Modulation of human dermal microvascular endothelial cells by Sarcoptes scabiei in combination with proinflammatory cytokines, histamine, and lipid-derived biologic mediators

Department of Pathology, Wright State University, Dayton, OH 45435, USA.
Cytokine (Impact Factor: 2.66). 07/2009; 47(2):103-11. DOI: 10.1016/j.cyto.2009.05.008
Source: PubMed


The ectoparasitic mite, Sarcoptes scabiei, produces molecules that depress initiation of host inflammatory and immune responses. Some of these down-regulate expression of adhesion molecules or secretion of chemokines or cytokines on and by cultured dermal endothelial cells (HMVEC-D). This study was undertaken to determine if the response of HMVEC-D to scabies is altered in the presence of various proinflammatory cytokines (tumor necrosis factor alpha and interleukins 1alpha, 1beta and 6), histamine, and lipid-derived mediators (prostaglandins D2 and E2, leukotriene B4, platelet activation factor) that likely occur in scabietic lesions in vivo. Scabies extract down-regulated the TNFalpha-induced expression of VCAM-1 by HMVEC-D and this down-regulation still occurred in the presence of the other proinflammatory cytokines, histamine or the lipid-derived mediators. Scabies inhibited the IL-1alpha and IL-1beta-induced secretion of IL-6, while a combination of scabies and histamine or LTB4 reduced the TNFalpha-induced secretion of IL-6. Scabies extract inhibited secretion of IL-8. Histamine, PGD2, PGE2, LTB4, PAF, and IL-6 alone had no effect on this inhibition, but the scabies-induced inhibition of IL-8 secretion was reduced in a dose-dependent fashion in the presence of IL-1alpha and IL-1beta.

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    Preview · Article · Aug 2013 · PLoS ONE
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    • "As in most of parasitosis, the severity of sarcoptic mange and the precise understanding of the host–parasite relationships depend, among other factors, on the parasitic load (Arlian et al., 1990; Davis and Moon, 1990). Recent studies provided evidences that mange modulates immunological defences (Kuhn et al., 2008; Elder et al., 2009; Fischer et al., 2009; Sarasa et al., 2010). The identification of the engaged molecules is still an ongoing process but it would be reasonable to expect the mite load, rather than lesions, to be a key factor of such physiological processes. "
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    • "effort) in mangy individuals (Forbes, 1993; Perrin et al., 1996) or a direct consequence of the impact of parasitism on host physiology and gonads (Baudoin, 1975). Recent studies provided direct and indirect evidences that mange modulates numerous physiological processes of the host, such as antioxidant (Dimri et al., 2008; Camkerten et al., 2009) and immunological defenses (Kuhn et al., 2008; Elder et al., 2009; Fischer et al., 2009; Sarasa et al., 2010a). The identification of the engaged molecules is still an ongoing process. "
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