sizes. Cirrhosis is defined
by its pathological features: (a) the presence of
regenerating nodules of hepatocytes and (b) the presence
of fibrosis or the deposition of connective tissue between
these nodules. The pattern of fibrosis depends upon the
underlying causes. The fibrosis lead to normal tissues
destruction in the liver including sinusoids, the space of
Disse and vascular structures which lead to alteration in
resistance to blood flow in the liver and portal
hypertension. [7] Different entities injured the liver in
different ways causing specific abnormalities. (In chronic
hepatitis B- infiltration of the liver parenchyma with
lymphocytes is seen). [7] In cardiac cirrhosis erythrocytes
are present and fibrosis in the tissue surrounding the
hepatic veins is seen. [8] In primary biliary cirrhosis fibrosis
is seen around the bile duct. The granulomas are also
present and in alcoholic cirrhosis neutrophils infiltrates in
liver. [7, 9]
SYMPTOMS AND COMPLICATIONS OF CIRRHOSIS
In early stage of cirrhosis there are usually no symptoms.
Progressive condition it causes symptoms like Loss of
appetite, Tiredness, Nausea, Weight loss, abdominal pain,
Spider-like blood vessels, severe itching and various
complications are as follows:
1. Impaired metabolic and endocrine functions: Jaundice
2. Splenomegaly due to portal hypertension.
3. Haematological derangements such as
thrombocytopenia.
4. Gastrointestinal varices.
5. Ascites a severe complication due to portal
hypertension.
6. Spontaneous bacterial peritonitis.
7. Hepatocelluar carcinoma.
8. Hepatic encephalopathy.
9. Hyponatremia.
10.Hepatorenal syndrome.
11.Spider angiomata due to decreased oestradiol
degradation in liver. [10]
DIAGNOSIS [3, 10-12]
Serological Test
Aspartate aminotransferase (AST), Alanine transaminase
(ALT), Alkaline phosphatase (ALP), bilirubin, prothrombin
time, Gamma-glutamyl transpeptidase, albumin,
immunoglobulins mainly IgG, creatinine level, sodium level,
Low sodium indicates severe liver disease due to excessive
diuretic therapy or defective free water clearance. Albumin
level decreases below 28 g/l, serum creatinine elevated
concentration increased above 130 μmol/l and the
prothrombin time is prolonged.
Histological Test
Liver biopsy is considered as gold standard for diagnosis
and sequential histological grading of fibrosis and to
confirm the type and severity of liver disease. Stains are
required for copper and iron measurement to confirm
diagnosis of Wilson’s disease or iron overload and
immunocytochemical stains detects viruses, bile ducts and
angiogenic structures.
Radio Techniques
1. Ultrasound Examination
To detect changes in size, shape of the liver and to detect
hepatocellular carcinoma. Fatty change and fibrosis
produces high level of echogenicity. In cirrhosis, there may
be distortion of the arterial vascular architecture and
marginal nodularity of the liver surface. The patency of the
portal and hepatic veins are evaluated. Elastography is
used for diagnosis and follow up monitoring to avoid liver
biopsy.
2. Computerized Tomography Scan (CT Scan)
Arterial phase contrast enhanced scans are important in
the detection of hepatocellular carcinoma. Figure 3 shows
hepatosplenomegaly and collateral vessels enlargement
Figure 3:
Irregular lobulated liver
Figure 4:
Cirrhosis with a patent portal vein and no space occupying
lesion
3
Inventi Rapid: Molecular Pharmacology Vol. 2014, Issue 2
[ISSN 0976-3856]
2014 pmp 277, CCC: $10 © Inventi Journals (P) Ltd
Published on Web 20/03/2014, www.inventi.in
REVIEW ARTICLE
below the anterior abdominal wall (with arrows) due to
portal hypertension and Figure 4 shows dilated collaterals
in liver disease.
3. Endoscopy
For detection and treatment of portal hypertensive
gastropathy and varices.
4. Magnetic Resonance Imaging (MRI) Scan
For diagnosis of benign tumours (haemangiomas).
Magnetic resonance angiography demonstrates the
vascular anatomy and Magnetic resonance
cholangiography shows the biliary tree.
Transient Elastography
Scoring systems in cirrhosis is shown in Table 2.
MANAGEMENT OF CIRRHOSIS
Nutrition and Exercise
Many patients complain of anorexia, which may be due to
direct compression of ascites on the gastrointestinal tract.
Patients should receive adequate calories and protein in
diets. In 2010, American Association for the Study of Liver
Diseases and the American College of Gastroenterology
suggested guidelines for alcoholic liver disease and
recommend aggressive treatment of protein calorie
malnutrition in alcoholic cirrhosis patients. Multiple
feedings per day, Regular exercise including walking and
swimming to prevent inactivity and muscle wasting.
Debilitated patients get benefit from formal exercise
program supervised by a physician. [13] Specific therapies
are needed in liver diseases to prevent or treatment of the
development of cirrhosis. Prednisone and azathioprine
used for autoimmune hepatitis, phlebotomy used for
hemochromatosis, interferon and other antiviral agents
used for hepatitis B and C, ursodeoxycholic acid used for
primary biliary cirrhosis and trientine and zinc used for
Wilson disease. These therapies become less effective if
chronic liver disease evolves into cirrhosis. Once cirrhosis
develops, treatment is aimed at the management of
complications like variceal bleeding, ascites and hepatic
encephalopathy. Zinc deficiency commonly is observed in
patients with cirrhosis. Treatment with zinc sulfate at 220
mg orally twice daily may improve dysgeusia, muscle
cramps and as a adjunctive therapy for hepatic
encephalopathy. Pruritus is seen in cholestatic liver
diseases (primary biliary cirrhosis) and noncholestatic
chronic liver diseases (hepatitis C). Cholestyramine is used
for the treatment of pruritus in liver disease but care
should be taken to avoid co administration of
Cholestyramine with any other medication to avoid
impairment in gastro-intestinal absorption. Other drugs for
treatment of pruritus include antihistamines
(diphenhydramine, hydroxyzine), ursodeoxycholic acid,
ammonium lactate skin cream, doxepin and rifampin and
Naltrexone (an opioid antagonist). Patients with severe
pruritus may require ultraviolet light therapy or
plasmapheresis. Some patients with chronic cholestasis or
primary biliary cirrhosis and patients receiving
corticosteroids for autoimmune hepatitis require calcium
and vitamin D supplementation or use of
aminobisphosphonate (alendronate sodium).
Vaccination
Patients with chronic liver disease should receive
vaccination to protect against hepatitis A and as a
protective measure, vaccination against influenza and
pneumococci.
Analgesics
The use of analgesics in patients with cirrhosis can be
problematic. Most hepatologists permits the use of
acetaminophen doses of up to 2000 mg/day in patients
with cirrhosis. NSAID use in patients with cirrhosis may
cause gastrointestinal bleeding. Patients with cirrhosis are
at risk for NSAID induced renal insufficiency because of
prostaglandin inhibition and impairment in renal blood
flow. Opiate analgesics must be used with caution in
Table 2: Scoring Systems in Cirrhosis [3]
(I) Modified Child’s–Pugh Classification
Score
1
2
3
Ascites
None
Mild
Moderate/severe
Encephalopathy
None
Mild
Marked
Bilirubin (μmol/L)
< 34
34–50
> 50
Albumin (g/L)
> 35
28–35
< 28
Prothrombin time
(seconds over normal)
< 4
4–6
> 6
Add Above Scores for Your Patient for Survival Figures Below
Grade (scores)
% Survival
1 year
5 years
10 years
Child’s A (< 7)
82
45
25
Child’s B (7–9)
62
20
7
Child’s C (10+)
42
20
0
(II) Model of End Stage Liver Disease (MELD)
3.8 * LN bilirubin in (mg/dL) + 9.6 * LN creatinine in (mg/dL) + 11.2 * LN (INR) + 6.4
LN- Natural logarithm, INR- International normalized ratio, MELD scores (with no complications): 1 year survival 97% (score<10), 70% (score 30-40)
4
Inventi Rapid: Molecular Pharmacology Vol. 2014, Issue 2
[ISSN 0976-3856]
2014 pmp 277, CCC: $10 © Inventi Journals (P) Ltd
Published on Web 20/03/2014, www.inventi.in
REVIEW ARTICLE
patients with hepatic encephalopathy otherwise it may
worsen underlying mental function.
Drug Hepatotoxicity in the Patient with Cirrhosis
Medications associated with drug-induced liver disease
include NSAIDs, Isoniazid, Valproic acid, Erythromycin,
Amoxicillin/clavulanate, Ketoconazole, Chlorpromazine
and Ezetimibe. Statins are frequently associated with mild
elevations of alanine aminotransferase level and should be
used safely in patients with chronic liver disease.
Other Drugs
An amino glycoside antibiotic causes nephrotoxicity in
patients with cirrhosis and should be avoided. Low dose
estrogens and progesterone appear to be safe in the setting
of liver disease. [2]
Liver Transplantation
Liver transplantation has emerged as an important strategy
in the management of patients with cirrhosis. Patients
should be referred for consideration for liver
transplantation after the first signs of hepatic
decompensation. Advances in surgical technique, organ
preservation and immunosuppression have improved the
postoperative survival. In the early 1980s, the percentage
of patients surviving 1 year and 5 years after liver
transplant were 70% and 15% respectively. Now, patients
with 1-year survival rate are about 85-90% and a 5-year
survival rate of higher than 70%. Quality of life after liver
transplant is good or excellent in most cases. [2]
CONCLUSION
Cirrhosis represents the common histologic pathway for a
variety of chronic liver diseases. The damage to
hepatocytes causes impairment in liver functions. Cirrhosis
is mainly diagnosed by liver biopsy and other serological
laboratory test and radio techniques. Prednisone and
azathioprine used for autoimmune hepatitis, interferon and
other antiviral agents used for hepatitis B and C,
phlebotomy used for hemochromatosis, ursodeoxycholic
acid used for primary biliary cirrhosis and trientine and
zinc used for Wilson disease and liver transplantation.
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Cite this article as: Manoj A Suva. A Brief Review on
Liver Cirrhosis: Epidemiology, Etiology,
Pathophysiology, Symptoms, Diagnosis and Its
Management. Inventi Rapid: Molecular Pharmacology,
2014(2): 1-5, 2014.
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